Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,666,470 C→A 16.4% L386I (CTA→ATA)  clcB → H(+)/Cl(‑) exchange transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,666,4700CA16.4% 317.6 / 43.7 109L386I (CTA→ATA) clcBH(+)/Cl(‑) exchange transporter
Reads supporting (aligned to +/- strand):  ref base C (47/44);  new base A (10/8);  total (57/52)
Fisher's exact test for biased strand distribution p-value = 8.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

TTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGC  >  NC_000913/1666371‑1666568
                                                                                                   |                                                                                                  
ttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGc                                                                                                    <  1:3418891/100‑1 (MQ=255)
 tACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCt                                                                                                   <  2:3309272/100‑1 (MQ=255)
   cTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTAc                                                                                                 <  1:2751780/100‑1 (MQ=255)
      ggATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTcc                                                                                              >  2:144917/1‑100 (MQ=255)
       gATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTccc                                                                                             <  1:2803375/100‑1 (MQ=255)
        aTTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTcccc                                                                                            <  2:1596183/100‑1 (MQ=255)
        aTTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTcccc                                                                                            <  2:2721133/100‑1 (MQ=255)
        aTTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTcccc                                                                                            <  2:140826/100‑1 (MQ=255)
         ttGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCg                                                                                           <  2:2678326/100‑1 (MQ=255)
         ttGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCg                                                                                           <  2:2680101/100‑1 (MQ=255)
          tGACCGGGATGGCGACACTGTTGGCGGCGACCTCGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCgn                                                                                          <  2:1683342/100‑2 (MQ=255)
          tGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCgg                                                                                          <  2:2067777/100‑1 (MQ=255)
          tGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCgg                                                                                          >  2:1024600/1‑100 (MQ=255)
          tGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCgg                                                                                          <  1:2451224/100‑1 (MQ=39)
           gACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGt                                                                                         >  2:2962362/1‑100 (MQ=255)
           gACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGt                                                                                         >  2:1034890/1‑100 (MQ=255)
            aCCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGtt                                                                                        <  2:740523/100‑1 (MQ=255)
            aCCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCGGtc                                                                                        <  1:1254904/100‑2 (MQ=38)
            aCCGGGATGGCGACACTGTCGGCGGCGACCACGCGCGCGCCGCTCATGTCGACGTTGCCGCGAGGGGAAATGACCGGGGAGTATCAGCTACTCCCCGGtt                                                                                        >  1:3226413/1‑100 (MQ=255)
             ccGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGttt                                                                                       >  2:1455532/1‑100 (MQ=255)
               nggATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTat                                                                                     >  2:1119327/2‑100 (MQ=255)
                ggATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTatt                                                                                    <  2:2122504/100‑1 (MQ=255)
                ggATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCGGtcattt                                                                                    <  2:2494372/100‑6 (MQ=37)
                ggATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCGGtca                                                                                       <  1:291662/97‑3 (MQ=38)
                  aTGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTAGCTGATACTCCCCGGtcatttca                                                                                  <  1:1094401/100‑8 (MQ=37)
                   tggtgaatcTGTTCGCGGCGACCATGAACGCGCTGATTATGTCGACGTTGATGATATGTGACATGGCCGGGGAGTATCAGCTACTCCCCGGTTTattgat                                                                                 <  1:2126825/92‑1 (MQ=255)
                      cGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGc                                                                              >  2:487495/1‑100 (MQ=255)
                      cGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGc                                                                              >  2:2007934/1‑100 (MQ=255)
                         cacTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTg                                                                           <  2:112708/100‑1 (MQ=255)
                         cacTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTg                                                                           <  1:1663562/100‑1 (MQ=255)
                           cTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCg                                                                         <  2:1967456/100‑1 (MQ=255)
                           cTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCg                                                                         <  2:1193400/100‑1 (MQ=255)
                           cTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCg                                                                         <  1:1455519/100‑1 (MQ=255)
                            tGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGt                                                                        <  2:302450/100‑1 (MQ=255)
                             gTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTa                                                                       >  1:230063/1‑100 (MQ=255)
                              ttGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTaa                                                                      >  1:2654833/1‑100 (MQ=255)
                                  cggcgACCACGCACGAGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGc                                                                  <  1:960012/100‑1 (MQ=255)
                                    gcgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGt                                                                >  2:1897655/1‑100 (MQ=255)
                                     cgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTc                                                               >  2:338925/1‑100 (MQ=255)
                                       aCCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCgg                                                             <  1:3109206/100‑1 (MQ=255)
                                       aCCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCgg                                                             <  1:600535/100‑1 (MQ=255)
                                         cacgcacgCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTa                                                           >  2:1437296/1‑100 (MQ=255)
                                             cacgcGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAAttt                                                       >  2:3227950/1‑100 (MQ=255)
                                               cgcgCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTc                                                      <  1:1131829/99‑1 (MQ=255)
                                                gcgcCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgc                                                    <  1:3446612/100‑1 (MQ=255)
                                                gcgcCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgc                                                    >  2:3205163/1‑100 (MQ=255)
                                                 cgcCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgcg                                                   >  1:1004140/1‑100 (MQ=255)
                                                 cgcCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgcg                                                   <  1:1473331/100‑1 (MQ=255)
                                                 cgcCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgcg                                                   >  1:1119027/1‑100 (MQ=255)
                                                 cgcCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgcg                                                   <  1:3089768/100‑1 (MQ=255)
                                                    cGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGa                                                 <  2:1441820/99‑1 (MQ=255)
                                                          tGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTaca                                          >  2:2044803/1‑100 (MQ=255)
                                                            tCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACAcc                                        <  1:2120749/100‑1 (MQ=255)
                                                                 gTTGATGATATGTGAAATGACCGGGGAGTATCAGCTGCTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGAc                                   <  1:1809698/100‑1 (MQ=255)
                                                                 gTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGAc                                   <  2:389711/100‑1 (MQ=255)
                                                                   tgatgaTATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGActc                                 >  1:499623/1‑100 (MQ=255)
                                                                   tgatgaTATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATGGCCTGCGTAAGTGCGTCGGTAATTTCGCGGACGTTACACCGTGActc                                 <  1:1332109/100‑1 (MQ=255)
                                                                    gatgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGActct                                >  2:2026435/1‑100 (MQ=255)
                                                                    gatgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGActct                                >  1:1798923/1‑100 (MQ=255)
                                                                     atgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtcta                               <  1:428701/100‑1 (MQ=255)
                                                                      tgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctat                              >  2:1623733/1‑100 (MQ=255)
                                                                      tgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctat                              >  1:3453044/1‑100 (MQ=255)
                                                                       gatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGCCGGTAATTTCGCGGACGTTACACCGTGACtcta                               >  1:2816596/1‑98 (MQ=255)
                                                                        atatGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatct                            <  2:3252007/100‑1 (MQ=255)
                                                                           tgtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTAcc                         >  2:2166257/1‑100 (MQ=255)
                                                                             tgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGc                       >  2:140127/1‑100 (MQ=255)
                                                                              gAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGcc                      <  1:3227926/100‑1 (MQ=255)
                                                                              gAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGcc                      <  2:662752/100‑1 (MQ=255)
                                                                              gAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGcc                      >  2:2233349/1‑100 (MQ=255)
                                                                              caataaaCCGGGGAGTAGCTGATACTCCCCTGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGcc                      >  2:1311037/7‑100 (MQ=37)
                                                                                 aTGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGc                   <  2:2295913/100‑1 (MQ=255)
                                                                                 aTGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAACTTCGCGGACGTTACATCGTGACTCTATCTACCGCCAGc                   <  1:2007917/100‑1 (MQ=255)
                                                                                   aaCCGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGcac                 >  2:3228331/2‑100 (MQ=38)
                                                                                    aCCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGcaca                >  2:562506/1‑100 (MQ=255)
                                                                                     ccGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCacac               >  2:953349/1‑100 (MQ=255)
                                                                                     ccGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCacac               >  1:2005489/1‑100 (MQ=255)
                                                                                     ccGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCacac               >  2:252895/1‑100 (MQ=255)
                                                                                     ccGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCacac               >  1:1510479/1‑100 (MQ=255)
                                                                                     ccGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCacac               >  1:928906/1‑100 (MQ=39)
                                                                                     ccGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCacac               >  1:3449610/1‑100 (MQ=39)
                                                                                     ccGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCacac               >  1:3047928/1‑100 (MQ=39)
                                                                                      cGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTCATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTCTCTACCTCCAGCACACt              >  1:1442931/1‑100 (MQ=255)
                                                                                      cGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACt              >  1:1448505/1‑100 (MQ=255)
                                                                                       ggggAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTg             >  2:719366/1‑100 (MQ=255)
                                                                                       ggggAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTg             >  2:3091305/1‑100 (MQ=255)
                                                                                       ggggAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTg             >  2:3091750/1‑100 (MQ=255)
                                                                                       ggggAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCacac               >  1:2150832/1‑98 (MQ=255)
                                                                                       ggggAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTg             >  2:2079157/1‑100 (MQ=255)
                                                                                          gAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTgcgc          <  2:3102584/100‑1 (MQ=255)
                                                                                           aGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgca         >  2:332611/1‑100 (MQ=255)
                                                                                            tgagcaccTACGCCCCAGTTTATTGATGTCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgcag        <  2:3231356/93‑1 (MQ=255)
                                                                                              aTCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgcagca      >  2:2925895/1‑100 (MQ=255)
                                                                                              aTCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgcagca      >  2:2028545/1‑100 (MQ=255)
                                                                                              aTCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgcagc       >  1:637878/1‑99 (MQ=255)
                                                                                                cAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATa    >  1:1068094/1‑100 (MQ=255)
                                                                                                 aGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAg   >  1:2330119/1‑100 (MQ=255)
                                                                                                 aGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAg   >  2:1511071/1‑100 (MQ=255)
                                                                                                 aGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAg   >  2:641276/1‑100 (MQ=255)
                                                                                                  gCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGc  <  1:2660302/100‑1 (MQ=255)
                                                                                                  gCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGc  <  2:191344/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
TTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGC  >  NC_000913/1666371‑1666568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: