Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 236,569 C→T 40.2% A168V (GCC→GTC)  dnaQ → DNA polymerase III epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913236,5690CT40.2% 106.4 / 161.9 144A168V (GCC→GTC) dnaQDNA polymerase III epsilon subunit
Reads supporting (aligned to +/- strand):  ref base C (41/45);  new base T (32/26);  total (73/71)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 151 total aligned reads displayed.

GGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAAC  >  NC_000913/236470‑236668
                                                                                                   |                                                                                                    
ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGc                                                                                                      <  2:266813/100‑1 (MQ=255)
ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGc                                                                                                      >  2:3081582/1‑100 (MQ=255)
ggAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGc                                                                                                      <  2:3171981/100‑1 (MQ=255)
     aTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGa                                                                                                 >  2:271520/1‑100 (MQ=255)
          tCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCtt                                                                                            >  1:2995071/1‑100 (MQ=255)
           cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTg                                                                                           <  1:3299482/100‑1 (MQ=255)
           cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTg                                                                                           >  1:243207/1‑100 (MQ=255)
            ccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGCTCCCCAGATCCTTGc                                                                                          >  2:2015008/1‑100 (MQ=255)
            ccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGc                                                                                          <  1:561029/100‑1 (MQ=255)
               gTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGa                                                                                       <  2:2608009/100‑1 (MQ=255)
                  aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGt                                                                                    >  2:502659/1‑100 (MQ=255)
                   gcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGtt                                                                                   >  1:848032/1‑100 (MQ=255)
                   gcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGtt                                                                                   <  1:956145/100‑1 (MQ=255)
                     gcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTa                                                                                 >  1:973101/1‑100 (MQ=255)
                     gcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTa                                                                                 <  1:2956284/100‑1 (MQ=255)
                      cAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTAt                                                                                >  1:1172223/1‑100 (MQ=255)
                         cAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTg                                                                             <  1:2026445/100‑1 (MQ=255)
                           gCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGc                                                                          >  1:1010678/1‑100 (MQ=255)
                            ccTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCg                                                                         >  2:3458671/1‑100 (MQ=255)
                            ccTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCg                                                                         >  2:686191/1‑100 (MQ=255)
                             cTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGa                                                                        <  2:271938/100‑1 (MQ=255)
                             cTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGa                                                                        <  2:266588/100‑1 (MQ=255)
                              tCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTCCTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGa                                                                        <  1:1849214/99‑1 (MQ=255)
                               cGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATg                                                                      >  1:1714505/1‑100 (MQ=255)
                                gATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGa                                                                     >  1:3057647/1‑100 (MQ=255)
                                    cGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCgg                                                                 >  2:2369799/1‑100 (MQ=255)
                                     gTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCgct                                                                <  2:3202015/100‑3 (MQ=255)
                                       tATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtgg                                                              <  1:1975521/100‑1 (MQ=255)
                                       tATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtgg                                                              <  1:3299004/100‑1 (MQ=255)
                                         tgtgCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTc                                                            >  1:1613226/1‑100 (MQ=255)
                                         tgtgCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTc                                                            >  2:380005/1‑100 (MQ=255)
                                         tgtgCTCGCTACGAAACAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTc                                                            <  1:2029681/100‑1 (MQ=255)
                                           tgCTCGCTACGAAATAGATAACAGTAAAGGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaa                                                          <  2:653598/100‑1 (MQ=255)
                                           tgCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaa                                                          >  2:249063/1‑100 (MQ=255)
                                           tgCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaa                                                          >  1:3284049/1‑100 (MQ=255)
                                           tgCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaa                                                          >  1:3284469/1‑100 (MQ=255)
                                             cTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAAc                                                        >  1:350518/1‑100 (MQ=255)
                                             cTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAAc                                                        >  2:406598/1‑100 (MQ=255)
                                             cTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAAc                                                        >  2:1776988/1‑100 (MQ=255)
                                             cTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAAc                                                        >  1:1558438/1‑100 (MQ=255)
                                             cTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAAc                                                        <  2:781131/100‑1 (MQ=255)
                                             cTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATGACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAAc                                                        >  2:1998818/1‑100 (MQ=255)
                                              tCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACg                                                       >  1:1175263/1‑100 (MQ=255)
                                              tCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACg                                                       <  2:2623338/100‑1 (MQ=255)
                                              tCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACg                                                       <  1:2753189/100‑1 (MQ=255)
                                               cGCTACGAAATAGATAACAGTAAACGAACGCTGCCCGGGGCATTACTCGATGTCCAGATCGTTGCGGAAGTTTATCTGGCGCTGACCGTTGGCCa                                                           >  1:2639227/1‑95 (MQ=255)
                                                gCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTc                                                     <  1:3147258/100‑1 (MQ=255)
                                                 cTACGAAATAGATAACAGTAAACGAACGCTTCAAGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg                                                    >  1:2268218/1‑100 (MQ=255)
                                                 cTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg                                                    >  2:2618470/1‑100 (MQ=255)
                                                 cTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg                                                    <  2:2258099/100‑1 (MQ=255)
                                                 cTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACCCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg                                                    <  2:2502151/100‑1 (MQ=255)
                                                    cGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATg                                                 >  2:1410051/1‑100 (MQ=255)
                                                         tAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCttt                                            >  2:1235570/1‑100 (MQ=255)
                                                           gATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg                                          <  1:2044039/100‑1 (MQ=255)
                                                            atcaCAGTAAACGAACGCTGCACGGGGCATTACGCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCCAACGTCGATGGCTTTTGc                                         <  2:3434249/97‑1 (MQ=255)
                                                               aCAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGAt                                      >  1:313257/1‑100 (MQ=255)
                                                               aCAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGAt                                      >  1:2609001/1‑100 (MQ=255)
                                                               aCAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGAt                                      <  2:3346704/100‑1 (MQ=255)
                                                                cAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg                                     >  1:3255866/1‑100 (MQ=255)
                                                                 aGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATgg                                    >  1:3048123/1‑100 (MQ=255)
                                                                 aGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATgg                                    <  2:243209/100‑1 (MQ=255)
                                                                    aaaCGAACGCTGCACGGGGCATTACTCGATGTCCAGATACTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAg                                 >  1:2132849/1‑100 (MQ=255)
                                                                     aacgaacgCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAgg                                <  2:2028445/100‑1 (MQ=255)
                                                                      acgaacgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGga                               <  2:2787699/100‑1 (MQ=255)
                                                                      acgaacgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGga                               <  2:2033678/100‑1 (MQ=255)
                                                                       cgaacgCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgag                              <  2:2377039/100‑1 (MQ=255)
                                                                       cgaacgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgag                              >  1:1739872/1‑100 (MQ=255)
                                                                          acgCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgagaga                           >  1:3163867/1‑100 (MQ=255)
                                                                          acgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgagaga                           >  2:42146/1‑100 (MQ=255)
                                                                          acgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgagaga                           >  2:2975987/1‑100 (MQ=255)
                                                                           cgCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGac                          <  2:2958945/100‑1 (MQ=255)
                                                                            gCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGaca                         <  2:835445/100‑1 (MQ=255)
                                                                            gCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGaca                         <  1:247212/100‑1 (MQ=255)
                                                                            gCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGaca                         >  2:2289451/1‑100 (MQ=255)
                                                                            gCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGaca                         >  2:2927727/1‑100 (MQ=255)
                                                                             cTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGacac                        >  1:1287681/1‑100 (MQ=255)
                                                                               gCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaa                      >  2:2752191/1‑100 (MQ=255)
                                                                                cACGGGGCATTACTCGATGTCCAGATCCTTGCGGCAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaac                     <  1:1829213/100‑1 (MQ=255)
                                                                                cACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaac                     <  2:147434/100‑1 (MQ=255)
                                                                                cACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaac                     <  2:168768/100‑1 (MQ=255)
                                                                                 aCGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaaca                    >  2:647249/1‑100 (MQ=255)
                                                                                 aCGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGATTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaaca                    >  2:2437094/1‑100 (MQ=255)
                                                                                  ccgggAATTACTCTGTGTCCAGATCTCTGCTTAAGTTTACTGAGCCAGGACCGGGGGTCAAACGTCGATGTCTTTTACGATATAAGGAGAGACacaaca                    <  1:1433739/97‑1 (MQ=255)
                                                                                  cGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAg                   >  1:707337/1‑100 (MQ=255)
                                                                                  cGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAg                   <  1:2317863/100‑1 (MQ=255)
                                                                                  cGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAg                   <  2:1901400/100‑1 (MQ=255)
                                                                                   ggggCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGc                  >  1:2726300/1‑100 (MQ=255)
                                                                                    gggCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGca                 >  2:1200488/1‑100 (MQ=255)
                                                                                    gggCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGca                 <  2:1845385/100‑1 (MQ=255)
                                                                                      gCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGcaac               <  2:1714521/100‑1 (MQ=255)
                                                                                        aTTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGcaacaa             <  2:1739888/100‑1 (MQ=255)
                                                                                          tACTCGATGTCCAGATCCTTGCGGAAGTTTATCTTGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAAGAAgg           >  1:796250/1‑100 (MQ=255)
                                                                                           aCTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGt          >  1:1980589/1‑100 (MQ=255)
                                                                                           aCTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGt          >  2:1024939/1‑100 (MQ=255)
                                                                                               gATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAg      <  1:502655/100‑1 (MQ=255)
                                                                                               gATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAg      <  2:3268323/100‑1 (MQ=255)
                                                                                                aTGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGc     <  1:604892/100‑1 (MQ=255)
                                                                                                aTGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGc     <  2:2608433/100‑1 (MQ=255)
                                                                                                aTGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGc     <  1:1032883/100‑1 (MQ=255)
                                                                                                   cccAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGcaac  >  2:1505232/1‑100 (MQ=255)
                                                                                                   |                                                                                                    
GGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAAC  >  NC_000913/236470‑236668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: