Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,679,430 Δ1 bp 23.7% intergenic (‑63/+34) yphF ← / ← yphG putative sugar ABC transporter periplasmic binding protein/DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,679,4300G.23.7% 319.6 / 105.6 118intergenic (‑63/+34)yphF/yphGputative sugar ABC transporter periplasmic binding protein/DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (51/39);  new base . (15/13);  total (66/52)
Fisher's exact test for biased strand distribution p-value = 8.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 121 total aligned reads displayed.

ATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAA  >  NC_000913/2679331‑2679524
                                                                                                   |                                                                                               
ataataGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAg                                                                                                 >  2:2772315/1‑100 (MQ=255)
 taataGATTACGGGTTGTTCTCATTTTTGTAGGCATGGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAgc                                                                                                >  2:2053717/1‑100 (MQ=255)
   ataGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAgcgc                                                                                              >  2:46170/1‑100 (MQ=255)
     ataTTACTGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTAGTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGcgcg                                                                                             <  1:358615/97‑1 (MQ=255)
        ttACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGcc                                                                                        <  2:720038/100‑1 (MQ=255)
        ttACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTCTAGTGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGcc                                                                                        <  1:2288261/100‑1 (MQ=255)
          aCGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGc                                                                                      <  2:2473238/100‑1 (MQ=255)
          aCGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGc                                                                                      <  2:2643440/100‑1 (MQ=255)
           cGGGTTGTTCTCATTTTTGTAGGCATAGTGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCt                                                                                     <  1:646837/100‑1 (MQ=255)
            gggTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTc                                                                                    <  2:456522/100‑1 (MQ=255)
            gggTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTc                                                                                    <  2:1948360/100‑1 (MQ=255)
             ttccgatctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCa                                                                                   <  1:494635/94‑1 (MQ=255)
             ttccgatctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCa                                                                                   <  1:494645/94‑1 (MQ=255)
             ggTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCa                                                                                   <  2:2556269/100‑1 (MQ=255)
             ggTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCa                                                                                   >  2:107158/1‑100 (MQ=255)
             ggTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCa                                                                                   <  1:2900234/100‑1 (MQ=255)
              gttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACATCGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCat                                                                                  <  1:2011903/100‑2 (MQ=255)
              gttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCat                                                                                  <  1:2447584/100‑2 (MQ=255)
              gttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCat                                                                                  <  2:2222116/100‑2 (MQ=255)
               ttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCaaa                                                                                 <  2:1564071/100‑1 (MQ=255)
               ttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCatc                                                                                 <  1:516696/100‑3 (MQ=255)
               ccgatctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCatc                                                                                 <  1:257914/96‑3 (MQ=255)
                tgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACTCGCTGCCGCTCatca                                                                                <  1:517898/100‑4 (MQ=255)
                tgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCatca                                                                                <  2:785316/100‑4 (MQ=39)
                 gttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTACTTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAata                                                                               >  2:2453761/1‑100 (MQ=255)
                 gttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAata                                                                               >  2:2125092/1‑100 (MQ=255)
                   tctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATAtt                                                                             <  2:1343239/100‑1 (MQ=255)
                    ctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTa                                                                            >  2:2429287/1‑100 (MQ=255)
                      cATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACa                                                                          >  2:228457/1‑100 (MQ=255)
                      cATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCatcagatcgg                                                                          >  2:257915/1‑91 (MQ=38)
                      cATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCaagatcggaa                                                                          >  2:494638/1‑92 (MQ=255)
                      cATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCACGCGCTGCCGCTCaagatcggaa                                                                          >  2:494648/1‑92 (MQ=255)
                       aTTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCACTCAAATATTACAg                                                                         <  2:2334897/100‑1 (MQ=255)
                          tttGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTa                                                                      >  1:2657046/1‑100 (MQ=255)
                                 gCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGt                                                               <  2:2197801/100‑1 (MQ=255)
                                 gCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAAGAggg                                                                >  1:2306960/1‑99 (MQ=255)
                                  cATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGtt                                                              >  2:400268/1‑100 (MQ=255)
                                   aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGttt                                                             <  1:937193/100‑1 (MQ=255)
                                      gagCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTTTTTTTTTTTTCAGCGCGTGCCGCTCAAATATTACATTTAATAGGGTTTAg                                                           >  1:2332145/1‑99 (MQ=255)
                                      gagCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGt                                                          <  2:726297/100‑1 (MQ=255)
                                      gagCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGt                                                          >  2:922911/1‑100 (MQ=255)
                                       agCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTg                                                         <  1:307305/100‑1 (MQ=255)
                                        gCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGa                                                        >  1:1085555/1‑100 (MQ=255)
                                           ttctGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAAcg                                                     <  1:709605/98‑1 (MQ=255)
                                           tcctGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAAcg                                                     >  1:1326845/1‑100 (MQ=255)
                                            cctGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAAcgc                                                    <  1:2446036/100‑1 (MQ=255)
                                            cctGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAAcgc                                                    >  2:1842114/1‑100 (MQ=255)
                                              tGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCgg                                                  >  1:2556545/1‑100 (MQ=255)
                                               gTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGt                                                 >  2:750997/1‑100 (MQ=255)
                                                tAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGtt                                                >  2:1116287/1‑100 (MQ=255)
                                                tAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGtt                                                <  2:2472413/100‑1 (MQ=255)
                                                tAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGtt                                                <  1:467902/100‑1 (MQ=255)
                                                tAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTAAGTGAAc                                                      >  1:938709/1‑94 (MQ=255)
                                                   ggTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaa                                             <  2:873887/100‑1 (MQ=255)
                                                    gTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaaa                                            >  2:1520310/1‑100 (MQ=255)
                                                     tttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaaaa                                           <  2:657108/100‑1 (MQ=255)
                                                     tttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaaa                                            <  1:1437091/99‑1 (MQ=255)
                                                       tttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAGTAGGGTTTAGTGAACGCGGTTAAAAATa                                         >  1:1027500/1‑100 (MQ=255)
                                                       tttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATa                                         >  1:2179166/1‑100 (MQ=255)
                                                         tattaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATaaa                                       <  2:1365599/100‑1 (MQ=255)
                                                         tattaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATaaa                                       >  1:2759672/1‑100 (MQ=255)
                                                            taACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAgg                                    >  2:193250/1‑100 (MQ=255)
                                                             aacaacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGc                                   >  1:623927/1‑100 (MQ=255)
                                                               caacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCAt                                 >  1:264209/1‑100 (MQ=255)
                                                                 acGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCa                               >  1:1811662/1‑100 (MQ=255)
                                                                    gCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACgg                            >  1:1176657/1‑100 (MQ=255)
                                                                       tATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtgg                         >  1:406750/1‑100 (MQ=255)
                                                                        aTTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtggt                        <  1:2795641/100‑1 (MQ=255)
                                                                         ttCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGgtggtg                       <  2:2586052/100‑1 (MQ=255)
                                                                          tCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAATTATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGa                      >  1:1811644/1‑100 (MQ=255)
                                                                          tCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGa                      >  1:2139447/1‑100 (MQ=255)
                                                                          tCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGa                      >  2:221207/1‑100 (MQ=255)
                                                                           cTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGaa                     >  2:1396092/1‑100 (MQ=255)
                                                                           cTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGaa                     <  2:1013897/100‑1 (MQ=255)
                                                                              attattTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGaataa                  >  1:612650/1‑100 (MQ=255)
                                                                                tattTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATg                <  2:2825465/100‑1 (MQ=255)
                                                                                ctgtaatatttgaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATg                >  2:1699934/11‑100 (MQ=37)
                                                                                 attTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGc               >  1:2212481/1‑100 (MQ=255)
                                                                                 attTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGc               <  2:1176662/100‑1 (MQ=255)
                                                                                  gtaatatttgaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGcc              >  2:1954240/9‑100 (MQ=37)
                                                                                   tttGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCg             >  1:2064728/1‑100 (MQ=255)
                                                                                     atatttgaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAACATAAAAGGCATCACGGAGGTGAATAATGCCGc            >  2:100038/6‑99 (MQ=37)
                                                                                     atatttgaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGc            >  1:618286/6‑99 (MQ=38)
                                                                                        tttgaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTc        >  1:319243/3‑100 (MQ=255)
                                                                                         ttgaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCg       >  1:2614024/2‑100 (MQ=255)
                                                                                         ttgaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCg       >  2:517164/2‑100 (MQ=255)
                                                                                         ttgaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCg       >  1:1879586/2‑100 (MQ=255)
                                                                                         atgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTc        <  2:1274024/99‑1 (MQ=255)
                                                                                         atgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCg       <  2:1844961/100‑1 (MQ=255)
                                                                                         atgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCg       >  1:1725084/1‑100 (MQ=255)
                                                                                         atgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCg       >  1:1648856/1‑100 (MQ=255)
                                                                                         atgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCg       >  2:1142877/1‑100 (MQ=255)
                                                                                          tgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCgg      <  2:581975/100‑1 (MQ=255)
                                                                                          tgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTAAATAATGCCGCTTTCgg      >  1:1565168/1‑100 (MQ=255)
                                                                                          tgaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGCACGCGGTTAAAAATAAAAGGCATCACGGTGGTCAATAATGCCGCTTTc        >  1:196630/1‑98 (MQ=255)
                                                                                          tgaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCgg      >  2:1510980/1‑100 (MQ=255)
                                                                                           gaGCGGCACGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGAATCACGGAGGTGAATAATGCCGCTTTCGGc     >  2:2907704/1‑100 (MQ=255)
                                                                                            aGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGcc    >  2:2748952/1‑100 (MQ=255)
                                                                                            aGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGcc    >  2:2493351/1‑100 (MQ=255)
                                                                                              cGGCAGCGCGTGCCGCTCAAATATTACGTTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCaa  <  2:673652/100‑1 (MQ=255)
                                                                                                   |                                                                                               
ATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAA  >  NC_000913/2679331‑2679524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: