Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,512,561 Δ1 bp 34.0% intergenic (‑217/+73) yhfZ ← / ← trpS putative DNA‑binding transcriptional regulator/tryptophanyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,512,5600T.34.0% 365.5 / 198.8 150intergenic (‑216/+74)yhfZ/trpSputative DNA‑binding transcriptional regulator/tryptophanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (52/47);  new base . (27/24);  total (79/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01

Only 100 of 157 total aligned reads displayed.

TAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCAATCG  >  NC_000913/3512462‑3512659
                                                                                                  |                                                                                                   
tAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtt                                                                                                    >  2:3135173/1‑100 (MQ=255)
tAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAta                                                                                                    >  2:2160203/1‑99 (MQ=255)
tAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAta                                                                                                    >  1:684701/1‑99 (MQ=255)
 aaaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtac                                                                                                   >  1:1703781/1‑98 (MQ=255)
  aaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtact                                                                                                  >  1:2297730/1‑97 (MQ=255)
  aaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtact                                                                                                  >  2:1025461/1‑97 (MQ=255)
  aaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTAc                                                                                                  <  2:2010312/100‑1 (MQ=255)
  aaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTAc                                                                                                  >  1:50993/1‑100 (MQ=255)
    aaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACtt                                                                                                >  2:1928272/1‑100 (MQ=255)
      aaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTg                                                                                              <  1:2999862/100‑1 (MQ=255)
      aaCCGAGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTg                                                                                              <  1:2770860/100‑1 (MQ=255)
        ccGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTa                                                                                            <  1:962770/100‑1 (MQ=255)
        ccGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTa                                                                                            >  1:2889826/1‑100 (MQ=255)
        ccGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTa                                                                                            >  2:193437/1‑100 (MQ=255)
        ccGGGTTTCCCCGGTTGCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTa                                                                                            <  1:724762/100‑1 (MQ=255)
         cGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAg                                                                                           >  1:1081319/1‑100 (MQ=255)
         cGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAg                                                                                           >  2:418996/1‑100 (MQ=255)
           gggaaacccGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGcc                                                                                        >  2:892954/7‑100 (MQ=37)
             tttCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCg                                                                                       <  1:1897134/100‑1 (MQ=255)
               tCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGa                                                                                     <  2:2241717/100‑1 (MQ=255)
                ccccGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGAt                                                                                    >  2:274163/1‑100 (MQ=255)
                ccccGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGAt                                                                                    >  2:41665/1‑100 (MQ=255)
                 cccGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATa                                                                                   >  2:790578/1‑100 (MQ=255)
                    ggTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAgg                                                                                <  1:1541321/100‑1 (MQ=255)
                    ggTTGCAGCGTGCTGACAAGAGCAAAATTGCCTGATTCGCTACGCATATCAGTCCTACATTTCCTAGCAATATGTGCATTACTTTGTATGCCTGATAAgg                                                                                <  1:3089221/100‑1 (MQ=21)
                       ttCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGt                                                                             >  1:162075/1‑100 (MQ=255)
                        tCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGTCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTc                                                                           <  1:362547/100‑1 (MQ=255)
                        tCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTc                                                                           >  2:625220/1‑100 (MQ=255)
                         cAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCa                                                                          <  2:2855977/100‑1 (MQ=255)
                            agTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCAcgc                                                                       <  1:2232443/100‑1 (MQ=255)
                             gTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCAcgc                                                                       <  2:50993/100‑1 (MQ=255)
                             gTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCAcgcc                                                                      >  2:1035452/1‑100 (MQ=255)
                              tgatgaTAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCAcgccg                                                                     <  2:491930/100‑1 (MQ=255)
                               gatgatAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCAcgccgc                                                                    >  1:2510675/1‑100 (MQ=255)
                               gatgatAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCAcgccgc                                                                    >  1:2796595/1‑100 (MQ=255)
                               gatgatAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGGGCATTACTTTGTAGGCCGGATAAGGCGTTCAcgcc                                                                      >  2:2423696/1‑99 (MQ=255)
                                 tgatAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCa                                                                   <  2:3341088/100‑1 (MQ=255)
                                  gatAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                  >  2:3315800/1‑100 (MQ=255)
                                  gatAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                  >  2:1445649/1‑100 (MQ=255)
                                  gatAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                  >  1:717521/1‑100 (MQ=255)
                                   atAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATGCGGCGTGAAcgccttatc                                                                 <  2:2209620/100‑6 (MQ=25)
                                   atAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAAGCCTACATTTCCTTGCAATATGTGTATACTCTATATTCCGTTTAAGGCGTTCCCTCCTCAt                                                                  >  1:2156070/1‑98 (MQ=12)
                                    tAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                >  2:270682/1‑100 (MQ=255)
                                    tAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                               <  2:1172306/100‑1 (MQ=255)
                                        gatctaaTTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                            <  1:1913501/95‑1 (MQ=255)
                                        aGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                                                           >  2:1136884/1‑100 (MQ=255)
                                        aGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                                                           <  1:260689/100‑1 (MQ=25)
                                         gCAAAATTGCCTGCTGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTCCTTTGTCGGCCGGATAAGGCGTTCACGCCCCATCCgg                                                              >  2:2896245/1‑97 (MQ=37)
                                         gCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATg                                                          <  2:2729038/100‑1 (MQ=255)
                                          cAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATg                                                          >  2:3439123/1‑100 (MQ=255)
                                             aaTTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAc                                                       <  1:829200/100‑1 (MQ=255)
                                             aaTTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAc                                                       <  2:1997468/100‑1 (MQ=255)
                                             aaTTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGatc                                                       >  2:1913517/1‑98 (MQ=255)
                                                    tGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAgcgn                                                <  1:565743/100‑2 (MQ=255)
                                                    tGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCa                                               >  2:2800305/1‑100 (MQ=255)
                                                     gATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCa                                               >  1:630252/1‑100 (MQ=255)
                                                       tGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAAt                                             >  2:2283874/1‑100 (MQ=255)
                                                          gcTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGc                                         >  1:3005101/1‑100 (MQ=255)
                                                            tACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGcc                                        <  2:3345095/100‑1 (MQ=255)
                                                              cGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAg                                      <  1:1200664/100‑1 (MQ=255)
                                                              cGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAg                                      <  2:151653/100‑1 (MQ=255)
                                                              cGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAg                                      >  1:1539714/1‑100 (MQ=255)
                                                              cGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGc                                     >  2:515146/1‑100 (MQ=255)
                                                                cTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCa                                    <  2:3456811/100‑1 (MQ=255)
                                                                    tCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATa                                 >  1:3148546/1‑99 (MQ=255)
                                                                      aGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTg                              <  1:783301/100‑1 (MQ=255)
                                                                      aGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGa                             >  2:1637326/1‑100 (MQ=255)
                                                                       ggCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGa                             <  2:1539729/100‑1 (MQ=255)
                                                                       ggCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGa                             >  1:1075907/1‑100 (MQ=255)
                                                                       ggCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGa                             >  2:2799986/1‑100 (MQ=255)
                                                                       ggCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGaa                            >  1:1995618/1‑100 (MQ=255)
                                                                        gCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAAt                           <  2:1107967/100‑1 (MQ=255)
                                                                         ccTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAAt                           >  1:3282189/1‑100 (MQ=255)
                                                                          cTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCCGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTa                         >  1:1893455/1‑100 (MQ=255)
                                                                           tACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTa                         >  2:2953127/1‑100 (MQ=255)
                                                                           tACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTAc                        >  2:300718/1‑100 (MQ=255)
                                                                             cATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACg                       <  1:3333825/100‑1 (MQ=255)
                                                                             cATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACgg                      <  1:1370868/100‑1 (MQ=255)
                                                                             cATTTCATTGCAATATGTGCATTACTTTGTACGCCGGATAAGGAGTTAAAGCCGCAACCGGAATGAACAAAGCGTTATTTGACAGTAATAGCGAATTACg                       <  1:2886964/100‑1 (MQ=255)
                                                                              aTTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAATGCTTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGc                     <  1:2428172/100‑1 (MQ=25)
                                                                                  ccTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTc                  >  1:1505561/1‑100 (MQ=255)
                                                                                   cTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCg                 <  1:418993/100‑1 (MQ=255)
                                                                                   cTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCg                 <  2:630256/100‑1 (MQ=255)
                                                                                   cTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCAGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCg                 >  1:1730168/1‑100 (MQ=255)
                                                                                   cTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGc                >  2:306898/1‑100 (MQ=255)
                                                                                     tGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGcc               >  1:3009108/1‑100 (MQ=255)
                                                                                     tGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGcc               <  1:270680/100‑1 (MQ=255)
                                                                                     tGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGcc               <  2:1177220/100‑1 (MQ=255)
                                                                                     tGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCca              >  1:607985/1‑100 (MQ=255)
                                                                                      gCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCca              >  1:317504/1‑100 (MQ=255)
                                                                                          tatGTGCATTACTTTGTCGGCCGGATAAGGCGTTCCAGACGCATCCGGCATGAACAAAGCGCCATTTGCCAGCAATAGTGACTTACGGCTTCGCcac             <  2:2760209/97‑1 (MQ=25)
                                                                                          tatGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACaaa          <  1:3271324/100‑1 (MQ=255)
                                                                                           atGTGCATACTTTGTAGGCCGGATAAGGCGTTCATGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAAc        <  1:1829712/100‑1 (MQ=255)
                                                                                           atGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAAc        <  2:1537600/100‑1 (MQ=255)
                                                                                           atGTGCATACATTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAAc        <  1:2262555/100‑1 (MQ=255)
                                                                                            tgtgCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAAcc       <  1:1123614/100‑1 (MQ=255)
                                                                                            tgtgCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAAcc       <  2:740688/100‑1 (MQ=255)
                                                                                             gtgCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAAcc       >  1:3063194/1‑100 (MQ=255)
                                                                                               gCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCaa     >  1:2349878/1‑100 (MQ=255)
                                                                                                cATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCAAt    >  2:3195193/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCAATCG  >  NC_000913/3512462‑3512659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: