Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 380,022:1 (G)10→11 100% intergenic (‑141/+47) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0221.G68.3% 342.1 / 190.0 142intergenic (‑141/+47)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base . (26/19);  new base G (45/52);  total (71/71)
Fisher's exact test for biased strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

TCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGG  >  NC_000913/379925‑380121
                                                                                                    |                                                                                                   
tattctatatgtcaatgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCt                                                                                                   >  1:1786123‑M1/92‑100 (MQ=255)
     tatatgtcaatgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATc                                                                                               >  2:500485‑M1/87‑100 (MQ=255)
       tatgtcaatgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGc                                                                                            <  1:3094605‑M1/16‑1 (MQ=255)
         tgtcaatgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAg                                                                                          <  1:2977536‑M1/18‑1 (MQ=255)
           tcaatgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAggg                                                                                        <  2:1678540‑M1/20‑1 (MQ=255)
            caatgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAggg                                                                                        <  2:2040562‑M1/21‑1 (MQ=255)
              atgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGaa                                                                                      <  1:366825‑M1/23‑1 (MQ=255)
               tgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAgg                                                                                    >  1:564351‑M1/77‑100 (MQ=255)
                gcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAgg                                                                                    >  1:1780899‑M1/76‑100 (MQ=255)
                 catacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGgc                                                                                   >  1:2642064‑M1/75‑100 (MQ=255)
                     cccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGgccg                                                                                 <  1:2418047‑M1/28‑1 (MQ=255)
                       cccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc                                                                             <  1:3448912‑M1/32‑1 (MQ=255)
                         cctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                                           >  2:146891‑M1/67‑100 (MQ=255)
                          ctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa                                                                          >  2:1503993‑M1/66‑100 (MQ=255)
                          ctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa                                                                          >  1:472329‑M1/66‑100 (MQ=255)
                          ctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa                                                                          >  1:472495‑M1/66‑100 (MQ=255)
                           agcatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAg                                                                       >  1:1336106‑M2/8‑100 (MQ=255)
                               gtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg                                                                    <  1:3317670‑M1/40‑1 (MQ=255)
                                 atataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                                   <  2:2879496‑M1/42‑1 (MQ=255)
                                 atataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggc                                                                  <  1:122606‑M1/42‑1 (MQ=255)
                                  tataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcg                                                                 >  2:2977559‑M1/58‑100 (MQ=255)
                                  tataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcg                                                                 <  1:516370‑M1/43‑1 (MQ=255)
                                    taacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg                                                                <  1:400505‑M1/45‑1 (MQ=255)
                                     aacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                               <  2:542347‑M1/46‑1 (MQ=255)
                                       cactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAg                                                             <  1:2009930‑M1/48‑1 (MQ=255)
                                       cactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa                                                            >  2:516374‑M1/53‑100 (MQ=255)
                                        actggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa                                                            <  1:2069600‑M1/49‑1 (MQ=255)
                                         ctggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa                                                           >  1:917685‑M1/51‑100 (MQ=255)
                                         ctggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                                          <  1:1875976‑M1/50‑1 (MQ=255)
                                          tggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                                         >  2:3437080‑M1/50‑100 (MQ=255)
                                           ggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAaga                                                         <  2:3403928‑M1/52‑2 (MQ=255)
                                            gagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGaa                                                       <  1:1731323‑M1/53‑1 (MQ=255)
                                            gagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGaa                                                       >  2:2369693‑M1/48‑100 (MQ=255)
                                              gaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc                                                     <  1:732092‑M1/55‑1 (MQ=255)
                                                ataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa                                                    >  1:583613‑M1/44‑100 (MQ=255)
                                                 taaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg                                                   >  1:81871‑M1/43‑100 (MQ=255)
                                                 taaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg                                                   <  2:780163‑M1/58‑1 (MQ=255)
                                                  aaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa                                                  <  2:685948‑M1/59‑1 (MQ=255)
                                                  aaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa                                                  >  1:2835828‑M1/42‑100 (MQ=255)
                                                  aaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa                                                  >  1:1644461‑M1/42‑100 (MQ=255)
                                                   aaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa                                                 <  2:3111338‑M1/60‑1 (MQ=255)
                                                   aaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa                                                 <  1:2508412‑M1/60‑1 (MQ=255)
                                                   aaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGACGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa                                                 >  1:1295159‑M1/41‑100 (MQ=255)
                                                    aatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt                                                <  2:1015988‑M1/61‑1 (MQ=255)
                                                    aatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt                                                >  2:1758198‑M1/40‑100 (MQ=255)
                                                         atccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                                           <  1:1597129‑M1/66‑1 (MQ=255)
                                                           ccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                         <  1:601533‑M1/68‑1 (MQ=255)
                                                           ccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                         <  2:2496486‑M1/68‑1 (MQ=255)
                                                           ccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                         >  2:427721‑M1/33‑100 (MQ=255)
                                                            cggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                         <  2:1907949‑M1/68‑1 (MQ=255)
                                                             ggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                       >  1:3050922‑M1/31‑100 (MQ=255)
                                                             ggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                       <  2:2893926‑M1/70‑1 (MQ=255)
                                                             ggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                       <  1:94424‑M1/70‑1 (MQ=255)
                                                             ggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                                      <  1:2162861‑M1/70‑1 (MQ=255)
                                                              gtgaatgtggtcggaaaacaaagaggaaaGAGGCGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAGTTCCAGGTCa                                      <  1:2273547‑M1/71‑1 (MQ=255)
                                                                   tgtggccggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACgg                                 >  1:1660787‑M1/25‑100 (MQ=255)
                                                                    gtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACggg                                >  2:733546‑M1/24‑100 (MQ=255)
                                                                    gtgggcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACgg                                 >  1:1133790‑M1/24‑98 (MQ=255)
                                                                     tggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGc                               >  2:25771‑M1/23‑100 (MQ=255)
                                                                     tggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGc                               >  2:2248675‑M1/23‑100 (MQ=255)
                                                                       gtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTg                             <  2:1746157‑M1/80‑1 (MQ=255)
                                                                        tcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                            <  1:1600168‑M1/81‑1 (MQ=255)
                                                                         cggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa                           <  2:2562173‑M1/82‑1 (MQ=255)
                                                                         cggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa                           >  1:171246‑M1/19‑100 (MQ=255)
                                                                         cggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGGAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa                           >  2:2434213‑M1/19‑99 (MQ=255)
                                                                          ggaaatcaaagaggaaaGGGGGGGGCTAAGCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                            >  1:1857977‑M1/18‑98 (MQ=255)
                                                                          ggaaaacaaagaggaaaGGGGGAGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa                          <  1:890665‑M1/83‑1 (MQ=255)
                                                                          ggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa                          <  1:1048503‑M1/83‑1 (MQ=255)
                                                                          ggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa                          <  2:208980‑M1/83‑1 (MQ=255)
                                                                          ggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg                         <  2:1525587‑M1/83‑1 (MQ=255)
                                                                          ggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg                         >  1:2370546‑M1/18‑100 (MQ=255)
                                                                             cAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc                     >  1:416325/1‑100 (MQ=255)
                                                                             aaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGtt                       >  2:1393325‑M1/15‑100 (MQ=255)
                                                                             aaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGtt                       >  2:1303167‑M1/15‑100 (MQ=255)
                                                                              aacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg                      >  1:3281383‑M1/14‑100 (MQ=255)
                                                                               acaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc                     <  2:1535421‑M1/88‑1 (MQ=255)
                                                                               acaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                    >  2:639044‑M1/13‑100 (MQ=255)
                                                                                caaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                    <  2:1988136‑M1/89‑1 (MQ=255)
                                                                                caaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                    <  1:549197‑M1/89‑1 (MQ=255)
                                                                                caaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                    <  1:2977794‑M1/89‑1 (MQ=255)
                                                                                caaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                    <  1:3121794‑M1/89‑1 (MQ=255)
                                                                                caaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                    <  2:2790336‑M1/89‑1 (MQ=255)
                                                                                 aaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATGAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa                  <  1:3330023‑M1/90‑1 (MQ=255)
                                                                                  aagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa                  >  1:1048307‑M1/10‑100 (MQ=255)
                                                                                  aagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa                  >  1:2679555‑M1/10‑100 (MQ=255)
                                                                                     ggaagggggggggCTAATCGGGAGGGATGGCCGGACCGGATAGCGGACGGCAGAAGGAATCAGAATTTCCAGGTGGGACGGTCTGCAACTTGCAGCCCGt              <  1:982753/96‑1 (MQ=255)
                                                                                     ggaaaggggggggCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGt              >  2:2750767/6‑100 (MQ=255)
                                                                                     ggaaaggggggggCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGt              >  1:1275028/6‑100 (MQ=255)
                                                                                     ggaaaggggggggCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGt              >  2:585333/6‑100 (MQ=255)
                                                                                     aggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGTCTGCAACTTGCAGCCCg               >  1:1341836‑M1/7‑100 (MQ=255)
                                                                                      ggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGtt             <  2:1550851‑M1/95‑1 (MQ=255)
                                                                                        aaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaa           >  2:1078329‑M1/4‑100 (MQ=255)
                                                                                         aggggggggCTAATCGGCAGGGAAGGCAGCCCCGGATAGCGGGCGGCAGAAGGAATAAGAATTTCCAGGTCAGACGGGCTGCAAGTTGAAGACCGTTaaa          >  1:3221181/2‑100 (MQ=255)
                                                                                          ggaGGGTGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaaa         >  2:1030067‑M1/4‑100 (MQ=255)
                                                                                           GGGGGGGCTAATCGGCAGGGAAGGCGGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaaa         >  1:3127054‑M1/1‑99 (MQ=255)
                                                                                           GGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTACAAGTTGCAGACCGTTAAAat        >  1:2138820‑M1/1‑100 (MQ=255)
                                                                                            GGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatc       >  2:1622246‑M1/1‑100 (MQ=255)
                                                                                              GGGGCTAATCCGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAGGGGCTGCAAGTTGCAGACCGTTAAAatcat     >  1:2267988‑M1/1‑100 (MQ=255)
                                                                                                  GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg   >  2:2366794‑M1/1‑100 (MQ=255)
                                                                                                   GCTAATCGGCAGGGAAGGCCGCCGCGGATAGCGGGCGGCAGAAGGAATCAGAACTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGGTAAAATCATCgg  <  1:285551‑M1/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
TCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGG  >  NC_000913/379925‑380121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: