Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,298,809 A→G V13A (GTG→GCG)  yraR ← putative nucleoside‑diphosphate‑sugar epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,298,8090AG96.4% 289.5 / ‑0.3 84V13A (GTG→GCG) yraRputative nucleoside‑diphosphate‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (42/39);  total (44/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAAT  >  NC_000913/3298712‑3298905
                                                                                                 |                                                                                                
aaaCACCTCGGGCATATCGCCCAACGGACGTCGCGTCTGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACTGCccacc                                                                                                >  2:1484944/1‑100 (MQ=255)
 aaCACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCccgcca                                                                                               <  1:2235877/100‑1 (MQ=255)
 aaCACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCccgcca                                                                                               >  1:382171/1‑100 (MQ=255)
    acCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGcc                                                                                            >  1:780683/1‑100 (MQ=255)
     ccccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGccc                                                                                           >  1:1107751/1‑100 (MQ=255)
     ccccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGccc                                                                                           >  1:2380094/1‑100 (MQ=255)
      cccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGcccc                                                                                          >  1:2071809/1‑100 (MQ=255)
       ccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCg                                                                                         <  2:2271998/100‑1 (MQ=255)
        cGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGt                                                                                        >  1:2699410/1‑100 (MQ=255)
        cGGGCATATCGCCCAACGGACGTCGCGGCGGCGCAGAAATGGCGTTAACTTGCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGt                                                                                        <  2:2602073/100‑1 (MQ=255)
           gCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTgc                                                                                     <  1:1489619/100‑1 (MQ=255)
            cATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTgcg                                                                                    >  2:132682/1‑100 (MQ=255)
             atatCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTgcgc                                                                                   >  1:497591/1‑100 (MQ=255)
              tatCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGcc                                                                                  <  2:649363/100‑1 (MQ=255)
                tCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCTCGCCAGCCCCGTTGCGCCTg                                                                                <  2:1686988/100‑1 (MQ=255)
                tCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTg                                                                                <  2:638521/100‑1 (MQ=255)
                tCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTg                                                                                <  2:762953/100‑1 (MQ=255)
                 cGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGt                                                                               <  1:268628/100‑1 (MQ=255)
                   cccAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTaa                                                                             >  2:1390324/1‑100 (MQ=255)
                   cccAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTaa                                                                             <  2:9110/100‑1 (MQ=255)
                    ccAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGccactggn                                                                            <  2:2053506/100‑7 (MQ=255)
                      aaCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCa                                                                          >  1:597218/1‑100 (MQ=255)
                      aaCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCa                                                                          <  1:1693063/100‑1 (MQ=255)
                         ggACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTa                                                                       <  1:273123/100‑1 (MQ=255)
                          gACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTAc                                                                      >  1:519264/1‑100 (MQ=255)
                            cgtcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTAccg                                                                    >  1:1644752/1‑99 (MQ=255)
                             gtcgcgtcgGCGCAGCAATGGCGTTAACTTGCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTg                                                                   <  2:363949/100‑1 (MQ=255)
                                gcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACt                                                                <  1:1943614/100‑1 (MQ=255)
                                 cgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTc                                                               <  2:702958/100‑1 (MQ=255)
                                  gtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACt                                                                <  1:1930951/98‑1 (MQ=255)
                                     ggCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCt                                                           <  2:1820591/100‑1 (MQ=255)
                                     ggCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCt                                                           <  2:663743/100‑1 (MQ=255)
                                      gcgcAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCtt                                                          <  2:539601/100‑1 (MQ=255)
                                       ngcagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTc                                                         >  1:1330419/2‑100 (MQ=255)
                                        gcagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCg                                                        >  1:598817/1‑100 (MQ=255)
                                         cagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGc                                                       <  2:2677003/100‑1 (MQ=255)
                                         cagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGc                                                       >  1:73213/1‑100 (MQ=255)
                                         cagcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGc                                                       >  2:329817/1‑100 (MQ=255)
                                          agcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCt                                                      <  2:1339853/100‑1 (MQ=255)
                                          agcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCt                                                      <  2:55456/100‑1 (MQ=255)
                                          agcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCt                                                      >  1:108366/1‑100 (MQ=255)
                                            caATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTcc                                                    >  2:1589/1‑100 (MQ=255)
                                              aTGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCtt                                                  <  2:947396/100‑1 (MQ=255)
                                               tGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCttt                                                 <  2:1254325/100‑1 (MQ=255)
                                               tGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCttt                                                 <  1:1089477/100‑1 (MQ=255)
                                               tGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCttt                                                 <  2:2522548/100‑1 (MQ=255)
                                                ggCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTa                                                <  2:103732/100‑1 (MQ=255)
                                                ggCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTa                                                >  1:1469738/1‑100 (MQ=255)
                                                ggCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTa                                                >  2:325686/1‑100 (MQ=255)
                                                ggCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTa                                                <  1:469493/100‑1 (MQ=255)
                                                 gCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTAc                                               <  2:1848706/100‑1 (MQ=255)
                                                  cGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACa                                              <  2:2296462/100‑1 (MQ=255)
                                                  cGTTAACCTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACa                                              >  1:1450354/1‑100 (MQ=255)
                                                   gTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAg                                             <  1:1151558/100‑1 (MQ=255)
                                                    ttAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGa                                            >  1:643510/1‑100 (MQ=255)
                                                     tAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGaa                                           >  2:2358547/1‑100 (MQ=255)
                                                      aaCTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTACTCAGTACCTGCCTCAGCTTCGCACCTTTACACaa                                           >  2:2713441/1‑99 (MQ=255)
                                                       aCTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAAtt                                         >  1:2088447/1‑100 (MQ=255)
                                                       aCTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAAtt                                         >  2:14505/1‑100 (MQ=255)
                                                        cTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTg                                        >  1:1967416/1‑100 (MQ=255)
                                                        cTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTg                                        >  1:539880/1‑100 (MQ=255)
                                                            cGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTg                                    <  2:1785585/100‑1 (MQ=255)
                                                             ggTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCGCCTTTACAGAATTGTCTGc                                   >  2:1371763/1‑100 (MQ=255)
                                                                 cGTTAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTg                               <  2:376312/100‑1 (MQ=255)
                                                                 cGTTAATCAACATCCGCAGCAGGTGACCGCCCGACAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATGGTCTGCCTTg                               >  2:1817712/1‑100 (MQ=255)
                                                                 cGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTTATCTTCGCTCCTTTACAGAATTGTCTGCCTTg                               >  2:1942737/1‑100 (MQ=255)
                                                                    tAATCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgcgc                            <  2:382167/100‑1 (MQ=255)
                                                                     aaTCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCt                           >  2:2186284/1‑100 (MQ=255)
                                                                     aaTCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCt                           <  2:757805/100‑1 (MQ=255)
                                                                      aTCAACATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTc                          >  1:1185508/1‑100 (MQ=255)
                                                                         aaCATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCAc                       >  2:1604759/1‑100 (MQ=255)
                                                                         aaCATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCAc                       >  1:1758342/1‑100 (MQ=255)
                                                                           cATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACgg                     >  2:2640236/1‑100 (MQ=255)
                                                                           cATCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACgg                     <  2:404798/100‑1 (MQ=255)
                                                                            aTCCGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCAGCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGc                    <  2:579160/100‑1 (MQ=255)
                                                                               cacagcagGTGACCGCCCGCAAGCCCCGTTGCGCCTGTAATCAGTAACTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGc                    >  2:425946/3‑97 (MQ=255)
                                                                               cGCAGCAGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCa                 <  2:1264704/100‑1 (MQ=255)
                                                                                gcagcaGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCAt                >  1:643823/1‑100 (MQ=255)
                                                                                gcagcaGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCAt                <  1:2625848/100‑1 (MQ=255)
                                                                                gcagcaGGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCAt                >  1:632211/1‑100 (MQ=255)
                                                                                    cagGTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCa             <  1:1484955/99‑1 (MQ=255)
                                                                                      ggTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCa          >  1:2094565/1‑100 (MQ=255)
                                                                                      ggTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCa          >  1:2048744/1‑100 (MQ=255)
                                                                                      ggTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCa          >  1:2701665/1‑100 (MQ=255)
                                                                                       gTGACCGCCCGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAg         >  2:384127/1‑100 (MQ=255)
                                                                                         ccaccaccaccaGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCACGc       >  2:549906/7‑100 (MQ=255)
                                                                                           ccgcccgcCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCCGGCtt     >  1:476845/1‑100 (MQ=255)
                                                                                           ccgcccgcCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCtt     >  2:232752/1‑100 (MQ=255)
                                                                                           ccgcccgcCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCtt     <  2:1893745/100‑1 (MQ=255)
                                                                                           ccgcccgcCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCtt     <  1:1028286/100‑1 (MQ=255)
                                                                                           ccGCCCACCAGCCCCGTTGCGCCTGTAATCAGAACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCtt     >  1:2617585/1‑100 (MQ=255)
                                                                                            cgcccgccAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTa    <  2:992638/100‑1 (MQ=255)
                                                                                              cccGCCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAAt  >  2:959409/1‑100 (MQ=255)
                                                                                                 |                                                                                                
AAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAAT  >  NC_000913/3298712‑3298905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑