Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,790,586	T→C	L202P (CTG→CCG)	lhgO →	L‑2‑hydroxyglutarate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,790,586	0	T	C	100.0%	363.5 / NA	98	L202P (CTG→CCG)	lhgO	L‑2‑hydroxyglutarate oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (53/45);  total (53/45)

AGTCAGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCATA  >  NC_000913/2790491‑2790681                                                                                                |                                                                                                aGTCAGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGAGAATATGAAGCATCAACGCTGATTAGCTTTTCCGGGCCGagg                                                                                             <  1:1862449/100‑3 (MQ=255)    cAGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCt                                                                                          >  1:2453777/1‑100 (MQ=255)    cAGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCt                                                                                          <  1:500553/100‑1 (MQ=255)     aGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTATCTGTTCCGGGCCGATGGCTg                                                                                         <  2:1688016/100‑1 (MQ=255)     aGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTg                                                                                         >  2:2129937/1‑100 (MQ=255)        gggCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGAcc                                                                                      >  2:685497/1‑100 (MQ=255)           cTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGc                                                                                   <  1:5023/100‑1 (MQ=255)           cTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGc                                                                                   >  1:1522666/1‑100 (MQ=255)            ttAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCt                                                                                  <  1:493673/100‑1 (MQ=255)              aaTGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTgg                                                                                >  1:1478715/1‑100 (MQ=255)               aTGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGt                                                                               <  1:466183/100‑1 (MQ=255)               aTGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGt                                                                               <  1:527700/100‑1 (MQ=255)               aTGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGt                                                                               >  1:30341/1‑100 (MQ=255)                 gAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGa                                                                             <  2:2453758/100‑1 (MQ=255)                 gAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGa                                                                             >  2:2474763/1‑100 (MQ=255)                  aGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGaa                                                                            >  1:1929538/1‑100 (MQ=255)                   gCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGaaa                                                                           <  2:1184441/100‑1 (MQ=255)                     aTAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAAt                                                                         >  2:395891/1‑100 (MQ=255)                      tAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATg                                                                        >  2:2502040/1‑100 (MQ=255)                      tAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATg                                                                        >  1:363040/1‑100 (MQ=255)                       aaaaaCGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGc                                                                       >  2:1236878/1‑100 (MQ=255)                        aaaaCGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCt                                                                      >  1:1819560/1‑100 (MQ=255)                        aaaaCGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCt                                                                      >  2:296660/1‑100 (MQ=255)                          aaCGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCg                                                                    <  1:2058989/100‑1 (MQ=255)                          aaCGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCg                                                                    <  2:431852/100‑1 (MQ=255)                              gCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACt                                                                >  1:450910/1‑100 (MQ=255)                               cGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTc                                                               >  1:2234829/1‑100 (MQ=255)                               cGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTc                                                               >  2:702554/1‑100 (MQ=255)                                gtggtgATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCg                                                              >  2:188189/1‑100 (MQ=255)                                   gtgATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAAc                                                           >  1:2512891/1‑100 (MQ=255)                                    tgATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAAcc                                                          >  2:154405/1‑100 (MQ=255)                                     gATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCg                                                         >  2:2003829/1‑100 (MQ=255)                                       tACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCggg                                                       <  1:183776/100‑1 (MQ=255)                                       tACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTAGGACTCGAACCggg                                                       >  2:1881842/1‑100 (MQ=255)                                        aCGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGc                                                      <  2:1329806/100‑1 (MQ=255)                                         cGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCt                                                     >  1:1137875/1‑100 (MQ=255)                                         cGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCt                                                     >  1:318845/1‑100 (MQ=255)                                         cGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCt                                                     <  1:154408/100‑1 (MQ=255)                                          gTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCtt                                                    <  1:1980027/100‑1 (MQ=255)                                           tACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCttt                                                   >  2:1967461/1‑100 (MQ=255)                                             cccGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTat                                                 >  2:1081697/1‑100 (MQ=255)                                             cccGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTat                                                 >  1:679233/1‑100 (MQ=255)                                              ccGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTatc                                                >  2:898960/1‑100 (MQ=255)                                               cGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTatca                                               >  1:1358556/1‑100 (MQ=255)                                               cGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTatca                                               >  1:1358577/1‑100 (MQ=255)                                                ntCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTatcat                                              >  2:1213689/2‑100 (MQ=255)                                                gTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTatcat                                              <  1:2461036/100‑1 (MQ=255)                                                 tCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTatcatc                                             <  2:192285/100‑1 (MQ=255)                                                  cAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCt                                            <  1:2062523/100‑1 (MQ=255)                                                       cggcgAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCg                                       <  1:2129954/100‑1 (MQ=255)                                                       cggcgAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCg                                       >  2:2734608/1‑100 (MQ=255)                                                          cgAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTc                                    <  1:1839113/100‑1 (MQ=255)                                                             atatGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGt                                 >  1:1601518/1‑100 (MQ=255)                                                              tatGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGt                                 >  2:1930732/1‑99 (MQ=255)                                                              tatGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTg                                <  1:1473193/100‑1 (MQ=255)                                                              tatGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTg                                <  1:1256994/100‑1 (MQ=255)                                                              tatGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTg                                <  1:2618906/100‑1 (MQ=255)                                                              tatGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTg                                <  1:1236885/100‑1 (MQ=255)                                                               atGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTgg                               <  2:2135105/100‑1 (MQ=255)                                                               atGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTgg                               <  2:2134654/100‑1 (MQ=255)                                                                tGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGc                              >  1:2507700/1‑100 (MQ=255)                                                                tGAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGc                              >  2:597538/1‑100 (MQ=255)                                                                 gAAGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCg                             >  1:1318293/1‑100 (MQ=255)                                                                  aaGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGa                            <  1:1164583/100‑1 (MQ=255)                                                                  aaGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGa                            <  1:2032412/100‑1 (MQ=255)                                                                  aaGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTG‑AAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAg                           <  1:2014296/100‑1 (MQ=255)                                                                   aGCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAg                           >  1:1535077/1‑100 (MQ=255)                                                                    gCATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGt                          >  1:2247499/1‑100 (MQ=255)                                                                     cATCAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTa                         >  1:1007315/1‑100 (MQ=255)                                                                       tCAACTCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTAtt                       >  1:2166650/1‑100 (MQ=255)                                                                       tCAACGCTGATTAGCTGTTCCGGGACGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTAtt                       <  2:553738/100‑1 (MQ=255)                                                                        cAACGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTAttt                      >  2:530796/1‑100 (MQ=255)                                                                         aaCGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTc                     <  1:915142/100‑1 (MQ=255)                                                                         aaCGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTc                     <  1:2585242/100‑1 (MQ=255)                                                                           cGCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCg                   <  1:296663/100‑1 (MQ=255)                                                                            gCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGc                  >  2:2636461/1‑100 (MQ=255)                                                                            gCTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCGGGTGAAAGTGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGCGGCGAGTATTTcc                    <  2:1711699/98‑1 (MQ=255)                                                                             cTGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGcc                 >  1:496985/1‑100 (MQ=255)                                                                              tGATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCt                >  1:1478566/1‑100 (MQ=255)                                                                               gATTAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTACCCGTTCCGTGGCGAGTATTTCCGCCtt               >  1:221360/1‑100 (MQ=255)                                                                                 ttAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTgc             >  1:1836371/1‑100 (MQ=255)                                                                                  tAGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTgcg            >  1:634662/1‑100 (MQ=255)                                                                                   aGCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCGCGTTCCGTGGCGAGTATTTCCGCCTTgcg            >  2:2300355/1‑99 (MQ=255)                                                                                    gCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGcc          >  1:1346693/1‑100 (MQ=255)                                                                                    gCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGcc          <  1:611396/100‑1 (MQ=255)                                                                                    gCTGTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGcc          <  2:305785/100‑1 (MQ=255)                                                                                       gTTCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGa       >  2:2745845/1‑100 (MQ=255)                                                                                        ttCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAg      <  2:2449645/100‑1 (MQ=255)                                                                                        ttCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAg      <  1:2211408/100‑1 (MQ=255)                                                                                        ttCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAg      <  2:363036/100‑1 (MQ=255)                                                                                        ttCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAg      <  1:898406/100‑1 (MQ=255)                                                                                        ttCCGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCACCGGAg      <  1:296115/100‑1 (MQ=255)                                                                                          ccGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCa    <  1:289552/100‑1 (MQ=255)                                                                                          ccGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCa    <  1:2321445/100‑1 (MQ=255)                                                                                          ccGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCa    <  1:290012/100‑1 (MQ=255)                                                                                           cGGGCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCAt   >  1:1224426/1‑100 (MQ=255)                                                                                            gggCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCTCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCATa  <  2:1265739/100‑1 (MQ=255)                                                                                            gggCCGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCATa  >  1:1204879/1‑100 (MQ=255)                                                                                                |                                                                                                AGTCAGCGGGCTTAATGAGCATAAAAACGGCGTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCATA  >  NC_000913/2790491‑2790681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑