Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,639,879 (T)8→7 intergenic (‑159/‑232) uspB ← / → uspA universal stress (ethanol tolerance) protein B/universal stress global response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,639,8720T.96.7% 532.8 / 10.8 122intergenic (‑152/‑239)uspB/uspAuniversal stress (ethanol tolerance) protein B/universal stress global response regulator
Reads supporting (aligned to +/- strand):  ref base T (4/0);  new base . (57/61);  total (61/61)
Fisher's exact test for biased strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

Only 100 of 144 total aligned reads displayed.

CGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTC  >  NC_000913/3639773‑3639967
                                                                                                   |                                                                                               
cGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGgttgt                                                                                                 >  1:584955/1‑100 (MQ=255)
cGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGgttgt                                                                                                 >  1:1848461/1‑100 (MQ=255)
     cGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGttttta                                                                                            >  2:2755096/1‑99 (MQ=255)
      gCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGttttttt                                                                                           <  1:899518/100‑1 (MQ=255)
       cTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttc                                                                                          <  2:2179242/100‑2 (MQ=255)
        tGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcn                                                                                         <  1:686500/100‑3 (MQ=255)
        tGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcc                                                                                         <  2:1524663/100‑3 (MQ=255)
        tGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttc                                                                                          <  2:1925868/99‑2 (MQ=255)
         gCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcca                                                                                        >  1:1116397/1‑97 (MQ=255)
         gCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcca                                                                                        <  2:1471994/100‑4 (MQ=255)
         gCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcca                                                                                        <  1:548683/100‑4 (MQ=255)
         gCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcca                                                                                        >  1:2510384/1‑97 (MQ=255)
         gCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcca                                                                                        <  1:1390733/100‑4 (MQ=255)
         gCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcca                                                                                        >  1:1729192/1‑97 (MQ=255)
         gCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGACTCTATAGATAGTGTAGGAGATCAGGTTGtttttttcca                                                                                        >  1:220828/1‑97 (MQ=255)
          ccAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGtttttttccag                                                                                       <  1:2507705/100‑5 (MQ=255)
              tCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAgg                                                                                  <  2:436291/100‑1 (MQ=255)
               cAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGt                                                                                 >  2:1748640/1‑100 (MQ=255)
                aGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGatcgga                                                                                >  1:1783278/1‑95 (MQ=255)
                aGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGtt                                                                                >  2:2064644/1‑100 (MQ=255)
                aGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCGATATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGAtt                                                                                >  2:2062612/1‑100 (MQ=255)
                  ttAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTaa                                                                              <  1:79323/100‑1 (MQ=255)
                   tAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAAc                                                                             >  2:713259/1‑100 (MQ=255)
                    aaCACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAAcc                                                                            <  2:473570/100‑1 (MQ=255)
                    aaCACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAAcc                                                                            >  2:2089040/1‑100 (MQ=255)
                    aaCACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAAcc                                                                            >  2:722990/1‑100 (MQ=255)
                    aaCACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAAcc                                                                            >  1:1248881/1‑100 (MQ=255)
                    aaCACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAAcc                                                                            >  1:388949/1‑100 (MQ=255)
                      cacCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCAc                                                                          >  1:470007/1‑100 (MQ=255)
                      cacCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTG‑‑TTTTTTCCAGAAGGTTAACCACt                                                                         >  1:2544579/1‑100 (MQ=255)
                         cAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATGAGGTTGTTTTTTTCCAGAAGGTTAACCAGTAt                                                                       >  1:195886/1‑100 (MQ=255)
                             tCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAat                                                                   <  2:1116390/100‑1 (MQ=255)
                              ccTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAata                                                                  >  2:1382694/1‑100 (MQ=255)
                              ccTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAata                                                                  <  1:619208/100‑1 (MQ=255)
                              ccTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAata                                                                  >  1:2651180/1‑100 (MQ=255)
                              ccTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAata                                                                  <  1:2026488/100‑1 (MQ=255)
                              ccTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGATGACCACTATCAat                                                                   >  2:217725/1‑99 (MQ=255)
                               cTGGAGAAACCGCTTTTGTGGTGACCAACATACGATCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAata                                                                  <  2:1905537/99‑1 (MQ=255)
                               cTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAatat                                                                 >  1:397439/1‑100 (MQ=255)
                                tGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAatata                                                                >  1:2285603/1‑100 (MQ=255)
                                tGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAatata                                                                >  1:1477964/1‑100 (MQ=255)
                                    gaAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCa                                                            <  1:1806716/100‑1 (MQ=255)
                                     aaaCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTCACCACTATCAATATATTCAt                                                           <  2:1689900/100‑1 (MQ=255)
                                      aaCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATCGATCGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTc                                                             >  2:2509442/1‑97 (MQ=255)
                                      aaCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCAt                                                           >  2:640184/1‑99 (MQ=255)
                                      aaCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATg                                                          <  1:1404271/100‑1 (MQ=255)
                                      aaCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATg                                                          <  2:658771/100‑1 (MQ=255)
                                      aaCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATg                                                          <  1:1553263/100‑1 (MQ=255)
                                       aCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGt                                                         >  2:1229406/1‑100 (MQ=255)
                                         cGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCg                                                       <  1:2505949/100‑1 (MQ=255)
                                         cGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCg                                                       <  1:321367/100‑1 (MQ=255)
                                             tttGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATTTTCATGTCGaata                                                   <  1:1872239/100‑3 (MQ=255)
                                               tgtgGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAAtt                                                 >  2:631006/1‑100 (MQ=255)
                                                gtggtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAAttt                                                >  2:779074/1‑100 (MQ=255)
                                                 tggtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTTAGAAGTTTAACCACTATCAATATATTCATGTCGAAAATTTg                                               >  2:79773/1‑100 (MQ=255)
                                                 tggtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTg                                               <  1:936130/100‑1 (MQ=255)
                                                 tggtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTg                                               >  1:1654864/1‑100 (MQ=255)
                                                  ggtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGt                                              >  2:690626/1‑100 (MQ=255)
                                                  ggtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGt                                              <  2:2714425/100‑1 (MQ=255)
                                                   gtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGtt                                             <  2:882216/100‑1 (MQ=255)
                                                   gtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGtt                                             >  1:597609/1‑100 (MQ=255)
                                                   gtgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGtt                                             <  1:2192722/100‑1 (MQ=255)
                                                   gtgACCAACATACGACCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGtt                                             <  1:2718734/100‑1 (MQ=255)
                                                    tgACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGttt                                            <  2:388945/100‑1 (MQ=255)
                                                     gACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTa                                           <  2:2708307/100‑1 (MQ=255)
                                                       ccAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATc                                         <  2:2396058/100‑1 (MQ=255)
                                                       ccAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATc                                         >  2:1276801/1‑100 (MQ=255)
                                                        cAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCt                                        <  2:1654851/100‑1 (MQ=255)
                                                         aaCATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGtagatcgg                                       >  2:2437032/1‑93 (MQ=255)
                                                           cATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAAc                                     <  2:470003/100‑1 (MQ=255)
                                                              aCGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAg                                  >  1:2738650/1‑100 (MQ=255)
                                                               cGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGt                                 <  2:2571222/100‑1 (MQ=255)
                                                                 aGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTaa                               <  1:2281818/100‑1 (MQ=255)
                                                                  gCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAg                              <  1:1102950/100‑1 (MQ=255)
                                                                    ggCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGc                             <  2:907963/99‑1 (MQ=255)
                                                                    ggCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCa                            <  1:128368/100‑1 (MQ=255)
                                                                     gCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCaa                           <  2:1623943/100‑1 (MQ=255)
                                                                     gCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCa                            <  2:2383982/99‑1 (MQ=255)
                                                                         tataGATAGTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATAAATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCg                       <  2:2250798/100‑1 (MQ=255)
                                                                          atagatagTGTAGGAGATCAGGTTG‑‑TTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGa                     <  2:2544560/100‑1 (MQ=255)
                                                                            agatagTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACTACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGCGTAAGCAAGGc                        >  2:1737256/1‑96 (MQ=255)
                                                                            agatagTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAAACACTATCAATATATTCATGGCGAAAATTTGTTTATCTAACGAGTAAGCAAGACGGAt                    >  2:592420/1‑100 (MQ=255)
                                                                             gatagTGTAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGAtt                   >  1:188418/1‑100 (MQ=255)
                                                                                 gtgtAGGAGATCAGGTTGTTTTTTTTTTTTTTTTTAACCACTATCAATATATTCATGTTTAAAATTTGTTTATCTAACGAGTAAGCAAGGCTGATTGACg               >  2:2338994/1‑100 (MQ=255)
                                                                                 gtgtAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACg               >  1:1498378/1‑100 (MQ=255)
                                                                                   gtAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGa             >  2:2193692/1‑100 (MQ=255)
                                                                                   gtAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGa             <  1:779079/100‑1 (MQ=255)
                                                                                    tagtagATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGat            <  2:2551768/100‑1 (MQ=255)
                                                                                    tAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGat            >  1:1283435/1‑100 (MQ=255)
                                                                                    tAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGat            <  1:2486513/100‑1 (MQ=255)
                                                                                    tAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGat            <  2:1221308/100‑1 (MQ=255)
                                                                                    tAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGat            >  2:1489411/1‑100 (MQ=255)
                                                                                    tAGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGat            <  2:1833868/100‑1 (MQ=255)
                                                                                     aGGAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTAGTTTAACTAACGAGAAAGCAAGGCGGATTGACGGatc           >  2:1857538/1‑100 (MQ=255)
                                                                                      ggAGATCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGatca          >  2:1470519/1‑100 (MQ=255)
                                                                                         gaTCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATcc       <  1:2509461/100‑1 (MQ=255)
                                                                                          aTCAGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCg      >  2:1316350/1‑100 (MQ=255)
                                                                                             aGGTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt   <  2:1178486/100‑1 (MQ=255)
                                                                                              ggTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCGTcctgtgg  <  2:2310135/100‑6 (MQ=255)
                                                                                              ggTTGTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTc  <  2:1302272/100‑1 (MQ=255)
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CGATACGCTGCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTC  >  NC_000913/3639773‑3639967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: