Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,520,513 T→C 58.4% D64G (GAT→GGT)  hofQ ← DNA catabolic putative fimbrial transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,520,5130TC58.4% 116.3 / 203.8 173D64G (GAT→GGT) hofQDNA catabolic putative fimbrial transporter
Reads supporting (aligned to +/- strand):  ref base T (31/41);  new base C (48/53);  total (79/94)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

TGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAA  >  NC_000913/3520414‑3520612
                                                                                                   |                                                                                                   
tGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACAt                                                                                                     >  1:2043626/1‑100 (MQ=255)
   cAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTg                                                                                                  <  1:2322948/100‑1 (MQ=255)
    aGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGt                                                                                                 >  1:499548/1‑100 (MQ=255)
      gCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTa                                                                                               >  1:807746/1‑100 (MQ=255)
       cAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAg                                                                                              <  2:513614/100‑1 (MQ=255)
       cAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAg                                                                                              >  1:1964578/1‑100 (MQ=255)
         aTGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGAt                                                                                            <  1:1718657/100‑1 (MQ=255)
           ggAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGt                                                                                          <  2:516386/100‑1 (MQ=255)
           ggAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGt                                                                                          <  1:3009799/100‑1 (MQ=255)
             aaTGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTaa                                                                                        >  2:266974/1‑100 (MQ=255)
                 cACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGac                                                                                    >  2:3386182/1‑100 (MQ=255)
                   cTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGacac                                                                                  >  1:149375/1‑100 (MQ=255)
                     gagagaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCg                                                                                <  2:973179/100‑1 (MQ=255)
                     gagagaAGGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCg                                                                                >  2:1826087/1‑100 (MQ=255)
                           aTGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGc                                                                          <  1:1361892/100‑1 (MQ=255)
                           aTGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGc                                                                          >  2:742974/1‑100 (MQ=255)
                             gTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTg                                                                        <  1:119783/100‑1 (MQ=255)
                             gTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTg                                                                        >  1:2247241/1‑100 (MQ=255)
                             gTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTg                                                                        >  1:3069970/1‑100 (MQ=255)
                              ttGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGa                                                                       <  1:34485/100‑1 (MQ=255)
                              ttGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGa                                                                       >  2:2176079/1‑100 (MQ=255)
                               tGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGAc                                                                      <  1:30904/100‑1 (MQ=255)
                                gCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACg                                                                     >  2:886267/1‑100 (MQ=255)
                                 ccTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGt                                                                    >  1:3205739/1‑100 (MQ=255)
                                  cTTCCTGCTGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGTGAACACCTGTTAGATGTAACGTTACCGTACCGCTGACg                                                                     <  1:2131237/98‑1 (MQ=255)
                                    tCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTg                                                                 <  1:1674934/100‑1 (MQ=255)
                                    nccTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTg                                                                 >  2:1237235/2‑100 (MQ=255)
                                     ccTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTgg                                                                <  1:514635/100‑1 (MQ=255)
                                      cTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGc                                                               >  2:2103597/1‑100 (MQ=255)
                                       tGCCGCGTTATCAGTCCGGCGCTTTTCACTACAATTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATATAACGACACCGTACCGCTGACGTCTGGCg                                                              >  1:2075654/1‑100 (MQ=255)
                                         ccGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGac                                                            >  1:1792695/1‑100 (MQ=255)
                                          ngcgTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACCCCGTACCGCTGACGTCTGGCGaca                                                           >  2:1579701/2‑100 (MQ=255)
                                          cgcgTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGaca                                                           >  1:2679601/1‑100 (MQ=255)
                                          cgcgTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGaca                                                           <  2:871818/100‑1 (MQ=255)
                                          cgcgTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGaca                                                           <  2:2268092/100‑1 (MQ=255)
                                               tATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGAcc                                                      >  2:2617922/1‑100 (MQ=255)
                                                aTCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCa                                                     <  2:292801/100‑1 (MQ=255)
                                                aTCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCCGACGTCTGGCGACACGACCa                                                     <  2:3250315/100‑1 (MQ=255)
                                                 tCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAg                                                    >  1:1594451/1‑100 (MQ=255)
                                                  cAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAgg                                                   >  2:3142894/1‑100 (MQ=255)
                                                  cAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAgg                                                   <  1:2899398/100‑1 (MQ=255)
                                                  cAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAgg                                                   >  2:846690/1‑100 (MQ=255)
                                                    gTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGtt                                                 >  1:2857748/1‑100 (MQ=255)
                                                     tCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTc                                                >  2:1838469/1‑100 (MQ=255)
                                                     tCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTc                                                <  1:2103580/100‑1 (MQ=255)
                                                      ccGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTACCGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCa                                               >  1:1649759/1‑100 (MQ=255)
                                                        ggCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAAc                                             <  2:2057683/100‑1 (MQ=255)
                                                        ggCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAAc                                             <  1:3142981/100‑1 (MQ=255)
                                                           gcTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTtc                                          <  1:980983/100‑1 (MQ=255)
                                                             ttttCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTtctc                                        >  2:1658602/1‑100 (MQ=255)
                                                              tttCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTcc                                       <  2:786348/100‑1 (MQ=255)
                                                              tttCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTcc                                       <  2:1540433/100‑1 (MQ=255)
                                                              tttCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTcc                                       >  1:146483/1‑100 (MQ=255)
                                                                tCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTg                                     <  1:615975/100‑1 (MQ=255)
                                                                 cACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGt                                    <  2:521040/100‑1 (MQ=255)
                                                                 cACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGt                                    >  1:1538659/1‑100 (MQ=255)
                                                                   cTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTc                                  <  2:2826400/100‑1 (MQ=255)
                                                                   cTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTAGCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTc                                  >  1:2211260/1‑100 (MQ=255)
                                                                   cTACAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTc                                  >  1:80909/1‑100 (MQ=255)
                                                                     aCAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcag                                <  2:2003654/100‑1 (MQ=255)
                                                                     aCAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcag                                >  1:1410633/1‑100 (MQ=255)
                                                                     aCAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcag                                <  1:1032778/100‑1 (MQ=255)
                                                                     aCAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcag                                >  2:902437/1‑100 (MQ=255)
                                                                      cAGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagc                               <  1:2671968/100‑1 (MQ=255)
                                                                       aGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagcc                              <  2:584452/100‑1 (MQ=255)
                                                                       aGTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagcc                              <  1:2021471/100‑1 (MQ=255)
                                                                        gTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagcca                             <  1:888287/100‑1 (MQ=255)
                                                                        gTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagcca                             >  2:427701/1‑100 (MQ=255)
                                                                        gTTTGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagcca                             >  1:666733/1‑100 (MQ=255)
                                                                          ttGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagccagc                           >  1:2607284/1‑100 (MQ=255)
                                                                          ttGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagccagc                           >  2:228371/1‑100 (MQ=255)
                                                                           tGTAGTGCCTGCTTCCGGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAgcg                          >  2:3044121/1‑100 (MQ=255)
                                                                           tGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAgcg                          >  1:1819740/1‑100 (MQ=255)
                                                                           tGTAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAgcg                          >  2:2388298/1‑100 (MQ=255)
                                                                             tAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGcc                        <  1:938456/100‑1 (MQ=255)
                                                                             tAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGCCACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCATCCAgcgc                         >  1:3369087/1‑99 (MQ=255)
                                                                             tAGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGcc                        <  2:2147321/100‑1 (MQ=255)
                                                                              aGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAgcg                          <  1:1236009/97‑1 (MQ=255)
                                                                              aGTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCt                       >  2:2986388/1‑100 (MQ=255)
                                                                               gTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTg                      <  2:1299956/100‑1 (MQ=255)
                                                                               gTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTg                      >  1:357855/1‑100 (MQ=255)
                                                                               gTGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTg                      >  2:2747288/1‑100 (MQ=255)
                                                                                tGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGc                     <  2:1395769/100‑1 (MQ=255)
                                                                                tGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGc                     <  1:275782/100‑1 (MQ=255)
                                                                                tGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGc                     <  2:1792711/100‑1 (MQ=255)
                                                                                tGCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGc                     <  1:3392787/100‑1 (MQ=255)
                                                                                 gCCTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCa                    >  1:2535107/1‑100 (MQ=255)
                                                                                   cTGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAac                  <  2:1048908/100‑1 (MQ=255)
                                                                                    tGCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAaca                 >  2:1465785/1‑100 (MQ=255)
                                                                                     gCTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAacac                >  2:1441786/1‑100 (MQ=255)
                                                                                      cTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACAcc               >  1:2833914/1‑100 (MQ=255)
                                                                                      cTTCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACAcc               <  2:1591149/100‑1 (MQ=255)
                                                                                        tCCAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTg             <  2:1941130/100‑1 (MQ=255)
                                                                                          naGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAg           >  2:2047424/2‑100 (MQ=255)
                                                                                          cAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAg           >  2:878606/1‑100 (MQ=255)
                                                                                          cAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAg           <  2:2791657/100‑1 (MQ=255)
                                                                                          cAGGGAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAg           >  1:1976918/1‑100 (MQ=255)
                                                                                            gggAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCt         >  1:1118647/1‑100 (MQ=255)
                                                                                             ggAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAg           <  1:1343876/97‑1 (MQ=255)
                                                                                              gAACACCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTAc       <  1:2810286/100‑1 (MQ=255)
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TGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAA  >  NC_000913/3520414‑3520612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: