Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,520,500 Δ1 bp 16.3% intergenic (‑176/+171) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,5000A.16.3% 293.5 / 55.0 104intergenic (‑176/+171)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Reads supporting (aligned to +/- strand):  ref base A (47/40);  new base . (7/10);  total (54/50)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.02e-01

Only 100 of 107 total aligned reads displayed.

GGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGC  >  NC_000913/4520402‑4520599
                                                                                                  |                                                                                                    
ggCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGAc                                                                                                     <  1:1226403/100‑1 (MQ=255)
ggCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGAc                                                                                                     <  2:1943529/100‑1 (MQ=255)
 gCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACa                                                                                                    <  2:2634312/100‑1 (MQ=255)
 gCATAATGTTATACAAATATTTTTGTCTTTTACACGAAATAATCCATCAGAAAATTTGAGCGATCGCCCGTTGTTCCCCCCTCCTGTAAAGCCGGAGACa                                                                                                    <  1:1390145/100‑1 (MQ=255)
  cATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAt                                                                                                   <  2:1335522/100‑1 (MQ=255)
  cATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAt                                                                                                   >  1:1119860/1‑100 (MQ=255)
  cATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAt                                                                                                   >  2:1037381/1‑100 (MQ=255)
    tGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGc                                                                                                >  1:2164836/1‑100 (MQ=255)
         ttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCTCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCgg                                                                                           <  2:1272504/100‑1 (MQ=38)
         ttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCgg                                                                                           >  2:1865405/1‑100 (MQ=255)
         ttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCgg                                                                                           <  1:796386/100‑1 (MQ=39)
          tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGc                                                                                          <  2:412826/100‑1 (MQ=255)
          tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGc                                                                                          <  2:2686090/100‑1 (MQ=39)
          tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGc                                                                                          <  2:2338252/100‑1 (MQ=39)
           ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCt                                                                                         <  2:208636/100‑1 (MQ=255)
             aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCttt                                                                                       <  2:1716603/100‑1 (MQ=38)
              cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTa                                                                                      <  2:2502630/100‑1 (MQ=38)
               aGATATTTTATTCTTTTACATGAAATAACCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTAc                                                                                     <  1:1122089/100‑1 (MQ=255)
               aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCTAGCTGTTCCCCCATACTGTAAAACCGGAGACATGCTCCGGCTTTa                                                                                      >  1:1352346/1‑99 (MQ=255)
               aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTAc                                                                                     <  2:1401147/100‑1 (MQ=255)
               aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTAc                                                                                     <  2:233231/100‑1 (MQ=38)
                gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTTCCCCATACTACAAAGCCGGAGACAGGCTCCgg                                                                                           >  1:2523651/1‑93 (MQ=255)
                gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACa                                                                                    <  2:125419/100‑1 (MQ=255)
                gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACa                                                                                    <  1:691585/100‑1 (MQ=255)
                gATATTTTATTCTCTTACATGAAATAAACCAACATACCATTTAAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACa                                                                                    >  2:1967624/1‑100 (MQ=255)
                 atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAg                                                                                   <  2:2451909/100‑1 (MQ=38)
                    ttttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTAt                                                                                <  2:253695/100‑1 (MQ=255)
                       tATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTAATCCCACCTACTGAAAAGCCGGAGAAATACTACGGCAAAACAGTATTAc                                                                             >  2:1696358/1‑100 (MQ=255)
                       tATTCGTTTACATGAGATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCTCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTAc                                                                             <  1:202819/100‑1 (MQ=255)
                        aTTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACt                                                                            <  1:2549540/100‑1 (MQ=255)
                        aTTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTAtgggg                                                                            <  2:457403/100‑5 (MQ=25)
                         ttCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTg                                                                           <  2:2904495/100‑1 (MQ=255)
                           cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAACGTCGGAGACATGCTCCGGCTTTACAGTATTACTgag                                                                         >  1:1433283/1‑100 (MQ=255)
                           cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTgag                                                                         >  1:1631650/1‑100 (MQ=255)
                           cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTgag                                                                         <  2:2123667/100‑1 (MQ=255)
                           cTTTTACATGAAATAAACCAGCATACCATTTGAGAGATCGCCAGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACt                                                                            >  2:2703473/1‑97 (MQ=255)
                           cTTTTACATCATATACACCAGCATTCCATTTGATCGCTCGCCCTCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTGACAGTATTa                                                                              <  1:2165717/95‑1 (MQ=37)
                            ttttACATGAAATAAACCAGCATACCATTTGTGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTgaga                                                                        <  2:2811701/100‑1 (MQ=255)
                             tttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGaa                                                                       >  1:154420/1‑100 (MQ=255)
                             tttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGaa                                                                       <  1:90219/100‑1 (MQ=255)
                              ttACATGAAATAAACCAGCATACCATTTGAGCGATCGACCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAAt                                                                      >  1:215349/1‑100 (MQ=255)
                               tACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATa                                                                     >  2:2681518/1‑100 (MQ=255)
                                 cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATaca                                                                   >  1:384953/1‑100 (MQ=255)
                                  aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATacac                                                                  >  2:1277405/1‑100 (MQ=255)
                                   tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                 >  2:508579/1‑100 (MQ=255)
                                   tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                 <  2:1107846/100‑1 (MQ=255)
                                      aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCAt                                                              >  1:615331/1‑100 (MQ=255)
                                       aTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGACTTTACAGTATTACTGAGAATACAccc                                                                <  1:1061428/97‑1 (MQ=255)
                                        tAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCa                                                            <  2:2241549/100‑1 (MQ=255)
                                         aaaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                           >  2:1016486/1‑100 (MQ=255)
                                           aCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCTCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGc                                                         <  1:1328258/100‑1 (MQ=255)
                                                cATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTCCACTATTACTGAGAATACACCCATCAAGCATAAt                                                    >  1:2751691/1‑100 (MQ=255)
                                                  tACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCg                                                  >  2:644728/1‑100 (MQ=255)
                                                   aCCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGa                                                 >  2:2579184/1‑100 (MQ=255)
                                                      aTTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAgg                                              >  2:1534204/1‑100 (MQ=255)
                                                       tttGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATCCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAgg                                              <  1:2597241/99‑1 (MQ=255)
                                                         tGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTc                                           >  2:1821532/1‑100 (MQ=255)
                                                         tGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAAGACACCCATCAAGCATAATCGAAGGTTc                                           <  1:1387014/100‑1 (MQ=255)
                                                          gAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCa                                          >  2:1210766/1‑100 (MQ=255)
                                                            gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                        >  1:2260212/1‑100 (MQ=255)
                                                             cGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAata                                       <  2:575124/100‑1 (MQ=255)
                                                               aTCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAatat                                      >  1:499611/1‑99 (MQ=255)
                                                                tCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCg                                    <  2:1847787/100‑1 (MQ=255)
                                                                tCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCg                                    <  1:828589/100‑1 (MQ=255)
                                                                 cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                   >  1:2701225/1‑100 (MQ=255)
                                                                 cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCAGCAAGCATAATCGAAGGTTCAATATCg                                    <  2:1371499/99‑1 (MQ=255)
                                                                  gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                  >  1:923483/1‑100 (MQ=255)
                                                                  gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                  >  1:416775/1‑100 (MQ=255)
                                                                  gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                  >  2:2331138/1‑100 (MQ=255)
                                                                   cccGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGccc                                 >  2:2845061/1‑100 (MQ=255)
                                                                    ccGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                   >  1:1628899/1‑97 (MQ=255)
                                                                        tGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtttt                            >  2:295345/1‑100 (MQ=255)
                                                                         gTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                           >  1:844156/1‑100 (MQ=255)
                                                                          ttCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTg                          <  2:192894/100‑1 (MQ=255)
                                                                           tCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGa                         <  2:416777/100‑1 (MQ=255)
                                                                           tCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGa                         <  2:1158828/100‑1 (MQ=255)
                                                                            cccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                        >  2:1839513/1‑100 (MQ=255)
                                                                             ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                        <  1:1897475/99‑1 (MQ=255)
                                                                             ccccATACTGTAAAGCCGGAGACATGCTCCGCCTTTACAGTATTACTGAGAATACACCCATCAAGCATACTCGAAGGTTCAATATCGCCCCTTTTTGagc                       >  1:1076139/1‑98 (MQ=255)
                                                                              cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                      >  2:760535/1‑100 (MQ=255)
                                                                              cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                      <  2:1400889/100‑1 (MQ=255)
                                                                              cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                      >  2:439736/1‑100 (MQ=255)
                                                                                cATACTGTAAAGCCGGAGACATGCTCCGACTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGcc                    >  1:1887567/1‑100 (MQ=255)
                                                                                 aTACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGcc                    <  1:930286/99‑1 (MQ=255)
                                                                                  tACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGATCAATATCGCCCCTTTTTGATCGCCAg                  >  1:468265/1‑100 (MQ=255)
                                                                                   aCTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                 <  1:1487489/100‑1 (MQ=255)
                                                                                    cTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                >  1:2330951/1‑100 (MQ=255)
                                                                                    cTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATTGCCTGc                 >  2:1998221/1‑99 (MQ=37)
                                                                                    cTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                 >  1:2735277/1‑99 (MQ=38)
                                                                                      gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATAGCCCCTTTTTGATCGCCAGGGa               >  2:2652351/1‑99 (MQ=255)
                                                                                      gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATAGCCCCTTCTTGATCGCAAACGAt              >  1:527417/1‑100 (MQ=255)
                                                                                       tAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAAAATCGCAACTTTTTCATCCACACCGAt              >  2:1994946/1‑99 (MQ=21)
                                                                                        aaaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc            >  2:620269/1‑100 (MQ=255)
                                                                                        aaaGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc            >  2:934567/1‑100 (MQ=38)
                                                                                         aaGCCGGAGACATGTTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg           <  1:997888/100‑1 (MQ=255)
                                                                                         aaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg           >  1:1348299/1‑100 (MQ=255)
                                                                                         aaGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg           >  2:2687627/1‑100 (MQ=38)
                                                                                            ccGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCa        <  2:1190495/100‑1 (MQ=255)
                                                                                                 gaCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTg   >  1:506142/1‑100 (MQ=255)
                                                                                                  aCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGc  <  2:743438/100‑1 (MQ=255)
                                                                                                  |                                                                                                    
GGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGC  >  NC_000913/4520402‑4520599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: