Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,101,728 (G)7→8 coding (39/984 nt) kdgT → 2‑keto‑3‑deoxy‑D‑gluconate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,101,7211.G96.4% 404.2 / 9.2 112coding (32/984 nt)kdgT2‑keto‑3‑deoxy‑D‑gluconate transporter
Reads supporting (aligned to +/- strand):  ref base . (2/2);  new base G (54/54);  total (56/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

Only 100 of 131 total aligned reads displayed.

CATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGA  >  NC_000913/4101622‑4101816
                                                                                                    |                                                                                                
cATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAGTATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATccc                                                                                                   <  1:929637/100‑1 (MQ=255)
cATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATccc                                                                                                   >  1:403499/1‑100 (MQ=255)
cATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATccc                                                                                                   >  2:2492408/1‑100 (MQ=255)
cATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGCGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATccc                                                                                                   >  2:2321362/1‑100 (MQ=255)
 aTCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑g                                                                                                >  2:2586389/1‑100 (MQ=255)
 aTCAACACTGATGCTCCATTGAGGAATTACGCAACAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑g                                                                                                >  1:1905901/1‑100 (MQ=255)
   cAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑ggg                                                                                              >  1:1160444/1‑100 (MQ=255)
      cacTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑gggggg                                                                                           <  1:1321432/100‑1 (MQ=255)
      cacTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑gggggg                                                                                           <  2:2345828/100‑1 (MQ=255)
      cacTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑gggggg                                                                                           >  2:609223/1‑100 (MQ=255)
         tGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑gggggggga                                                                                        <  1:1514038/100‑3 (MQ=255)
          gATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑ggggggggat                                                                                       >  2:2750506/1‑97 (MQ=255)
            tGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑ggggggggatgn                                                                                     <  1:2088840/100‑6 (MQ=255)
             gCTCCATTGATGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGgatgat                                                                                     >  2:2460484/1‑100 (MQ=255)
             gCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGgatgat                                                                                     >  1:1466288/1‑100 (MQ=255)
              cTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGatgatg                                                                                    >  2:414240/1‑100 (MQ=255)
                ccATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCt                                                                                  >  2:1693431/1‑100 (MQ=255)
                ccATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCt                                                                                  >  2:1907152/1‑100 (MQ=255)
                ccATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCt                                                                                  <  2:728078/100‑1 (MQ=255)
                  aTTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCg                                                                                <  2:1402813/100‑1 (MQ=255)
                  aTTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCg                                                                                <  1:147551/100‑1 (MQ=255)
                    tGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTc                                                                              >  1:412895/1‑100 (MQ=255)
                     gAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTcc                                                                             >  1:441863/1‑100 (MQ=255)
                      aGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTccc                                                                            <  2:2710443/100‑1 (MQ=255)
                      aGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTccc                                                                            <  1:240841/100‑1 (MQ=255)
                      aGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTccc                                                                            <  2:108160/100‑1 (MQ=255)
                       ggAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCg                                                                           >  2:1853444/1‑100 (MQ=255)
                            tACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTAtt                                                                      >  1:221849/1‑100 (MQ=255)
                             aCGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTc                                                                     <  1:1672033/100‑1 (MQ=255)
                             aCGCATCAGCCCTTAAAAATATGCCGACAGGCGTTGGGAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑GGGGGGGATGATGCTCGTCCCGCTATTc                                                                     >  2:2807392/1‑99 (MQ=255)
                              cGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTcc                                                                    >  2:1782126/1‑100 (MQ=255)
                              cGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTcc                                                                    >  1:713480/1‑100 (MQ=255)
                                cATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCtt                                                                  >  2:1310732/1‑100 (MQ=255)
                                cATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTg                                                                 >  2:181038/1‑100 (MQ=255)
                                  tCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTgg                                                                >  1:592569/1‑100 (MQ=255)
                                   cAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGgc                                                               <  1:2586367/100‑1 (MQ=255)
                                      cccTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGgcgc                                                             >  1:2020901/1‑99 (MQ=255)
                                      cccTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCa                                                            <  1:466450/100‑1 (MQ=255)
                                       tcTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCAc                                                           <  2:488061/99‑1 (MQ=255)
                                       ccTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCAc                                                           <  2:1008775/100‑1 (MQ=255)
                                       ccTTAAAAATATGCCAACAGGTGATGGAAAGGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCAc                                                           <  2:2820867/100‑1 (MQ=255)
                                        cTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGACAATCCCGGGGGGGGGATGATGCTCGTCGCGGTATTCCTTGgcg                                                              >  1:2632610/1‑97 (MQ=255)
                                        cTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCAca                                                          >  1:488059/1‑99 (MQ=255)
                                        cTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACt                                                          >  2:1891638/1‑100 (MQ=255)
                                          tAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACtgt                                                        >  1:2421399/1‑100 (MQ=255)
                                             aaaTATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGcc                                                     <  2:1160449/100‑1 (MQ=255)
                                               atatGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCcac                                                   <  2:885027/100‑1 (MQ=255)
                                                tatGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCcaca                                                  >  2:1297194/1‑100 (MQ=255)
                                                  tGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACAcc                                                >  2:312444/1‑100 (MQ=255)
                                                  tGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACAcc                                                <  2:2751829/100‑1 (MQ=255)
                                                  tGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACAcc                                                <  1:1891624/100‑1 (MQ=255)
                                                     cGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTtc                                             <  2:594770/100‑1 (MQ=255)
                                                          ggTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGcc                                        >  1:1079981/1‑100 (MQ=255)
                                                            tGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCgg                                      >  2:2845302/1‑100 (MQ=255)
                                                             gATGGAAATGCGGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCggg                                     >  2:1452998/1‑100 (MQ=255)
                                                             gATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCggg                                     <  1:1093470/100‑1 (MQ=255)
                                                             gATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCggg                                     <  2:1106168/100‑1 (MQ=255)
                                                               tGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCggggg                                   >  2:2479496/1‑100 (MQ=255)
                                                               tGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCggggg                                   >  2:282869/1‑100 (MQ=255)
                                                                  aaaTGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCgg                                <  2:2542925/100‑1 (MQ=255)
                                                                   aaTGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCggg                               <  1:2368450/100‑1 (MQ=255)
                                                                   aaTGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCggg                               >  1:112063/1‑100 (MQ=255)
                                                                   aaTGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCggg                               <  1:312443/100‑1 (MQ=255)
                                                                    aTGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCgggg                              >  1:36675/1‑100 (MQ=255)
                                                                    aTGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCgggg                              <  2:1441367/100‑1 (MQ=255)
                                                                     tGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGTGGCgggg                              >  2:1978323/1‑100 (MQ=255)
                                                                     tGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCgggg                              >  1:2604333/1‑99 (MQ=255)
                                                                     tGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGa                             <  2:537894/100‑1 (MQ=255)
                                                                     tGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGa                             <  2:1060632/100‑1 (MQ=255)
                                                                     tGCAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGa                             >  1:137813/1‑100 (MQ=255)
                                                                     tGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGaa                            <  2:2422263/100‑1 (MQ=255)
                                                                       cAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGaaa                           >  2:762675/1‑100 (MQ=255)
                                                                       cAGATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGaaa                           >  1:1622240/1‑100 (MQ=255)
                                                                         gATAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAata                         >  2:1267160/1‑100 (MQ=255)
                                                                           tAAAACGCTCNATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACCCCTTCTCGCCGGGGGCGGGGAAata                         >  2:1130883/1‑98 (MQ=255)
                                                                           tAAAACGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATAtt                       <  2:2125837/100‑1 (MQ=255)
                                                                            aaaaCGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATAttt                      <  1:1738923/100‑1 (MQ=255)
                                                                             aaaCGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATAtttt                     >  1:447319/1‑100 (MQ=255)
                                                                              aaCGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTg                    <  2:2248591/100‑1 (MQ=255)
                                                                              aaCGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTg                    <  2:1547122/100‑1 (MQ=255)
                                                                               aCGCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTgg                   <  2:1151480/100‑1 (MQ=255)
                                                                                 gCTCGATTGAGAAAATCCCGGGGGGGGGTGATGCTCGTACCGCTATACCTCGGCGTCATATGCCAACCCTTCTTGGCGGGGGCGTGGAGat                          >  1:2678497/1‑91 (MQ=255)
                                                                                 gCTCGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGAt                 <  1:695428/100‑1 (MQ=255)
                                                                                    cGATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCAt              >  2:511139/1‑100 (MQ=255)
                                                                                     gATTGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCAtt             >  1:2298536/1‑100 (MQ=255)
                                                                                       ttGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCa           >  1:2850549/1‑100 (MQ=255)
                                                                                        tGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCAc          <  2:2152749/100‑1 (MQ=255)
                                                                                        tGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCAc          >  1:210728/1‑100 (MQ=255)
                                                                                        tGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCAc          >  1:2881865/1‑100 (MQ=255)
                                                                                        tGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCAc          >  1:498900/1‑100 (MQ=255)
                                                                                        tGAGAAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCAc          <  2:2113752/100‑1 (MQ=255)
                                                                                         gagaAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCAcc         >  1:1027265/1‑100 (MQ=255)
                                                                                         gagaAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCAcc         <  2:1723165/100‑1 (MQ=255)
                                                                                          agaAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCa        >  1:2167416/1‑100 (MQ=255)
                                                                                          agaAAATCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTACACGCCGGGGGCGGGGAAATATTTTGGATCATTCACCa        <  2:323211/100‑1 (MQ=255)
                                                                                             aaaTCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACg     <  2:2663627/100‑1 (MQ=255)
                                                                                              aaTCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACgn    <  2:904786/100‑2 (MQ=255)
                                                                                              aaTCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACgg    >  2:1374720/1‑100 (MQ=255)
                                                                                               aTCCCGGGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACggg   >  1:1277442/1‑100 (MQ=255)
                                                                                               aTCCC‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGga  <  1:1706821/100‑1 (MQ=255)
                                                                                                    |                                                                                                
CATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAAATCCC‑‑GGGGGGGATGATGCTCGTCCCGCTATTCCTTGGCGCACTGTGCCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGA  >  NC_000913/4101622‑4101816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: