Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,1520AG23.8% 188.9 / 25.7 101intergenic (+135/‑63)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (58/19);  new base G (1/23);  total (59/42)
Fisher's exact test for biased strand distribution p-value = 2.55e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

GGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCG  >  NC_000913/3781053‑3781250
                                                                                                       |                                                                                                   
gggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGa                                                                                                     >  1:199457/1‑100 (MQ=255)
 ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAg                                                                                                    <  2:2284177/100‑1 (MQ=255)
 ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAg                                                                                                    <  1:2855493/100‑1 (MQ=255)
   agagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAggg                                                                                                  <  2:204659/100‑1 (MQ=255)
     agGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑a                                                                                               <  1:1092790/100‑1 (MQ=255)
      ggggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑aa                                                                                              >  1:418643/1‑100 (MQ=255)
      ggggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑‑TTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑aaa                                                                                             >  2:174712/1‑100 (MQ=255)
           ccGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGc                                                                                         >  2:397618/1‑100 (MQ=255)
             gTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGC‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCg                                                                                       >  2:1751817/1‑100 (MQ=255)
             gTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCg                                                                                       >  1:1703625/1‑100 (MQ=255)
                 ggCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGG‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCAc                                                                                    >  2:577698/1‑99 (MQ=255)
                  gcgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTg                                                                                  >  2:1177882/1‑100 (MQ=255)
                     cTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTc                                                                               >  1:2287254/1‑100 (MQ=255)
                       gtatgtaCTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTTTTTTTTT‑TCTCTTCGGGGA‑GAGGGCCGGGG‑TGAGGTG‑AAAAGGCCGCACTGCTCAc                                                                             >  1:1849075/1‑100 (MQ=255)
                       gtatgtaCTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCAc                                                                             >  2:2746037/1‑100 (MQ=255)
                       gtatgtaCTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCAc                                                                             >  2:1293058/1‑100 (MQ=255)
                            taCTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAAtt                                                                        >  1:2178075/1‑100 (MQ=255)
                            taCTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAAtt                                                                        >  2:2655633/1‑100 (MQ=255)
                             aCTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAAttt                                                                       >  1:1877732/1‑100 (MQ=255)
                              ccccccccctcgGAAACGCCCCCCCCCTT‑TTTTTTTCTCCC‑CC‑CCCCCCCGGGGAGGGGGGCGGGGG‑TGGGGGG‑AAAAGGCCGCACTGCTCACCAAttt                                                                       <  2:1476132/91‑1 (MQ=255)
                              cTCCCTCACCCTGAAACGACACCGCACTC‑TTTTTTTCGCCCCCG‑CCCCCCGGGGGG‑GGGGGCGGGGG‑TGGGGGG‑AAAAGGCCGCACTGCTCACCAAttt                                                                       <  1:788495/99‑1 (MQ=255)
                                cccTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCt                                                                    >  1:1695912/1‑100 (MQ=255)
                                 cttccctgtGAAACGCCCCCGCTCTT‑TTTTTTTCCCCCCCG‑CCCCGCGGGGGA‑GGGGGCGGGGG‑GGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTg                                                                   <  2:2779575/92‑1 (MQ=255)
                                 cctccctcGAAACCGACACCGCACTT‑TTTTTTTCCCCCCCC‑CCCCCCCGGGGA‑GAGGCCCGGGG‑TGGGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTg                                                                   <  1:359976/100‑1 (MQ=255)
                                 ccctcccccgAAACGCCCCCGC‑CTCTTTTTTTTCCCCCCCGCCCCCACCGGGGC‑GAGGCCCGGGG‑GGGGGGG‑AAAAGGCCGCACTGCTCACCAAttt                                                                       <  1:2209872/88‑1 (MQ=255)
                                 ccccaccaggAAACGCCCCCCCTCTT‑TTTTTTTCCCCCCCG‑CCCCCCGGGGGG‑GAGGCCGGGGG‑GGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTg                                                                   <  2:418645/91‑1 (MQ=255)
                                 ccTCACTCTGAAACGACACCGCACTC‑TTTTTTTTTTTCTTT‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTg                                                                   >  1:1337461/1‑100 (MQ=255)
                                 ccTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTg                                                                   >  1:287401/1‑100 (MQ=255)
                                  ccccccctGAAACGACACCGCCCTC‑TTTTTTTCCCCCCCG‑CCCCCCCGGGGA‑GAGGGCGGGGG‑TGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                  <  1:1382967/94‑1 (MQ=255)
                                  cTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                  >  2:827829/1‑100 (MQ=255)
                                    cACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTa                                                                >  2:1010341/1‑100 (MQ=255)
                                      gtgggagaCGACCCCGCGGTG‑TTTTTTTCCCCCTCG‑CCCCTCCGGGGA‑G‑GGGCCGGGG‑GGGGGGGAAAAAGGCCGCACTGCTCACCAATTTTCt                                                                    <  2:924364/87‑1 (MQ=19)
                                      ctctGAGACGACCCCGCACTT‑TTTTTTTCTCCCTCG‑CCCCCCGGGGGA‑GGGGGCGGGGT‑GGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                  <  1:632796/96‑1 (MQ=255)
                                      ctctGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATc                                                              >  1:2423273/1‑100 (MQ=255)
                                      ctctGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATc                                                              >  1:2357967/1‑100 (MQ=255)
                                        ctGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                            >  1:2771376/1‑100 (MQ=255)
                                        ctGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑CGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                            >  2:478960/1‑100 (MQ=255)
                                          gAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGc                                                          >  1:1494663/1‑100 (MQ=255)
                                          gAAACGACACCACACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑GGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGc                                                          >  2:129613/1‑100 (MQ=255)
                                            aaCGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc                                                        >  2:2210166/1‑100 (MQ=255)
                                             aCGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                                       >  2:354872/1‑100 (MQ=255)
                                              caccacCCCCCTC‑TTTTTTTCCCCCCCC‑CCCCT‑CGGGGG‑GGGGGCCGGGGTTGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                                       <  1:462177/96‑1 (MQ=255)
                                              cGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                                      >  1:1451203/1‑100 (MQ=255)
                                              cGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                                      >  1:2455788/1‑100 (MQ=255)
                                              cGACACCACACTC‑TTTTTTTATCCCTCG‑CCCCTCCGGGGA‑GCGGGACGGTG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                                      >  1:2555978/1‑100 (MQ=38)
                                                 ccccGCACTT‑TTTTTTCCCCCCCCC‑CCCCCCGGGGGA‑GGGGGCGGGGG‑GGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                                     <  2:2287272/96‑1 (MQ=255)
                                                   ccGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGT‑TGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCa                                                   <  1:1159782/98‑1 (MQ=255)
                                                    ccccctc‑tTTTTTCCCCCCTCC‑CCCCCCGGGGGA‑GGGGGCCGGGG‑TGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa                                                <  2:385406/96‑1 (MQ=255)
                                                     gccttc‑tTTTTTTCCCCCCCC‑CCCCCCGGGGGG‑GGGGCCCGGGG‑GGGGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCAc                                                  <  1:5026/93‑1 (MQ=255)
                                                          c‑tTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                          >  1:1725801/1‑100 (MQ=255)
                                                          c‑tTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                          >  2:1207358/1‑100 (MQ=255)
                                                          c‑tTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAAATTTATGCTATCCTGCCCCCCCACTACgggg                                           >  1:1133388/1‑99 (MQ=38)
                                                           tttttttcCCCCCCCG‑CCCCTCGGGGGG‑GGGGGCGGGGG‑TGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                          <  2:199458/99‑1 (MQ=255)
                                                            tttttttCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                         >  1:2360067/1‑100 (MQ=255)
                                                            ttttttcCCCCCGCG‑CCCCGCGGGGGG‑GGGGGGGGGGG‑GGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                          <  2:1313101/99‑1 (MQ=255)
                                                            ttttttcCCCCCCCG‑CCCCCCGGGGGG‑GGGGGCGGGGG‑TGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg                                            <  1:2884165/97‑1 (MQ=255)
                                                                tttCCCCCCCC‑CCCCCCGGGGGA‑GAGGGCCGGGG‑GGAGGGG‑AAAAGGCCGCACTGCTTACCAATTTTCTGCTATCCTGCCCCCGCATTAAGGGGGCAGTa                                     <  2:1948566/100‑1 (MQ=255)
                                                                ttcccccccCG‑CCCCCCGGGGGG‑GGGGGCCGGGG‑GGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa                                     <  2:1703637/95‑1 (MQ=255)
                                                                tgccccccTCC‑CCCCCCGGGGGA‑G‑GGGCCGGGG‑TGAGGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAg                                       <  2:2161046/93‑1 (MQ=255)
                                                                  tctcCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATg                                   >  1:320094/1‑100 (MQ=255)
                                                                   tccccTCC‑CCCCGCGGGGGG‑GGGGGCGGGGG‑GGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                                  <  2:2118510/98‑1 (MQ=255)
                                                                     cccccccccccccGGGGGA‑GGGGGCCGGGG‑GGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt                                 <  2:1746197/93‑1 (MQ=255)
                                                                     cccTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                                >  2:424040/1‑100 (MQ=255)
                                                                          g‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACAt                           >  2:924352/1‑100 (MQ=255)
                                                                            ccccccGGGGGG‑GGGGCCCGGGG‑TGAGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                          <  2:1695924/100‑1 (MQ=255)
                                                                            ccccTCGGGGGG‑GGGGGCGGGGG‑TGAGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACAt                           <  1:827826/99‑1 (MQ=255)
                                                                            ccccTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                          >  2:1062840/1‑100 (MQ=255)
                                                                            ccccTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                          >  2:2499280/1‑100 (MQ=255)
                                                                             cccgcgggggA‑GGGGGCGGGGG‑GGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                         <  1:2865667/94‑1 (MQ=255)
                                                                              ccTCGGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt                        <  1:2775977/100‑1 (MQ=255)
                                                                              ccTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt                        >  2:1602749/1‑100 (MQ=255)
                                                                              ccTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt                        >  2:1602241/1‑100 (MQ=255)
                                                                               cTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                       >  2:1508993/1‑100 (MQ=255)
                                                                               cTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                       >  1:1543111/1‑100 (MQ=255)
                                                                                 cgggggg‑gggggCCGGGG‑GGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc                      <  2:2423294/97‑1 (MQ=255)
                                                                                 ccGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt                     >  1:953095/1‑100 (MQ=255)
                                                                                  gggggg‑gggggCGGGGG‑GGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                       <  2:1441500/96‑1 (MQ=255)
                                                                                   ggggg‑gTGGGCCGGGG‑TGAGGGG‑ACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt                   <  2:287402/100‑1 (MQ=255)
                                                                                   ggggA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt                   <  2:953098/100‑1 (MQ=255)
                                                                                   ggggA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt                   >  2:1508377/1‑100 (MQ=255)
                                                                                   ggggA‑GAGGCCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc                      <  1:2746012/97‑1 (MQ=255)
                                                                                    gggA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTa                  >  2:2209441/1‑100 (MQ=255)
                                                                                     ggA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                 >  2:2042506/1‑100 (MQ=255)
                                                                                      ggggggggCGGGGG‑GGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                 <  2:1489305/95‑1 (MQ=255)
                                                                                      ggggggggCCGGGG‑TGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                 <  1:966315/95‑1 (MQ=255)
                                                                                      ga‑ggGGGCGGGGG‑GGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                       <  1:5475/93‑1 (MQ=255)
                                                                                         gagGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGaa              >  2:1902809/1‑100 (MQ=255)
                                                                                          agGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAc             >  2:1070315/1‑100 (MQ=255)
                                                                                           gggCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa               <  1:308883/97‑1 (MQ=255)
                                                                                           gggCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc            >  2:1369934/1‑100 (MQ=255)
                                                                                           gggCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc            >  1:2196150/1‑100 (MQ=255)
                                                                                           gggCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAc             >  1:561702/1‑99 (MQ=255)
                                                                                            ggCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa           >  1:79143/1‑100 (MQ=255)
                                                                                             gCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg          >  2:1397457/1‑100 (MQ=255)
                                                                                              ccGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa         >  1:1562759/1‑100 (MQ=255)
                                                                                              ccGGGG‑GGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa         <  2:1342941/100‑1 (MQ=255)
                                                                                                gggg‑TGAGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaaa       <  1:577696/100‑1 (MQ=255)
                                                                                                  gg‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAAtt     >  2:2676321/1‑100 (MQ=255)
                                                                                                   g‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTc    >  1:294002/1‑100 (MQ=255)
                                                                                                      gAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCg  <  2:294003/100‑1 (MQ=255)
                                                                                                       |                                                                                                   
GGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTTCTCCCTCG‑CCCCTCCGGGGA‑GAGGGCCGGGG‑TGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCG  >  NC_000913/3781053‑3781250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑