Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,764,373 G→A 28.4% H261Y (CAC→TAC)  yfjL ← CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,764,3730GA28.4% 196.3 / 95.5 130H261Y (CAC→TAC) yfjLCP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein
Reads supporting (aligned to +/- strand):  ref base G (51/42);  new base A (17/20);  total (68/62)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

Only 100 of 142 total aligned reads displayed.

GTAGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTCTGGTCGTAAGTAGGA  >  NC_000913/2764275‑2764472
                                                                                                  |                                                                                                   
gTAGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGAtgtgn                                                                                                    <  2:803027/100‑2 (MQ=255)
gTAGCGGTATTTCTTAAGTGAGTACTGCGGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTgg                                                                                                    <  1:2812984/100‑1 (MQ=255)
 tAGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTATGCTTAGCGCAGACCGAAGCCATAGGCGGTCTAAATGGCGTAAGGATGTggg                                                                                                   >  1:522529/1‑100 (MQ=255)
 tAGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTggg                                                                                                   >  2:1339823/1‑100 (MQ=255)
  aGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTgggg                                                                                                  >  2:3088111/1‑100 (MQ=255)
  aGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTgggg                                                                                                  <  1:2067623/100‑1 (MQ=255)
  aGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTaggg                                                                                                  <  2:6909/100‑1 (MQ=255)
   gCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGt                                                                                                 >  2:606265/1‑100 (MQ=255)
    cGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTa                                                                                                >  1:1095432/1‑100 (MQ=255)
      gTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAg                                                                                              >  2:1516129/1‑100 (MQ=255)
      gTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAg                                                                                              <  2:2175229/100‑1 (MQ=255)
      gTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAg                                                                                              <  2:3062331/100‑1 (MQ=255)
       tATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGt                                                                                             >  2:2801805/1‑100 (MQ=255)
       tATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGAGGTGGGGGAAGt                                                                                             >  1:852912/1‑100 (MQ=255)
        ttccgatctgTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTAGGGTAAGTc                                                                                            <  2:3072856/91‑1 (MQ=255)
           tCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCg                                                                                         >  2:1911697/1‑100 (MQ=255)
           tCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCg                                                                                         >  1:3450790/1‑100 (MQ=255)
           tCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCg                                                                                         >  1:2381233/1‑100 (MQ=255)
           tCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCg                                                                                         >  1:2804327/1‑100 (MQ=255)
            cTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTAGGGTAAGTCCCGt                                                                                        >  1:2547484/1‑100 (MQ=255)
             ttAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGAAAGGATGTGGGGTAAGTCCCGtt                                                                                       >  1:13780/1‑100 (MQ=255)
              tAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGttt                                                                                      <  1:1749005/100‑1 (MQ=255)
               aaGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTg                                                                                     >  2:2595723/1‑100 (MQ=255)
               aaGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTAGGGTAAGTCCCGTTTg                                                                                     <  1:1218226/100‑1 (MQ=255)
                aGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTGTAAATGGCGTAAGGATGTAGGGTAAGTCCCGTTTGa                                                                                    <  1:1852731/100‑1 (MQ=255)
                aGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGa                                                                                    <  1:1288706/100‑1 (MQ=255)
                aGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGa                                                                                    <  2:3450819/100‑1 (MQ=255)
                 gTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGAc                                                                                   <  1:2118812/100‑1 (MQ=255)
                 gTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGAc                                                                                   <  1:2226175/100‑1 (MQ=255)
                 gTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTAGGGTAAGTcagatcggaa                                                                                   >  1:3072832/1‑91 (MQ=255)
                      tACTGATGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGa                                                                              <  2:1516983/100‑1 (MQ=255)
                          gctgATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGAtttt                                                                          >  1:2958077/1‑100 (MQ=255)
                          gctgATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGAtttt                                                                          >  2:86943/1‑100 (MQ=255)
                              aTGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCgg                                                                      <  1:3273070/100‑1 (MQ=255)
                               tGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGc                                                                     >  1:2528444/1‑100 (MQ=255)
                               tGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGc                                                                     >  2:49830/1‑100 (MQ=255)
                                gTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGcc                                                                    <  1:1369565/100‑1 (MQ=255)
                                gTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGcc                                                                    <  1:3069762/100‑1 (MQ=255)
                                gTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGcc                                                                    <  1:2657900/100‑1 (MQ=255)
                                gTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGcc                                                                    <  1:1413013/100‑1 (MQ=255)
                                    aaCCGGGCGTTTACGCTTAGCGAAGACCGAAGCGATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCGGTTTGACGTAGATCTTGCGGCCTGAc                                                                <  1:769002/100‑1 (MQ=255)
                                      ccTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCg                                                              <  2:434185/100‑1 (MQ=255)
                                       cTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCga                                                             >  2:2950142/1‑100 (MQ=255)
                                       cTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCga                                                             <  2:526177/100‑1 (MQ=255)
                                          gCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGAt                                                          >  2:3409862/1‑100 (MQ=255)
                                          gCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCGCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGAt                                                          >  2:2434306/1‑100 (MQ=255)
                                           ccTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGAtt                                                         >  2:3131076/1‑100 (MQ=255)
                                           ccTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGAtt                                                         <  2:1648864/100‑1 (MQ=255)
                                             tttACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTgg                                                       >  1:1379329/1‑100 (MQ=255)
                                             tttACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTgg                                                       <  2:2015676/100‑1 (MQ=255)
                                                aCGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGGGATTGGTCa                                                    <  2:13780/100‑1 (MQ=255)
                                                 cGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCa                                                    >  1:881992/1‑99 (MQ=255)
                                                 cGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCAt                                                   >  2:888367/1‑100 (MQ=255)
                                                  gtttAGCGCAGACAGAAGCCATAGGCGCTCTAAATGGCGTAACGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATc                                                  >  2:2340657/3‑100 (MQ=255)
                                                    ttAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCtt                                                >  1:367785/1‑100 (MQ=255)
                                                     tAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTGAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTc                                               >  1:1856043/1‑100 (MQ=255)
                                                     tAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTc                                               <  2:405948/100‑1 (MQ=255)
                                                        ttccgatctaaGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGac                                            <  2:2577841/91‑1 (MQ=255)
                                                        cgcAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGac                                            >  2:1709600/1‑100 (MQ=255)
                                                         gcAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGaca                                           <  1:2892070/100‑1 (MQ=255)
                                                          cggACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGacac                                          >  2:3200690/3‑100 (MQ=255)
                                                          cAGACCGAAGCCAAAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGacac                                          >  2:2350106/1‑100 (MQ=255)
                                                              ccGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCGCGATTGACGTAGATGTTGCGGCGTGACCGAGAATGGTCATCCTCGACACgctg                                      >  1:2986119/1‑100 (MQ=255)
                                                              ccGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACgctg                                      >  2:388043/1‑100 (MQ=255)
                                                               cGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACgctgc                                     <  2:3337696/100‑1 (MQ=255)
                                                                gAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACgctgct                                    <  2:2515737/100‑1 (MQ=255)
                                                                gAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACgctgct                                    >  2:354930/1‑100 (MQ=255)
                                                                 aaGCCATAGGCGCTCTAAATGGCGTAAGGATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCtt                                   <  2:2788746/100‑1 (MQ=255)
                                                                   gCCATAGGCGCTCTAAATGGCGTAAGGCTGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGa                                 >  1:1551628/1‑100 (MQ=255)
                                                                     cATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAAc                               >  1:139124/1‑100 (MQ=255)
                                                                     cATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAAc                               >  2:1582832/1‑100 (MQ=255)
                                                                      aTAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAAcc                              <  2:1312719/100‑1 (MQ=255)
                                                                      aTAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAAcc                              <  1:2896690/100‑1 (MQ=255)
                                                                      aTAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAAcc                              <  1:1058733/100‑1 (MQ=255)
                                                                       tAGGCGCTCTAAATGGCGTAAGGATGGAGGGTAAGTCCCTTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAAc                               <  1:811301/98‑1 (MQ=255)
                                                                         ggCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGGACCACt                           >  1:2851988/1‑100 (MQ=255)
                                                                           cgcTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCa                         >  1:2630706/1‑100 (MQ=255)
                                                                            gcTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCaa                        <  2:3226869/100‑1 (MQ=255)
                                                                              tctAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGt                      >  2:781412/1‑100 (MQ=255)
                                                                                tAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAg                    >  2:542090/1‑100 (MQ=255)
                                                                                 agaTGGCGGAAGGAGGTGGGGTAAGGGCCGTTTGACGTAGAGTTTGCGGCCTGACCGAGATTGGTCAGCTTCGACACGCTGCTTGAACCACTCAAGTAgg                   <  1:3088087/98‑1 (MQ=255)
                                                                                   aTGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTc                 >  1:163619/1‑100 (MQ=255)
                                                                                   aTGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTc                 <  2:2286/100‑1 (MQ=255)
                                                                                    tGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTCt                >  1:491037/1‑100 (MQ=255)
                                                                                     ggCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTCTg               <  2:487596/100‑1 (MQ=255)
                                                                                       catAAGGATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTCTGGt             >  2:2329376/3‑100 (MQ=255)
                                                                                       cGTAAGGATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTCTGGt             >  2:1755689/1‑100 (MQ=255)
                                                                                        gTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTCTGGTc            >  2:596547/1‑100 (MQ=255)
                                                                                         tAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACAGGCTGCTTGAACCACTCATGTAGGTCTGGttg           <  2:2330162/100‑3 (MQ=255)
                                                                                           aGGATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTCTGGTCGTa         <  2:3257596/100‑1 (MQ=255)
                                                                                           aGGATGTAGGGTAAGTCCCGATTGACGTAGATCTTGCGGCCTGACCGAGATTGGTCACCTTCGACACGCTGCTTCAACCACACAAGCAGCTCTGGTc            >  1:967488/1‑97 (MQ=255)
                                                                                            ggATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATCGGTGATCTTTGACACGCTGGTTGAACTACTCAAAGAGCTCTGGTCGt          >  1:370196/1‑98 (MQ=255)
                                                                                             gATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACTCGCTGCTTGAACCACTCAAGTAGGTCTGGTCGTAAg       <  2:2436317/100‑1 (MQ=255)
                                                                                             gATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGCTCTGGTCGTaa        >  1:2137099/1‑99 (MQ=255)
                                                                                             gATGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTCTGGTCGTAAg       >  2:2204205/1‑100 (MQ=255)
                                                                                              aTGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTCTGGTCGTAAGt      >  1:3219864/1‑100 (MQ=255)
                                                                                              aTGTAGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTCTGGTCGTAAGt      <  2:1006418/100‑1 (MQ=255)
                                                                                               tgtaGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTCTGGTCGTAAGTa     >  2:1613054/1‑100 (MQ=255)
                                                                                                 tgGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTCTGGTCGTAAGTAgg   >  1:940554/1‑100 (MQ=255)
                                                                                                  ggggTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTCTGGTCGTAAGTAGGa  >  1:1134430/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
GTAGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGCAGACCGAAGCCATAGGCGCTCTAAATGGCGTAAGGATGTGGGGTAAGTCCCGTTTGACGTAGATTTTGCGGCCTGACCGAGATTGGTCATCTTCGACACGCTGCTTGAACCACTCAAGTAGGTCTGGTCGTAAGTAGGA  >  NC_000913/2764275‑2764472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: