Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,950,966:1 +C 15.8% coding (135/567 nt) yecD → isochorismatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,950,9651.C15.8% 397.0 / 78.7 120coding (134/567 nt)yecDisochorismatase family protein
Reads supporting (aligned to +/- strand):  ref base . (48/53);  new base C (9/10);  total (57/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.83e-01

Only 100 of 127 total aligned reads displayed.

GGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGT  >  NC_000913/1950867‑1951064
                                                                                                   |                                                                                                   
ggtgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcgc                                                                                                    <  1:2542324/100‑1 (MQ=255)
ggtgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcgc                                                                                                    <  1:2680209/100‑1 (MQ=255)
 gtgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcgcg                                                                                                   <  2:830779/100‑1 (MQ=255)
  tgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATAGCGCCGGGAAGCTGGCGGAGAAATTTcccg                                                                                                   >  1:1831731/1‑99 (MQ=255)
   gATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGcc                                                                                                 >  2:230546/1‑100 (MQ=255)
   gATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCAGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGcc                                                                                                 >  1:2811781/1‑100 (MQ=255)
     tCGATTTACAAGAAGGCATCTTACCTTTTGGCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTcgccaccag                                                                                              <  1:659618/100‑7 (MQ=255)
     tCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAATCTGGCGCGAAATTTCGCCGCCAg                                                                                               <  2:2262453/100‑1 (MQ=255)
      cGATTTACAAGAAGGCATCTTGCCTTTTGCCGGTGGTCCACATACGGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAg                                                                                               <  1:2334000/99‑1 (MQ=255)
      cGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAGc                                                                                              <  2:2202324/100‑1 (MQ=255)
      cGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAGc                                                                                              <  2:1923038/100‑1 (MQ=255)
       gagttACAAGAAGGCGTCTTAGCTTTTGCCGGTGGTCCACATACGGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAGc                                                                                              <  1:1884472/96‑1 (MQ=37)
       gATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAAACGCCCCGGGAAGCTGGCGCGAAATTTCGCCGCCAGc                                                                                              <  2:1283063/99‑1 (MQ=38)
        aTTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCgg                                                                                            <  2:2547760/100‑1 (MQ=255)
        aTTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCgg                                                                                            <  1:77240/100‑1 (MQ=255)
           tACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCCGGCGGCGAAATTTCGCGCCAGCGGTCa                                                                                         >  1:490888/1‑100 (MQ=255)
            aCAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAg                                                                                        <  2:1840321/100‑1 (MQ=255)
            aCAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAg                                                                                        <  2:1193859/100‑1 (MQ=255)
             cAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGc                                                                                       >  1:1596033/1‑100 (MQ=255)
             cAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGACCGCGAAATTTCGCGCCAGCGCTCa                                                                                         >  1:314220/1‑98 (MQ=255)
              aagaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGcc                                                                                      <  2:617957/100‑1 (MQ=255)
               agaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGcc                                                                                      >  1:145674/1‑99 (MQ=255)
                 aagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGGTGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCgt                                                                                   >  2:2896354/1‑100 (MQ=255)
                    gCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGtt                                                                                <  2:2875098/100‑1 (MQ=255)
                      aTCTTGCCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTc                                                                              <  1:951024/100‑1 (MQ=255)
                      aTCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGtt                                                                                <  1:2271340/98‑1 (MQ=255)
                      aTCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTc                                                                              >  2:1882122/1‑100 (MQ=255)
                       tCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCt                                                                             <  2:2590196/100‑1 (MQ=255)
                         ttACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCTGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTgg                                                                           >  1:1370329/1‑100 (MQ=255)
                         ttACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTgg                                                                           <  2:2274960/100‑1 (MQ=255)
                          tACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGt                                                                          >  1:2368966/1‑100 (MQ=255)
                            ccTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTgc                                                                        <  2:495634/100‑1 (MQ=255)
                              ttttGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTgcgc                                                                      >  1:908792/1‑100 (MQ=255)
                                 tGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGtt                                                                   <  2:629419/100‑1 (MQ=255)
                                  gCCGGTGGTCCACATACTGCCGATGAGGTAGTTACTCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTg                                                                  >  1:557675/1‑100 (MQ=255)
                                   ccGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTgg                                                                 <  1:293730/100‑1 (MQ=255)
                                       tggtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTgg                                                             >  2:2367042/1‑100 (MQ=255)
                                        ggtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTgn                                                             <  1:2539355/99‑2 (MQ=255)
                                         ggccACATACTGCCGAGGAGGTAGTTAACCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTc                                                           <  2:342902/98‑1 (MQ=255)
                                          tCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCt                                                          <  2:961272/100‑1 (MQ=255)
                                          tCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCt                                                          >  2:2387978/1‑100 (MQ=255)
                                                  cTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTa                                                  <  2:540563/100‑1 (MQ=255)
                                                  cTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTa                                                  >  1:1322803/1‑100 (MQ=255)
                                                    gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACg                                                >  1:2892614/1‑100 (MQ=255)
                                                    gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACg                                                >  1:2364101/1‑100 (MQ=255)
                                                    gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTGTCTGGTGCGCGTTGGCTGGTCTGCCGATTACg                                                >  1:250815/1‑100 (MQ=255)
                                                    gCCGATGAGGTAGTTAATCGCGCCGGCAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACg                                                >  2:1776416/1‑100 (MQ=255)
                                                     ccGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGc                                               <  2:469183/100‑1 (MQ=255)
                                                     ccGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGc                                               <  2:37188/100‑1 (MQ=255)
                                                      cGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGc                                               >  1:202382/1‑99 (MQ=255)
                                                      cGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCt                                              <  2:2230461/100‑1 (MQ=255)
                                                          gAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCTTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGagg                                          <  2:1971091/100‑3 (MQ=255)
                                                          gAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAg                                          <  1:1712877/100‑1 (MQ=255)
                                                          gAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAg                                          <  2:59771/100‑1 (MQ=255)
                                                          gAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAg                                          <  1:416487/100‑1 (MQ=255)
                                                            ggTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCa                                        <  2:1067374/100‑1 (MQ=255)
                                                             gTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCAt                                       >  1:2245993/1‑100 (MQ=255)
                                                              tAGTTAATCGCGCCGGGATGCTGGCGGTGAATGTTTGTGCTAGCGGTCAGCCCGTGTTTCTGATTCGCGTGGGCTGGTCTGCCGATTATGCTGAAGCAtt                                      <  1:1122092/100‑1 (MQ=255)
                                                              tAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCAtt                                      >  1:2545082/1‑100 (MQ=255)
                                                              tAGTTAATCGCGACGGGAAGCTGGCCGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGTCTGGTCTGCCGATTACGCTGAAGCAtt                                      >  2:142868/1‑100 (MQ=255)
                                                               aGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTa                                     >  1:2702104/1‑100 (MQ=255)
                                                               aGTTAATAGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTa                                     >  2:1532671/1‑100 (MQ=255)
                                                                gTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaa                                    <  1:10622/100‑1 (MQ=255)
                                                                gTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaa                                    >  2:640719/1‑100 (MQ=255)
                                                                 ttAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaaa                                   >  2:135755/1‑100 (MQ=255)
                                                                 ttAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaaa                                   <  2:2212254/100‑1 (MQ=255)
                                                                 ttAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaaa                                   <  1:2168342/100‑1 (MQ=255)
                                                                  tAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTTGTGCGCTTTGGCTGGTCTGCCGATTACGCTAAAGCATTaaaa                                  >  1:1555615/1‑100 (MQ=255)
                                                                  tAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaaaa                                  <  2:2513288/100‑1 (MQ=255)
                                                                   aaTCGCTCCGGGAATCTGGCGGCTAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaaa                                   <  2:1994995/98‑1 (MQ=255)
                                                                   aaTCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAAc                                 >  1:1744330/1‑100 (MQ=255)
                                                                    aTCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGTCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACa                                <  1:657099/100‑1 (MQ=255)
                                                                      cgcgTCGGGAAGCTGGCGGCGAAATTTGGCGCCAGCGGTCGGCCCGTGTTTCTGGTGCGCTTTGGCTGGTTTGCCGATTTCGCTGAAGCATTAAAACAGc                              >  1:1154632/1‑100 (MQ=255)
                                                                      cgcgCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGc                              >  2:2204545/1‑100 (MQ=255)
                                                                       gcgcCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGcc                             <  1:896404/100‑1 (MQ=255)
                                                                        cgcCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCg                            <  2:1327102/100‑1 (MQ=255)
                                                                           ctgaccgCTGGCGCGAAATTTCGCCGCCAGAGGGCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTc                         >  1:1912898/7‑100 (MQ=37)
                                                                           cGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTc                         <  1:484241/100‑1 (MQ=255)
                                                                           cGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTc                         <  1:2355161/100‑1 (MQ=255)
                                                                            gggAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCg                        <  2:1311798/100‑1 (MQ=255)
                                                                             gaccgCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGa                       >  2:651148/5‑100 (MQ=38)
                                                                               ccgCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATg                     >  2:2287581/3‑100 (MQ=39)
                                                                               ccgCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATg                     >  2:2584300/3‑100 (MQ=39)
                                                                               ccgCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATg                     >  2:2799747/3‑100 (MQ=39)
                                                                               ccgCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCACGTGTTTCTGGTGCGCGTTGGCTGCTCTGCCGATTACGCTGAAGAATTAAAACAGCCGGTCGATg                     >  1:276824/3‑100 (MQ=37)
                                                                               aaGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATg                     <  2:2045277/100‑1 (MQ=255)
                                                                                aGCTGGCGGCGGAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTTGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATgc                    <  1:784937/100‑1 (MQ=255)
                                                                                 gCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATgcg                   >  2:2854242/1‑100 (MQ=255)
                                                                                  cTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATgcgc                  <  1:1606966/100‑1 (MQ=255)
                                                                                  cTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATgcgc                  >  1:2153976/1‑100 (MQ=255)
                                                                                   tGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGcc                 <  1:2029826/100‑1 (MQ=255)
                                                                                    ggcggcGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGccc                <  1:1093601/100‑1 (MQ=255)
                                                                                    ggcggcGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGccc                >  2:1152175/1‑100 (MQ=255)
                                                                                        gcgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCAc            >  2:1173354/1‑100 (MQ=255)
                                                                                         ggAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGATGGTCTGCCGGGTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCAcc           >  2:2605828/2‑100 (MQ=255)
                                                                                         cgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCAcc           <  2:2364116/100‑1 (MQ=255)
                                                                                           aaaTTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCgg         <  2:1410554/100‑1 (MQ=255)
                                                                                              tttCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTa      <  2:1514875/100‑1 (MQ=255)
                                                                                               ttCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTaa     >  2:832495/1‑100 (MQ=255)
                                                                                                 cgcgCCAGCGGTCAGCCCGTTTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAg   <  1:2061018/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGT  >  NC_000913/1950867‑1951064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: