Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,494,883 (A)7→8 coding (261/564 nt) idnK → D‑gluconate kinase, thermosensitive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,494,8761.A96.0% 351.5 / 5.8 103coding (254/564 nt)idnKD‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand):  ref base . (3/1);  new base A (59/38);  total (64/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

ATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑AAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAAT  >  NC_000913/4494778‑4494969
                                                                                                   |                                                                                                
aTCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑a                                                                                                <  2:88905/100‑1 (MQ=255)
aTCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑a                                                                                                <  1:2285677/100‑1 (MQ=255)
  cTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaa                                                                                             >  2:385135/1‑100 (MQ=255)
   tgatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaa                                                                                            <  2:2603602/100‑1 (MQ=255)
   tgatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaa                                                                                            >  2:1295889/1‑100 (MQ=255)
    gatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaa                                                                                           <  1:641183/100‑1 (MQ=255)
     atgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaa                                                                                          <  1:719871/100‑1 (MQ=255)
      tgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaa                                                                                         >  1:2707931/1‑100 (MQ=255)
        aaGATGGAGTTACATGGCTGGAAGGCTTAAATGATGCTGCAGACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaac                                                                                       <  2:2414186/100‑3 (MQ=255)
        aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaac                                                                                       >  1:1329852/1‑98 (MQ=255)
        aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaac                                                                                       <  1:138175/100‑3 (MQ=255)
        aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaac                                                                                       <  1:447570/100‑3 (MQ=255)
        aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaac                                                                                       <  2:1431903/100‑3 (MQ=255)
         aGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaaca                                                                                      >  2:754815/1‑97 (MQ=255)
          gATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaacag                                                                                     <  1:305555/100‑5 (MQ=255)
          gATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaacag                                                                                     <  1:991147/100‑5 (MQ=255)
          gATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaacag                                                                                     >  2:1229038/1‑96 (MQ=255)
              gACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATc                                                                                 >  1:323098/1‑100 (MQ=255)
               aCTTGCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTAGAAAAAGAATGAAACAGAATTTATTGTCTGTTCATCATTAAAAAAAACAGTa                                                                                   <  1:304831/97‑1 (MQ=255)
                cTTCCCTTGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCg                                                                                <  2:1224481/99‑1 (MQ=255)
                 ttCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAAAAGTATCGt                                                                               >  2:174537/1‑99 (MQ=255)
                    ccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGata                                                                           >  2:1027566/1‑100 (MQ=255)
                    ccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGata                                                                           >  2:1832184/1‑100 (MQ=255)
                     ctggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGatat                                                                          >  1:249602/1‑100 (MQ=255)
                     ctggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGata                                                                           >  1:1137541/1‑99 (MQ=255)
                         ctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTa                                                                      >  1:1564782/1‑100 (MQ=255)
                          tggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTAc                                                                     <  1:878372/100‑1 (MQ=255)
                           ggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTCAAAAAAACAGTATCGTGATATTTTACg                                                                    >  2:771166/1‑100 (MQ=255)
                           ggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACg                                                                    <  1:255239/100‑1 (MQ=255)
                           ggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACg                                                                    >  1:1069807/1‑100 (MQ=255)
                            gAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGc                                                                   <  2:847304/100‑1 (MQ=255)
                            gAACGATTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGc                                                                   <  2:1372719/100‑1 (MQ=255)
                               cGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTGAAAAAAACAGTATCGTGATATTTTACGCAAg                                                                >  2:1856560/1‑100 (MQ=255)
                                   tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTTGGCATGGGt                                                             >  2:120993/1‑99 (MQ=255)
                                   tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTa                                                            >  2:2450816/1‑100 (MQ=255)
                                   tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTa                                                            >  1:2141449/1‑100 (MQ=255)
                                    aaaTGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAg                                                           <  2:696906/100‑1 (MQ=255)
                                      atgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGc                                                          >  2:2385799/1‑99 (MQ=255)
                                       tgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGccc                                                        >  1:2282488/1‑100 (MQ=255)
                                       tgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGccc                                                        >  2:2699776/1‑100 (MQ=255)
                                       tgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGccc                                                        >  2:1107800/1‑100 (MQ=255)
                                        gatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGcccc                                                       >  1:1814175/1‑100 (MQ=255)
                                        gatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGcccc                                                       >  1:2281719/1‑100 (MQ=255)
                                        gatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAGT‑‑AGAAAAAAAAGTATCGTGATATTTTACGCAAGGGTAGcccc                                                       >  2:1937917/1‑100 (MQ=255)
                                          tgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCa                                                     <  1:2048275/100‑1 (MQ=255)
                                           gCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCAt                                                    >  1:2039031/1‑100 (MQ=255)
                                            cTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATg                                                   <  1:328796/100‑1 (MQ=255)
                                             ttCATCTAGTGTTTATAAAAAGAATGAAACAGGATTTAGTGTCTGTTCATCATT‑‑AAAAAAAGCAGTAGTGTGATATTTTACGCAAGGGTAGCCCCCAttt                                                  <  2:915456/100‑3 (MQ=255)
                                             ttCATACAGTCTTTATAAAAAGAATGAAACAGGCTTTATTGTCTGTTCATCATTAAAAAAAAACAGTAACGTGATATTTTACGCAAGGGTAGCCCCCATg                                                   >  1:743691/1‑100 (MQ=255)
                                              tCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGtt                                                 <  1:1321824/100‑1 (MQ=255)
                                               cATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGCAGCCCCCATg                                                   >  1:1581152/1‑97 (MQ=255)
                                                aTACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCa                                               >  2:2393179/1‑100 (MQ=255)
                                                 tACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGCTATTTTACGCAAGGGTATCCCCCATGTTCAt                                              <  1:1856575/100‑1 (MQ=255)
                                                 tACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAt                                              >  1:2584217/1‑100 (MQ=255)
                                                 tACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCACCCATGTTCAt                                              >  2:1099800/1‑100 (MQ=255)
                                                   cAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAttt                                            >  2:2648013/1‑100 (MQ=255)
                                                     gTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTcc                                          >  1:2669728/1‑100 (MQ=255)
                                                     gTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTcc                                          >  2:1387337/1‑100 (MQ=255)
                                                         ttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTg                                      <  2:901156/100‑1 (MQ=255)
                                                         ttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTg                                      <  1:1335963/100‑1 (MQ=255)
                                                           ataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGt                                    <  1:1229045/100‑1 (MQ=255)
                                                           ataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGAGTAACCCCCATGTTCATTTCCTCTGGt                                    >  2:672307/1‑100 (MQ=255)
                                                            taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                   >  1:1144764/1‑100 (MQ=255)
                                                            taAAAAAAATGAAACAGGATTCATTTTCTGTTTATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtn                                   <  2:1141617/100‑2 (MQ=255)
                                                             aaaaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTa                                  >  1:2600181/1‑100 (MQ=255)
                                                              aaaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAg                                 >  1:1193479/1‑100 (MQ=255)
                                                               aaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAg                                 >  1:890471/1‑99 (MQ=255)
                                                                ccgatctaaaCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGAt                               <  2:769764/93‑1 (MQ=255)
                                                                   aaTGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGc                            <  1:1832199/100‑1 (MQ=255)
                                                                   aaTGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGc                            >  2:24075/1‑100 (MQ=255)
                                                                     tGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACCGTATCGTTATTTTTTAATCAAAGGGAACCCCCATGGTTATTTTCTTCGGTTAGATTGCGa                          >  2:2334814/1‑100 (MQ=255)
                                                                     tGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGa                          <  2:624077/100‑1 (MQ=255)
                                                                      gAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCCTGCACATTTCCTCTCGTCAGGTgggt                           >  2:896157/1‑97 (MQ=255)
                                                                       aaaCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACt                        >  2:164307/1‑100 (MQ=255)
                                                                        aaCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTa                       <  2:2262030/100‑1 (MQ=255)
                                                                         aCAGGATTTATTGTCTGTTCATCATTAAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTa                       <  1:938975/100‑1 (MQ=255)
                                                                         aCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTAt                      >  2:144711/1‑100 (MQ=255)
                                                                         aCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTAt                      >  2:196640/1‑100 (MQ=255)
                                                                         aCAGGATTTATTGTCTGTTCATCATT‑‑AAAAAAAGCAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATATTCATTTCCTCTGGTTAGATGGCGACTa                       >  2:1666884/1‑99 (MQ=255)
                                                                           aGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGa                    <  2:1581140/100‑1 (MQ=255)
                                                                            ggATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGaa                   <  2:2584198/100‑1 (MQ=255)
                                                                             gATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATGTCCTCTGGTTAGATGGCGACTATGaaa                  >  2:864443/1‑100 (MQ=255)
                                                                             gATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCACCATGTTCATTTCCTCTGGTTAGATGGCGACTATGaaa                  >  2:1114278/1‑100 (MQ=255)
                                                                              aTTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                 <  1:1662631/100‑1 (MQ=255)
                                                                                 tATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTAt              >  2:1303820/1‑100 (MQ=255)
                                                                                  aTTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGAGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTAtt             >  2:2478123/1‑100 (MQ=255)
                                                                                   ttGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtc            >  1:1354711/1‑100 (MQ=255)
                                                                                     gTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtctc          >  2:1983589/1‑100 (MQ=255)
                                                                                     gTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtctc          >  2:2115014/1‑100 (MQ=255)
                                                                                      tCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcg         >  2:236278/1‑100 (MQ=255)
                                                                                       cTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgc        <  2:1564770/100‑1 (MQ=255)
                                                                                        tGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcg       <  1:2128195/100‑1 (MQ=255)
                                                                                         gTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgc      >  1:1634837/1‑100 (MQ=255)
                                                                                         gTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgc      >  1:1900025/1‑100 (MQ=255)
                                                                                          ttCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgcg     <  2:173171/100‑1 (MQ=255)
                                                                                           tcatcaTTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGa    >  2:1362668/1‑100 (MQ=255)
                                                                                            catcatTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGaa   >  1:1983033/1‑100 (MQ=255)
                                                                                            catcatTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGACCCCATGTTCATTTCATATGGTTAGATGGCGACTATGAAACTATTCTCGCGCGaa   >  2:526543/1‑100 (MQ=255)
                                                                                             atcatTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAAt  <  2:1983016/100‑1 (MQ=255)
                                                                                             atcatTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAAt  >  1:322888/1‑100 (MQ=255)
                                                                                                   |                                                                                                
ATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑AAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAAT  >  NC_000913/4494778‑4494969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: