Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,868,865:1 +G 15.6% intergenic (+23/‑90) yeaG → / → yeaH protein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,868,8651.G15.6% 359.7 / 70.2 109intergenic (+23/‑90)yeaG/yeaHprotein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base . (48/44);  new base G (4/13);  total (52/57)
Fisher's exact test for biased strand distribution p-value = 3.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.78e-01

Only 100 of 110 total aligned reads displayed.

GATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTG  >  NC_000913/1868767‑1868962
                                                                                                   |                                                                                                 
 ntgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTa                                                                                                 >  1:2512588/2‑100 (MQ=255)
 atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAAGTTa                                                                                                 >  1:911428/1‑100 (MQ=255)
   tggaaaaGGCTACACCCGTAAACAGGTGCGGTTACTGTGCGAATGGTATGTGCGCGTAAGTAAATCGTCTTAACAACCCTGGCCCGGTACACAGGTTAcc                                                                                               >  1:152254/4‑100 (MQ=255)
   ggAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTAcc                                                                                               <  2:2631081/100‑1 (MQ=255)
     aaaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCgg                                                                                             <  2:817317/100‑1 (MQ=255)
      aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCggg                                                                                            <  2:2143469/100‑1 (MQ=255)
      aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCggg                                                                                            <  1:1929291/100‑1 (MQ=255)
      aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCggg                                                                                            <  1:360490/100‑1 (MQ=255)
      aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCggg                                                                                            <  2:1493882/100‑1 (MQ=255)
      aaaaGGCGACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCggg                                                                                            <  2:1934922/100‑1 (MQ=255)
       aagggCTACGCCCGGAATCATCTTCTTTTACGGTGCGAAGGGTATGTGCTCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGc                                                                                           <  2:536195/97‑1 (MQ=255)
       aaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTGAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGc                                                                                           <  1:136978/100‑1 (MQ=255)
       aaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGc                                                                                           <  2:2124642/100‑1 (MQ=255)
       aaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGc                                                                                           <  2:1075232/100‑1 (MQ=255)
        aaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGcc                                                                                          <  2:622123/100‑1 (MQ=255)
        aaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGcc                                                                                          >  2:1015294/1‑100 (MQ=255)
        aaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGcc                                                                                          <  2:2749932/100‑1 (MQ=255)
         aGGCTACGCCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGcca                                                                                         <  2:2610969/100‑2 (MQ=255)
         aGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGcca                                                                                         <  2:2675887/100‑2 (MQ=39)
         aGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGcca                                                                                         <  2:753900/100‑2 (MQ=39)
          ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTa                                                                                        <  1:2152199/100‑1 (MQ=255)
           gCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccagn                                                                                       <  1:2351434/100‑4 (MQ=38)
            cTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTCACAACCCTGGCCCGGTACACATGTTACCGGGCCTACa                                                                                      >  2:2092900/1‑100 (MQ=255)
             tccaaccGTAAACAGGTGCGTTTCCTGGGCGAATGGTAGTTGCGCGAACGTAAATCGTCTTAATAACCCTGGCCCGGTAACATGTGTACCGGGccaggg                                                                                      <  1:1941088/94‑5 (MQ=21)
              acacCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAAc                                                                                    >  1:1900449/1‑100 (MQ=255)
              acacCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAAc                                                                                    <  1:411576/100‑1 (MQ=255)
               cacCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCtgggttn                                                                                   <  2:2512603/100‑7 (MQ=255)
                 cccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGa                                                                                  >  2:2220268/1‑99 (MQ=255)
                  ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACa                                                                                >  1:984652/1‑100 (MQ=255)
                  ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccagggttgtta                                                                                <  1:2307917/100‑11 (MQ=25)
                   cGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAg                                                                               <  2:1878561/100‑1 (MQ=255)
                    gTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGc                                                                              <  2:2188566/100‑1 (MQ=255)
                      aaaCAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGa                                                                            <  2:271924/100‑1 (MQ=255)
                        aCAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAAc                                                                          <  1:2777795/100‑1 (MQ=255)
                           ggTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGt                                                                       >  2:580277/1‑100 (MQ=255)
                             tGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTgg                                                                     <  2:78354/100‑1 (MQ=255)
                              gCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTggg                                                                    >  2:2786498/1‑100 (MQ=255)
                               cGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGc                                                                   >  2:2226884/1‑100 (MQ=255)
                                  ttACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTg                                                                <  2:2836442/100‑1 (MQ=255)
                                   tACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTga                                                               >  2:2507137/1‑100 (MQ=255)
                                    aCTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTgag                                                              >  2:2394936/1‑100 (MQ=255)
                                    aCTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAACGAACCGTGGGCCTgag                                                              >  1:957189/1‑100 (MQ=255)
                                     cTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTgaga                                                             <  2:144442/100‑1 (MQ=255)
                                     cTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTgaga                                                             >  1:887364/1‑100 (MQ=255)
                                       gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAg                                                           >  2:2674437/1‑100 (MQ=255)
                                           gAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGc                                                       >  2:2134187/1‑100 (MQ=255)
                                           gAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGc                                                       <  1:2394921/100‑1 (MQ=255)
                                           gAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGc                                                       <  1:580274/100‑1 (MQ=255)
                                               ggTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCAGCAaca                                                   >  2:1005317/1‑100 (MQ=255)
                                                ntATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAacac                                                  >  1:2539247/2‑100 (MQ=255)
                                                gTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAacac                                                  >  2:412683/1‑100 (MQ=255)
                                                 tATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAAcaca                                                 <  2:2139741/100‑1 (MQ=255)
                                                    ttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAgg                                               <  1:377736/99‑1 (MQ=255)
                                                     tGCGCGTACGTAAATCGTCTTAAAAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCg                                             >  2:2471103/1‑100 (MQ=255)
                                                       cgcgTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTa                                           >  2:658464/1‑100 (MQ=255)
                                                       cgcgTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTa                                           <  1:533228/100‑1 (MQ=255)
                                                         cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGCCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGc                                         <  1:2263318/100‑1 (MQ=255)
                                                          ttacgtaAATGGTCATAACAACCCTGGCCCGGTACAAATGTTATCGGGCTTACAACGACAGCCAACCGTGGGCGTGAGAAGCGGCAACACAGGCGt                                            >  1:1183959/2‑96 (MQ=255)
                                                          gtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCa                                        <  1:1086201/100‑1 (MQ=255)
                                                           tacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCAt                                       >  2:34348/1‑100 (MQ=255)
                                                             cgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATAc                                     >  1:1446978/1‑100 (MQ=255)
                                                             cgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATAc                                     <  1:2436513/100‑1 (MQ=255)
                                                                    cGTCTTAACGGCCCTGGCCCGGTAAACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATCCAGTTGGc                              <  1:551100/100‑1 (MQ=255)
                                                                     gTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                              >  2:1735604/1‑99 (MQ=255)
                                                                     gTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCa                             >  1:1047376/1‑100 (MQ=255)
                                                                     gTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGACCTGAGCAGCGGCAACACAGGCGTAGCATACAGTTGGCa                             >  1:2049135/1‑100 (MQ=255)
                                                                      tCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCaa                            >  2:465913/1‑100 (MQ=255)
                                                                       cTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCaaa                           <  2:342613/100‑1 (MQ=255)
                                                                         tAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATg                         >  2:708447/1‑100 (MQ=255)
                                                                         tAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGAGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTTGCAAAt                          >  1:968707/1‑99 (MQ=255)
                                                                         tAACAACCCTGGCCCGGTACACATGTTACCGGGCCTAAAACCACAGCGAAACGTGGGCCTGAGAAGCGGCAACACCGGCGTAGCATACAGTTGGCaaa                           >  1:559284/1‑98 (MQ=255)
                                                                          aacaacCCTGGCCCGGTACACATGTTAGCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTTGCaaa                           <  1:1933346/97‑1 (MQ=255)
                                                                          aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACACCGACAGCGAACCGTGGGCCTGAGAAGCGGCAACCCAGGCGTAGCATACAGTTGGCAAATg                         >  1:1831305/1‑99 (MQ=255)
                                                                          aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgt                        <  1:1343077/100‑1 (MQ=255)
                                                                          aacaacCCTGGCCCGGGCCACAGGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATg                         <  1:2718515/99‑1 (MQ=255)
                                                                           acaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgta                       <  1:1404401/100‑1 (MQ=255)
                                                                            caacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACAAAGGCGTAGCATACAGTTGGCAAATgtag                      >  1:1942485/1‑100 (MQ=255)
                                                                                 cTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgg                 >  1:2504363/1‑100 (MQ=255)
                                                                                 cTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgg                 >  2:1829682/1‑100 (MQ=255)
                                                                                  tGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg                >  1:2318971/1‑100 (MQ=255)
                                                                                  tGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg                >  2:643582/1‑100 (MQ=255)
                                                                                  tGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg                >  1:1103176/1‑100 (MQ=255)
                                                                                   ggCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggg               <  2:2049146/100‑1 (MQ=255)
                                                                                   ggCCCGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggg               >  2:166425/1‑100 (MQ=255)
                                                                                    gCCCGGTACACATGTTACCGGGCCTACAACGCCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg              <  2:818555/100‑1 (MQ=255)
                                                                                    gCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg              >  2:656812/1‑100 (MQ=255)
                                                                                    gCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg              >  1:1091322/1‑100 (MQ=255)
                                                                                    gCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGCGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggg               <  2:1474966/99‑1 (MQ=255)
                                                                                    gCCCGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAACTGTAGTACggggg              >  1:497089/1‑100 (MQ=255)
                                                                                     cccGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggggg             >  2:2513258/1‑100 (MQ=255)
                                                                                      ccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGc            <  2:77836/100‑1 (MQ=255)
                                                                                       cGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCa           <  2:1777063/100‑1 (MQ=255)
                                                                                           acacATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATg       <  2:1390840/100‑1 (MQ=255)
                                                                                           acacATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATg       <  1:1355449/100‑1 (MQ=255)
                                                                                            cacaTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGa      >  2:70198/1‑100 (MQ=255)
                                                                                            cacaTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGa      >  2:1174629/1‑100 (MQ=255)
                                                                                            cacaTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGa      >  1:1362445/1‑100 (MQ=255)
                                                                                             acaTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGAc     <  2:1111338/100‑1 (MQ=255)
                                                                                               aTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCt   <  2:2071955/100‑1 (MQ=255)
                                                                                                tGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTg  <  1:1064353/100‑1 (MQ=255)
                                                                                                   |                                                                                                 
GATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTG  >  NC_000913/1868767‑1868962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: