Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 220,849 (T)5→4 coding (80/816 nt) metQ ← DL‑methionine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913220,8450T.97.7% 378.7 / 2.2 85coding (84/816 nt)metQDL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base . (48/35);  total (49/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

CGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCCTT  >  NC_000913/220748‑220940
                                                                                                 |                                                                                               
cGTCCAGGCCTTATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAATAACCACGCCGACTTTAATGTGGTTTGGATCttt                                                                                               <  2:1813797/100‑1 (MQ=255)
 gTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCtttt                                                                                              >  1:2604178/1‑100 (MQ=255)
   ccAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttttca                                                                                            <  2:668929/100‑3 (MQ=255)
   ccAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttttca                                                                                            >  1:2023309/1‑98 (MQ=255)
    cAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttttcat                                                                                           <  2:801799/100‑4 (MQ=255)
    cAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCa                                                                                           <  2:2680478/100‑1 (MQ=255)
     aGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttttcatc                                                                                          <  1:46588/100‑5 (MQ=255)
      ggCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATcc                                                                                        <  1:412095/100‑1 (MQ=255)
      ggCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATcc                                                                                        >  1:1495498/1‑100 (MQ=255)
        ccATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTg                                                                                      <  1:793961/100‑1 (MQ=255)
           tatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGCCTTTAATGTGGTTTGGATCTTTTCATCCTGAc                                                                                    >  2:1290356/1‑99 (MQ=255)
            atTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCg                                                                                  >  2:1745967/1‑100 (MQ=255)
            atTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCg                                                                                  >  1:193152/1‑100 (MQ=255)
              ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCa                                                                                <  1:266108/100‑1 (MQ=255)
              ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCa                                                                                <  1:2219731/100‑1 (MQ=255)
                gTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGc                                                                              >  1:341737/1‑100 (MQ=255)
                 tCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGcc                                                                             <  2:2405172/100‑1 (MQ=255)
                 tCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGcc                                                                             >  1:2699523/1‑100 (MQ=255)
                  cTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCt                                                                            >  1:1784579/1‑100 (MQ=255)
                    ttCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAc                                                                          >  2:2447974/1‑100 (MQ=255)
                     tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAcc                                                                         >  1:1163212/1‑100 (MQ=255)
                     tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAcc                                                                         >  1:56223/1‑100 (MQ=255)
                      cGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAcca                                                                        <  1:2221175/100‑1 (MQ=255)
                      cGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAcca                                                                        >  1:1700072/1‑100 (MQ=255)
                      cGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGACACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAcca                                                                        <  2:1168171/100‑1 (MQ=255)
                       gCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccag                                                                       <  2:1175558/100‑1 (MQ=255)
                        cAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagt                                                                      <  2:1501928/100‑1 (MQ=255)
                        cAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagn                                                                      <  1:965698/100‑2 (MQ=255)
                            tttCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagtgcca                                                                  <  2:1007892/100‑1 (MQ=255)
                            tttCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagtgcca                                                                  >  2:2184500/1‑100 (MQ=255)
                             ttCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagtgccag                                                                 >  1:1619776/1‑100 (MQ=255)
                                  cgcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATc                                                            <  2:2682615/100‑1 (MQ=255)
                                  cgcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATc                                                            >  1:1044514/1‑100 (MQ=255)
                                  cgcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATc                                                            <  1:468507/100‑1 (MQ=255)
                                   gcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATcc                                                           <  2:939920/100‑1 (MQ=255)
                                   gcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATcc                                                           >  1:1734716/1‑100 (MQ=255)
                                      aCTTCTGCAACCTGCTTTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCg                                                          <  2:2699503/98‑1 (MQ=255)
                                       cTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATc                                                       <  2:1217680/100‑1 (MQ=255)
                                       cTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATc                                                       >  1:747591/1‑100 (MQ=255)
                                       cTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATc                                                       <  1:461473/100‑1 (MQ=255)
                                       cTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATc                                                       <  1:1418137/100‑1 (MQ=255)
                                         tCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAg                                                     >  2:1621805/1‑100 (MQ=255)
                                         tCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAg                                                     <  1:2223446/100‑1 (MQ=255)
                                            gCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAggg                                                   >  1:337160/1‑100 (MQ=255)
                                            gCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGc                                                  >  1:902944/1‑100 (MQ=255)
                                               aCCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTcc                                               >  2:516581/1‑100 (MQ=255)
                                               aCCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTcc                                               >  1:257152/1‑100 (MQ=255)
                                                ccTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCCGCCTCCCAGTGCCAGTGATCCGATCAGCGCTc                                                >  2:1908182/1‑98 (MQ=255)
                                                ccTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTccc                                              >  1:2051860/1‑100 (MQ=255)
                                                 ctgctgTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGTAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCa                                             >  2:2022298/1‑100 (MQ=255)
                                                 ctgctgTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCa                                             <  1:1903943/100‑1 (MQ=255)
                                                  tgctgTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCAc                                            >  1:1573869/1‑100 (MQ=255)
                                                  tgctgTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCAc                                            >  2:1954232/1‑100 (MQ=255)
                                                   gctgTTCGGCACCAACAATCACGCCGACCTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACt                                           <  1:118506/100‑1 (MQ=255)
                                                       ttCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCg                                       <  1:2131865/100‑1 (MQ=255)
                                                        tCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGc                                      <  2:584949/100‑1 (MQ=255)
                                                         cGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCa                                     <  2:2432071/100‑1 (MQ=255)
                                                         cGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCa                                     >  1:1358079/1‑100 (MQ=255)
                                                          ggCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCaa                                    >  2:1667816/1‑100 (MQ=255)
                                                           gCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCaaa                                   >  2:1000759/1‑100 (MQ=255)
                                                              ccAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGt                                <  1:1064204/100‑1 (MQ=255)
                                                              ccAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGt                                >  1:237901/1‑100 (MQ=255)
                                                              ccAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGt                                >  2:1064197/1‑100 (MQ=255)
                                                                  caaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttg                            <  1:653221/100‑1 (MQ=255)
                                                                  caaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttg                            >  2:391904/1‑100 (MQ=255)
                                                                  caaTCACGCCGACTTTAATGGGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCGATTGCCGCAAGGGTtttg                            >  2:2693825/1‑100 (MQ=255)
                                                                    aTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaa                          >  2:2629631/1‑100 (MQ=255)
                                                                       aCGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaattt                       >  1:1700736/1‑100 (MQ=255)
                                                                        cGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaatttg                      <  2:704226/100‑1 (MQ=255)
                                                                        cGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaatttg                      >  2:2162166/1‑100 (MQ=255)
                                                                          ccGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaatttgaa                    <  2:1495487/100‑1 (MQ=255)
                                                                          ccGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGACTACCAGTGCCAGTGATCGGATCAGGGCTCCCACTGCCGCAAAGGTtttgaatttgaa                    >  2:2626097/1‑100 (MQ=255)
                                                                             aCTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGc                 >  1:2238812/1‑100 (MQ=255)
                                                                              cTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGcc                <  2:257149/100‑1 (MQ=255)
                                                                                ttAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCAt              <  2:1044507/100‑1 (MQ=255)
                                                                                ttAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCAt              <  1:1352344/100‑1 (MQ=255)
                                                                                 tAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCaga             >  1:1430352/1‑98 (MQ=255)
                                                                                 tAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATa             >  2:661542/1‑100 (MQ=255)
                                                                                 tAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATa             >  1:1267653/1‑100 (MQ=255)
                                                                                  aaTGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATAc            >  1:2101836/1‑100 (MQ=255)
                                                                                    tgtgGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCt          >  1:1274702/1‑100 (MQ=255)
                                                                                    tgtgGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCt          <  2:1274695/100‑1 (MQ=255)
                                                                                      tgGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTa        >  1:678630/1‑100 (MQ=255)
                                                                                        gTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTAtt      <  2:2558141/100‑1 (MQ=255)
                                                                                        gTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTAtt      >  1:1685601/1‑100 (MQ=255)
                                                                                        gTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTAtt      >  1:2475933/1‑100 (MQ=255)
                                                                                         tttGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTc     >  2:1582952/1‑100 (MQ=255)
                                                                                         tttGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTc     >  1:1333349/1‑100 (MQ=255)
                                                                                         tttGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTc     <  2:1597061/100‑1 (MQ=255)
                                                                                           tGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCct   <  2:193149/100‑1 (MQ=255)
                                                                                            ggATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCctt  >  1:455506/1‑100 (MQ=255)
                                                                                                 |                                                                                               
CGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCCTT  >  NC_000913/220748‑220940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: