Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 220,849 (T)5→4 100% coding (80/816 nt) metQ ← DL‑methionine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913220,8450T.86.1% 325.6 / 53.7 101coding (84/816 nt)metQDL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (9/4);  new base . (40/47);  total (49/52)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 109 total aligned reads displayed.

ACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCCTTC  >  NC_000913/220747‑220941
                                                                                                  |                                                                                                
aCGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCtt                                                                                                 <  2:1204412/100‑1 (MQ=255)
aCGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCtt                                                                                                 >  2:2310339/1‑100 (MQ=255)
 cGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttt                                                                                                <  1:2590454/100‑1 (MQ=255)
  gTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCtttt                                                                                               <  2:1752008/100‑1 (MQ=255)
    ccAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttttca                                                                                             >  1:1523411/1‑98 (MQ=255)
     cAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttttcat                                                                                            >  2:2752011/1‑97 (MQ=255)
      aGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttttcatc                                                                                           >  1:2540596/1‑96 (MQ=255)
         ccATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCt                                                                                        >  2:2142550/1‑100 (MQ=255)
         ccATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTg                                                                                       <  1:1929592/100‑1 (MQ=255)
          cttatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGa                                                                                      <  2:216720/98‑1 (MQ=255)
            tatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGaag                                                                                    >  1:216719/1‑98 (MQ=255)
            tatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGAcc                                                                                    <  2:2231205/100‑1 (MQ=255)
              tttGTTTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGTTGTTAGGTACCAACAATCACGCCGACTTTAATTTGGTTTAGATCTTTTCATCCTGACCGc                                                                                  <  1:843347/100‑1 (MQ=255)
               ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCa                                                                                 <  2:544406/100‑1 (MQ=255)
                tGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAg                                                                                <  2:177129/100‑1 (MQ=255)
                tGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAg                                                                                <  2:420682/100‑1 (MQ=255)
                 gTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGc                                                                               >  1:1413703/1‑100 (MQ=255)
                     ttCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAc                                                                           >  1:2303206/1‑100 (MQ=255)
                      tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAcc                                                                          <  1:2841535/100‑1 (MQ=255)
                      tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAcc                                                                          <  1:480741/100‑1 (MQ=255)
                      tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAcc                                                                          >  2:1863903/1‑100 (MQ=255)
                      tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAcc                                                                          <  1:688008/100‑1 (MQ=255)
                      tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAcc                                                                          >  2:1083502/1‑100 (MQ=255)
                      tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAcc                                                                          >  1:2353879/1‑100 (MQ=255)
                       cGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAcca                                                                         >  2:2279764/1‑100 (MQ=255)
                       cGCAAATTTCTGCGCGCCTTCTGCAATCTGCTGTTCCGCACCGACAATCACGCCAACTTTAATGTGGTTTGGATCTTTTCGTCCTGACAGCAGCCTa                                                                            <  1:355995/97‑1 (MQ=255)
                        gCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTAcca                                                                         >  1:542831/1‑100 (MQ=255)
                        gCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccag                                                                        <  2:822945/100‑1 (MQ=255)
                        gCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccag                                                                        >  2:417801/1‑100 (MQ=255)
                        gCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccag                                                                        >  2:2623209/1‑100 (MQ=255)
                            cTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagtgcc                                                                    <  2:2219189/100‑1 (MQ=255)
                              ttCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagtgccag                                                                  <  1:500930/100‑1 (MQ=255)
                              ttCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagtgccag                                                                  >  1:2236765/1‑100 (MQ=255)
                               tCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTAccagtgccag                                                                  <  1:2188536/100‑1 (MQ=255)
                               tCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTAccagtgccagt                                                                 <  1:1033270/100‑1 (MQ=255)
                                cTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTAccagtgccag                                                                  <  2:1062070/99‑1 (MQ=255)
                                  gcgcGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTCACCGCACCCTACCAGTGCCAGTGAt                                                              >  1:1268412/1‑100 (MQ=255)
                                   cgcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATc                                                             >  1:2345888/1‑100 (MQ=255)
                                    gcgACTTCTGCAAACTGCTGTTGGGCACCACCAATCACGCCGACTTTAACGTGGTTTGGATGTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTAAtac                                                            <  1:2689039/100‑3 (MQ=255)
                                     cgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCg                                                           <  2:2454218/100‑1 (MQ=255)
                                       aCTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGAt                                                         <  1:1396599/100‑1 (MQ=255)
                                         ttCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCa                                                       <  2:1629548/100‑1 (MQ=255)
                                          tCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCa                                                       >  2:1716331/1‑99 (MQ=255)
                                          tCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAg                                                      >  2:394678/1‑100 (MQ=255)
                                           cTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAgg                                                     <  2:2078644/100‑1 (MQ=255)
                                            tGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAggg                                                    <  2:2029702/100‑1 (MQ=255)
                                               aaCCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTc                                                 <  2:680611/100‑1 (MQ=255)
                                               aaCCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTc                                                 <  2:1154558/100‑1 (MQ=255)
                                                aCCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTcc                                                >  2:2307469/1‑100 (MQ=255)
                                                aCCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTcc                                                <  2:1494561/100‑1 (MQ=255)
                                                  ctgctgTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCa                                              <  2:2638470/100‑1 (MQ=255)
                                                  ctgctgTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCa                                              >  2:2847010/1‑100 (MQ=255)
                                                    gctgTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACt                                            >  2:117459/1‑100 (MQ=255)
                                                      tgTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGc                                          >  1:107077/1‑100 (MQ=255)
                                                      tgTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGc                                          >  2:775700/1‑100 (MQ=255)
                                                       gTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGcc                                         >  2:2685087/1‑100 (MQ=255)
                                                        ttCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCg                                        <  2:1374832/100‑1 (MQ=255)
                                                        ttCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCg                                        <  2:2245406/100‑1 (MQ=255)
                                                        ttCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCg                                        <  1:2737762/100‑1 (MQ=255)
                                                           ggCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCaa                                     >  2:1485225/1‑100 (MQ=255)
                                                            gCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCaaa                                    >  2:1509511/1‑100 (MQ=255)
                                                             cACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAg                                   >  2:186622/1‑100 (MQ=255)
                                                                caacaaTCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGt                                 >  1:2130827/1‑100 (MQ=255)
                                                                caacaaTCACGCCGACTTTAATGTGGTTTGGATCTTTTTATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAgg                                  >  1:895385/1‑98 (MQ=255)
                                                                caacaaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGtt                                >  1:618966/1‑100 (MQ=255)
                                                                 aacaaTCACGCCGACTTTAATGTGGTTTGGCTCTTTTCATCCTTACCGCAGCCTCCCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGttt                               <  1:1088736/100‑1 (MQ=255)
                                                                 aacaaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGttt                               >  2:1736144/1‑100 (MQ=255)
                                                                  acaaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGtttt                              <  2:1163820/100‑1 (MQ=255)
                                                                  acaaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGtttt                              <  1:1934397/100‑1 (MQ=255)
                                                                   caaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttg                             >  2:1791495/1‑100 (MQ=255)
                                                                    aaTCACGCCTACTTTAATGTTGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttg                             <  2:241291/99‑1 (MQ=255)
                                                                    aaTCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttga                            <  1:1122615/100‑1 (MQ=255)
                                                                      tCACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaat                          <  2:1180527/100‑1 (MQ=255)
                                                                       cACGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaatt                         <  1:1083498/100‑1 (MQ=255)
                                                                        aCGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaatt                         >  2:604279/1‑100 (MQ=255)
                                                                        aCGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaattt                        >  1:2697698/1‑100 (MQ=255)
                                                                        aCGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaattt                        <  1:1521410/100‑1 (MQ=255)
                                                                         cGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaatttg                       <  1:2507169/100‑1 (MQ=255)
                                                                         cGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaattt                        <  1:2715650/99‑1 (MQ=255)
                                                                         cGCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaatt                         >  1:1268641/1‑98 (MQ=255)
                                                                          gCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaatttg                       <  1:1178208/100‑1 (MQ=255)
                                                                          gCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaatttga                      <  2:2601015/100‑1 (MQ=255)
                                                                          gCCGACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaatttga                      >  1:93875/1‑100 (MQ=255)
                                                                           ccGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTtttgaatttga                      >  2:2120209/1‑100 (MQ=255)
                                                                             gACTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGTCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACg                   <  2:2724914/100‑1 (MQ=255)
                                                                               cTTTAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGcc                 >  1:1162075/1‑100 (MQ=255)
                                                                                  tAATGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATa              >  2:671652/1‑100 (MQ=255)
                                                                                    aTGTGGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGCTCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAACTCAAACGCCATAcc            >  1:775595/1‑100 (MQ=255)
                                                                                     tgtgGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCt           >  2:62806/1‑100 (MQ=255)
                                                                                     tgtgGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCt           >  2:1358924/1‑100 (MQ=255)
                                                                                     tgtgGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCt           >  2:1229409/1‑100 (MQ=255)
                                                                                       tgGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTa         <  2:2653806/100‑1 (MQ=255)
                                                                                       tgGTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTa         >  1:1654844/1‑100 (MQ=255)
                                                                                        ggTTTGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTAt        <  2:2624590/100‑1 (MQ=255)
                                                                                          tttGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTc      >  1:1122657/1‑100 (MQ=255)
                                                                                          tttGGATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTc      >  1:1692422/1‑100 (MQ=255)
                                                                                             ggATCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCct    >  2:75214/1‑100 (MQ=255)
                                                                                             ggATCTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCctt   >  1:1622424/1‑100 (MQ=255)
                                                                                               aTCTTTTTCATCCTGACCGCAGCCTCCCAGTGCGAGTGATCCGATCAGGGGTCCCACTGCCGCAAAGGTTTTGAATCTGAACGCCATACCTTATTCcttc  <  1:1727550/100‑1 (MQ=255)
                                                                                               aTCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCcttc  >  1:2893470/1‑100 (MQ=255)
                                                                                                  |                                                                                                
ACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCGCAGCCTACCAGTGCCAGTGATCCGATCAGGGCTCCCACTGCCGCAAAGGTTTTGAATTTGAACGCCATACCTTATTCCTTC  >  NC_000913/220747‑220941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: