Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,372,870 T→C 27.6% V105A (GTG→GCG)  arnE → undecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,372,8700TC27.6% 175.6 / 85.9 116V105A (GTG→GCG) arnEundecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit
Reads supporting (aligned to +/- strand):  ref base T (43/41);  new base C (16/16);  total (59/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 119 total aligned reads displayed.

TGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCA  >  NC_000913/2372771‑2372968
                                                                                                   |                                                                                                   
tGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATtgt                                                                                                     <  1:9790/100‑1 (MQ=255)
   aTTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGAt                                                                                                  >  2:3170830/1‑100 (MQ=255)
     tttGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATcc                                                                                                <  1:3174218/100‑1 (MQ=255)
        gTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCg                                                                                             >  2:2197878/1‑100 (MQ=255)
        gTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCg                                                                                             >  2:1531108/1‑100 (MQ=255)
         tCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCgg                                                                                            <  2:3450597/100‑1 (MQ=255)
          cTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCggg                                                                                           <  2:1619849/100‑1 (MQ=255)
          cTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCggg                                                                                           <  2:233326/100‑1 (MQ=255)
          cTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCggg                                                                                           <  2:895910/100‑1 (MQ=255)
          cTGGGTGACGCTGGCTGCAGTAAAACTATGGCACGAAACGGTATCGACGCGACACTGGAGTTGGGTGGCGGTCATTATTGGCGACATTGTGATCATCg                                                                                             >  1:1187746/1‑98 (MQ=255)
            gggTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCCTGGTGATCCTCGGGa                                                                                          <  1:2608878/99‑1 (MQ=255)
            gggTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAg                                                                                         >  2:2367550/1‑100 (MQ=255)
            gggTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAg                                                                                         <  2:318501/100‑1 (MQ=255)
            gggTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAg                                                                                         >  1:2396074/1‑100 (MQ=255)
            gggTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAg                                                                                         >  2:1311597/1‑100 (MQ=255)
            gggTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAg                                                                                         <  1:1613843/100‑1 (MQ=255)
              gTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTa                                                                                       <  2:3305012/100‑1 (MQ=255)
              gTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTa                                                                                       <  2:546725/100‑1 (MQ=255)
                gACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACg                                                                                     >  2:1038292/1‑100 (MQ=255)
                 aCGCTGGCTGCAGTAAAACTGTGGCACGAACCGGCATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACgg                                                                                    >  1:419328/1‑100 (MQ=255)
                  cGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGgt                                                                                   >  1:3209876/1‑100 (MQ=255)
                   gctggctgCAGTAAAACTGTGGCACGAACCGGTATTGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGgtg                                                                                  >  1:3141223/1‑100 (MQ=255)
                   gctggctgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGgtg                                                                                  >  2:678087/1‑100 (MQ=255)
                     tggctgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTa                                                                                <  1:2730987/100‑1 (MQ=255)
                     tggctgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTa                                                                                <  2:2405674/100‑1 (MQ=255)
                      ggctgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGGTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTaa                                                                               >  2:245311/1‑100 (MQ=255)
                        ctgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGgtgtca                                                                               >  2:2071853/1‑96 (MQ=255)
                        ctgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATg                                                                             >  1:1223747/1‑100 (MQ=255)
                         tgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATgg                                                                            >  1:212585/1‑100 (MQ=255)
                          gCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATggg                                                                           >  1:1274456/1‑100 (MQ=255)
                          gCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATggg                                                                           >  1:2413006/1‑100 (MQ=255)
                          gCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATggg                                                                           <  2:1308709/100‑1 (MQ=255)
                           cAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGc                                                                          >  2:2868687/1‑100 (MQ=255)
                           cAGTAAAACTGTAGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGc                                                                          >  2:2276143/1‑100 (MQ=255)
                            aGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCGCTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGcc                                                                         <  1:3419745/100‑1 (MQ=255)
                            aGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGcc                                                                         <  1:385671/100‑1 (MQ=255)
                             gTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCt                                                                        >  2:2318083/1‑100 (MQ=255)
                             gTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCt                                                                        >  2:799745/1‑100 (MQ=255)
                              tAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTg                                                                       <  2:781415/100‑1 (MQ=255)
                               aaaaCTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGa                                                                      >  2:421850/1‑100 (MQ=255)
                                 aaCTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGAtg                                                                   >  1:2653219/1‑100 (MQ=255)
                                   cTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGAtgtg                                                                 <  1:1456756/100‑1 (MQ=255)
                                     gtgGCACGAACCGGTATCGCTGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGAGCCTCGGGAGTACGGTGTAATGGGCCTGATGTggg                                                               <  1:3093605/100‑1 (MQ=255)
                                     gtgGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTggg                                                               <  2:1274468/100‑1 (MQ=255)
                                     gtgGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTggg                                                               <  2:2428431/100‑1 (MQ=255)
                                      tgGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTgggg                                                              >  2:2726466/1‑100 (MQ=255)
                                       ggCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTgggg                                                              >  1:1431683/1‑99 (MQ=255)
                                       ggCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGa                                                             >  2:957164/1‑100 (MQ=255)
                                        gCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGat                                                            <  1:3302262/100‑1 (MQ=255)
                                        gCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGa                                                             >  2:510427/1‑99 (MQ=255)
                                         cACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGatt                                                           >  1:1261501/1‑100 (MQ=255)
                                         cACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGatt                                                           <  1:3170806/100‑1 (MQ=255)
                                         cACGAACAGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTGGATTATTGGAGTCACTGTGATCGTGAGGCGGACGCGTTAATGGGTCTGATAGTgggat                                                             >  1:3189927/1‑97 (MQ=255)
                                             aaCCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTc                                                       <  2:2110558/100‑1 (MQ=255)
                                               ccGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAg                                                     >  2:1865670/1‑100 (MQ=255)
                                                cGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGc                                                    <  2:1997240/100‑1 (MQ=255)
                                                  gTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGt                                                  >  1:2796327/1‑100 (MQ=255)
                                                    aTCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCa                                                <  1:628758/100‑1 (MQ=255)
                                                    aTCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCa                                                <  1:1110178/100‑1 (MQ=255)
                                                     tCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCat                                               >  2:1972733/1‑100 (MQ=255)
                                                      cgccgcGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTGGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCat                                               >  1:1855509/1‑99 (MQ=255)
                                                       gccgcGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCatta                                             >  1:2228054/1‑100 (MQ=255)
                                                       gccgcGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCatta                                             >  2:895484/1‑100 (MQ=255)
                                                       gccgcGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCatta                                             >  2:3020550/1‑100 (MQ=255)
                                                         cgcgTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCattatt                                           <  1:1116938/100‑1 (MQ=255)
                                                          gcgTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTg                                          <  2:2796350/100‑1 (MQ=255)
                                                            gTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGcc                                        <  2:3394326/100‑1 (MQ=255)
                                                            gTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGcc                                        <  1:3006495/100‑1 (MQ=255)
                                                             tCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCt                                       <  1:115567/100‑1 (MQ=255)
                                                                  ggTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGtt                                  <  2:2477605/100‑1 (MQ=255)
                                                                  ggTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGtt                                  >  2:2605867/1‑100 (MQ=255)
                                                                  ggTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGACCTGATGTGTGGATTATTCAGCGTCATTATTGCCTCAGtt                                  >  1:3373170/1‑100 (MQ=255)
                                                                     gtgGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCCGCGTCATTATTGCCTCAGTTgcg                               <  1:1400495/100‑1 (MQ=255)
                                                                     gtgGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTgcg                               <  2:126675/100‑1 (MQ=255)
                                                                      tgGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTgcgc                              <  1:245309/100‑1 (MQ=255)
                                                                        gggTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACAGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCaa                            >  2:1925925/1‑100 (MQ=255)
                                                                         ggTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAAt                           <  1:1982665/100‑1 (MQ=255)
                                                                          gTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAAtt                          <  2:3361873/100‑1 (MQ=255)
                                                                           tGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGCTTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTa                         <  2:2440202/100‑1 (MQ=255)
                                                                             gCGTTCATTATTGGCGGCATTGTGATCCTCGGTATTACGGTGTAATAGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAg                       <  1:2390237/100‑1 (MQ=255)
                                                                             gCGTTCATTATTGGCGGCATTGTGATCCGCGGGAGTACGGTGTAATGGGCCTGATTTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCTATTAAg                       <  1:448462/100‑1 (MQ=255)
                                                                               gTTCATTATTGGCGGCATTGTGATCCCCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGcc                     >  2:2108169/1‑100 (MQ=255)
                                                                               gTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGcc                     >  1:299242/1‑100 (MQ=255)
                                                                                  cATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTgg                  <  1:946046/100‑1 (MQ=255)
                                                                                  cATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTgg                  >  1:863060/1‑100 (MQ=255)
                                                                                  cATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTgg                  >  2:2381861/1‑100 (MQ=255)
                                                                                   attattGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGCTTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTg                   >  1:2603114/1‑98 (MQ=255)
                                                                                   attattGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTggg                 <  2:2846184/100‑1 (MQ=255)
                                                                                   attattGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTggg                 >  2:45208/1‑100 (MQ=255)
                                                                                   attattGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTgg                  <  2:3155097/99‑1 (MQ=255)
                                                                                     tattGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGtt               >  1:2997936/1‑100 (MQ=255)
                                                                                      attGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGttt              >  2:1581213/1‑100 (MQ=255)
                                                                                       ttGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGtttt             <  2:3236343/100‑1 (MQ=255)
                                                                                         ggcggcATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGAATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGtttt             >  1:1812672/1‑98 (MQ=255)
                                                                                           cggcATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgcgg         >  2:3059681/1‑100 (MQ=255)
                                                                                            ggcATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgcggc        >  2:1289756/1‑100 (MQ=255)
                                                                                             gcATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgcggcg       <  1:1531093/100‑1 (MQ=255)
                                                                                             gcATTGCGATCCTCGGGAGTACGGTGTACTGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAACCCTGGGTTTTgcggc        <  1:1021623/99‑1 (MQ=255)
                                                                                               aTTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgcggtg       >  1:2999095/1‑96 (MQ=255)
                                                                                                  gtgATCCTCGGGAGTACGGTGTAGTGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCa  >  2:399810/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGAGTCA  >  NC_000913/2372771‑2372968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: