Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,411,512 G→A 32.1% A146V (GCG→GTG)  ttcA ← tRNA s(2)C32 thioltransferase, iron sulfur cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,411,5120GA32.1% 157.9 / 98.9 118A146V (GCG→GTG) ttcAtRNA s(2)C32 thioltransferase, iron sulfur cluster protein
Reads supporting (aligned to +/- strand):  ref base G (42/38);  new base A (19/19);  total (61/57)
Fisher's exact test for biased strand distribution p-value = 8.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01

Only 100 of 122 total aligned reads displayed.

CATCGCTCATCAGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCT  >  NC_000913/1411414‑1411611
                                                                                                  |                                                                                                   
 atcGCTCATCAGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAAcgcg                                                                                                   >  2:1778396/1‑100 (MQ=255)
 atcGCTCATCAGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAAcacg                                                                                                   <  2:3436177/100‑1 (MQ=255)
  tcGCTCATCAGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGa                                                                                                  <  2:2919712/100‑1 (MQ=255)
      tcatcaGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCtt                                                                                              <  2:1940430/100‑1 (MQ=255)
       catcaGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTtc                                                                                             >  1:2116589/1‑100 (MQ=255)
       catcaGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTtc                                                                                             >  2:820758/1‑100 (MQ=255)
        atcaGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTtcg                                                                                            >  2:898329/1‑100 (MQ=255)
         tcaGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTtcgt                                                                                           >  1:1872313/1‑100 (MQ=255)
           attttCGGATGCATACCTTGCATCGTGCCGCCGTAGAACATATTTAAGAACAACGTTGGCAGGATATCGTCACGATGGGGACCCAACACGATCTtcgtc                                                                                          <  1:2418247/97‑1 (MQ=255)
           aGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTtcgtcg                                                                                         >  1:607289/1‑100 (MQ=255)
           aGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTtcgtcg                                                                                         <  2:135719/100‑1 (MQ=255)
           aGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTtcgtcg                                                                                         <  2:2397927/100‑1 (MQ=255)
            gTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGc                                                                                        <  2:3429522/100‑1 (MQ=255)
            gTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGc                                                                                        >  1:3236092/1‑100 (MQ=255)
              ttCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGccc                                                                                      <  1:2342967/100‑1 (MQ=255)
              ttCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGccc                                                                                      >  2:3366106/1‑100 (MQ=255)
                cGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGccccc                                                                                    >  2:1295984/1‑100 (MQ=255)
                cGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGccccc                                                                                    <  2:2857057/100‑1 (MQ=255)
                cGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGccccc                                                                                    >  1:172044/1‑100 (MQ=255)
                 ggAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCa                                                                                   <  1:3147084/100‑1 (MQ=255)
                  gAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAg                                                                                  <  1:1998328/100‑1 (MQ=255)
                      cATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTcc                                                                              >  1:795558/1‑100 (MQ=255)
                       aTACCTTTCATCTTACCGCCATAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCg                                                                             >  1:3038592/1‑100 (MQ=255)
                          ccTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTg                                                                          >  2:1962223/1‑100 (MQ=255)
                           cTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGc                                                                         >  1:1425482/1‑100 (MQ=255)
                           cTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGc                                                                         >  1:1482964/1‑100 (MQ=255)
                           cTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGc                                                                         >  2:390807/1‑100 (MQ=255)
                           cTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGc                                                                         <  2:2775928/100‑1 (MQ=255)
                            tttCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCg                                                                        <  2:1516554/100‑1 (MQ=255)
                             ttCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCgg                                                                       <  2:1749350/100‑1 (MQ=255)
                             ttCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCgg                                                                       <  1:346846/100‑1 (MQ=255)
                             ttCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCgg                                                                       <  2:509718/100‑1 (MQ=255)
                              tCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGt                                                                      <  2:1239326/100‑1 (MQ=255)
                               cATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTa                                                                     >  1:1576065/1‑100 (MQ=255)
                               cATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTa                                                                     >  1:3204245/1‑100 (MQ=255)
                                aTCTTACCGCCGTAGCACATATTTAAGTACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTAc                                                                    <  2:48191/100‑1 (MQ=255)
                                aTCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTAc                                                                    >  2:1670355/1‑100 (MQ=255)
                                 tCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACg                                                                   <  2:264642/100‑1 (MQ=255)
                                 tCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACg                                                                   >  1:1618904/1‑100 (MQ=255)
                                 tCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACg                                                                   <  2:1349231/100‑1 (MQ=255)
                                 tCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACg                                                                   <  1:691048/100‑1 (MQ=255)
                                  cTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGa                                                                  <  2:797060/100‑1 (MQ=255)
                                      ccgccgTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATaaa                                                              >  1:615118/1‑100 (MQ=255)
                                      ccgccgTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATaaa                                                              >  1:1422385/1‑100 (MQ=255)
                                       cgccgTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAg                                                             <  2:1088701/100‑1 (MQ=255)
                                              gAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACcg                                                      <  1:2789127/100‑1 (MQ=255)
                                                 cATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcga                                                   <  2:1163516/100‑1 (MQ=255)
                                                  atatTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgac                                                  <  2:214103/100‑1 (MQ=255)
                                                   tatTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgacg                                                 >  1:2337422/1‑100 (MQ=255)
                                                    atTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgacga                                                >  1:535949/1‑100 (MQ=255)
                                                       tAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAgg                                             <  1:1669524/100‑1 (MQ=255)
                                                       tAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGc                                            >  1:2367213/1‑100 (MQ=255)
                                                       tAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAgg                                             <  1:3093598/100‑1 (MQ=255)
                                                         aGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCg                                           >  2:825159/1‑100 (MQ=255)
                                                         aGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCg                                           <  1:2256432/100‑1 (MQ=255)
                                                          gAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCga                                          >  2:385217/1‑100 (MQ=255)
                                                          gAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCga                                          >  2:685408/1‑100 (MQ=255)
                                                          gAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCga                                          >  2:1420392/1‑100 (MQ=255)
                                                           aacaacGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCgag                                         <  1:1962207/100‑1 (MQ=255)
                                                            acaacGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCgaga                                        <  1:1904355/100‑1 (MQ=255)
                                                            acaacGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCgaga                                        <  2:2394571/100‑1 (MQ=255)
                                                             ccacGTTTGCAGGATCTCGTCACGATGGTGACCCAACGCGATATTCCTCGGCCCCAGTTCCGTTGTGGTAGGATAAAGGATACCGCGACGGAgg                                             >  1:3215117/3‑94 (MQ=255)
                                                             caacGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGaa                                       >  1:895928/1‑100 (MQ=255)
                                                             caacGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGaa                                       >  1:620299/1‑100 (MQ=255)
                                                              aacGTCTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAac                                      <  2:22059/100‑1 (MQ=255)
                                                                  tttGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAg                                  <  2:1618920/100‑1 (MQ=255)
                                                                   ttGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGt                                 <  2:1628417/100‑1 (MQ=255)
                                                                    tGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTg                                <  1:2960154/100‑1 (MQ=255)
                                                                     gCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGTATAGCGCGACGAAGGCGAGAAAACAGTGa                               >  1:2931755/1‑100 (MQ=255)
                                                                     gCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGa                               >  2:1147957/1‑100 (MQ=255)
                                                                      cAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAg                              >  1:2601044/1‑100 (MQ=255)
                                                                      cAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAg                              <  1:40565/100‑1 (MQ=255)
                                                                       aGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGc                             <  2:1858066/100‑1 (MQ=255)
                                                                        ggATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCa                            <  2:1129547/100‑1 (MQ=255)
                                                                        ggATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCa                            <  2:3004174/100‑1 (MQ=255)
                                                                         cgttgggTCACCATCGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCaa                           >  1:1358859/7‑100 (MQ=255)
                                                                           tatCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGt                         >  2:1670806/1‑100 (MQ=255)
                                                                             tCGTCACGATCGTGCCCCAACACGATCTTCGTCGCCCCCAGTTCCGTTCAGGTACGATAACCGATAACGCGACGAAGGCGCGAACACCGTGAGCACGTga                       <  1:693486/100‑2 (MQ=255)
                                                                               gTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGCCGAAGGCGAGAACACAGTGAGCAAGTGGtt                     >  2:327770/1‑100 (MQ=255)
                                                                               gTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGtt                     >  2:2100317/1‑100 (MQ=255)
                                                                                tCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGttt                    >  1:1347498/1‑100 (MQ=255)
                                                                                 cACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGtttt                   >  1:1236542/1‑100 (MQ=255)
                                                                                 cACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGtttt                   <  1:1778380/100‑1 (MQ=255)
                                                                                 cACGATGGTGACCCAACACGATCTTCGTCGCCACCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGtttt                   >  2:3281843/1‑100 (MQ=255)
                                                                                  aCGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg                  <  1:210518/100‑1 (MQ=255)
                                                                                  aCGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGCTCCGTGGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg                  <  1:3024589/100‑1 (MQ=255)
                                                                                  aCGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg                  <  1:2812973/100‑1 (MQ=255)
                                                                                    gATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGcc                <  1:3418039/100‑1 (MQ=255)
                                                                                      tGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCct              >  2:1267936/1‑100 (MQ=255)
                                                                                      tGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCct              <  2:2189080/100‑1 (MQ=255)
                                                                                         tGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTg           >  1:1981443/1‑100 (MQ=255)
                                                                                         tGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTg           <  2:2326348/100‑1 (MQ=255)
                                                                                           aCCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGa         >  2:2852368/1‑100 (MQ=255)
                                                                                            cccAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGaa        <  2:607293/100‑1 (MQ=255)
                                                                                             ccAACGCGATCTTCGTCGCCGCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAt       >  1:1565000/1‑100 (MQ=255)
                                                                                             ccAACGAGATCTTCGTCGCCCCCAGTTCCGTTGCGGCACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGaa        >  1:852588/1‑99 (MQ=255)
                                                                                              cAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtc      >  2:540183/1‑100 (MQ=255)
                                                                                              cAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtc      <  2:2122304/100‑1 (MQ=255)
                                                                                              cAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtc      <  1:2877041/100‑1 (MQ=255)
                                                                                                  gcgATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtcttct  >  2:3035375/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
CATCGCTCATCAGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCT  >  NC_000913/1411414‑1411611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: