Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,197,853 T→C 36.7% N129D (AAT→GAT)  ymfE ← e14 prophage; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,197,8530TC36.7% 121.4 / 142.0 136N129D (AAT→GAT) ymfEe14 prophage; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (45/41);  new base C (26/24);  total (71/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

GAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCG  >  NC_000913/1197756‑1197952
                                                                                                 |                                                                                                   
gAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGc                                                                                                   >  2:1455607/1‑100 (MQ=255)
gAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGc                                                                                                   <  1:35830/100‑1 (MQ=255)
  aaaTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTc                                                                                                 <  1:819145/100‑1 (MQ=255)
  aaaTTAAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGc                                                                                                   <  1:1484061/98‑1 (MQ=255)
   aaTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCa                                                                                                <  1:1261694/100‑1 (MQ=255)
    aTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCAt                                                                                               <  1:266849/100‑1 (MQ=255)
    aTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTATGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCAt                                                                                               >  1:2214373/1‑100 (MQ=255)
      tCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTa                                                                                             >  2:2366741/1‑100 (MQ=255)
       ctaaataTATCTGAAAATTTTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCAtt                                                                                              >  1:2984663/3‑98 (MQ=255)
       cAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTAc                                                                                            >  1:2479515/1‑100 (MQ=255)
       cAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTAc                                                                                            <  2:47148/100‑1 (MQ=255)
        aaaaaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACa                                                                                           >  1:1811606/1‑100 (MQ=255)
         aaaaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACAt                                                                                          <  2:3038487/100‑1 (MQ=255)
                 ccAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCt                                                                                  <  2:605938/100‑1 (MQ=255)
                  cAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCtt                                                                                 >  2:1096886/1‑100 (MQ=255)
                  cAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCtt                                                                                 >  2:1438874/1‑100 (MQ=255)
                    aaaaTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCa                                                                               <  1:1307310/100‑1 (MQ=255)
                    aaaaTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTAAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCa                                                                               >  1:1666854/1‑100 (MQ=255)
                       aTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCt                                                                            <  1:1276053/100‑1 (MQ=255)
                        tAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtt                                                                           <  1:1165883/100‑1 (MQ=255)
                          gTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCttta                                                                         <  2:670615/100‑1 (MQ=255)
                          gTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCttta                                                                         >  1:1679775/1‑100 (MQ=255)
                          gTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCttt                                                                          >  2:3159297/1‑99 (MQ=255)
                           tataCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCtttat                                                                        <  1:421807/100‑1 (MQ=255)
                            ataCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtttatt                                                                       >  2:2166801/1‑100 (MQ=255)
                             taCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCGGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtttattt                                                                      <  1:2318458/100‑1 (MQ=255)
                              aCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCtttattta                                                                     >  1:2198714/1‑100 (MQ=255)
                              aCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCtttattta                                                                     >  1:1322926/1‑100 (MQ=255)
                               cTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTaa                                                                    >  2:1169706/1‑100 (MQ=255)
                               cTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTaa                                                                    >  2:2941638/1‑100 (MQ=255)
                               cTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTaa                                                                    >  2:3323897/1‑100 (MQ=255)
                                 ttATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAAtt                                                                  <  2:2672523/100‑1 (MQ=255)
                                 ttATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAAtt                                                                  <  2:2656085/100‑1 (MQ=255)
                                  tATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAAttt                                                                 <  2:861234/100‑1 (MQ=255)
                                    ttCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTc                                                               >  2:675130/1‑100 (MQ=255)
                                     tCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCt                                                              <  1:1048965/100‑1 (MQ=255)
                                     tCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCt                                                              >  1:2626409/1‑100 (MQ=255)
                                      ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTg                                                             <  2:2990281/100‑1 (MQ=255)
                                      ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTg                                                             <  2:3223338/100‑1 (MQ=255)
                                      ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTcg                                                              >  1:2106060/1‑98 (MQ=255)
                                      ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCt                                                              >  2:3245267/1‑99 (MQ=255)
                                       cAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGt                                                            >  2:334551/1‑100 (MQ=255)
                                         gaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTg                                                          >  2:2155126/1‑100 (MQ=255)
                                         gaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTg                                                          >  1:1963361/1‑100 (MQ=255)
                                           agagTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGAc                                                        <  1:2442778/100‑1 (MQ=255)
                                              gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTgagatcgga                                                     >  1:2889726/1‑92 (MQ=255)
                                              gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTcc                                                     <  1:2801424/100‑1 (MQ=255)
                                              gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTcc                                                     >  1:1836216/1‑100 (MQ=255)
                                              gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTcc                                                     >  2:3430536/1‑100 (MQ=255)
                                              gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTcc                                                     >  2:2534325/1‑100 (MQ=255)
                                              gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTcc                                                     <  1:463596/100‑1 (MQ=255)
                                              gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTcc                                                     <  1:2142869/100‑1 (MQ=255)
                                                 cAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGc                                                  >  2:806017/1‑100 (MQ=255)
                                                 cAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGc                                                  <  2:870739/100‑1 (MQ=255)
                                                   atatAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGctc                                                >  1:1631063/1‑100 (MQ=255)
                                                      taATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTTCAGCTCTcc                                             >  1:711935/1‑100 (MQ=255)
                                                      taATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTcc                                             >  2:2889358/1‑100 (MQ=255)
                                                        gcgaccgacTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGt                                           <  2:2233278/92‑1 (MQ=255)
                                                        aTGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGt                                           <  2:451235/100‑1 (MQ=255)
                                                         tGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTg                                          <  2:2099403/100‑1 (MQ=255)
                                                         tGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTg                                          >  1:2841814/1‑100 (MQ=255)
                                                          gTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGa                                         <  2:1723152/100‑1 (MQ=255)
                                                             tGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATaa                                      >  2:2461421/1‑100 (MQ=255)
                                                             tGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATaa                                      >  2:2458530/1‑100 (MQ=255)
                                                              gTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAAc                                     <  1:1688346/100‑1 (MQ=255)
                                                              gTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAAc                                     >  2:2140521/1‑100 (MQ=255)
                                                                gttcgttttttCTTGCTTCAGGGTAATATAGTTTTCTCATTACTTTTTGAGCTTCATCTTTAGTTAATATTCGGTTGTCTGCAGCTCTCCGTGATAACgg                                   >  1:3232952/7‑100 (MQ=255)
                                                                cTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGCTAACgg                                   >  1:1976267/1‑100 (MQ=255)
                                                                cTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACgg                                   >  2:2401393/1‑100 (MQ=255)
                                                                cTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACgg                                   >  2:1951341/1‑100 (MQ=255)
                                                                cTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACgg                                   >  1:594457/1‑100 (MQ=255)
                                                                cTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACgg                                   <  1:3234205/100‑1 (MQ=255)
                                                                 ttCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTAAATTATAAGCTTCATCTTTATATAATTTTCTGTTGACTCCAGCTCTCCGTAATAACgat                                  >  1:878498/1‑98 (MQ=255)
                                                                  tCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGtt                                 >  2:2613746/1‑100 (MQ=255)
                                                                   cAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTGATCTTTATTTAATTTTCTGTTGACTCAAGCTCTCCGTGATAACGGttt                                >  1:2688534/1‑100 (MQ=255)
                                                                   cAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGttt                                <  1:1730829/100‑1 (MQ=255)
                                                                   cAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGttt                                <  1:3412557/100‑1 (MQ=255)
                                                                     aTTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTta                              <  2:1181263/100‑1 (MQ=255)
                                                                            ttACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAg                       <  2:1267220/100‑1 (MQ=255)
                                                                             tACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAgn                      <  2:3032287/100‑2 (MQ=255)
                                                                              aCTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGAt                     >  2:1230447/1‑100 (MQ=255)
                                                                                    gggTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTAt               >  1:1900765/1‑100 (MQ=255)
                                                                                      gTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATcc             >  2:703626/1‑100 (MQ=255)
                                                                                      gTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATcc             <  2:2984687/100‑1 (MQ=255)
                                                                                      gTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATcc             <  1:2388763/100‑1 (MQ=255)
                                                                                       tAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATccc            <  1:3159273/100‑1 (MQ=255)
                                                                                       tAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATccc            <  1:1096877/100‑1 (MQ=255)
                                                                                        aaTATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCa           >  1:2525428/1‑100 (MQ=255)
                                                                                         atatAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCaa          <  2:3267294/100‑1 (MQ=255)
                                                                                          tataGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATATTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCaaa         <  1:2433242/100‑1 (MQ=255)
                                                                                           ataGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAg        >  2:3378758/1‑100 (MQ=255)
                                                                                           ataGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAg        <  2:1368728/100‑1 (MQ=255)
                                                                                           ataGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAg        >  2:2811480/1‑100 (MQ=255)
                                                                                            taGATCGCTCTTTACATTGTGAGCTTTATCTTTATTTAATTTTCTGTTGTCTTCAGCTCTCCGTGATAACGGTTTTATAATTATATGCTTATCCCAAAg        <  1:675126/99‑1 (MQ=255)
                                                                                            taGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGa       >  2:3120563/1‑100 (MQ=255)
                                                                                                ttGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATc   <  2:758178/100‑1 (MQ=255)
                                                                                                ttGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATc   <  1:1448458/100‑1 (MQ=255)
                                                                                                 tGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATCATTAGATGCTTATCCCAAAGATATCg  <  1:1547477/100‑1 (MQ=255)
                                                                                                 tGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCg  <  2:1836232/100‑1 (MQ=255)
                                                                                                 tGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCg  <  2:1631079/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
GAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATATCG  >  NC_000913/1197756‑1197952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: