Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 715,849 A→G 32.6% L146L (TTA→TTG)  ybfP → lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913715,8490AG32.6% 141.6 / 100.7 116L146L (TTA→TTG) ybfPlipoprotein
Reads supporting (aligned to +/- strand):  ref base A (37/41);  new base G (16/22);  total (53/63)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

GCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTT  >  NC_000913/715752‑715948
                                                                                                 |                                                                                                   
gCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTacg                                                                                                   <  2:2574662/100‑1 (MQ=255)
  cAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTacgac                                                                                                 >  2:944436/1‑100 (MQ=255)
     ggCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCt                                                                                              <  2:3329037/100‑1 (MQ=255)
      gCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTg                                                                                             <  2:1559069/100‑1 (MQ=255)
       cAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGc                                                                                            <  1:3357150/100‑1 (MQ=255)
        tgccctggCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGcc                                                                                           <  2:750587/94‑1 (MQ=255)
        aaGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGcc                                                                                           <  2:2700692/100‑1 (MQ=255)
        aaGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGcc                                                                                           <  2:1885113/100‑1 (MQ=255)
          gCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTTTTTTTTTTTTGCATGTCGGGATTGTTGAGTTACGACTCTGCCAg                                                                                         >  1:841566/1‑100 (MQ=255)
          gCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAg                                                                                         <  2:3225574/100‑1 (MQ=255)
          gCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAg                                                                                         <  2:1946628/100‑1 (MQ=255)
            ccGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTc                                                                                       <  2:667065/100‑1 (MQ=255)
                  cTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGaaaa                                                                                 <  2:2146339/100‑1 (MQ=255)
                   tCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAAt                                                                                >  1:2820553/1‑100 (MQ=255)
                     gACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTg                                                                              <  1:3212246/100‑1 (MQ=255)
                       ccGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTTTCTTGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGcc                                                                            >  1:3071648/1‑100 (MQ=255)
                       ccGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGcc                                                                            <  1:1049867/100‑1 (MQ=255)
                       ccGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGcc                                                                            <  1:1674523/100‑1 (MQ=255)
                       ccGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGcc                                                                            <  2:201336/100‑1 (MQ=255)
                        cGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCa                                                                           <  1:2717095/100‑1 (MQ=255)
                         gATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAg                                                                          <  1:2840926/100‑1 (MQ=255)
                          aTAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGc                                                                         >  1:2940157/1‑100 (MQ=255)
                           ttgTCAATGAGGATTATGTCAGCGAAAGCGCATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCGGCCAGACAGAAAATTGCCAGCa                                                                        <  1:2097581/98‑1 (MQ=255)
                           tAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAGATTGCCAGCa                                                                        >  1:2264410/1‑100 (MQ=255)
                           tAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCa                                                                        >  2:366019/1‑100 (MQ=255)
                            aGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCaa                                                                       >  2:3120287/1‑100 (MQ=255)
                               cAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGaa                                                                    >  2:1774658/1‑100 (MQ=255)
                                aaTGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAAt                                                                   >  1:2534184/1‑100 (MQ=255)
                                 aTGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAAtt                                                                  >  1:2122256/1‑100 (MQ=255)
                                  tGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTg                                                                 >  2:1528717/1‑100 (MQ=255)
                                  tGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTg                                                                 <  2:1077391/100‑1 (MQ=255)
                                  tGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCAGGATTATTGAGTTGCTACGCTGCCAGTCAGAAAATTGCCAGCAAGAAtt                                                                  <  1:1147573/99‑1 (MQ=255)
                                   gAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGATGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGa                                                                <  1:2270229/100‑1 (MQ=255)
                                   gAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGa                                                                <  1:1755549/100‑1 (MQ=255)
                                   gAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGa                                                                >  2:2126592/1‑100 (MQ=255)
                                    aGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaa                                                               <  2:2862876/100‑1 (MQ=255)
                                    aGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGAGTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaa                                                               <  2:2660805/100‑1 (MQ=255)
                                      gATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAg                                                             >  2:245238/1‑100 (MQ=255)
                                      gATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAg                                                             <  2:2485143/100‑1 (MQ=255)
                                      gATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAg                                                             <  2:2899959/100‑1 (MQ=255)
                                        ttATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCg                                                           <  1:2361443/100‑1 (MQ=255)
                                        ttATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCg                                                           >  2:1605628/1‑100 (MQ=255)
                                        ttATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATACATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCg                                                           <  1:1097926/100‑1 (MQ=255)
                                          aTGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGAt                                                         >  1:79783/1‑100 (MQ=255)
                                           tGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATa                                                        <  1:3081894/100‑1 (MQ=255)
                                            gTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATaa                                                       >  2:1131717/1‑100 (MQ=255)
                                            gTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATaa                                                       <  1:2056318/100‑1 (MQ=255)
                                             tCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATaaa                                                      >  2:1747830/1‑100 (MQ=255)
                                                gCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATac                                                   >  1:2721153/1‑100 (MQ=255)
                                                gCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATac                                                   <  1:389175/100‑1 (MQ=255)
                                                 cGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATaca                                                  >  2:2874206/1‑100 (MQ=255)
                                                 cGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATaca                                                  >  2:250943/1‑100 (MQ=255)
                                                     aGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACAtt                                              >  2:3195630/1‑100 (MQ=255)
                                                      gCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTa                                             <  2:2714485/100‑1 (MQ=255)
                                                       cGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTaa                                            <  1:3371211/100‑1 (MQ=255)
                                                       cGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTaa                                            <  2:1438769/100‑1 (MQ=255)
                                                       cGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGATGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTaa                                            >  2:3319681/1‑100 (MQ=255)
                                                           ttttttGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATa                                        <  1:2384212/100‑1 (MQ=255)
                                                             ttttGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGt                                      <  1:1642676/100‑1 (MQ=255)
                                                             ttttGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGt                                      <  2:649814/100‑1 (MQ=255)
                                                             ttttGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGt                                      <  2:750624/100‑1 (MQ=255)
                                                              tttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                     <  2:2685917/100‑1 (MQ=255)
                                                              tttGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                     <  2:3386644/100‑1 (MQ=255)
                                                              tttGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                     <  2:1159802/100‑1 (MQ=255)
                                                               ttGGCTCGATGATGCATGTCGGGATGATTGAGTTACGAGGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                     <  1:1313366/99‑1 (MQ=255)
                                                                tGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAg                                   >  1:566241/1‑100 (MQ=255)
                                                                  gCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTc                                 >  1:597797/1‑100 (MQ=255)
                                                                   cTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTcc                                >  2:3144529/1‑100 (MQ=255)
                                                                   cTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTcc                                >  1:2142433/1‑100 (MQ=255)
                                                                   cTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGACAAATACACATTAAAATAGTGAGTcc                                >  1:1280912/1‑100 (MQ=255)
                                                                       atgatgCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGa                            <  2:3131029/100‑1 (MQ=255)
                                                                         gatgCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAAt                          <  1:2753355/100‑1 (MQ=255)
                                                                         gatgCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAAt                          <  2:1058942/100‑1 (MQ=255)
                                                                           tgCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGa                        >  2:1734250/1‑100 (MQ=255)
                                                                           tgCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGa                        >  1:2349175/1‑100 (MQ=255)
                                                                           tgCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGa                        >  2:2847950/1‑100 (MQ=255)
                                                                            gCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGaa                       >  2:627423/1‑100 (MQ=255)
                                                                              aTGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAc                     <  2:2755544/100‑1 (MQ=255)
                                                                               tGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCgggtttcattcc                    <  2:196459/100‑10 (MQ=255)
                                                                                 tCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCg                  >  1:2655158/1‑100 (MQ=255)
                                                                                 tCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAccc                   >  2:375381/1‑99 (MQ=255)
                                                                                 tCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCg                  >  2:3317018/1‑100 (MQ=255)
                                                                                    ggATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAc               <  2:1090976/100‑1 (MQ=255)
                                                                                    ggATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGan               <  2:1601003/100‑2 (MQ=255)
                                                                                    ggATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAc               <  2:2837688/100‑1 (MQ=255)
                                                                                     gATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAcc              <  1:1622093/100‑1 (MQ=255)
                                                                                     gATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAcc              <  1:338201/100‑1 (MQ=255)
                                                                                     gATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAcc              <  2:1629611/100‑1 (MQ=255)
                                                                                      attattGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCt             >  1:2238603/1‑100 (MQ=255)
                                                                                        tattGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGa           >  1:2895706/1‑100 (MQ=255)
                                                                                         attGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGACAGCGATAAATACACATTAAAATAGTGAGTACGGGAATGAAACCCCGACCTGa           >  1:175073/1‑99 (MQ=255)
                                                                                         attGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGaa          >  2:912079/1‑100 (MQ=255)
                                                                                         attGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGaa          <  1:997022/100‑1 (MQ=255)
                                                                                          ttGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAAc         <  1:2920318/100‑1 (MQ=255)
                                                                                           tGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACa        <  2:2264428/100‑1 (MQ=255)
                                                                                           tGAGTTACGACGCTGCCAGTCAGAACATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAAcn        <  1:192/100‑2 (MQ=255)
                                                                                           tGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACa        >  2:489593/1‑100 (MQ=255)
                                                                                            gAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACAt       >  2:2951329/1‑100 (MQ=255)
                                                                                             aGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCTGGACCTGAACATg      <  1:2141546/100‑1 (MQ=255)
                                                                                                 acgacgCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCtt  >  1:1959517/1‑100 (MQ=255)
                                                                                                 |                                                                                                   
GCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGTCTT  >  NC_000913/715752‑715948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑