Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,018,260 (A)8→7 coding (315/444 nt) fliJ → flagellar protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,018,2530A.97.4% 342.2 / 2.0 77coding (308/444 nt)fliJflagellar protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base . (31/44);  total (32/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAGTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAGATGG  >  NC_000913/2018154‑2018348
                                                                                                   |                                                                                               
agcagTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAACCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCgaga                                                                                                 >  2:638954/1‑100 (MQ=255)
 gcagTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaa                                                                                                >  2:629543/1‑100 (MQ=255)
    gTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaa                                                                                             >  2:865210/1‑100 (MQ=255)
    gTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaa                                                                                             >  1:35637/1‑100 (MQ=255)
     tttATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaa                                                                                            >  2:1896593/1‑100 (MQ=255)
     tttATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaa                                                                                            >  2:775153/1‑100 (MQ=255)
      ttATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaa                                                                                           >  1:1423185/1‑100 (MQ=255)
      ttATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaa                                                                                           >  2:2229144/1‑100 (MQ=255)
      ttATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaa                                                                                           >  1:359545/1‑100 (MQ=255)
       tATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCgagatcggaag                                                                                          >  1:2238901/1‑93 (MQ=255)
       tATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaac                                                                                          <  1:2576513/100‑2 (MQ=255)
       tATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaac                                                                                          >  1:2601499/1‑99 (MQ=255)
       tATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaac                                                                                          >  1:2670509/1‑99 (MQ=255)
        aTCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaaca                                                                                         >  2:313753/1‑98 (MQ=255)
          ccAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaacaac                                                                                       <  2:2463542/100‑5 (MQ=255)
           cAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACg                                                                                     >  1:604549/1‑100 (MQ=255)
           cAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACg                                                                                     >  1:1776920/1‑100 (MQ=255)
              aCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGttt                                                                                  <  1:722674/100‑1 (MQ=255)
               cGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGAGAGAAAAAAACAACGTTTg                                                                                 <  2:876509/100‑1 (MQ=255)
                gCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGc                                                                                <  2:292116/100‑1 (MQ=255)
                 cTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCa                                                                               >  1:180640/1‑100 (MQ=255)
                 cTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCa                                                                               <  1:844875/100‑1 (MQ=255)
                  tGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCa                                                                               <  2:859508/99‑1 (MQ=255)
                   ggAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAgg                                                                             >  1:2157592/1‑100 (MQ=255)
                     aaaaaGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGcc                                                                           <  2:1265446/100‑1 (MQ=255)
                      aaaaGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCt                                                                          >  1:800164/1‑100 (MQ=255)
                       aaaGCCATTACTCATCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAAAAAAAAAAAAAAAAACAACGTTTGCAGGCCTg                                                                         <  2:1881076/100‑1 (MQ=255)
                       aaaGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTg                                                                         <  1:2341007/100‑1 (MQ=255)
                        aaGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTgg                                                                        >  2:58690/1‑100 (MQ=255)
                         aGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGc                                                                       <  1:2511306/100‑1 (MQ=255)
                          gCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCa                                                                      >  1:330946/1‑100 (MQ=255)
                           ccATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAg                                                                     >  2:940826/1‑100 (MQ=255)
                           ccATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAg                                                                     >  1:2215237/1‑100 (MQ=255)
                            cATTGCTCAGCATCGCCAGCAACGTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAg                                                                     <  2:1587168/99‑1 (MQ=255)
                               tACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGacac                                                                 >  2:743285/1‑100 (MQ=255)
                                 cTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTg                                                               >  2:1717063/1‑100 (MQ=255)
                                  tCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGc                                                              <  1:48673/100‑1 (MQ=255)
                                   cAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCa                                                             >  1:1894928/1‑100 (MQ=255)
                                   cAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCa                                                             <  2:2594576/100‑1 (MQ=255)
                                    aGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAg                                                            <  1:876950/100‑1 (MQ=255)
                                    aGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAg                                                            >  2:1830459/1‑100 (MQ=255)
                                       aTCGCCAGCAACTTAATAAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGc                                                              <  2:877239/95‑1 (MQ=255)
                                          gCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACgg                                                      >  1:2514330/1‑100 (MQ=255)
                                          gCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACgg                                                      <  1:644477/100‑1 (MQ=255)
                                           ccAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGc                                                     <  1:1844719/100‑1 (MQ=255)
                                           ccAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGc                                                     <  1:1580863/100‑1 (MQ=255)
                                            cAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCa                                                    <  2:2601480/100‑1 (MQ=255)
                                             aGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCaa                                                   <  1:629548/100‑1 (MQ=255)
                                              gCAACTTAACCAGTGGACGCAGAAAGTTGACACTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAAt                                                  <  1:985998/100‑1 (MQ=255)
                                                 aCTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCa                                               >  1:771254/1‑100 (MQ=255)
                                                 aCTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCa                                               <  1:58691/100‑1 (MQ=255)
                                                  cTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAc                                              <  1:1177325/100‑1 (MQ=255)
                                                  cTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAc                                              <  1:2468635/100‑1 (MQ=255)
                                                  cTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAc                                              <  1:743290/100‑1 (MQ=255)
                                                    tAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcgg                                            >  2:1393680/1‑100 (MQ=255)
                                                     aaTCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcg                                             >  2:985338/1‑98 (MQ=255)
                                                       tCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcggcgg                                         >  1:2746144/1‑100 (MQ=255)
                                                        cAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACggcggc                                        <  2:1331521/100‑1 (MQ=255)
                                                         aGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCa                                       >  1:2295460/1‑100 (MQ=255)
                                                           tGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACt                                     <  1:871360/100‑1 (MQ=255)
                                                             gACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGc                                   <  2:180637/100‑1 (MQ=255)
                                                              aCGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCt                                  <  2:986988/100‑1 (MQ=255)
                                                                gCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTg                                >  2:1254831/1‑100 (MQ=255)
                                                                 cAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               <  2:2204411/100‑1 (MQ=255)
                                                                 cAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               <  1:313756/100‑1 (MQ=255)
                                                                  aGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCa                              >  2:1014088/1‑100 (MQ=255)
                                                                  aGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCa                              <  2:1958724/100‑1 (MQ=255)
                                                                  aGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCa                              <  1:298353/100‑1 (MQ=255)
                                                                    aaaGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGa                            <  1:940832/100‑1 (MQ=255)
                                                                       gTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGaaaa                         <  1:2229162/100‑1 (MQ=255)
                                                                         tGACATTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAAcc                       >  2:2024763/1‑100 (MQ=255)
                                                                             aTTGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCt                   <  2:2140908/100‑1 (MQ=255)
                                                                              ttGCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTc                  >  1:2097859/1‑100 (MQ=255)
                                                                                gCCCTGAACAGTTGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCg                 >  1:877721/1‑100 (MQ=255)
                                                                                gCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGa                >  1:1295501/1‑100 (MQ=255)
                                                                                gCCCTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGa                >  1:1296544/1‑100 (MQ=255)
                                                                                 cccTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGAt               >  1:1099929/1‑100 (MQ=255)
                                                                                  ccTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATc              <  2:1795287/100‑1 (MQ=255)
                                                                                  ccTGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATc              <  1:1830474/100‑1 (MQ=255)
                                                                                  catGAACAGTTGGCGAGAAAAAAACAACGTCTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATc              <  2:702316/98‑1 (MQ=255)
                                                                                    tGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAg            <  1:985345/100‑1 (MQ=255)
                                                                                    tGAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAg            <  2:1045635/100‑1 (MQ=255)
                                                                                     gAACAGTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGa           <  2:981747/100‑1 (MQ=255)
                                                                                          gTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAg      >  1:1813550/1‑100 (MQ=255)
                                                                                          gTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAg      >  2:177937/1‑100 (MQ=255)
                                                                                          gTTGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCGCGATCAGAAAAAg      >  1:1789566/1‑100 (MQ=255)
                                                                                           ttGGCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAga     <  2:1396241/100‑1 (MQ=255)
                                                                                             ggCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatg   >  1:1720634/1‑100 (MQ=255)
                                                                                             ggCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatg   <  1:1896608/100‑1 (MQ=255)
                                                                                             ggCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatg   >  2:1673249/1‑100 (MQ=255)
                                                                                              gCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatgg  <  1:181865/100‑1 (MQ=255)
                                                                                              gCGAGAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatgg  <  2:2274236/100‑1 (MQ=255)
                                                                                                   |                                                                                               
AGCAGTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAGATGG  >  NC_000913/2018154‑2018348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: