Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 99,399 G→A 15.4% A333T (GCA→ACA)  ftsW → putative lipid II flippase; integral membrane protein; FtsZ ring stabilizer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091399,3990GA15.4% 321.8 / 45.4 130A333T (GCA→ACA) ftsWputative lipid II flippase; integral membrane protein; FtsZ ring stabilizer
Reads supporting (aligned to +/- strand):  ref base G (60/49);  new base A (9/11);  total (70/60)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.44e-01

Only 100 of 132 total aligned reads displayed.

TTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCGGCGGCGGGGA  >  NC_000913/99300‑99498
                                                                                                   |                                                                                                   
ttCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAg                                                                                                     >  1:2010970/1‑100 (MQ=255)
 tctccATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGc                                                                                                    <  1:1454116/97‑1 (MQ=255)
 tCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGc                                                                                                    <  2:3128186/100‑1 (MQ=255)
    ccATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCaga                                                                                                 >  2:1454129/1‑98 (MQ=255)
         aTCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGaaa                                                                                            >  1:1033994/1‑100 (MQ=255)
         aTCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGaa                                                                                             >  1:3018443/1‑99 (MQ=255)
          tCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAAt                                                                                           <  2:180532/100‑1 (MQ=255)
            ggCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTg                                                                                         >  2:3101669/1‑100 (MQ=255)
             gCGAAGAACTGGGGTATGTCGTTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGa                                                                                        <  1:1512722/100‑1 (MQ=255)
             gCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGa                                                                                        <  1:2900841/100‑1 (MQ=255)
             gCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGa                                                                                        <  2:1427182/100‑1 (MQ=255)
             gCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAACATTAGAAATTGa                                                                                        <  1:2712821/100‑1 (MQ=255)
              cGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGac                                                                                       >  1:3312520/1‑100 (MQ=255)
              cGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGac                                                                                       <  2:97086/100‑1 (MQ=255)
               gaagaaCTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGacc                                                                                      >  1:1733961/1‑100 (MQ=255)
                aagaaCTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGacca                                                                                     <  2:174949/100‑1 (MQ=255)
                aagaaCTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGacca                                                                                     <  2:1885086/100‑1 (MQ=255)
                 agaaCTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGaccac                                                                                    >  2:1382193/1‑100 (MQ=255)
                 agaaCTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGaccac                                                                                    >  1:487732/1‑100 (MQ=255)
                 agaaCTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGaccac                                                                                    >  1:486844/1‑100 (MQ=255)
                     cTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGtt                                                                                <  2:2362863/100‑1 (MQ=255)
                       ggggTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGtttt                                                                              >  2:1602024/1‑100 (MQ=255)
                       ggggTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGtttt                                                                              >  1:2955576/1‑100 (MQ=255)
                       ggggTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGtttt                                                                              >  1:67587/1‑100 (MQ=255)
                        gggTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGttttt                                                                             >  2:383175/1‑100 (MQ=255)
                         ggTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTc                                                                            >  2:1309956/1‑100 (MQ=255)
                          ccgatctggTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTcc                                                                           <  1:2211664/93‑1 (MQ=255)
                            aTGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCgg                                                                         >  2:145337/1‑100 (MQ=255)
                            aTGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCgg                                                                         <  1:1167123/100‑1 (MQ=255)
                              gTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGtt                                                                       <  1:597647/100‑1 (MQ=255)
                               tCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGttt                                                                      <  2:487736/100‑1 (MQ=255)
                               tCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGttt                                                                      <  1:1081341/100‑1 (MQ=255)
                                 ggTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTtc                                                                    >  2:578689/1‑100 (MQ=255)
                                 ggTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTtc                                                                    >  2:2858909/1‑100 (MQ=255)
                                 ggTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTtc                                                                    <  2:447126/100‑1 (MQ=255)
                                 ggTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTtc                                                                    >  1:2918074/1‑100 (MQ=255)
                                 ggTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAACATTAGAAATTGACCACCGTTTTTCCGGTTTtc                                                                    >  2:1679044/1‑100 (MQ=255)
                                   tgtgGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTtctc                                                                  <  1:3078227/100‑1 (MQ=255)
                                    gtggtgCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCg                                                                 >  1:1553188/1‑100 (MQ=255)
                                    gtggtgCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCg                                                                 <  2:2715748/100‑1 (MQ=255)
                                    gtggtgCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCg                                                                 >  2:32927/1‑100 (MQ=255)
                                    gtggtgCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCg                                                                 <  1:106602/100‑1 (MQ=255)
                                      ggtgCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGcc                                                               <  1:301676/100‑1 (MQ=255)
                                      ggtgCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGcc                                                               <  1:1636635/100‑1 (MQ=255)
                                         gCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGt                                                            >  2:1617446/1‑100 (MQ=255)
                                         gCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAACATTAGAAATTGACCACCGTTTTTCCGGGTTTCTCGCCTGt                                                            >  2:2552952/1‑100 (MQ=255)
                                          cTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGAACACCGTTTTTCCGGTTTTCTCGCCTGtt                                                           >  1:2574335/1‑100 (MQ=255)
                                           tGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTc                                                          <  2:1553203/100‑1 (MQ=255)
                                           tGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTc                                                          <  2:1555265/100‑1 (MQ=255)
                                               aCTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTAtt                                                      <  1:2852735/100‑1 (MQ=255)
                                                cTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTg                                                     <  1:373391/100‑1 (MQ=255)
                                                cTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTg                                                     <  1:1986100/100‑1 (MQ=255)
                                                cTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTg                                                     <  2:867886/100‑1 (MQ=255)
                                                cTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAACATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTg                                                     <  1:2312721/100‑1 (MQ=255)
                                                 ttttAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGCCCACCGTTTTTCCGGTTTTCTCGCCTGTTCTAt                                                       >  1:2614303/1‑97 (MQ=255)
                                                  tttAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGc                                                   >  2:3199134/1‑100 (MQ=255)
                                                  tttAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGc                                                   >  2:2185185/1‑100 (MQ=255)
                                                  tttAATGGTATTCTTCGTCGCTTTGCGCGCGATGTCGATTGGCCGTAAAACATTAGTAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTgg                                                    <  1:1305889/99‑1 (MQ=255)
                                                    tAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCAt                                                 >  1:1624258/1‑100 (MQ=255)
                                                     aaTGGTATTCTTCGTCGCTGTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCAt                                                 >  1:844797/1‑99 (MQ=255)
                                                        ggTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAACATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTgg                                             >  2:508999/1‑100 (MQ=255)
                                                           aTTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGttt                                          >  1:2518997/1‑100 (MQ=255)
                                                               ttcGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCt                                      <  1:1309944/100‑1 (MQ=255)
                                                                  gtcgCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTcc                                   <  1:2548705/100‑1 (MQ=255)
                                                                  gtcgCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTcc                                   >  2:1103394/1‑100 (MQ=255)
                                                                   tcgCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCa                                  <  1:32927/100‑1 (MQ=255)
                                                                   tcgCTTTTCGCGCGATGTCGATTGGCCGTAAAACATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCa                                  <  2:2140882/100‑1 (MQ=255)
                                                                     gCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAgg                                >  2:741229/1‑100 (MQ=255)
                                                                      cTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAgg                                >  1:1247061/1‑99 (MQ=255)
                                                                      cTTTTCGCGCGATGTCGATTGGCCGTAAAACATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGgc                               >  2:3459595/1‑100 (MQ=255)
                                                                      cTTTTCGCGCGATGTCGATTGGCCGTAAAACATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGgc                               >  2:1032058/1‑100 (MQ=255)
                                                                         ttCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCt                            <  1:2858886/100‑1 (MQ=255)
                                                                          tCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTg                           >  1:528010/1‑100 (MQ=255)
                                                                           cgcgcgATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTgg                          <  2:436453/100‑1 (MQ=255)
                                                                           cgcgcgATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTg                           >  1:3225911/1‑99 (MQ=255)
                                                                           cgcgcgATGTCGATTGGCCGTAAAACATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTgg                          >  1:2105659/1‑100 (MQ=255)
                                                                            gcgcgGTGTCGATTAGCCGTAGAGCATTAGAAATTGACCACCGTTTTGCCGGTTTTCTCGCCTGTTCTATTGGCGTCTGGTTTGGCTTCCATGCGCTGGt                         >  1:2886321/1‑100 (MQ=255)
                                                                            gcgcgATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGt                         <  1:1108711/100‑1 (MQ=255)
                                                                              gcgATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTa                       >  1:1286224/1‑100 (MQ=255)
                                                                              gcgATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTa                       >  1:1570381/1‑100 (MQ=255)
                                                                              gcgATGTCGATTGGCCGTAAAACATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTa                       <  2:2241014/100‑1 (MQ=255)
                                                                              gcgATGTCGATTGGCCGTAAAACATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTa                       >  1:2104213/1‑100 (MQ=255)
                                                                                gATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAAc                     <  1:1975265/100‑1 (MQ=255)
                                                                                 aTGTCGATTGGCCGTAAAACATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACg                    <  2:525198/100‑1 (MQ=255)
                                                                                  tGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGt                   >  1:806032/1‑100 (MQ=255)
                                                                                  tGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGt                   >  2:1455853/1‑100 (MQ=255)
                                                                                   gTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCTGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTa                  <  2:2890420/100‑1 (MQ=255)
                                                                                    tCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAg                 >  2:2047979/1‑100 (MQ=255)
                                                                                     cGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAgg                >  2:1047985/1‑100 (MQ=255)
                                                                                     cGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAgg                <  2:63889/100‑1 (MQ=255)
                                                                                          ggCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCgcgg           >  2:460651/1‑100 (MQ=255)
                                                                                          ggCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCgcgg           >  1:2581607/1‑100 (MQ=255)
                                                                                          ggCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCgcgg           >  2:1543900/1‑100 (MQ=255)
                                                                                           gCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCATGCGCTGGTTAACGTAGGCgcggc          >  1:636351/1‑100 (MQ=255)
                                                                                            ccGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCgcggcg         >  1:277555/1‑100 (MQ=255)
                                                                                                aaaGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGcggcggcgg     >  1:1993240/1‑100 (MQ=255)
                                                                                                 aaGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCGGCGGCggg    <  2:551573/100‑1 (MQ=255)
                                                                                                  aGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCGGCGGCgggg   <  2:2225496/100‑1 (MQ=255)
                                                                                                  aGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCGGCGGCgggg   >  1:2297698/1‑100 (MQ=255)
                                                                                                   gCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCGGCGGCGGGGa  >  2:1164374/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCGGCGGCGGGGA  >  NC_000913/99300‑99498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: