Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,541,831 C→T G254R (GGG→AGG)  narZ ← nitrate reductase 2 (NRZ), alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,541,8310CT97.7% 301.1 / ‑2.1 87G254R (GGG→AGG) narZnitrate reductase 2 (NRZ), alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (43/42);  total (43/44)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCG  >  NC_000913/1541736‑1541925
                                                                                               |                                                                                              
gtagtCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAg                                                                                            <  1:2452088/100‑1 (MQ=255)
  agtCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGc                                                                                          >  2:2864759/1‑100 (MQ=255)
  agtCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGc                                                                                          <  2:2505357/100‑1 (MQ=255)
   gtCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCg                                                                                         >  1:2761466/1‑100 (MQ=255)
   gtCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTAAGTTAGACCTCCAGGCg                                                                                         >  1:1688764/1‑100 (MQ=255)
     cAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCgat                                                                                       >  1:843795/1‑100 (MQ=255)
     cAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCgat                                                                                       <  2:970333/100‑1 (MQ=255)
     cAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCgat                                                                                       <  1:1699149/100‑1 (MQ=255)
       ggggTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCgatga                                                                                     <  2:2482766/100‑1 (MQ=255)
       ggggTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCgatga                                                                                     >  2:2347401/1‑100 (MQ=255)
       ggggTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCgatga                                                                                     >  2:2184533/1‑100 (MQ=255)
       ggggTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCgatga                                                                                     >  1:2648342/1‑100 (MQ=255)
        gggTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCgatgat                                                                                    <  1:2560966/100‑1 (MQ=255)
          gTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGt                                                                                  <  1:1656924/100‑1 (MQ=255)
          gTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGt                                                                                  >  1:1200888/1‑100 (MQ=255)
          gTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGt                                                                                  >  2:876184/1‑100 (MQ=255)
          gTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGt                                                                                  <  1:1815183/100‑1 (MQ=255)
           tAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTa                                                                                 <  2:1223923/100‑1 (MQ=255)
            aaTGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTAGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAg                                                                                >  1:675916/1‑100 (MQ=255)
               ggCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTg                                                                             >  1:2058484/1‑100 (MQ=255)
                gCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTgg                                                                            >  1:2078404/1‑100 (MQ=255)
                  gATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAg                                                                          <  2:80838/100‑1 (MQ=255)
                   aTGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGt                                                                         <  2:2248815/100‑1 (MQ=255)
                   aTGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGt                                                                         <  1:2184515/100‑1 (MQ=255)
                   aTGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGt                                                                         <  1:1939417/100‑1 (MQ=255)
                   aTGGTTTTAGTGCCTTCGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGt                                                                         <  1:1689698/100‑1 (MQ=255)
                    tGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGtt                                                                        <  1:2768035/100‑1 (MQ=255)
                      gTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTtat                                                                      <  1:1225892/100‑1 (MQ=255)
                       ttttAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTtata                                                                     <  1:2669390/100‑1 (MQ=255)
                        tttAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATAc                                                                    <  2:504963/100‑1 (MQ=255)
                         ttAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATAcc                                                                   <  1:2307357/100‑1 (MQ=255)
                          tAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCa                                                                  >  2:2522413/1‑100 (MQ=255)
                          tAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCa                                                                  >  1:510655/1‑100 (MQ=255)
                           aGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAg                                                                 <  1:478828/100‑1 (MQ=255)
                           aGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAg                                                                 <  1:1463801/100‑1 (MQ=255)
                            gTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGt                                                                >  1:1082969/1‑100 (MQ=255)
                              gCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGGTATACCAGTCg                                                              >  2:2702210/1‑100 (MQ=255)
                               ccTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCgg                                                             >  2:2099060/1‑100 (MQ=255)
                                cTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGc                                                            >  2:2294238/1‑100 (MQ=255)
                                cTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGc                                                            >  2:2294014/1‑100 (MQ=255)
                                 tttGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCa                                                           >  2:2196294/1‑100 (MQ=255)
                                 tttGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCa                                                           >  2:2783339/1‑100 (MQ=255)
                                 tttGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCa                                                           <  1:88742/100‑1 (MQ=255)
                                  ttGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAg                                                          <  1:62546/100‑1 (MQ=255)
                                      aGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTc                                                      >  2:2770697/1‑100 (MQ=255)
                                       gCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTcc                                                     <  2:2403706/100‑1 (MQ=255)
                                       gCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTcc                                                     >  1:943143/1‑100 (MQ=255)
                                        cGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCg                                                    <  2:1082974/100‑1 (MQ=255)
                                          tACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGt                                                  <  1:2522391/100‑1 (MQ=255)
                                            cTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTAc                                                >  1:2465419/1‑100 (MQ=255)
                                            cTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTAc                                                >  1:2491896/1‑100 (MQ=255)
                                             ttCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACg                                               >  2:2518317/1‑100 (MQ=255)
                                             ttCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACg                                               <  2:2761491/100‑1 (MQ=255)
                                                ggTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGGGTTATACCAGTCGGCAGATTCCGGTACGTCg                                            >  2:1246782/1‑100 (MQ=255)
                                                ggTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCg                                            >  1:930132/1‑100 (MQ=255)
                                                ggTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCg                                            >  1:926833/1‑100 (MQ=255)
                                                ggTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCg                                            <  2:2078422/100‑1 (MQ=255)
                                                 gTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCgg                                           >  1:599557/1‑100 (MQ=255)
                                                 gTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCgg                                           >  1:2849915/1‑100 (MQ=255)
                                                    aagaagTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGttt                                        >  1:1967212/1‑100 (MQ=255)
                                                     agaagTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTg                                       <  1:2190020/100‑1 (MQ=255)
                                                      gaagTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGc                                      >  2:50821/1‑100 (MQ=255)
                                                       aagTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCt                                     <  2:1200893/100‑1 (MQ=255)
                                                        aatGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTc                                    >  2:1956144/3‑100 (MQ=255)
                                                         gTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCg                                   <  1:412887/100‑1 (MQ=255)
                                                           gggCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGcc                                 <  1:540303/100‑1 (MQ=255)
                                                           gggCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGcc                                 >  2:1477065/1‑100 (MQ=255)
                                                                tCCGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAgg                            <  2:1398759/100‑1 (MQ=255)
                                                                 ccGGCGTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAgg                            >  2:295136/1‑99 (MQ=255)
                                                                      gTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCg                      >  1:830787/1‑100 (MQ=255)
                                                                      gTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCg                      >  1:707851/1‑100 (MQ=255)
                                                                      gTACGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCg                      >  1:687393/1‑100 (MQ=255)
                                                                         cGTGTCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCg                   <  1:1988256/100‑1 (MQ=255)
                                                                            gtCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAgg                >  1:967280/1‑100 (MQ=255)
                                                                            gtCTGCGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAgg                <  1:2770672/100‑1 (MQ=255)
                                                                                 cGGTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAggcgggc           <  2:675918/100‑1 (MQ=255)
                                                                                  ggTACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAggcgggcg          >  1:2144890/1‑100 (MQ=255)
                                                                                    ttcGTTAGACCTCAAGTCGATGTTGTAGCTGGAGCTATACCAGTCGGCAGATTCAGGTACGTCGGTTTGCTCGCCTCAGGTCATCGGCTAcgcgggcg          <  1:1297624/96‑1 (MQ=255)
                                                                                    tACGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGggcggc        >  2:1625575/1‑100 (MQ=255)
                                                                                     aCGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCa       <  2:707853/100‑1 (MQ=255)
                                                                                      cGTTAGCCCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCa       <  1:876181/99‑1 (MQ=255)
                                                                                      cGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAg      <  2:961520/100‑1 (MQ=255)
                                                                                      cGTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAg      <  1:2099042/100‑1 (MQ=255)
                                                                                       gTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAgg     <  1:2864733/100‑1 (MQ=255)
                                                                                       gTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAgg     >  2:626123/1‑100 (MQ=255)
                                                                                       gTTAGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAgg     <  1:1477053/100‑1 (MQ=255)
                                                                                          aGACCTCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCg  <  2:830790/100‑1 (MQ=255)
                                                                                               |                                                                                              
GTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCG  >  NC_000913/1541736‑1541925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑