Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,494,883 (A)7→8 coding (261/564 nt) idnK → D‑gluconate kinase, thermosensitive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,494,8761.A99.1% 429.2 / ‑3.3 114coding (254/564 nt)idnKD‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (56/57);  total (57/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 134 total aligned reads displayed.

ATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑AAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATG  >  NC_000913/4494778‑4494970
                                                                                                   |                                                                                               
aTCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑a                                                                                               <  1:1774666/100‑1 (MQ=255)
 tCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aa                                                                                              <  2:609688/100‑1 (MQ=255)
 tCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAACAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aa                                                                                              >  2:1727748/1‑100 (MQ=255)
  cTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaa                                                                                             >  1:880062/1‑100 (MQ=255)
    gatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaa                                                                                           <  2:214134/100‑1 (MQ=255)
    gatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaa                                                                                           >  2:314322/1‑100 (MQ=255)
      tgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaa                                                                                         >  1:844744/1‑100 (MQ=255)
        aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaac                                                                                       >  2:995846/1‑98 (MQ=255)
        aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaac                                                                                       <  1:520925/100‑3 (MQ=255)
        aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaac                                                                                       <  2:552853/100‑3 (MQ=255)
        aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaac                                                                                       >  1:644690/1‑98 (MQ=255)
          gATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaacag                                                                                     <  2:128080/100‑5 (MQ=255)
           aTCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaacagt                                                                                    >  1:1500199/1‑95 (MQ=255)
                   cccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGat                                                                             >  2:692748/1‑100 (MQ=255)
                   cccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGat                                                                             >  1:1202/1‑100 (MQ=255)
                   cccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGat                                                                             >  1:2627795/1‑100 (MQ=255)
                    ccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGata                                                                            >  2:1552560/1‑100 (MQ=255)
                         ctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTa                                                                       >  2:2772713/1‑100 (MQ=255)
                          tggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTAc                                                                      <  1:913820/100‑1 (MQ=255)
                           ggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACg                                                                     >  2:1496650/1‑100 (MQ=255)
                           ggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACg                                                                     <  1:2217711/100‑1 (MQ=255)
                           ggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACg                                                                     >  1:2174856/1‑100 (MQ=255)
                             aaCGCTTAAATGATGGTTCATACAGTCTTTATAAAAAGAAGGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGc                                                                    <  1:1685170/99‑1 (MQ=255)
                             aaCGCTTAAATAATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCAAAAAAAAAAAAAACAGTATCGTGATAttt                                                                         <  1:2403736/94‑1 (MQ=255)
                              aCGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCaa                                                                  <  1:543590/100‑1 (MQ=255)
                              aCGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCaa                                                                  <  2:1872575/100‑1 (MQ=255)
                               cGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAg                                                                 >  1:1343556/1‑100 (MQ=255)
                                gCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAgg                                                                >  1:1980899/1‑100 (MQ=255)
                                 cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAggg                                                               >  1:2848261/1‑100 (MQ=255)
                                 cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAggg                                                               <  1:341920/100‑1 (MQ=255)
                                 cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAggg                                                               >  1:322878/1‑100 (MQ=255)
                                   tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTa                                                             >  2:1356186/1‑100 (MQ=255)
                                   tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTa                                                             <  2:1107912/100‑1 (MQ=255)
                                    aaaTGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTa                                                             >  1:2745485/1‑100 (MQ=255)
                                       tgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGcc                                                          >  2:2296199/1‑99 (MQ=255)
                                        gatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGcccc                                                        <  1:760629/100‑1 (MQ=255)
                                        gatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGcccc                                                        <  2:950000/100‑1 (MQ=255)
                                         atgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGccccc                                                       >  2:2330580/1‑100 (MQ=255)
                                          tgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGccc                                                         >  1:1507048/1‑97 (MQ=255)
                                           gCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGccccc                                                       >  2:971027/1‑98 (MQ=255)
                                            cTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATg                                                    <  2:2051602/100‑1 (MQ=255)
                                                 tACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAt                                               >  2:923084/1‑100 (MQ=255)
                                                  aCAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAtt                                              <  2:2419028/100‑1 (MQ=255)
                                                  aCAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAtt                                              >  1:979340/1‑100 (MQ=255)
                                                    aGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGTGTAGCCCCCATGTTCATTTc                                            >  1:1004422/1‑100 (MQ=255)
                                                    aGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTc                                            >  2:2305330/1‑100 (MQ=255)
                                                     gTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTcc                                           <  2:1782964/100‑1 (MQ=255)
                                                       cTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctc                                         >  1:1540232/1‑100 (MQ=255)
                                                       cTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctc                                         >  2:2545028/1‑100 (MQ=255)
                                                       cTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctc                                         <  2:1540244/100‑1 (MQ=255)
                                                       cTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctc                                         >  2:1525104/1‑100 (MQ=255)
                                                        tttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctct                                        <  2:1974057/100‑1 (MQ=255)
                                                        tttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctct                                        >  1:396801/1‑100 (MQ=255)
                                                         ttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctct                                        <  2:2354342/100‑1 (MQ=255)
                                                          tataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTgg                                      <  2:61413/100‑1 (MQ=255)
                                                          tataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTgg                                      <  2:374974/100‑1 (MQ=255)
                                                          tataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTgg                                      >  1:321645/1‑100 (MQ=255)
                                                          tataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTgg                                      <  1:995842/100‑1 (MQ=255)
                                                           ataAAAAGAATGAAACTGGATTTTTTGTCTGTTAAACATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGt                                     <  1:1177189/100‑1 (MQ=255)
                                                           ataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTgg                                      >  1:1612898/1‑99 (MQ=255)
                                                           ataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGt                                     >  2:2626435/1‑100 (MQ=255)
                                                           ataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGt                                     <  2:1862464/100‑1 (MQ=255)
                                                            taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                    <  1:2545006/100‑1 (MQ=255)
                                                            taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                    <  1:2055797/100‑1 (MQ=255)
                                                            taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                    >  1:5921/1‑100 (MQ=255)
                                                             aaaaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTa                                   <  2:1277171/100‑1 (MQ=255)
                                                             aaaaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTa                                   <  2:2750029/100‑1 (MQ=255)
                                                              aaaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAg                                  <  1:1199662/100‑1 (MQ=255)
                                                              aaaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAg                                  >  2:1086217/1‑100 (MQ=255)
                                                              aaaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAg                                  >  2:1382897/1‑100 (MQ=255)
                                                                aaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGAt                                <  1:1496638/100‑1 (MQ=255)
                                                                   aaTGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGc                             >  1:1387818/1‑100 (MQ=255)
                                                                    aTGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCg                            <  1:1356179/100‑1 (MQ=255)
                                                                     tGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGcn                            <  2:1195266/99‑2 (MQ=255)
                                                                     tGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCg                            >  1:1611586/1‑99 (MQ=255)
                                                                       aaaCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGACCCCATGCTCATCAACTCTGGCAAGATgaaggtt                         >  2:527788/1‑94 (MQ=255)
                                                                        aaCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTa                        >  2:1138796/1‑100 (MQ=255)
                                                                         aCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTAt                       <  2:131334/100‑1 (MQ=255)
                                                                         aCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTAt                       >  2:2370745/1‑100 (MQ=255)
                                                                         aCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTAt                       <  2:396803/100‑1 (MQ=255)
                                                                            ggATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACt                         >  1:582162/1‑95 (MQ=255)
                                                                              aTTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                  <  2:506508/100‑1 (MQ=255)
                                                                              aTTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                  <  2:1966970/100‑1 (MQ=255)
                                                                              aTTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                  >  2:195808/1‑100 (MQ=255)
                                                                              aTTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                  <  1:2758112/100‑1 (MQ=255)
                                                                                 tATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTAt               <  1:1552548/100‑1 (MQ=255)
                                                                                 tATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGAAATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTAt               >  1:1552063/1‑100 (MQ=255)
                                                                                   ttGTCTGTTCATCATTAAAAAAAACAGTATCATGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtc             >  2:393960/1‑100 (MQ=255)
                                                                                    tGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtct            <  2:111970/100‑1 (MQ=255)
                                                                                     gTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtctc           >  1:100288/1‑100 (MQ=255)
                                                                                     gTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtctc           >  2:2273305/1‑100 (MQ=255)
                                                                                      tCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcg          <  2:13543/100‑1 (MQ=255)
                                                                                      tCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcg          <  2:1612910/100‑1 (MQ=255)
                                                                                       cTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgc         <  2:802391/100‑1 (MQ=255)
                                                                                        tGTTCATCATTAAAAAAAACAGTATCGTGATCTTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcg        >  2:1911525/1‑100 (MQ=255)
                                                                                         gTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgc       >  2:2254241/1‑100 (MQ=255)
                                                                                          ttCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgcg      <  2:1458817/100‑1 (MQ=255)
                                                                                             atcatTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGaa    <  1:225223/99‑1 (MQ=255)
                                                                                              tcatTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATg  <  2:1507060/100‑1 (MQ=255)
                                                                                              tcatTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATg  >  1:1119895/1‑100 (MQ=255)
                                                                                                   |                                                                                               
ATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑AAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATG  >  NC_000913/4494778‑4494970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: