BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,267,564	T→C	Y151H (TAT→CAT)	tyrB →	tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,267,564	0	T	C	100.0%	431.8 / NA	117	Y151H (TAT→CAT)	tyrB	tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (63/54); total (63/54)

Only 100 of 117 total aligned reads displayed.

CTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTG  >  NC_000913/4267467‑4267658

cTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCAt                                                                                              >  1:258276/1‑100 (MQ=255)

 ttCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATg                                                                                             >  1:2005890/1‑100 (MQ=255)

 ttCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATg                                                                                             >  1:2012233/1‑100 (MQ=255)

  tCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGa                                                                                            >  1:348592/1‑100 (MQ=255)

   cccGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTTCCCCTGGCATggc                                                                                           <  1:1874084/100‑3 (MQ=255)

       gAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAg                                                                                       >  2:2656327/1‑100 (MQ=255)

        aaTCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGc                                                                                      <  2:1849839/100‑1 (MQ=255)

         aTCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCg                                                                                     <  1:1766690/100‑1 (MQ=255)

           cAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGAc                                                                                   >  2:303809/1‑100 (MQ=255)

             ggCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTa                                                                                 >  2:85458/1‑100 (MQ=255)

             ggCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTa                                                                                 >  1:2048819/1‑100 (MQ=255)

             ggCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTa                                                                                 <  1:700691/100‑1 (MQ=255)

               cGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAAc                                                                               <  1:1310334/100‑1 (MQ=255)

                   tGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCg                                                                           <  2:42192/100‑1 (MQ=255)

                    gggTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGt                                                                          >  1:2748683/1‑100 (MQ=255)

                    gggTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGt                                                                          >  1:523942/1‑100 (MQ=255)

                     ggTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGCTTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGc                                                                            >  2:2197068/1‑97 (MQ=255)

                     ggTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTg                                                                         >  2:960696/1‑100 (MQ=255)

                     ggTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTg                                                                         >  1:184390/1‑100 (MQ=255)

                      gTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTgc                                                                        <  2:2293073/100‑1 (MQ=255)

                        naGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGATTAACGGCGTgagc                                                                      >  2:1279157/2‑100 (MQ=255)

                        cAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTgcgc                                                                      >  1:377379/1‑100 (MQ=255)

                        cAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTgcgc                                                                      >  1:2228287/1‑100 (MQ=255)

                        cAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTgcg                                                                       <  1:1903635/99‑1 (MQ=255)

                         aGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCt                                                                     <  1:174136/100‑1 (MQ=255)

                          gCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCtt                                                                    <  2:2246985/100‑1 (MQ=255)

                           cGATCCTTCCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCttt                                                                   <  1:57137/100‑1 (MQ=255)

                            gatgttACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACGCACGGCCTGACGAAGCGACTAACGGCGTGCGCtt                                                                    >  2:291779/6‑98 (MQ=255)

                            gATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTa                                                                  <  2:1710399/100‑1 (MQ=255)

                             aTCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTaa                                                                 >  2:1024486/1‑100 (MQ=255)

                              tCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAAt                                                                <  1:2268784/100‑1 (MQ=255)

                               ccTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATg                                                               <  1:261237/100‑1 (MQ=255)

                               ccTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATg                                                               >  1:1965550/1‑100 (MQ=255)

                                 tACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGAc                                                             <  1:1366250/100‑1 (MQ=255)

                                 tACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGAc                                                             <  1:2018081/100‑1 (MQ=255)

                                 tACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGAc                                                             >  1:915767/1‑100 (MQ=255)

                                  aCCGGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGAcc                                                            <  2:2603744/100‑1 (MQ=255)

                                    cTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTg                                                          >  1:1412669/1‑100 (MQ=255)

                                    cTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTg                                                          <  2:523938/100‑1 (MQ=255)

                                     tGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTCACGGCGTGCGCTTTAATGACCTg                                                          >  2:2096481/1‑99 (MQ=255)

                                      gggAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGtt                                                        >  2:887785/1‑100 (MQ=255)

                                       ggAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTg                                                       >  2:1912288/1‑100 (MQ=255)

                                         aaaaCCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGc                                                     <  2:2047447/100‑1 (MQ=255)

                                         aaaaCCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGc                                                     <  1:1053671/100‑1 (MQ=255)

                                          aaaCCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCg                                                    >  2:2615290/1‑100 (MQ=255)

                                            aCCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGAc                                                  >  1:2595952/1‑100 (MQ=255)

                                            aCCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGAc                                                  <  2:2510676/100‑1 (MQ=255)

                                              cACGTAGCAATATTCTCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGc                                                <  1:2716503/100‑1 (MQ=255)

                                                cGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTg                                              <  1:2011761/100‑1 (MQ=255)

                                                cGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTAACCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTg                                              >  2:449143/1‑100 (MQ=255)

                                                  tAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGaa                                            >  2:1695932/1‑100 (MQ=255)

                                                    gCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGaaaa                                          >  2:1565236/1‑100 (MQ=255)

                                                     cAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACTCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAAc                                         >  2:664250/1‑100 (MQ=255)

                                                     cAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAAc                                         >  1:697186/1‑100 (MQ=255)

                                                      aaTATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACa                                        <  1:344407/100‑1 (MQ=255)

                                                       atatTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACa                                        <  2:729377/99‑1 (MQ=255)

                                                       atatTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACAt                                       <  1:599430/100‑1 (MQ=255)

                                                         atTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTa                                     >  1:836619/1‑100 (MQ=255)

                                                         atTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTa                                     <  2:2005873/100‑1 (MQ=255)

                                                           tCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTAcc                                   <  1:2239150/100‑1 (MQ=255)

                                                            cGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCt                                  >  2:266856/1‑100 (MQ=255)

                                                            cGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCt                                  >  1:818334/1‑100 (MQ=255)

                                                            cGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCt                                  >  1:960813/1‑100 (MQ=255)

                                                             gCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTg                                 >  2:47801/1‑100 (MQ=255)

                                                              ccGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGc                                <  1:1024493/100‑1 (MQ=255)

                                                                ggggCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGccc                              <  2:1960182/100‑1 (MQ=255)

                                                                 gggCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCg                             <  2:2702201/100‑1 (MQ=255)

                                                                   ttccgatctgAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCa                           <  1:1443210/91‑1 (MQ=255)

                                                                   gCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCa                           <  1:1544708/100‑1 (MQ=255)

                                                                   gCTGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGAGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTAACTGCCCGCa                           >  2:598763/1‑100 (MQ=255)

                                                                     tGGATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGt                         >  2:642256/1‑100 (MQ=255)

                                                                      ggATTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTa                        <  1:2656347/100‑1 (MQ=255)

                                                                        aTTCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTAtt                      <  1:1347497/100‑1 (MQ=255)

                                                                         ttCGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATtg                     <  1:1976385/100‑1 (MQ=255)

                                                                           cGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATtgtg                   >  2:655632/1‑100 (MQ=255)

                                                                           cGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATtgtg                   <  1:739173/100‑1 (MQ=255)

                                                                           cGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATtgtg                   <  1:534903/100‑1 (MQ=255)

                                                                           cGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATtgtg                   <  2:1136218/100‑1 (MQ=255)

                                                                           cGAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATtgtg                   >  1:1788476/1‑100 (MQ=255)

                                                                            naaGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCaagatcggaa                  >  2:1443200/2‑91 (MQ=255)

                                                                            gAAGTGAGTACTTACCCGTGACATGGCGAAGCGACTAACGTCGTGCGCTTTAATGGCCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGGATGgtgt                  >  2:1196759/1‑100 (MQ=255)

                                                                            gAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTgtgt                  >  2:2508946/1‑100 (MQ=255)

                                                                            gAAGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTgtgt                  >  1:1318516/1‑100 (MQ=255)

                                                                             aaGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGtt                 <  2:258273/100‑1 (MQ=255)

                                                                             aaGTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGtt                 >  2:795691/1‑100 (MQ=255)

                                                                               gTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTtgc               >  1:1692750/1‑100 (MQ=255)

                                                                               gTGAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTtgc               >  1:1407360/1‑100 (MQ=255)

                                                                                tGAGTACTTACCCCTGGCATGACGAAGCGACTAACGACGTGCGCTTTAATGACCTGTTGGCGAGGCTGAAAACATTACCTGCCCGCCGCATTGTGTtgct              >  1:1948844/1‑100 (MQ=255)

                                                                                 gAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTtgctg             >  2:255905/1‑100 (MQ=255)

                                                                                 gAGTACTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTtgctg             >  2:19771/1‑100 (MQ=255)

                                                                                     aCTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATc         <  2:2017787/100‑1 (MQ=255)

                                                                                     aCTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATc         >  1:1392369/1‑100 (MQ=255)

                                                                                      cTTACCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATc         <  1:2096498/99‑1 (MQ=255)

                                                                                         aCCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATg     <  2:1412660/100‑1 (MQ=255)

                                                                                         aCCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATg     >  2:2579489/1‑100 (MQ=255)

                                                                                         aCCCCTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATg     >  2:981010/1‑100 (MQ=255)

                                                                                          ccccTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGt    >  2:863552/1‑100 (MQ=255)

                                                                                           cccTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGtt   <  2:597622/100‑1 (MQ=255)

                                                                                            ccTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTg  >  2:708787/1‑100 (MQ=255)

                                                                                            ccTGGCATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTg  >  2:2079860/1‑100 (MQ=255)

CTTCCCGGAATCAGGCGTCTGGGTCAGCGATCCTACCTGGGAAAACCACGTAGCAATATTCGCCGGGGCTGGATTCGAAGTGAGTACTTACCCCTGGTATGACGAAGCGACTAACGGCGTGCGCTTTAATGACCTGTTGGCGACGCTGAAAACATTACCTGCCCGCAGTATTGTGTTGCTGCATCCATGTTG  >  NC_000913/4267467‑4267658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`