Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,432,376 (G)5→4 coding (681/966 nt) ytfF ← DMT transporter family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,432,3720G.99.1% 516.4 / ‑2.0 111coding (685/966 nt)ytfFDMT transporter family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (50/60);  total (51/60)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 118 total aligned reads displayed.

GTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCG  >  NC_000913/4432275‑4432467
                                                                                                 |                                                                                               
gTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACggg                                                                                               >  2:861656/1‑100 (MQ=255)
gTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACggg                                                                                               <  2:1338837/100‑1 (MQ=255)
gTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACggg                                                                                               >  1:2230784/1‑100 (MQ=255)
 tCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACgggg                                                                                              <  2:1709255/100‑1 (MQ=255)
   ggTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACggggcc                                                                                            >  1:1806101/1‑98 (MQ=255)
       aTAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAg                                                                                       >  1:2668877/1‑100 (MQ=255)
       aTAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGAGCCAAAg                                                                                       <  2:663333/100‑1 (MQ=255)
       aTAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGAAACCATCAGACTAATAAACACCAGCGGACggggccaa                                                                                          >  1:1773218/1‑94 (MQ=255)
        tAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAgg                                                                                      <  2:1740435/100‑1 (MQ=255)
        tAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAgg                                                                                      <  2:163329/100‑1 (MQ=255)
        tAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAgg                                                                                      >  2:729826/1‑100 (MQ=255)
         aGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAGCCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAggg                                                                                     >  2:1802229/1‑100 (MQ=255)
         aGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAggg                                                                                     <  1:774736/100‑1 (MQ=255)
         aGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAggg                                                                                     <  1:773423/100‑1 (MQ=255)
         aGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAggg                                                                                     <  2:1358012/100‑1 (MQ=255)
          gctggctgGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGt                                                                                    <  2:2371062/100‑1 (MQ=255)
           ctggctggCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTa                                                                                   <  1:2587208/100‑1 (MQ=255)
             ggctggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAgg                                                                                 >  2:2534013/1‑100 (MQ=255)
             ggctggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAgg                                                                                 <  1:729831/100‑1 (MQ=255)
             ggctggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAgg                                                                                 >  1:797009/1‑100 (MQ=255)
             ggctggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAgg                                                                                 >  1:306099/1‑100 (MQ=255)
             ggctggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAgg                                                                                 >  1:2239618/1‑100 (MQ=255)
              gctggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAggg                                                                                <  1:213406/100‑1 (MQ=255)
               ctggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGga                                                                               >  1:794693/1‑100 (MQ=255)
               ctggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGga                                                                               <  1:436878/100‑1 (MQ=255)
                tggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGgag                                                                              >  1:2334910/1‑100 (MQ=255)
                 ggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGgaga                                                                             <  1:2568746/100‑1 (MQ=255)
                  gcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGaa                                                                            <  1:1905922/100‑1 (MQ=255)
                  gcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGaa                                                                            >  2:264724/1‑100 (MQ=255)
                   cGACGTTCCAGCAGAGTGCGCCGACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGgagn                                                                             <  2:2155914/98‑2 (MQ=255)
                   cGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGgaga                                                                             <  1:900482/98‑1 (MQ=255)
                   cGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAg                                                                           <  2:842052/100‑1 (MQ=255)
                    gACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCGATCGCAACCATCAGACTAATAAACACCACGGGAC‑GGGGCCAAAAGGTAGGGAGAAg                                                                           <  1:268589/99‑1 (MQ=255)
                    gACGTGCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGt                                                                          <  2:665709/100‑1 (MQ=255)
                      cGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTcc                                                                        >  1:44923/1‑100 (MQ=255)
                       gTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGTGCCAAAGGGTAGGGAGAAGTCCg                                                                       >  1:1484155/1‑100 (MQ=255)
                        ttCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCgg                                                                      <  1:1168016/100‑1 (MQ=255)
                        ttCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCgg                                                                      <  2:1773204/100‑1 (MQ=255)
                        ttCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCgg                                                                      <  1:2534032/100‑1 (MQ=255)
                         tCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGTCAAAGGGTAGGGAGAAGTCCgg                                                                      <  1:2481814/99‑1 (MQ=255)
                         tCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGc                                                                     >  2:2699771/1‑100 (MQ=255)
                           cagcagAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAGCCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGt                                                                   <  1:1802243/100‑1 (MQ=255)
                            agcagAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGtt                                                                  >  2:1020706/1‑100 (MQ=255)
                            agcagAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGtt                                                                  >  2:708240/1‑100 (MQ=255)
                             gcagAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGttt                                                                 <  2:158885/100‑1 (MQ=255)
                             gcagAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGttt                                                                 >  2:2692314/1‑100 (MQ=255)
                             gcagAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGttt                                                                 >  1:695224/1‑100 (MQ=255)
                              cagAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTg                                                                <  2:190550/100‑1 (MQ=255)
                              cagAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTg                                                                <  2:2411940/100‑1 (MQ=255)
                              cagAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTg                                                                <  1:2692334/100‑1 (MQ=255)
                                gagTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCg                                                              <  2:273503/100‑1 (MQ=255)
                                gagTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCg                                                              <  1:1349764/100‑1 (MQ=255)
                                gagTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCg                                                              <  1:2527860/100‑1 (MQ=255)
                                     cgcCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTc                                                         >  2:1775313/1‑100 (MQ=255)
                                     cgcCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGAGCCAAGGGGTAGGGAGAAGTCCGGCGTTTGCGTATTc                                                         >  2:1858276/1‑100 (MQ=255)
                                         aaCCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGcc                                                     <  2:764862/100‑1 (MQ=255)
                                            ccATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGt                                                  >  1:1164306/1‑100 (MQ=255)
                                             cATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTa                                                 >  2:1214459/1‑100 (MQ=255)
                                             cATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTa                                                 >  2:2006614/1‑100 (MQ=255)
                                             cATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTa                                                 <  1:1654323/100‑1 (MQ=255)
                                             cATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTa                                                 >  1:378280/1‑100 (MQ=255)
                                             cATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTa                                                 >  2:1725339/1‑100 (MQ=255)
                                               tGAGCAAAGCACGGCTATCGCAACCATCAGACTGATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAAc                                               >  1:1908152/1‑100 (MQ=255)
                                                 aGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACaa                                             <  1:1775327/100‑1 (MQ=255)
                                                 aGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACaa                                             <  2:1040770/100‑1 (MQ=255)
                                                  gCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAg                                            >  2:683737/1‑100 (MQ=255)
                                                   cAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCACAGGGGAGGGAGAAGTCCGGCGTGTGCGTATTCAGCCAGTAAc                                               >  2:1171923/1‑96 (MQ=255)
                                                   cAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGc                                           <  1:1687197/100‑1 (MQ=255)
                                                   cAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGc                                           >  1:2424462/1‑100 (MQ=255)
                                                    aaaGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGcg                                          >  1:469567/1‑100 (MQ=255)
                                                       gCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGTGCCAAAGGGTAGGGAGACGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGcgacg                                       <  2:1484144/100‑1 (MQ=255)
                                                        cACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGcgacga                                      <  2:736543/100‑1 (MQ=255)
                                                         tctgCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACgag                                     <  1:1784291/97‑1 (MQ=255)
                                                         aCGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACgag                                     <  2:44922/100‑1 (MQ=255)
                                                            gCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACgagaga                                  >  2:1784277/1‑98 (MQ=255)
                                                               aTCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGcc                               <  2:1633419/100‑1 (MQ=255)
                                                                 cGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGa                             >  2:322955/1‑100 (MQ=255)
                                                                 cGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGa                             >  1:2300202/1‑100 (MQ=255)
                                                                  gCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGAt                            >  1:74303/1‑100 (MQ=255)
                                                                  gCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGAt                            <  2:223974/100‑1 (MQ=255)
                                                                   cAACCATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATa                           >  1:1103731/1‑100 (MQ=255)
                                                                       cATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTg                       <  1:1020713/100‑1 (MQ=255)
                                                                       cATCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTg                       <  1:2699791/100‑1 (MQ=255)
                                                                        aTCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGa                      >  2:2091099/1‑100 (MQ=255)
                                                                        aTCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGa                      >  2:705298/1‑100 (MQ=255)
                                                                         tCAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGaa                     <  2:2181214/100‑1 (MQ=255)
                                                                          cAGACTAATAAACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGaaa                    >  2:2483028/1‑100 (MQ=255)
                                                                                 ataaACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAg             >  2:1292205/1‑100 (MQ=255)
                                                                                 ataaACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAg             <  1:618370/100‑1 (MQ=255)
                                                                                  taaACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCa              >  2:844440/1‑99 (MQ=255)
                                                                                  taaACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAgt            >  2:415612/1‑100 (MQ=255)
                                                                                  taaACACCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAgt            >  2:78432/1‑100 (MQ=255)
                                                                                      cacCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGa        >  2:2230332/1‑100 (MQ=255)
                                                                                      cacCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGa        >  2:1174241/1‑100 (MQ=255)
                                                                                      cacCAGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGa        >  1:1559853/1‑100 (MQ=255)
                                                                                          aGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAg    >  1:1129144/1‑100 (MQ=255)
                                                                                          aGCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAg    <  2:797003/100‑1 (MQ=255)
                                                                                           gCGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAgc   <  1:1068690/100‑1 (MQ=255)
                                                                                            cGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAgtgtagatcgga  >  1:1736262/1‑92 (MQ=255)
                                                                                            cGGAC‑GGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAgcg  <  2:2334892/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCG  >  NC_000913/4432275‑4432467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑