Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,549,792 (C)6→5 100% coding (279/2085 nt) malQ ← 4‑alpha‑glucanotransferase (amylomaltase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,549,7870C.89.0% 371.3 / 41.1 100coding (284/2085 nt)malQ4‑alpha‑glucanotransferase (amylomaltase)
Reads supporting (aligned to +/- strand):  ref base C (2/9);  new base . (52/37);  total (54/46)
Fisher's exact test for biased strand distribution p-value = 2.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-01

Only 100 of 117 total aligned reads displayed.

GGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACCACCATCGGCATTTTTTTG  >  NC_000913/3549688‑3549882
                                                                                                   |                                                                                               
ggggCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGc                                                                                                 <  1:2269838/100‑1 (MQ=255)
ggggCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGc                                                                                                 >  2:1352579/1‑100 (MQ=255)
ggggCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGc                                                                                                 >  2:1986356/1‑100 (MQ=255)
 gggCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGCTTGAACGCTTTGcc                                                                                                >  1:958672/1‑100 (MQ=255)
 gggCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGcc                                                                                                >  1:1414167/1‑100 (MQ=255)
 gggCGACAATCACCCGGCAATGCGCGCGCGGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGcc                                                                                                <  1:2795200/100‑1 (MQ=255)
  ggCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGccc                                                                                               >  1:464699/1‑100 (MQ=255)
   gCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGcccc                                                                                              <  1:1035861/100‑1 (MQ=255)
    cGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGccccc                                                                                             >  1:2234635/1‑100 (MQ=255)
       cAATCACCCGGCAATGTGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGcccccgtt                                                                                          >  2:251678/1‑97 (MQ=255)
       cAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGcccccgtt                                                                                          <  1:2526503/100‑4 (MQ=255)
       cAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGcccccgtt                                                                                          >  2:937619/1‑97 (MQ=255)
       cAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGcccccgtt                                                                                          >  2:288069/1‑97 (MQ=255)
       cAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGt                                                                                          <  2:2476176/100‑1 (MQ=255)
        aaTCACCCGGCAATGCGCTCGCTGGTCGTCCTGTGTGAGTGCCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGcccccgtta                                                                                         <  1:151690/100‑5 (MQ=255)
        aaTCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGtt                                                                                         <  2:287744/100‑1 (MQ=255)
        aaTCACCCGGCAATGCGCGCGCTGGTCGGCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCTGTAGATTGAACGCTTTGcccccgtta                                                                                         <  1:151677/100‑5 (MQ=255)
            aCCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACAt                                                                                     <  1:2276712/99‑1 (MQ=255)
            aCCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATg                                                                                    >  1:928579/1‑100 (MQ=255)
             cccGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACAt                                                                                     >  1:738676/1‑98 (MQ=255)
               cGGCAATGCGCGCGCTGGTCGGCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGcc                                                                                 <  2:1139925/100‑1 (MQ=255)
                 gCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCtt                                                                               >  2:2685378/1‑100 (MQ=255)
                  cAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCttt                                                                              >  2:2747400/1‑100 (MQ=255)
                  cAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCttt                                                                              >  2:2910144/1‑100 (MQ=255)
                    aTGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGt                                                                            <  1:2068921/100‑1 (MQ=255)
                    aTGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGt                                                                            <  1:1802897/100‑1 (MQ=255)
                     tGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTa                                                                           <  2:2089622/100‑1 (MQ=255)
                      gcgcgcgcTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTAc                                                                          <  2:2326142/100‑1 (MQ=255)
                       cgcgcgcTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACt                                                                         <  1:1778269/100‑1 (MQ=255)
                       cgcgcgcTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACt                                                                         >  2:431057/1‑100 (MQ=255)
                         cgcgcTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTg                                                                        <  1:104858/100‑1 (MQ=255)
                         cgcgcTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGc                                                                       >  2:1813089/1‑100 (MQ=255)
                          gcgcTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCg                                                                      >  1:2223247/1‑100 (MQ=255)
                          gcgcTGGTCGTCCTGGGTGAGTGCCAGCGTGTGACAACCTTCCCGCAGCTTCGTCGGTAGATAGAACGCTTAGCCCCCGTTACATGGCCTTTGTACTg                                                                        <  1:2323688/98‑1 (MQ=255)
                            gcTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGtt                                                                    <  1:23935/100‑1 (MQ=255)
                             cTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTc                                                                   >  1:1565169/1‑100 (MQ=255)
                              tGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTcc                                                                  >  2:2220334/1‑100 (MQ=255)
                              tGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTcc                                                                  <  2:1695992/100‑1 (MQ=255)
                               ggTCGTCCTGGGTGAGTGTCAGCGTGTTATATCCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGc                                                                       <  1:633256/94‑1 (MQ=255)
                               ggTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCct                                                                 >  2:2403775/1‑100 (MQ=255)
                               ggTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCct                                                                 <  2:2363050/100‑1 (MQ=255)
                                gtcgtcCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCctt                                                                >  1:1660038/1‑100 (MQ=255)
                                  cgtcCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCcttc                                                               <  2:1679831/100‑1 (MQ=255)
                                   gtcCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCcttct                                                              <  1:491189/100‑1 (MQ=255)
                                   gtcCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCcttctt                                                             >  2:390007/1‑100 (MQ=255)
                                     ccTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCg                                                           >  1:1461405/1‑100 (MQ=255)
                                     ccTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCCTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCg                                                           >  1:1590345/1‑100 (MQ=255)
                                      cTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCgg                                                          <  1:1658797/100‑1 (MQ=255)
                                      cTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCgg                                                          >  2:462413/1‑100 (MQ=255)
                                       tGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCggt                                                         >  2:883617/1‑100 (MQ=255)
                                        gggTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCggtg                                                        <  1:2632569/100‑1 (MQ=255)
                                        gggTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCggtg                                                        >  2:541731/1‑100 (MQ=255)
                                         ggTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCggtgg                                                       <  2:2094386/100‑1 (MQ=255)
                                         ggTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCggtgg                                                       <  2:1755692/100‑1 (MQ=255)
                                          gTGAGTGTCAGCGTGTGATAACCTTCCGGCATCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCggtgg                                                       <  1:1677876/99‑1 (MQ=255)
                                          gTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCggtgg                                                       <  2:2243082/100‑1 (MQ=255)
                                          gTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCggtggt                                                      >  2:2617645/1‑100 (MQ=255)
                                           tGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTc                                                     >  1:1514659/1‑100 (MQ=255)
                                           tGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTc                                                     <  2:871804/100‑1 (MQ=255)
                                            gcgtgtCAGCGTGTGAGACCCTTCCGGCAGCTTCTTCGCTTGAGTCAGCGCTGTCGCCGCGTTGCATGCCCTGTGTACTGCGCTCGTCCTTCggtgg                                                       <  1:2814691/95‑1 (MQ=255)
                                              gtgtCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTcagc                                                  <  2:1606216/100‑1 (MQ=255)
                                                gtCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTcagcag                                                <  2:1853019/100‑1 (MQ=255)
                                                 tCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCagcagc                                               >  2:1802007/1‑100 (MQ=255)
                                                  ctgggtgtCATGACTTTCCGTCAGCTTGGTCGGTAGTTTGAGCGGTTTGCCCGCTTGACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTca                                                    <  1:1069235/90‑1 (MQ=255)
                                                   aGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGcagcca                                             >  2:1943675/1‑100 (MQ=255)
                                                   aGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGcagcca                                             >  2:1681401/1‑100 (MQ=255)
                                                    gCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGcagccag                                            >  1:1111263/1‑100 (MQ=255)
                                                     cGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGcagccagc                                           <  2:1575/100‑1 (MQ=255)
                                                       tgtgATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCta                                         >  1:2141591/1‑100 (MQ=255)
                                                        gtgATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCtat                                        >  1:1251277/1‑100 (MQ=255)
                                                         tgATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCtata                                       <  2:1414172/100‑1 (MQ=255)
                                                            tAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTc                                    >  2:1041050/1‑100 (MQ=255)
                                                            tAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTc                                    >  1:1423134/1‑100 (MQ=255)
                                                             aaCCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTcg                                   >  1:2167582/1‑100 (MQ=255)
                                                              aCCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTcgc                                  >  1:357682/1‑100 (MQ=255)
                                                               ccTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTcgcc                                 >  2:2567031/1‑100 (MQ=255)
                                                               ccTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTcgcc                                 >  2:2805489/1‑100 (MQ=255)
                                                                   ccGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTg                             <  2:67628/100‑1 (MQ=255)
                                                                      gCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGccc                          <  2:1991909/100‑1 (MQ=255)
                                                                      gCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGccc                          <  2:1271292/100‑1 (MQ=255)
                                                                       cAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGccc                          >  1:796747/1‑100 (MQ=255)
                                                                         gCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTcc                       >  2:2184650/1‑100 (MQ=255)
                                                                         gCTTCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTcc                       >  1:2749183/1‑100 (MQ=255)
                                                                          cTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTcc                       >  1:2664996/1‑100 (MQ=255)
                                                                           ttCGTCGGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTccac                     >  2:1119505/1‑100 (MQ=255)
                                                                             cgtcgGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTccacca                   <  1:1329530/100‑1 (MQ=255)
                                                                                cgGTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTccaccacca                >  2:2902815/1‑100 (MQ=255)
                                                                                  gTAGATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACCACCATc              <  2:2066523/100‑1 (MQ=255)
                                                                                    aGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACCACCATCg             <  2:707639/100‑1 (MQ=255)
                                                                                    aGATGGAGCGCTTTGTCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACTACCATCg             <  1:1624309/99‑1 (MQ=255)
                                                                                     gATTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACCACCATCGGc           <  2:419758/100‑1 (MQ=255)
                                                                                      aTTGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACCACCATCGGCa          >  2:867361/1‑100 (MQ=255)
                                                                                       ttGAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACCACCATCGGCAt         >  2:857246/1‑100 (MQ=255)
                                                                                         gAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACCACCATCGGCAttt       >  2:534845/1‑100 (MQ=255)
                                                                                         gAACGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACCACCATCGGCAttt       >  2:761788/1‑100 (MQ=255)
                                                                                            cGCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACCACCATCGGCAtttttt    <  2:1783010/100‑1 (MQ=255)
                                                                                             gCTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACCACCATCGGCAttttttt   <  2:1097526/100‑1 (MQ=255)
                                                                                              cTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACCACCATCGGCATTTTTTTg  >  2:2000181/1‑100 (MQ=255)
                                                                                              cTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACCACCATCGGCATTTTTTTg  >  2:797501/1‑100 (MQ=255)
                                                                                              cTTTGCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACCACCATCGGCATTTTTTTg  >  1:2258841/1‑100 (MQ=255)
                                                                                                   |                                                                                               
GGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCCCTCCACCACCATCGGCATTTTTTTG  >  NC_000913/3549688‑3549882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: