Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 120,154 Δ1 bp 20.7% intergenic (+19/+24) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913120,1540T.20.7% 324.6 / 85.2 121intergenic (+19/+24)ampE/aroPampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
Reads supporting (aligned to +/- strand):  ref base T (50/46);  new base . (8/17);  total (58/63)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.48e-01

Only 100 of 124 total aligned reads displayed.

GGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGG  >  NC_000913/120057‑120253
                                                                                                 |                                                                                                    
    tCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACgg                                                                                               >  1:2833003/1‑100 (MQ=255)
     cAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACggg                                                                                              <  2:602993/100‑1 (MQ=255)
       aTGGCGAAGAAAACGTCGTTCGTGGTGGGCGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACggg                                                                                              <  1:1326149/98‑1 (MQ=255)
         ggCGAAGAAAACCTCGTTCTTGGTCGCGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGAGTAGAGATTATTGCCCTCACCCTGTACGGGTGAg                                                                                          >  1:247493/1‑100 (MQ=255)
         ggCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAg                                                                                          <  2:428199/100‑1 (MQ=255)
          gCGAAGAAAGTCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAgg                                                                                         <  1:1912011/100‑1 (MQ=255)
          gCGAAGAAAACCGCGTTCGTGGTCGTGGTGGTGAGTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAgg                                                                                         <  1:2609553/100‑1 (MQ=255)
           cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAgg                                                                                         >  1:1413969/1‑99 (MQ=255)
           cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                        <  2:1598019/100‑1 (MQ=255)
           cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                        <  2:2333664/100‑1 (MQ=255)
           cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                        <  2:219525/100‑1 (MQ=255)
           cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                        <  1:512780/100‑1 (MQ=255)
           cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                        <  1:1125336/100‑1 (MQ=255)
           cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGGTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAggg                                                                                        <  1:2359313/100‑1 (MQ=255)
            gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGTGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                       <  1:1615464/100‑1 (MQ=255)
            gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAggg                                                                                        <  1:2348667/99‑1 (MQ=255)
            gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                       <  2:2067152/100‑1 (MQ=255)
            gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                       <  2:2545422/100‑1 (MQ=255)
            gaagaaAACCTCGTTCGTGGTCGTGGTGGGGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                       <  1:2651457/100‑1 (MQ=255)
             aagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCg                                                                                      <  1:1180027/100‑1 (MQ=255)
              agaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGcaa                                                                                     <  2:1036025/100‑3 (MQ=39)
                aaaaCCTGGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGc                                                                                       <  1:2909673/96‑1 (MQ=255)
                aaaaCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTag                                                                                   <  2:66905/100‑1 (MQ=255)
                 aaaCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTaga                                                                                  >  2:1536742/1‑100 (MQ=255)
                   aCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagaga                                                                                >  1:2201780/1‑100 (MQ=255)
                   aCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagaga                                                                                >  2:1551549/1‑100 (MQ=255)
                    ccTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagagag                                                                               >  2:759082/1‑100 (MQ=255)
                     cTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAgagaga                                                                              >  1:2316131/1‑100 (MQ=255)
                        gttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTa                                                                           >  2:1854254/1‑100 (MQ=255)
                          tcgtggtcgtggTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAAt                                                                         >  1:2592886/1‑100 (MQ=255)
                          tcgtggtcgtggTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAAt                                                                         >  1:1998018/1‑100 (MQ=255)
                           cgtggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATg                                                                        <  1:2663331/100‑1 (MQ=255)
                           cgtggtcgtcgtGGTTATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATg                                                                        <  1:305029/100‑1 (MQ=255)
                            gtggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgc                                                                       >  2:1060090/1‑100 (MQ=255)
                            gtggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgc                                                                       <  1:407102/100‑1 (MQ=255)
                              ggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgcgc                                                                     <  2:2669941/100‑1 (MQ=255)
                              ggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgcgc                                                                     <  2:1753437/100‑1 (MQ=255)
                              ggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgcgc                                                                     >  1:471227/1‑100 (MQ=255)
                                 cgtggtGCTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTACAGCTCATTGCCCTCACCCTGTACGGGTGGGGGCACCGCGAGGTTAATGCGCttt                                                                  >  1:826081/1‑100 (MQ=255)
                                   tggtggttATTGAACTACTGACTATTTAAGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATTCGCTTTTa                                                                <  1:1096166/100‑1 (MQ=255)
                                   tggtggtgATTGCACTACTGACTATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTa                                                                <  1:699018/100‑1 (MQ=255)
                                             tGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGc                                                      >  1:749969/1‑100 (MQ=255)
                                             tGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGc                                                      <  1:2226960/100‑1 (MQ=255)
                                               cACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCgg                                                    <  1:1854245/100‑1 (MQ=255)
                                               cACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCgg                                                    >  2:359521/1‑100 (MQ=255)
                                                actactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGt                                                   >  1:2597759/1‑100 (MQ=255)
                                                  tactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGttt                                                 >  1:2860170/1‑100 (MQ=255)
                                                  tactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGttt                                                 <  1:2021668/100‑1 (MQ=255)
                                                    ctGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtc                                               >  1:2016165/1‑100 (MQ=255)
                                                     tGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtct                                              <  1:1716753/100‑1 (MQ=255)
                                                     tGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtct                                              >  2:1068359/1‑100 (MQ=255)
                                                      gACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtctc                                             >  1:191007/1‑100 (MQ=255)
                                                      gACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtcta                                             >  2:1716761/1‑99 (MQ=255)
                                                      gACGATTTACGGGGCGTGGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtctc                                             <  1:539448/100‑1 (MQ=255)
                                                       aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTctct                                            <  1:2424426/100‑1 (MQ=255)
                                                        cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTGCGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGattgc                                               >  2:2528851/1‑94 (MQ=255)
                                                         gATTTACGGGGCGTTGGGGTAAAGATTAGTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCttt                                          <  1:1491150/100‑1 (MQ=255)
                                                          aTTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTa                                         <  2:1243242/100‑1 (MQ=255)
                                                          aTTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTa                                         >  1:2237525/1‑100 (MQ=255)
                                                          aTTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTCTTACGGCTTTGGCGGTTTTCTCTTTa                                         >  2:1237819/1‑100 (MQ=255)
                                                           tttACGGGTCGTTGGTGTAAAGATTATTGGCCTCACGCTGGACGGGTGAGGGCGTAGAGAGATTAAGGCGCTTTTACGGCTTTGGCGGTTGTCTCTTTaa                                        <  1:1752194/100‑1 (MQ=255)
                                                             tACGGGGGGTTGGTGTAAAGACTATTCCCCTCACCCTGTACGGGTGAGAGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCCGTTTTCTCTTTAAAc                                      <  2:2251169/100‑1 (MQ=255)
                                                             tACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAAc                                      <  1:2084268/100‑1 (MQ=255)
                                                             tACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAAc                                      >  1:561595/1‑100 (MQ=255)
                                                              aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACa                                     <  2:2092027/100‑1 (MQ=255)
                                                              aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACa                                     >  2:2718060/1‑100 (MQ=255)
                                                              aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACa                                     <  2:856140/100‑1 (MQ=255)
                                                              aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACa                                     >  2:1407971/1‑100 (MQ=255)
                                                                   gCGTGGGTGTAAAGATTAGTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAg                                <  1:273300/100‑1 (MQ=255)
                                                                    cGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTGTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGc                               <  1:858608/100‑1 (MQ=255)
                                                                     gtgggTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGcc                              <  1:1907318/97‑1 (MQ=255)
                                                                     gTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGcc                              <  1:1280985/100‑1 (MQ=255)
                                                                      ttGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCg                             >  1:1078966/1‑100 (MQ=255)
                                                                      ttGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCg                             <  1:1151919/100‑1 (MQ=255)
                                                                        ggTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGAt                           >  1:367811/1‑100 (MQ=255)
                                                                        ggTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGAt                           <  1:1461044/100‑1 (MQ=255)
                                                                        ggTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGAt                           >  1:1220497/1‑100 (MQ=255)
                                                                          tgtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAc                         >  1:2282276/1‑100 (MQ=255)
                                                                           gtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAcc                        >  1:2822674/1‑100 (MQ=255)
                                                                           gtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAcc                        >  2:413264/1‑100 (MQ=255)
                                                                            tAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCt                       >  2:2004026/1‑100 (MQ=255)
                                                                             aaaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTa                      >  2:364892/1‑100 (MQ=255)
                                                                              tctctctacgCCCTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTaa                     >  2:2076337/10‑100 (MQ=37)
                                                                                gATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAaca                   >  2:276744/1‑100 (MQ=255)
                                                                                gATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTATTTAAACAGATAGCCGATGCCTAaca                   >  2:1656078/1‑100 (MQ=255)
                                                                                gATTATTGCCCTCACCCTGTACGGGTGAGCGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGCTTTTCTCTTTAAACAGATAGCCGATACCTAaca                   <  1:1755299/100‑1 (MQ=255)
                                                                                     ttGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATc              <  1:1078145/100‑1 (MQ=255)
                                                                                       gCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAg            <  2:1220502/100‑1 (MQ=255)
                                                                                       gCCCTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAg            >  2:2550722/1‑100 (MQ=255)
                                                                                        cccTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc           >  2:1080780/1‑100 (MQ=255)
                                                                                        cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc           >  2:269523/1‑100 (MQ=255)
                                                                                        cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc           >  2:226562/1‑100 (MQ=255)
                                                                                        cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc           >  2:2243702/1‑100 (MQ=255)
                                                                                        cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc           >  2:1890383/1‑100 (MQ=255)
                                                                                          cTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGcc          <  1:2538849/99‑1 (MQ=255)
                                                                                            cccccTGTACGGGTGAGGGCGTATAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACATATAGCCGATACCTAACACGATCAGcc          <  1:1143578/95‑1 (MQ=255)
                                                                                              cccTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCCTAcc     >  1:649907/1‑100 (MQ=255)
                                                                                                 tGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCggg  <  1:267577/100‑1 (MQ=255)
                                                                                                 tGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCggg  <  2:2026100/100‑1 (MQ=255)
                                                                                                 tGTACGGGAGAGGGCGTAGAGAGCTCAATGCGCTTTTTCGGCTTTGGCGGATTTCTAGTTCAACAGATAGGCGATACCAAACACGAGAGGCCATACCgg   >  1:2804092/1‑99 (MQ=255)
                                                                                                 |                                                                                                    
GGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGG  >  NC_000913/120057‑120253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: