Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,202,110 T→C F611S (TTT→TCT)  yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,202,1100TC98.3% 421.6 / ‑4.6 117F611S (TTT→TCT) yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (62/53);  total (63/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

ACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAATTTTGGCGC  >  NC_000913/2202014‑2202206
                                                                                                |                                                                                                
aCCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTctct                                                                                               <  2:2674917/100‑1 (MQ=255)
 ccTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATtctctc                                                                                              <  2:1160662/100‑1 (MQ=255)
 ccTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATtctctc                                                                                              <  2:391861/100‑1 (MQ=255)
  cTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCa                                                                                             <  1:35178/100‑1 (MQ=255)
  cTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTACGCGGGTGAGGACTATTCTCTCa                                                                                             >  2:1727111/1‑100 (MQ=255)
   tGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAg                                                                                            <  2:1385033/100‑1 (MQ=255)
   tGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAg                                                                                            >  2:1784680/1‑100 (MQ=255)
   tGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAg                                                                                            <  1:1119032/100‑1 (MQ=255)
   tGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAg                                                                                            <  1:586524/100‑1 (MQ=255)
    gTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGt                                                                                           >  1:1464290/1‑100 (MQ=255)
    gTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGt                                                                                           >  2:1376710/1‑100 (MQ=255)
     tatttattGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTa                                                                                          >  2:222776/1‑100 (MQ=255)
     tatttattGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTa                                                                                          <  2:57702/100‑1 (MQ=255)
      atttattGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTAt                                                                                         >  1:151830/1‑100 (MQ=255)
         tattGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCt                                                                                      <  1:1376719/100‑1 (MQ=255)
         tattGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCt                                                                                      >  1:2289994/1‑100 (MQ=255)
         taggggCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCt                                                                                      >  2:1895641/5‑100 (MQ=255)
          attGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTc                                                                                     >  1:2141399/1‑100 (MQ=255)
           ttGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCg                                                                                    <  2:1464279/100‑1 (MQ=255)
            tGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCgg                                                                                   >  2:1143687/1‑100 (MQ=255)
            tGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCgg                                                                                   >  2:832833/1‑100 (MQ=255)
             ggCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCgaa                                                                                  >  2:1678419/1‑98 (MQ=255)
             ggCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGa                                                                                  >  1:2403366/1‑100 (MQ=255)
              gCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGAt                                                                                 >  2:1253967/1‑100 (MQ=255)
              gCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGAt                                                                                 <  1:1572173/100‑1 (MQ=255)
               cacaGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGAtt                                                                                >  1:821512/1‑100 (MQ=255)
               cacaGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGAtt                                                                                >  2:641842/1‑100 (MQ=255)
                acaGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTg                                                                               <  1:832839/100‑1 (MQ=255)
                acaGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTg                                                                               <  1:2527631/100‑1 (MQ=255)
                 caGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTgg                                                                              <  2:2178942/100‑1 (MQ=255)
                  aGTTGCCGCGTGAGATGACTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTCTTCTCTCAGTATCCTCGGATTGGa                                                                             >  2:1251625/1‑100 (MQ=255)
                   gTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGAt                                                                            <  1:13684/100‑1 (MQ=255)
                      gCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATgcg                                                                         <  1:2501168/100‑1 (MQ=255)
                       ccGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATgcgc                                                                        >  2:2426711/1‑100 (MQ=255)
                        cgcgTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCt                                                                       <  2:2621390/100‑1 (MQ=255)
                        cgcgTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCt                                                                       >  1:375278/1‑100 (MQ=255)
                        cgcgTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCt                                                                       >  1:1113213/1‑100 (MQ=255)
                        cgcgTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCt                                                                       >  1:500255/1‑100 (MQ=255)
                        cgcgTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCt                                                                       <  1:121444/100‑1 (MQ=255)
                            tGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCa                                                                   >  1:90414/1‑100 (MQ=255)
                            tGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCa                                                                   <  1:429197/100‑1 (MQ=255)
                              agaTGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAgg                                                                 <  1:677068/100‑1 (MQ=255)
                               gaTGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGt                                                                >  1:2426142/1‑100 (MQ=255)
                               gaTGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGt                                                                >  2:1779201/1‑100 (MQ=255)
                               gaTGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGt                                                                <  1:367006/100‑1 (MQ=255)
                                aTGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTc                                                               >  2:2655401/1‑100 (MQ=255)
                                 tGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTct                                                              <  1:2466238/100‑1 (MQ=255)
                                 tGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTct                                                              <  2:1635576/100‑1 (MQ=255)
                                  ggCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTctg                                                             <  2:2444164/100‑1 (MQ=255)
                                    cTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTctgct                                                           <  1:1411439/100‑1 (MQ=255)
                                       tGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGtt                                                        >  1:2307703/1‑100 (MQ=255)
                                        gACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTg                                                       >  2:497114/1‑100 (MQ=255)
                                        gACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTg                                                       <  1:1652053/100‑1 (MQ=255)
                                         aCCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTgg                                                      <  2:1633035/100‑1 (MQ=255)
                                          ccTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTgg                                                      >  2:1757575/1‑99 (MQ=255)
                                          ccTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGc                                                     <  1:2431886/100‑1 (MQ=255)
                                           cTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCt                                                    >  2:1087744/1‑100 (MQ=255)
                                           cTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCt                                                    >  1:738503/1‑100 (MQ=255)
                                              cTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCtttt                                                 <  1:1890775/100‑1 (MQ=255)
                                                ggCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTc                                               <  1:715960/100‑1 (MQ=255)
                                                ggCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTc                                               <  1:1607436/100‑1 (MQ=255)
                                                 gCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTca                                              <  1:10431/100‑1 (MQ=255)
                                                 gCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTca                                              <  2:2650144/100‑1 (MQ=255)
                                                    gCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACAt                                           >  1:594147/1‑100 (MQ=255)
                                                        aTCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTc                                       >  2:1251400/1‑100 (MQ=255)
                                                          cAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCa                                     >  1:2691833/1‑100 (MQ=255)
                                                           aaTGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCaa                                    >  1:2391632/1‑100 (MQ=255)
                                                            aTGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCaaa                                   <  2:289895/100‑1 (MQ=255)
                                                            aTGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCaaa                                   >  2:111619/1‑100 (MQ=255)
                                                               agaAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGa                                >  2:1909653/1‑100 (MQ=255)
                                                               agaAAAAGAATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGa                                >  2:1515112/1‑100 (MQ=255)
                                                                gaAAAAGCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGaa                               <  1:398433/100‑1 (MQ=255)
                                                                      gCATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACAttt                         >  1:732830/1‑100 (MQ=255)
                                                                       cATCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTc                        <  2:257820/100‑1 (MQ=255)
                                                                        aTCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTcc                       >  1:1915662/1‑100 (MQ=255)
                                                                         tCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCg                      <  2:732825/100‑1 (MQ=255)
                                                                         tCTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCg                      <  1:2431993/100‑1 (MQ=255)
                                                                          cTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGt                     >  2:2333735/1‑100 (MQ=255)
                                                                          cTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGt                     >  1:154043/1‑100 (MQ=255)
                                                                          cTGTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGt                     >  2:1032421/1‑100 (MQ=255)
                                                                            gTTCGCGGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTa                   >  1:2415463/1‑100 (MQ=255)
                                                                               cgcgGGTGAGGACTATTTTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAAtt                <  1:823063/100‑1 (MQ=255)
                                                                               cgcgGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTCCCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACCTTTCCGTTAAtt                >  2:1924538/1‑100 (MQ=255)
                                                                               cgcgGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAAtt                <  1:641847/100‑1 (MQ=255)
                                                                               cgcgGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAAtt                <  2:2316319/100‑1 (MQ=255)
                                                                               cgcgGGTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAAtt                <  2:1512596/100‑1 (MQ=255)
                                                                                    gTGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTaaa           <  1:948644/100‑1 (MQ=255)
                                                                                     tGAGGACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAAt          <  1:2655421/100‑1 (MQ=255)
                                                                                        ggACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAAtttt       >  1:1484376/1‑100 (MQ=255)
                                                                                        ggACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAAtttt       >  2:714199/1‑100 (MQ=255)
                                                                                        ggACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAAtttt       >  2:1222483/1‑100 (MQ=255)
                                                                                         gACTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAATTTTg      >  2:2022285/1‑100 (MQ=255)
                                                                                          aCTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAATTTTgg     <  2:594142/100‑1 (MQ=255)
                                                                                          aCTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAATTTTgg     <  1:108936/100‑1 (MQ=255)
                                                                                          aCTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAATTTTgg     <  1:1784694/100‑1 (MQ=255)
                                                                                          aCTATTCTCTCAGTATCCTCGGATCGGATGCGCTACCAGGTCTGCCGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAATTTTgg     <  1:1895656/100‑1 (MQ=255)
                                                                                           cTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAATTTTgg     <  2:2307685/99‑1 (MQ=255)
                                                                                           cTATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAATTTTGgc    <  1:1442521/100‑1 (MQ=255)
                                                                                            tATTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAATTTTGgcg   >  2:2003864/1‑100 (MQ=255)
                                                                                             aTTCTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAATTTTGgcgc  >  1:550645/1‑100 (MQ=255)
                                                                                                |                                                                                                
ACCTGTATTTATTGGCACAGTTGCCGCGTGAGATGGCTTTGACCTTCTGGCTGCGTATCAATGAGAAAAAGCATCTGTTCGCGGGTGAGGACTATTTTCTCAGTATCCTCGGATTGGATGCGCTACCAGGTCTGCTGTTGGCTTTTTCACATCGTCCAAAAGAAACATTTCCGTTAATTTTAAATTTTGGCGC  >  NC_000913/2202014‑2202206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: