Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,516,689	T→C	T382A (ACG→GCG)	yfeA ←	putative diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,516,689	0	T	C	100.0%	301.0 / NA	83	T382A (ACG→GCG)	yfeA	putative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/43);  total (40/43)

GTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGTACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCT  >  NC_000913/2516593‑2516784                                                                                                 |                                                                                                gTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACg                                                                                              >  1:834560/1‑100 (MQ=255)  tCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACgg                                                                                             <  2:1055415/100‑1 (MQ=255)  tCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACgg                                                                                             >  1:663890/1‑100 (MQ=255)   cGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGc                                                                                            <  1:451419/100‑1 (MQ=255)   cGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGc                                                                                            >  1:18741/1‑100 (MQ=255)         tCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTg                                                                                      <  2:2171882/100‑1 (MQ=255)         nccGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTg                                                                                      >  2:1432810/2‑100 (MQ=255)          ccGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGa                                                                                     >  1:2336962/1‑100 (MQ=255)             tttCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCg                                                                                  <  1:1254758/100‑1 (MQ=255)             tttCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCg                                                                                  <  2:1630809/100‑1 (MQ=255)             tttCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCg                                                                                  <  1:860351/100‑1 (MQ=255)              ttCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCgg                                                                                 <  1:559810/100‑1 (MQ=255)                     ccGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGt                                                                          <  2:2336944/100‑1 (MQ=255)                     ccGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGt                                                                          <  1:2383608/100‑1 (MQ=255)                     ccGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGt                                                                          <  1:1492374/100‑1 (MQ=255)                     ccGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGt                                                                          >  2:2000078/1‑100 (MQ=255)                      cGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAGCGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTg                                                                         <  1:2114652/100‑1 (MQ=255)                      cGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTg                                                                         <  1:1046363/100‑1 (MQ=255)                      cGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTg                                                                         >  1:2154730/1‑100 (MQ=255)                       gCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGa                                                                        >  1:2152288/1‑100 (MQ=255)                          cAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcg                                                                     <  1:2448639/100‑1 (MQ=255)                          cAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcg                                                                     >  2:2342824/1‑100 (MQ=255)                          cAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCAGAtcggaagagca                                                                     >  1:837452/1‑90 (MQ=255)                            gCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcgcg                                                                   >  2:29243/1‑100 (MQ=255)                              aCTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGca                                                                 >  1:199019/1‑100 (MQ=255)                               cTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcat                                                                >  2:1659211/1‑100 (MQ=255)                                 aaCAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTTCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcatca                                                              >  2:1538557/1‑100 (MQ=255)                                 aaCAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcatca                                                              >  1:1938544/1‑100 (MQ=255)                                  aCAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcatcat                                                             <  1:84898/100‑1 (MQ=255)                                   cagcagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCAtt                                                            >  1:714905/1‑100 (MQ=255)                                   cagcagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCAtt                                                            <  2:2431956/100‑1 (MQ=255)                                    agcagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTa                                                           >  2:2615848/1‑100 (MQ=255)                                    agcagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTa                                                           <  2:2173861/100‑1 (MQ=255)                                      cagTTCACTACCCGTCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAg                                                         <  2:1879304/100‑1 (MQ=255)                                      cagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAg                                                         <  1:1018982/100‑1 (MQ=255)                                      cagTTCACTACCCCGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCATCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCAtt                                                            <  1:613082/97‑1 (MQ=255)                                       agTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGc                                                        <  2:2154712/100‑1 (MQ=255)                                       agTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGc                                                        >  1:2530574/1‑100 (MQ=255)                                        gTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGcc                                                       >  1:488325/1‑100 (MQ=255)                                        gTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGcc                                                       <  1:867102/100‑1 (MQ=255)                                        gTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGcc                                                       <  2:607757/100‑1 (MQ=255)                                         ttCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCg                                                      <  1:32457/100‑1 (MQ=255)                                          tCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGt                                                     <  1:130760/100‑1 (MQ=255)                                          tCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGt                                                     >  1:1346549/1‑100 (MQ=255)                                          tCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGt                                                     >  1:1346362/1‑100 (MQ=255)                                           cACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTa                                                    >  2:1440383/1‑100 (MQ=255)                                           cACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGACCGGATACAGTGAACGCGCAACATTAAGCCGTa                                                    >  2:2341355/1‑100 (MQ=255)                                             cTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAAt                                                  >  2:1570496/1‑100 (MQ=255)                                               aCCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATga                                                >  2:315932/1‑100 (MQ=255)                                               aCCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATga                                                <  2:714900/100‑1 (MQ=255)                                               aCCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATga                                                >  2:728738/1‑100 (MQ=255)                                                cccGGCAATTGATACAACTTTTTTTTTTTTTTTTTCAACGGCAGCAGCGCACGGCAAATTTAGTGGATACAGTGAACGCGCATCATTAAGCCGTAATgac                                               >  2:2262756/1‑100 (MQ=255)                                                cccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgac                                               <  1:2322841/100‑1 (MQ=255)                                                cccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgac                                               >  2:2517433/1‑100 (MQ=255)                                                cccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgac                                               >  2:1537337/1‑100 (MQ=255)                                                cccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgac                                               >  1:283759/1‑100 (MQ=255)                                                 ccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacg                                              <  1:732905/100‑1 (MQ=255)                                                  cGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacga                                             >  1:2669409/1‑100 (MQ=255)                                                  cGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacga                                             >  2:483704/1‑100 (MQ=255)                                                    gCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACt                                           <  2:139715/100‑1 (MQ=255)                                                       aTTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCAt                                        <  2:824753/100‑1 (MQ=255)                                                        ttGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATa                                       <  1:1847192/100‑1 (MQ=255)                                                           aTACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAAc                                    <  1:1570508/100‑1 (MQ=255)                                                            tACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACt                                   >  2:1316457/1‑100 (MQ=255)                                                            tACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACt                                   >  2:1027177/1‑100 (MQ=255)                                                              cAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCa                                 <  2:2320014/100‑1 (MQ=255)                                                              cAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCa                                 >  1:2008827/1‑100 (MQ=255)                                                                aCTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCaa                                >  1:1107541/1‑99 (MQ=255)                                                                aCTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAg                               <  2:2669389/100‑1 (MQ=255)                                                                  ttttCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGAt                             <  1:365750/100‑1 (MQ=255)                                                                          ttgcggCATCAACGGCAGCGGCGCACGGCAAATTGAGCGGATACAGTGAAGGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATg                     <  2:2415036/95‑1 (MQ=255)                                                                          ttCCTGCATCAACGGCAGCAGCGCAAGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATg                     <  2:2160901/100‑1 (MQ=255)                                                                            ccTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATgcg                   <  1:315935/100‑1 (MQ=255)                                                                             cTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATgcgc                  >  1:1441058/1‑100 (MQ=255)                                                                              tGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgca                 >  2:2027178/1‑100 (MQ=255)                                                                                cATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcagg               <  2:1023461/100‑1 (MQ=255)                                                                                 aTCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcaggc              <  2:1353909/100‑1 (MQ=255)                                                                                 aTCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcaggc              >  2:2463776/1‑100 (MQ=255)                                                                                 aTCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcaggc              <  2:1880504/100‑1 (MQ=255)                                                                                         cagcagCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCaaaaa      >  2:1560107/1‑100 (MQ=255)                                                                                          agcagcGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAAc     <  1:2342842/100‑1 (MQ=255)                                                                                          agcagcGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAAc     <  1:1537349/100‑1 (MQ=255)                                                                                             agcGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACTAGTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAActct  <  1:1360463/100‑1 (MQ=255)                                                                                                 |                                                                                                GTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGTACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCT  >  NC_000913/2516593‑2516784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑