Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,465,945 G→A 23.8% V215V (GTG→GTA yfdC → putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,465,9450GA23.8% 229.7 / 68.2 117V215V (GTG→GTAyfdCputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (45/44);  new base A (12/16);  total (57/60)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

CCCCCAGCGAGATGTTTGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCA  >  NC_000913/2465848‑2466043
                                                                                                 |                                                                                                  
cccccAGCGAGATGTTTGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGAt                                                                                                  >  2:3199499/1‑100 (MQ=255)
cccccAGCGAGATGTTTGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGAt                                                                                                  <  2:304492/100‑1 (MQ=255)
  cccAGCGAGATGTTTGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATta                                                                                                <  1:1522423/100‑1 (MQ=255)
   ccAGCGAGATGTTTGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATtat                                                                                               >  2:1458919/1‑100 (MQ=255)
    cAGCGAGATGTTTGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATtata                                                                                              <  2:869666/100‑1 (MQ=255)
       cGAGATGTTTGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATtg                                                                                           <  2:3146566/100‑1 (MQ=255)
          gaTGTTTGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATtgatn                                                                                        <  1:475060/100‑2 (MQ=255)
          gaTGTTTGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATtgatg                                                                                        <  1:1041817/100‑1 (MQ=255)
          gaTGTTTGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATtgatg                                                                                        >  2:758845/1‑100 (MQ=255)
          gaTGTTTGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATtgatg                                                                                        <  1:695568/100‑1 (MQ=255)
           aTGTTTGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATtgatg                                                                                        >  2:274557/1‑99 (MQ=255)
               ttGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTg                                                                                   <  2:2313089/100‑1 (MQ=255)
                tGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTgg                                                                                  >  2:3219673/1‑100 (MQ=255)
                 gCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGc                                                                                 >  2:3043212/1‑100 (MQ=255)
                  ccAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCt                                                                                <  2:3186611/100‑1 (MQ=255)
                  ccAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCt                                                                                <  2:1620107/100‑1 (MQ=255)
                  ccAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCt                                                                                >  1:1695725/1‑100 (MQ=255)
                  ccAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCt                                                                                <  1:1122893/100‑1 (MQ=255)
                   cAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCtt                                                                               >  1:3159278/1‑100 (MQ=255)
                     aCGCGATCATGTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCTTAt                                                                             <  1:312584/100‑1 (MQ=255)
                        cgATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGc                                                                          >  2:2669173/1‑100 (MQ=255)
                         gATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGc                                                                          <  1:908292/99‑1 (MQ=255)
                            cATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTg                                                                      <  1:1294257/100‑1 (MQ=255)
                            cATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTg                                                                      <  2:2738180/100‑1 (MQ=255)
                            cATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTg                                                                      <  1:920556/100‑1 (MQ=255)
                             aTTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTgg                                                                     >  2:1405945/1‑100 (MQ=255)
                             aTTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTgg                                                                     <  1:2111813/100‑1 (MQ=255)
                                tCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTg                                                                  <  1:1194646/100‑1 (MQ=255)
                                tCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTg                                                                  <  1:2345410/100‑1 (MQ=255)
                                tCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCTTATTGCCCTGGGTg                                                                  <  2:2576346/100‑1 (MQ=255)
                                tCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCTTATTGCCCTGGGTg                                                                  >  1:1890524/1‑100 (MQ=255)
                                tCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCTTAGTGCCCTggg                                                                    <  1:2965181/98‑1 (MQ=255)
                                  cGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCTTATTGCCCTGGGTGac                                                                >  2:3222745/1‑100 (MQ=255)
                                    gctggctgATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGacac                                                              >  2:2359004/1‑100 (MQ=255)
                                      tggctgATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGacac                                                              <  1:2096582/98‑1 (MQ=255)
                                      tggctgATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGAcacca                                                            >  2:907674/1‑100 (MQ=255)
                                       ngctgATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCACAGATTGTGGAGATTATATTGATAACCTGGCTTATTGCCCTGGGTGAcatcac                                                           >  1:345454/2‑100 (MQ=255)
                                       ggctgATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGAcaccac                                                           >  2:2089857/1‑100 (MQ=255)
                                       ggctgATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGAcaccac                                                           <  2:73311/100‑1 (MQ=255)
                                       ggctgATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGAcacca                                                            >  1:2996559/1‑99 (MQ=255)
                                        gctgATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACaccacc                                                          <  1:3219649/100‑1 (MQ=255)
                                          tgATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCa                                                        <  2:1146681/100‑1 (MQ=255)
                                           gATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCat                                                       >  2:893081/1‑100 (MQ=255)
                                             tCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCatat                                                     >  2:2548678/1‑100 (MQ=255)
                                               gCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCg                                                   <  1:809334/100‑1 (MQ=255)
                                               gCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCg                                                   <  1:809361/100‑1 (MQ=255)
                                               gCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCg                                                   >  1:2819015/1‑100 (MQ=255)
                                               gCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCg                                                   <  1:434483/100‑1 (MQ=255)
                                                ccACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGt                                                  <  2:2911450/100‑1 (MQ=255)
                                                 cACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTg                                                 <  2:2703978/100‑1 (MQ=255)
                                                  aCTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTgg                                                >  2:3171467/1‑100 (MQ=255)
                                                   cTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGt                                               <  2:2042686/100‑1 (MQ=255)
                                                     aTGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCg                                             <  2:3379584/100‑1 (MQ=255)
                                                      tGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCgg                                            <  1:185163/100‑1 (MQ=255)
                                                       ggTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGACGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGt                                           >  1:205247/1‑100 (MQ=255)
                                                         tttGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTc                                         <  2:1794839/100‑1 (MQ=255)
                                                         tttGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTc                                         <  1:860083/100‑1 (MQ=255)
                                                          ttGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCt                                        >  1:2345686/1‑100 (MQ=255)
                                                          ttGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCt                                        >  2:1173316/1‑100 (MQ=255)
                                                            ggATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGt                                      >  2:2416011/1‑100 (MQ=255)
                                                            ggATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGt                                      >  1:2166481/1‑100 (MQ=255)
                                                             gATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGtt                                     >  2:875212/1‑100 (MQ=255)
                                                             gATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGtt                                     <  1:1473595/100‑1 (MQ=255)
                                                              aTGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTg                                    >  2:1890972/1‑100 (MQ=255)
                                                               tGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGa                                   >  2:1112927/1‑100 (MQ=255)
                                                               tGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCAGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGa                                   >  2:2430513/1‑100 (MQ=255)
                                                               tGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGa                                   >  1:1829209/1‑100 (MQ=255)
                                                                gTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGaa                                  >  2:356380/1‑100 (MQ=255)
                                                                gTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGaa                                  >  2:2215745/1‑100 (MQ=255)
                                                                gTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGaa                                  >  2:1774634/1‑100 (MQ=255)
                                                                gTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACATGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGaa                                  >  1:2584262/1‑100 (MQ=255)
                                                                 tttCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGaaa                                 >  2:198814/1‑100 (MQ=255)
                                                                  ttCCTGCAGCGGGTGCGGCAAAGATTGTGGTAATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAAt                                >  2:295519/1‑100 (MQ=255)
                                                                    ccTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAAt                                >  2:532171/1‑98 (MQ=255)
                                                                      tGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCctc                            <  1:487513/100‑1 (MQ=255)
                                                                        cAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGATACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTa                          >  1:1905239/1‑100 (MQ=255)
                                                                           cGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCt                       >  1:2577805/1‑100 (MQ=255)
                                                                             ggTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTgg                     <  2:1879256/100‑1 (MQ=255)
                                                                               tGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGgtg                   <  2:325095/100‑1 (MQ=255)
                                                                                 cGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGtt                 <  1:3068469/100‑1 (MQ=255)
                                                                                 cGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGtt                 <  1:2940559/100‑1 (MQ=255)
                                                                                  ggCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                <  2:3309938/100‑1 (MQ=255)
                                                                                   gCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTa               >  2:1741101/1‑100 (MQ=255)
                                                                                   gCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTa               >  2:1432805/1‑100 (MQ=255)
                                                                                    cAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCGTAACGTGGTTGGTTCTGTTGAAATCCTCTATCTGGTGTTTaa              >  1:2428229/1‑100 (MQ=255)
                                                                                    cAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTaa              >  1:2484341/1‑100 (MQ=255)
                                                                                    cAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTaa              >  2:1887769/1‑100 (MQ=255)
                                                                                    cAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTaa              >  2:383971/1‑100 (MQ=255)
                                                                                    cAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTaa              >  2:1139778/1‑100 (MQ=255)
                                                                                     aaaGATTGTGGTAATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAAc             <  2:1147946/100‑1 (MQ=255)
                                                                                        gATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGt          <  2:3428901/100‑1 (MQ=255)
                                                                                        gATTGTGGTAATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGt          >  1:2540134/1‑100 (MQ=255)
                                                                                         aTTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTa         <  2:2525998/100‑1 (MQ=255)
                                                                                            gtggtgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGc      <  1:758839/100‑1 (MQ=255)
                                                                                            gtggtgATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGc      >  1:1003349/1‑100 (MQ=255)
                                                                                            gtggtaATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGc      >  2:566106/1‑100 (MQ=255)
                                                                                             tggtaATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCt     <  2:1488020/100‑1 (MQ=255)
                                                                                              ggtaATTATTTCGATGACGGGGCTGAGTGGCTTGGGTGAAACGATCCATATCGTGGTATGTTCTGGTGAAATCCTCTATCTGGTGGTTAACgg           <  1:438338/93‑1 (MQ=255)
                                                                                               gtgATTATATTGATGACCTGGCTTATAGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGc   >  2:2105100/1‑100 (MQ=255)
                                                                                                tgATTATATTGATGACCTGGCTTATTGCCCTGGGTGATACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCa  <  2:1905255/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
CCCCCAGCGAGATGTTTGCCAACGCGATCATTTCCGGCTGGCTGATCGCCACTATGGTTTGGATGTTTCCTGCAGCGGGTGCGGCAAAGATTGTGGTGATTATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCA  >  NC_000913/2465848‑2466043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: