BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,182,712	G→A	40.0%	A55T (GCC→ACC)	zupT →	zinc transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,182,712	0	G	A	40.0%	112.0 / 187.7	165	A55T (GCC→ACC)	zupT	zinc transporter
Reads supporting (aligned to +/- strand): ref base G (39/60); new base A (38/28); total (77/88)
Fisher's exact test for biased strand distribution p-value = 2.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01

Only 100 of 168 total aligned reads displayed.

GGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATG  >  NC_000913/3182613‑3182811

ggCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTg                                                                                                     <  1:3122408/100‑1 (MQ=255)

ggCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTg                                                                                                     >  1:2472442/1‑100 (MQ=255)

 gCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGc                                                                                                    <  2:3432607/100‑1 (MQ=255)

   gTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCg                                                                                                  >  1:1465445/1‑100 (MQ=255)

   gTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCg                                                                                                  >  2:1189462/1‑100 (MQ=255)

    ttCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGc                                                                                                 <  2:3004587/100‑1 (MQ=255)

     tctcGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCa                                                                                                >  1:3070195/1‑100 (MQ=255)

      ctcGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCAc                                                                                               >  1:2037926/1‑100 (MQ=255)

        cGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTa                                                                                             >  2:2486111/1‑100 (MQ=255)

        cGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTa                                                                                             >  2:64683/1‑100 (MQ=255)

         gggCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTag                                                                                            >  2:1327052/1‑100 (MQ=255)

          ggCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTagc                                                                                           >  2:394473/1‑100 (MQ=255)

             aaaaaCCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTagcagc                                                                                        <  2:61762/100‑1 (MQ=255)

             aaaaaCCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTagcag                                                                                         >  1:3275785/1‑99 (MQ=255)

              aaagCCCTCGAACCGGTTACTGGCGTTTTCGCTAGGTTTTGCGGCTGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCt                                                                                       <  1:346012/100‑1 (MQ=255)

              aaaaCCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCt                                                                                       <  1:2487312/100‑1 (MQ=255)

              aaaaCCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCt                                                                                       >  1:2753209/1‑100 (MQ=255)

              aaaaCCCTCGAACCGCTTACTGGCGTGTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCt                                                                                       <  2:3268324/100‑1 (MQ=255)

               aaaCCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTg                                                                                      >  1:895744/1‑100 (MQ=255)

                aaCCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGa                                                                                     <  2:468178/100‑1 (MQ=255)

                  cccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAg                                                                                   >  1:1002962/1‑100 (MQ=255)

                   ccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAgg                                                                                  <  1:3202066/100‑1 (MQ=255)

                   ccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAgg                                                                                  >  1:2872301/1‑100 (MQ=255)

                   ccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAgg                                                                                  <  2:710944/100‑1 (MQ=255)

                    cTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGa                                                                                 <  2:41901/100‑1 (MQ=255)

                       gatccGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATg                                                                              <  1:3151254/97‑1 (MQ=255)

                       gAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATg                                                                              <  2:2599625/100‑1 (MQ=255)

                        aaCCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGt                                                                             <  1:1739276/100‑1 (MQ=255)

                          ccGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCg                                                                           >  2:2574362/1‑100 (MQ=255)

                           cGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGc                                                                          >  2:3161505/1‑100 (MQ=255)

                            gCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGTATCATGTTGCTCATCTCATTAATTGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGcc                                                                         <  1:3297727/100‑1 (MQ=255)

                            gCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGcc                                                                         >  2:271597/1‑100 (MQ=255)

                            gCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGcc                                                                         <  2:2200593/100‑1 (MQ=255)

                                  tGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTTATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTgtgt                                                                    >  1:1930780/1‑99 (MQ=255)

                                     cGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTggg                                                                <  1:1232096/100‑1 (MQ=255)

                                          tCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATg                                                           <  2:1863583/100‑1 (MQ=255)

                                           cGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATgg                                                          <  1:2467805/100‑1 (MQ=255)

                                           cGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATgg                                                          <  2:3041705/100‑1 (MQ=255)

                                           cGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATgg                                                          >  2:1087235/1‑100 (MQ=255)

                                           cGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATgg                                                          <  2:1892755/100‑1 (MQ=255)

                                                ggTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGt                                                     <  2:1731926/100‑1 (MQ=255)

                                                ggTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGt                                                     >  1:3218387/1‑100 (MQ=255)

                                                 gTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGtt                                                    <  1:2405307/100‑1 (MQ=255)

                                                 gTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGtt                                                    <  2:1442622/100‑1 (MQ=255)

                                                    ttGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTAt                                                 <  2:2753231/100‑1 (MQ=255)

                                                     tGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATc                                                >  1:514432/1‑100 (MQ=255)

                                                      tccgatctgATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCt                                               <  1:1666286/92‑1 (MQ=255)

                                                      gcggcgGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCt                                               >  2:2675229/1‑100 (MQ=255)

                                                       cggcggGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCtt                                              <  1:3363458/100‑1 (MQ=255)

                                                        ggcggGGATCATGTTGCTCGTCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTc                                             <  2:2941472/100‑1 (MQ=255)

                                                         gcggGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAAGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCg                                            <  2:937502/100‑1 (MQ=255)

                                                          cggGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCgg                                           <  2:1145843/100‑1 (MQ=255)

                                                            gggATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATTTTCGGTc                                         <  2:1818511/100‑1 (MQ=255)

                                                            gggATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTc                                         >  1:2798437/1‑100 (MQ=255)

                                                              gATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCtagatcgga                                       >  2:1666302/1‑92 (MQ=255)

                                                              gATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTc                                             <  1:2337996/94‑1 (MQ=255)

                                                                tCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCt                                     <  2:3304345/100‑1 (MQ=255)

                                                                  atcttGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTg                                   <  2:324228/97‑1 (MQ=255)

                                                                  aTGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTg                                   <  1:3332330/100‑1 (MQ=255)

                                                                   tGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTgg                                  >  1:1444124/1‑100 (MQ=255)

                                                                   tGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTgg                                  >  1:2580152/1‑100 (MQ=255)

                                                                    gTTGCTCATCTCATTAATGGAAATGCTGCCTACCGCACTAGCAGCGGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGc                                 <  1:846461/100‑1 (MQ=255)

                                                                      tGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTa                               >  2:2538106/1‑100 (MQ=255)

                                                                        ctcatctcatTAGTGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTAtt                             >  2:2266148/1‑100 (MQ=255)

                                                                        ctcatctcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTAtt                             >  2:2893505/1‑100 (MQ=255)

                                                                          catctcatTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTAtttt                           >  2:799664/1‑100 (MQ=255)

                                                                            tctcatTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTgg                         <  1:2727976/100‑1 (MQ=255)

                                                                             ctcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGc                        <  2:2477093/100‑1 (MQ=255)

                                                                              tcatTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGcc                       <  1:2872147/100‑1 (MQ=255)

                                                                              tcatTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGcc                       >  1:1225290/1‑100 (MQ=255)

                                                                              tcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGcc                       <  1:1974544/100‑1 (MQ=255)

                                                                              tcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGcc                       >  1:805525/1‑100 (MQ=255)

                                                                              tcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGCCTGCTTGGCTATTTTGGcc                       <  1:3202792/100‑1 (MQ=255)

                                                                               catTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCt                      >  2:1715300/1‑100 (MQ=255)

                                                                                atTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTg                     <  2:3376417/100‑1 (MQ=255)

                                                                                atTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTg                     <  2:514436/100‑1 (MQ=255)

                                                                                 ttAATGGAAATGCTTCCTGCCGCATTAGCAGCTGAAGGAATGTCGCCTGTGTTGGTTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTgg                    <  1:787598/100‑1 (MQ=255)

                                                                                 ttAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTgg                    >  1:2313711/1‑100 (MQ=255)

                                                                                  tAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGa                   >  2:931194/1‑100 (MQ=255)

                                                                                  tAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGa                   >  1:2378853/1‑100 (MQ=255)

                                                                                   aaTGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGAc                  >  1:1821117/1‑100 (MQ=255)

                                                                                   aaTGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGAc                  <  1:2349437/100‑1 (MQ=255)

                                                                                    aTGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGAcc                 <  1:1019587/100‑1 (MQ=255)

                                                                                    aTGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGAcc                 <  1:1415478/100‑1 (MQ=255)

                                                                                     tGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCg                >  1:1149744/1‑100 (MQ=255)

                                                                                      ggAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGc               >  1:401996/1‑100 (MQ=255)

                                                                                      ggAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGc               <  1:303795/100‑1 (MQ=255)

                                                                                       gAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCa              >  1:310253/1‑100 (MQ=255)

                                                                                        aaaTGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGTCCTGGACCGCAt             <  2:2275272/100‑1 (MQ=255)

                                                                                        aaaTGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCAt             <  2:763501/100‑1 (MQ=255)

                                                                                        aaaTGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCAt             <  2:1073628/100‑1 (MQ=255)

                                                                                            gCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTg         <  1:1327040/100‑1 (MQ=255)

                                                                                             cTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGc        >  1:3135949/1‑100 (MQ=255)

                                                                                             cTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGc        >  1:2261187/1‑100 (MQ=255)

                                                                                              ttCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGcc       >  2:2370526/1‑100 (MQ=255)

                                                                                               tCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCca      >  1:2955336/1‑100 (MQ=255)

                                                                                               tCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCca      >  1:407116/1‑100 (MQ=255)

                                                                                                ccTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCcac     <  1:592287/100‑1 (MQ=255)

                                                                                                 cTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCcaca    <  1:3402141/100‑1 (MQ=255)

                                                                                                  tGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACAt   >  2:1505957/1‑100 (MQ=255)

GGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATG  >  NC_000913/3182613‑3182811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`