Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,252,176 T→C intergenic (+166/‑128) yqjG → / → yhaH putative S‑transferase/DUF805 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,252,1760TC97.5% 420.1 / ‑0.7 119intergenic (+166/‑128)yqjG/yhaHputative S‑transferase/DUF805 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (53/63);  total (55/64)
Fisher's exact test for biased strand distribution p-value = 5.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 124 total aligned reads displayed.

GAACGCCTTATCTGACATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGTCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCTTAATTTGCTGCTT  >  NC_000913/3252079‑3252269
                                                                                                 |                                                                                             
gAACGCCTTATCTGACATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGcca                                                                                             <  2:1899625/100‑1 (MQ=255)
  aCGCCTTATCTGACATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGccatc                                                                                           >  1:2666065/1‑100 (MQ=255)
  aCGCCTTATCTGACATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGccatc                                                                                           <  2:1315288/100‑1 (MQ=255)
     ccTTATCTGACATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGt                                                                                        >  2:2686230/1‑100 (MQ=255)
     ccTTATCTGACATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGt                                                                                        >  1:1586404/1‑100 (MQ=255)
      cTTATCTGACATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTc                                                                                       <  2:799125/100‑1 (MQ=255)
       ttATCTGACATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCt                                                                                      >  1:453977/1‑100 (MQ=255)
       ttATCTGACATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCt                                                                                      <  2:1191721/100‑1 (MQ=255)
            tGACATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAgcg                                                                                 <  2:920857/100‑1 (MQ=255)
            tGACATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAgcg                                                                                 >  2:2860456/1‑100 (MQ=255)
             gACATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAgcgn                                                                                <  1:313430/100‑2 (MQ=255)
               cATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAgcgcgc                                                                              >  2:1289902/1‑100 (MQ=255)
               cATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAgcgcgc                                                                              >  1:623994/1‑100 (MQ=255)
                aTACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGcc                                                                             <  1:1971494/100‑1 (MQ=255)
                 tACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCt                                                                            >  2:1511359/1‑100 (MQ=255)
                 tACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCt                                                                            <  2:359322/100‑1 (MQ=255)
                 tACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCt                                                                            <  1:893872/100‑1 (MQ=255)
                  aCAAAACATTGTACATTCAATAAATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCtt                                                                           >  1:1677104/1‑100 (MQ=255)
                   cAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTc                                                                          >  2:1557060/1‑100 (MQ=255)
                     aaaCATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCAc                                                                        <  2:2375610/100‑1 (MQ=255)
                      aaCATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACg                                                                       <  2:2720272/100‑1 (MQ=255)
                       aCATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACgg                                                                      <  1:2572343/100‑1 (MQ=255)
                       aCATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCAGCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACgg                                                                      <  1:2292134/100‑1 (MQ=255)
                        cATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGc                                                                     <  2:445225/100‑1 (MQ=255)
                        cATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGc                                                                     <  2:64366/100‑1 (MQ=255)
                        cATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGc                                                                     >  2:503368/1‑100 (MQ=255)
                        cATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGc                                                                     <  1:555731/100‑1 (MQ=255)
                          ttGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGTCATCAGTCTTAGCGCGCCTTCACGGCAc                                                                   >  2:2154472/1‑100 (MQ=255)
                           tGTACATTCAATAGATTGCATAACCCGCGCAGACCAGCTAAGCGTAGCGCATCTGGCGATTTTGCGCTTGTCATCAGTCTTAGCGCGCCTTCACGGCAc                                                                   >  2:2873491/1‑99 (MQ=255)
                           tGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACt                                                                  >  2:1342146/1‑100 (MQ=255)
                            gTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACtt                                                                 >  1:711324/1‑100 (MQ=255)
                            gTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATATAGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACtt                                                                 >  1:706094/1‑100 (MQ=255)
                             tACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACttt                                                                >  1:906670/1‑100 (MQ=255)
                              aCATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTtta                                                               <  1:1384588/100‑1 (MQ=255)
                              aCATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTtta                                                               >  1:2357294/1‑100 (MQ=255)
                                aTTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTttatt                                                             <  1:2644025/100‑1 (MQ=255)
                                 ttCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTttatta                                                            >  1:2252020/1‑100 (MQ=255)
                                   cAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAAt                                                          >  2:1691411/1‑100 (MQ=255)
                                   cAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAAt                                                          >  1:887619/1‑100 (MQ=255)
                                     aTAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAAtt                                                         >  2:1960927/1‑99 (MQ=255)
                                      tAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTcc                                                       <  2:1154642/100‑1 (MQ=255)
                                       aGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCt                                                      <  2:356211/100‑1 (MQ=255)
                                       aGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCt                                                      <  1:1511347/100‑1 (MQ=255)
                                        gATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCtt                                                     >  2:1942536/1‑100 (MQ=255)
                                        gATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCtt                                                     <  1:308622/100‑1 (MQ=255)
                                        gATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCtt                                                     >  1:2383829/1‑100 (MQ=255)
                                         aTTGCATGACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCATTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTg                                                    <  1:1551079/100‑1 (MQ=255)
                                         aTTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTg                                                    >  2:2228596/1‑100 (MQ=255)
                                         aTTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTg                                                    <  1:2480272/100‑1 (MQ=255)
                                            gCATAACCCGCGGAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGATTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGaa                                                  >  1:2445301/1‑99 (MQ=255)
                                            gCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAAc                                                 >  1:2154066/1‑100 (MQ=255)
                                            gCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAAc                                                 >  1:208405/1‑100 (MQ=255)
                                            gCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAAc                                                 <  2:896553/100‑1 (MQ=255)
                                            gCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAAc                                                 <  2:1677116/100‑1 (MQ=255)
                                            gCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAAc                                                 >  2:568061/1‑100 (MQ=255)
                                            gCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCCTCACGGCACTTTATTAATTCCTTGAAc                                                 >  1:986941/1‑100 (MQ=255)
                                               tAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGaa                                              >  1:1654258/1‑100 (MQ=255)
                                                aaCCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAat                                             <  1:1691399/100‑1 (MQ=255)
                                                aaCCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAat                                             <  2:593354/100‑1 (MQ=255)
                                                aaCCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAat                                             <  1:2686206/100‑1 (MQ=255)
                                                 aCCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAata                                            >  1:2246747/1‑100 (MQ=255)
                                                 aCCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAata                                            >  1:2832792/1‑100 (MQ=255)
                                                  cccGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAatat                                           <  1:1262519/100‑1 (MQ=255)
                                                   ccGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATAtt                                          <  2:1654270/100‑1 (MQ=255)
                                                      cgcAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGTCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTAc                                       <  1:2154454/100‑1 (MQ=255)
                                                        cAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTg                                     <  2:2251844/100‑1 (MQ=255)
                                                         aGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGc                                    >  2:395667/1‑100 (MQ=255)
                                                          gACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGcc                                   <  2:1596148/100‑1 (MQ=255)
                                                          gACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGcc                                   >  2:1397068/1‑100 (MQ=255)
                                                          gACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGcc                                   >  1:1959944/1‑100 (MQ=255)
                                                           aCCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAGTATTTACTGCCa                                  >  2:2615225/1‑100 (MQ=255)
                                                            ccAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCAt                                 >  2:1604797/1‑100 (MQ=255)
                                                            ccAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCAt                                 >  1:1937948/1‑100 (MQ=255)
                                                              aGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAGTTCCTTGAACGAATATTTACTGCCAttt                               >  2:1763615/1‑100 (MQ=255)
                                                               gATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATttc                              <  2:2530430/100‑1 (MQ=255)
                                                                 tAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATttcat                            <  2:906673/100‑1 (MQ=255)
                                                                 tAAGCGTAGCGCATCTGGCGATTTTGCGCTTGCCACCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATttcat                            <  2:1150219/100‑1 (MQ=255)
                                                                  aaGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATttcatt                           <  2:2252038/100‑1 (MQ=255)
                                                                   aGCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATttcattc                          >  1:832299/1‑100 (MQ=255)
                                                                    gCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATttcattca                         >  1:1025548/1‑100 (MQ=255)
                                                                    gCGTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATttcattca                         >  2:1558406/1‑100 (MQ=255)
                                                                      gTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACg                       <  1:503366/100‑1 (MQ=255)
                                                                      gTAGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACg                       <  2:2357312/100‑1 (MQ=255)
                                                                        aGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTc                     <  2:865269/100‑1 (MQ=255)
                                                                        aGCGCATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTc                     <  2:2262945/100‑1 (MQ=255)
                                                                            cATCTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTAtt                 <  1:1716414/100‑1 (MQ=255)
                                                                               cTGGCGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCtt              <  1:2014592/100‑1 (MQ=255)
                                                                                tGGCGATTTTGCGCTTGCCATCATTCTTAACGCGCCTTCACGGCACTTTACTACTTCCTTGACCGGATATTTACTGCCATTTCATTCACGTCTATTCtt              >  1:412124/1‑99 (MQ=255)
                                                                                   cGATTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCTTAAtt          <  1:2228578/100‑1 (MQ=255)
                                                                                     aTTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCTTAATTtg        >  1:2047651/1‑100 (MQ=255)
                                                                                     aTTTTGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCTTAATTtg        <  1:129290/100‑1 (MQ=255)
                                                                                      ttttGCGCTTGCCATCAGTCTTAGCGCGCCTTCCCGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCTTAATTtgc       <  2:115919/100‑1 (MQ=255)
                                                                                      ttttGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCTTAATTtgc       <  2:2663988/100‑1 (MQ=255)
                                                                                      ttttGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCTTAATTtgc       <  2:621986/100‑1 (MQ=255)
                                                                                      ttttGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCTTAATTtgc       <  2:754231/100‑1 (MQ=255)
                                                                                       tttGCGCTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCTTAATTtgct      <  1:1289895/100‑1 (MQ=255)
                                                                                          gcgcTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCTTAATTTgctgct   >  1:1314067/1‑100 (MQ=255)
                                                                                          gcgcTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCTTAATTTgctgct   <  1:2498308/100‑1 (MQ=255)
                                                                                           cgcTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCTTAATTTGCTGCtt  <  1:568059/100‑1 (MQ=255)
                                                                                           cgcTTGCCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCTTAATTTGCTGCtt  >  2:832874/1‑100 (MQ=255)
                                                                                                 |                                                                                             
GAACGCCTTATCTGACATACAAAACATTGTACATTCAATAGATTGCATAACCCGCGCAGACCAGATAAGCGTAGCGCATCTGGCGATTTTGCGCTTGTCATCAGTCTTAGCGCGCCTTCACGGCACTTTATTAATTCCTTGAACGAATATTTACTGCCATTTCATTCACGTCTATTCTTAATTTGCTGCTT  >  NC_000913/3252079‑3252269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑