Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,790,586 T→C 56.2% L202P (CTG→CCG)  lhgO → L‑2‑hydroxyglutarate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,790,5860TC56.2% 65.3 / 158.7 130L202P (CTG→CCG) lhgOL‑2‑hydroxyglutarate oxidase
Reads supporting (aligned to +/- strand):  ref base T (36/21);  new base C (38/35);  total (74/56)
Fisher's exact test for biased strand distribution p-value = 2.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

GAAGTCAGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCATAA  >  NC_000913/2790489‑2790682
                                                                                                 |                                                                                                
 aaGTCAGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGAt                                                                                               >  2:48552/1‑100 (MQ=255)
     cAGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCt                                                                                           <  2:2082124/100‑1 (MQ=255)
        cGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGAc                                                                                        <  2:905023/100‑1 (MQ=255)
          ggCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGCATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGAc                                                                                        >  2:3134855/1‑98 (MQ=255)
          ggCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCg                                                                                      >  2:377302/1‑100 (MQ=255)
          ggCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCg                                                                                      >  2:3366008/1‑100 (MQ=255)
              tAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTg                                                                                  >  1:2072386/1‑100 (MQ=255)
               aaTGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTgg                                                                                 <  1:1071391/100‑1 (MQ=255)
                aTGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGt                                                                                <  1:2845322/100‑1 (MQ=255)
                 tGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGCTTAGCTGTTACGGGCTGATGGCTGACCGGCTGGTg                                                                               >  1:1096752/1‑100 (MQ=255)
                 tGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTg                                                                               >  2:2537424/1‑100 (MQ=255)
                 tGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTg                                                                               <  1:2099124/100‑1 (MQ=255)
                 tGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTg                                                                               <  2:1738650/100‑1 (MQ=255)
                 tGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTg                                                                               >  2:1156517/1‑100 (MQ=255)
                  gAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGa                                                                              <  2:915351/100‑1 (MQ=255)
                      aTAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAACAt                                                                          >  1:2177891/1‑100 (MQ=255)
                      aTAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAAt                                                                          >  2:1168366/1‑100 (MQ=255)
                        aaaaaCGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAGGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGaaaa                                                                           <  1:2125324/97‑1 (MQ=255)
                         aaaaCGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCt                                                                       >  2:1795674/1‑100 (MQ=255)
                         aaaaCGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCt                                                                       >  1:948266/1‑100 (MQ=255)
                           aaCGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCg                                                                     <  1:494849/100‑1 (MQ=255)
                           aaCGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCg                                                                     <  2:493490/100‑1 (MQ=255)
                           aaCGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCg                                                                     <  2:1694154/100‑1 (MQ=255)
                            aCGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCgg                                                                    <  2:191218/100‑1 (MQ=255)
                               gCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACt                                                                 <  1:1156506/100‑1 (MQ=255)
                                 gtggtgATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCg                                                               <  1:3319293/100‑1 (MQ=255)
                                 gtggtgATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCg                                                               <  2:2565900/100‑1 (MQ=255)
                                    gtgATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAAc                                                            >  2:3039177/1‑100 (MQ=255)
                                     tgATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAAcc                                                           >  2:1554349/1‑100 (MQ=255)
                                     tgATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAAcc                                                           >  1:2591008/1‑100 (MQ=255)
                                      gATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCg                                                          >  1:2943340/1‑100 (MQ=255)
                                        tACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCggg                                                        >  1:714312/1‑100 (MQ=255)
                                          cGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCt                                                      >  1:395614/1‑100 (MQ=255)
                                          cGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCt                                                      >  2:3416920/1‑100 (MQ=255)
                                          cGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCCAACCGTGc                                                       >  2:135531/1‑99 (MQ=255)
                                           gTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCCCGGACTCGAACCGGGCtt                                                     <  2:549368/100‑1 (MQ=255)
                                             aCCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTa                                                   <  2:3226230/100‑1 (MQ=255)
                                             aCCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTa                                                   <  1:546196/100‑1 (MQ=255)
                                              cccGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTat                                                  <  1:1238014/100‑1 (MQ=255)
                                              cccGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTGCCGGGCTGATGGCGGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTat                                                  >  1:2887119/1‑100 (MQ=255)
                                                cGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTatca                                                <  2:1927272/100‑1 (MQ=255)
                                                cGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTatca                                                <  2:3167263/100‑1 (MQ=255)
                                                cGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTatca                                                <  2:3167065/100‑1 (MQ=255)
                                                  tCAGGGCGGCGAATATGAAGCATCAACGCTGATTCGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGGCTCGAACCGGGCTTTatcatc                                              <  2:3262353/100‑1 (MQ=255)
                                                  tCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTatcatc                                              <  2:326676/100‑1 (MQ=255)
                                                  tCAGGGCGGCGAATACGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTatcatc                                              <  2:3410459/100‑1 (MQ=255)
                                                    aGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTg                                            <  1:3039623/100‑1 (MQ=255)
                                                     gggCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGc                                           <  1:1667741/100‑1 (MQ=255)
                                                       gcggcgAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGccc                                         >  2:2748300/1‑100 (MQ=255)
                                                       gcggcgAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGccc                                         >  1:1740004/1‑100 (MQ=255)
                                                         ggcgAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGt                                       >  1:2870731/1‑100 (MQ=255)
                                                          gcgAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGt                                       <  2:2366494/99‑1 (MQ=255)
                                                           cgAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTc                                     <  2:714316/100‑1 (MQ=255)
                                                              atatGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGt                                  <  1:1519383/100‑1 (MQ=255)
                                                               tatGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTg                                 <  2:414501/100‑1 (MQ=255)
                                                               tatGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTg                                 <  1:452153/100‑1 (MQ=255)
                                                               tatGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTg                                 <  1:1881113/100‑1 (MQ=255)
                                                                 tGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGc                               >  1:2755465/1‑100 (MQ=255)
                                                                  gAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCg                              >  2:347349/1‑100 (MQ=255)
                                                                  gAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCg                              >  1:247260/1‑100 (MQ=255)
                                                                  gAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCg                              >  2:1008322/1‑100 (MQ=255)
                                                                  gAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCg                              >  2:3456070/1‑100 (MQ=255)
                                                                  gAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCg                              >  2:2901008/1‑100 (MQ=255)
                                                                    aGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAg                            <  2:2938705/100‑1 (MQ=255)
                                                                    aGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAg                            >  2:1570365/1‑100 (MQ=255)
                                                                    aGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCCCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAg                            >  1:78018/1‑100 (MQ=255)
                                                                     acATCAACGCTGAATAGCTGTTCCGGTCCGATGTCTGACAGGCTGGTGAAACTGCTCAGCCTCGAACCGGGCTTTCTCATCTGCCCCTCCCTTGGGGAg                            >  1:1448120/2‑99 (MQ=255)
                                                                      cATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTa                          >  2:2855182/1‑100 (MQ=255)
                                                                       aTCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTAt                         >  1:2194443/1‑100 (MQ=255)
                                                                       aTCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTAt                         >  2:3245504/1‑100 (MQ=255)
                                                                       aTCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTAt                         >  1:325156/1‑100 (MQ=255)
                                                                         cAACGCTGATTAGCTGTGCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTAttt                       <  2:2500731/100‑1 (MQ=255)
                                                                           aCGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTcc                     >  2:1733279/1‑100 (MQ=255)
                                                                            cGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCg                    <  2:1718727/100‑1 (MQ=255)
                                                                            cGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCg                    >  2:1941457/1‑100 (MQ=255)
                                                                              cTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGcc                  <  1:2952406/100‑1 (MQ=255)
                                                                              cTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGcc                  <  1:3098681/100‑1 (MQ=255)
                                                                              cTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGc                   <  2:2368108/99‑1 (MQ=255)
                                                                               tGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCt                 <  1:3221004/100‑1 (MQ=255)
                                                                                  ttAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTgc              <  1:3416894/100‑1 (MQ=255)
                                                                                   tAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTgcg             <  2:1081771/100‑1 (MQ=255)
                                                                                    aGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTgcgc            >  1:1497117/1‑100 (MQ=255)
                                                                                    aGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTgcgc            >  1:1262791/1‑100 (MQ=255)
                                                                                     gCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGcccc           >  1:1194582/1‑100 (MQ=255)
                                                                                     gCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCATTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGcc           >  1:2219107/1‑100 (MQ=255)
                                                                                      cTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCg          >  2:2579018/1‑100 (MQ=255)
                                                                                      cTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCg          >  2:929056/1‑100 (MQ=255)
                                                                                       tGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCgg         >  2:1889867/1‑100 (MQ=255)
                                                                                       tGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCgg         >  2:1889910/1‑100 (MQ=255)
                                                                                       tGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCgg         >  2:657015/1‑100 (MQ=255)
                                                                                        gTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGa        >  1:2487604/1‑100 (MQ=255)
                                                                                        gTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGa        <  2:1944103/100‑1 (MQ=255)
                                                                                        gTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGa        >  1:1154808/1‑100 (MQ=255)
                                                                                         ttCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAg       >  2:2773373/1‑100 (MQ=255)
                                                                                          tCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGc      >  2:742993/1‑100 (MQ=255)
                                                                                           ccGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCa     >  1:3220724/1‑100 (MQ=255)
                                                                                           ccGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCa     >  2:2679227/1‑100 (MQ=255)
                                                                                            cGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCAt    >  1:3325458/1‑100 (MQ=255)
                                                                                             gggCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCATa   >  1:1715080/1‑100 (MQ=255)
                                                                                               gCTGATGGATGACGGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCTGCCTTGCGCCGGAGCATa   >  1:543267/1‑98 (MQ=255)
                                                                                                 |                                                                                                
GAAGTCAGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCATAA  >  NC_000913/2790489‑2790682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: