Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 380,022:1 (G)10→11 100% intergenic (‑141/+47) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0221.G66.1% 265.1 / 153.7 115intergenic (‑141/+47)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base . (21/18);  new base G (41/35);  total (62/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 119 total aligned reads displayed.

ATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGG  >  NC_000913/379930‑380121
                                                                                               |                                                                                                   
tatatgtcaatgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATc                                                                                               <  1:869700‑M1/14‑1 (MQ=255)
 atatgtcaatgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCg                                                                                              <  1:2806196‑M1/15‑1 (MQ=255)
    tgtcaatgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCa                                                                                           <  2:2638627‑M1/18‑1 (MQ=255)
    cAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAgg                                                                                         >  1:378747/1‑100 (MQ=255)
      tcaatgtatacccccctatagtatataacactggagaataaaatttatcctgtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAgg                                                                                         <  1:1178899‑M1/20‑1 (MQ=255)
      tcaatgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAgg                                                                                         <  2:823478‑M1/20‑1 (MQ=255)
      tcaatgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAgg                                                                                         >  1:383240‑M1/81‑100 (MQ=255)
      tcaatgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAgg                                                                                         <  1:896253‑M1/20‑1 (MQ=255)
       caatgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAggg                                                                                        <  2:233518‑M1/21‑1 (MQ=255)
        aatgcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAggg                                                                                        >  1:688188‑M1/79‑99 (MQ=255)
           tacccccctatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGgc                                                                                   >  1:491867‑M2/19‑100 (MQ=255)
           gcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAgg                                                                                    >  2:1044802‑M1/76‑100 (MQ=255)
           gcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAgg                                                                                    >  1:362378‑M1/76‑100 (MQ=255)
           gcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAgg                                                                                    >  1:1772344‑M1/76‑100 (MQ=255)
           gcatacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAg                                                                                     >  2:189282‑M1/76‑99 (MQ=255)
            catacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGgc                                                                                   <  1:761483‑M1/26‑1 (MQ=255)
             cccccctatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGgccgc                                                                                >  2:815697‑M2/17‑100 (MQ=255)
             atacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGgcc                                                                                  <  2:2705015‑M1/27‑1 (MQ=255)
             atacccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGgcc                                                                                  <  1:23939‑M1/27‑1 (MQ=255)
              ccccctatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGgccgcc                                                                               <  2:736491‑M2/85‑1 (MQ=255)
                cctccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGgccgc                                                                                <  1:2270539‑M1/29‑1 (MQ=255)
                cccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGgccgcc                                                                               <  2:2912165‑M1/30‑1 (MQ=255)
                cccccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGgccgcc                                                                               <  1:2360067‑M1/30‑1 (MQ=255)
                  cccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc                                                                             >  2:959602‑M1/69‑100 (MQ=255)
                  cccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc                                                                             <  1:232894‑M1/32‑1 (MQ=255)
                   ccctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                                           >  1:1650494‑M1/68‑100 (MQ=255)
                    cctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                                           >  1:1692733‑M1/67‑100 (MQ=255)
                    cctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                                           >  2:1699823‑M1/67‑100 (MQ=255)
                    ataGTATACAACACTGGAGAATAAAAATTATCCGGAGAATGTGGTCGGGAAACAAAGCGGAAA‑‑GGGGGGGGCTAATCGGCAGCGAAGGCCGCCCCGGa                                                                          <  1:2532070/98‑1 (MQ=255)
                     ctatagtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagagtaaaGGGGGGGCTAATCGGCAGGGCAGGCCGCCCCGGAt                                                                         <  1:1682940‑M1/35‑1 (MQ=255)
                        tatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGc                                                                      <  2:491870‑M2/95‑1 (MQ=255)
                         agtatataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc                                                                      <  2:150359‑M1/39‑1 (MQ=255)
                         agtatataacactggagaataaaatttatccggtgaaggtggtcggaaaacaaagagtaaaGGGGGGGGCTAATCGGCAGGGAAGGTCGCCCCGGatacc                                                                      >  2:1974108‑M1/62‑98 (MQ=255)
                            taACACTGGAGAATAAAACTTATCCGGTCAATGTGGTCGGGAAACAAAGAGCAAA‑‑AGGAGGGGCTAATCGGCAGGGAAGGACGCCCCGGATAgcgggcgg                                                                >  1:713155/1‑100 (MQ=255)
                            tAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGc                                                                  >  2:22704‑M2/2‑100 (MQ=255)
                            atataacactggagaataaaatttatccggtgaatgtggtcgggaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                                   >  1:1870579‑M1/59‑100 (MQ=255)
                            atataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggc                                                                  <  1:5665‑M1/42‑1 (MQ=255)
                              ataacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcg                                                                 >  1:2496882‑M1/57‑100 (MQ=255)
                              acacTCGAATATACAATTTATCCGGTGAACGTCGTCGGAAAACAAAGCGGAAA‑‑GGCGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg                                                                    <  1:2585605/94‑1 (MQ=255)
                               taacactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg                                                                <  1:2132467‑M1/45‑1 (MQ=255)
                                 acactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCa                                                              >  2:896256‑M1/54‑100 (MQ=255)
                                 acactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCa                                                              >  1:20457‑M1/54‑100 (MQ=255)
                                  cactggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAg                                                             >  2:2503071‑M1/53‑100 (MQ=255)
                                    ctggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa                                                           <  2:2426853‑M1/50‑1 (MQ=255)
                                    ctggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa                                                           <  2:2747572‑M1/50‑1 (MQ=255)
                                    ctggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa                                                           <  1:2188856‑M1/50‑1 (MQ=255)
                                    ctggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa                                                           <  1:2242529‑M1/50‑1 (MQ=255)
                                     tggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                                          >  2:1015633‑M1/50‑100 (MQ=255)
                                      ggagaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                                         <  1:2247153‑M1/52‑1 (MQ=255)
                                         gaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                                      >  1:2128755‑M1/46‑100 (MQ=255)
                                         gaataaaatttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc                                                     <  2:1547152‑M1/55‑1 (MQ=255)
                                             aaaatttatccggtgaatatggtcggaagacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa                                                  <  1:820533‑M1/59‑1 (MQ=255)
                                                 tttatccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAttt                                              <  2:728414‑M1/63‑1 (MQ=255)
                                                    atccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                                           >  1:1566759‑M1/35‑100 (MQ=255)
                                                    atccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                                           >  2:1855479‑M1/35‑100 (MQ=255)
                                                    atccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                                          >  2:842438‑M1/35‑100 (MQ=255)
                                                     tccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                                          >  2:761486‑M1/34‑100 (MQ=255)
                                                      ccggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                         <  1:1909093‑M1/68‑1 (MQ=255)
                                                       cggtgaatgtggtcggaaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                       >  1:1469653‑M1/32‑100 (MQ=255)
                                                         gtgagtgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGCAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCcan                                          >  1:2621036‑M1/30‑95 (MQ=255)
                                                          tgaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAg                                     >  2:949126‑M1/29‑100 (MQ=255)
                                                           gaatgtggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa                                    <  1:1946788‑M1/73‑1 (MQ=255)
                                                             atgttgtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACg                                  >  1:2598095‑M1/26‑100 (MQ=255)
                                                              tgtggtcggaaaacaaagaggaaaGGTGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACgg                                 <  2:1241345‑M1/76‑1 (MQ=255)
                                                                tggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGc                               >  1:1116873‑M1/23‑100 (MQ=255)
                                                                tggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGc                               >  1:2260135‑M1/23‑100 (MQ=255)
                                                                 ggtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                              >  2:913429‑M1/22‑100 (MQ=255)
                                                                  gtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTg                             >  1:2191467‑M1/21‑100 (MQ=255)
                                                                  gtcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTg                             <  2:2889658‑M1/80‑1 (MQ=255)
                                                                   tcggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                            <  2:2847237‑M1/81‑1 (MQ=255)
                                                                    cggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                            >  1:333380‑M1/19‑99 (MQ=255)
                                                                    cggaaaacaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa                           <  1:2281554‑M1/82‑1 (MQ=255)
                                                                       aaaacaaagaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGtt                       <  2:116585‑M1/85‑1 (MQ=255)
                                                                       aCAAAGAGGAA‑‑GGGGGGGGGCTGATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGTAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc                     >  1:132117/1‑100 (MQ=255)
                                                                          acaaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc                     <  2:308255‑M1/88‑1 (MQ=255)
                                                                           caaagaggaaaGGTGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                    >  1:1241340‑M1/12‑100 (MQ=255)
                                                                           caaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                    <  1:129728‑M1/89‑1 (MQ=255)
                                                                           caaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                    >  2:2762057‑M1/12‑100 (MQ=255)
                                                                           caaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                    >  1:1680434‑M1/12‑100 (MQ=255)
                                                                            aaagaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg                   <  2:1317617‑M1/90‑1 (MQ=255)
                                                                              agaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc                 >  2:820716‑M1/9‑100 (MQ=255)
                                                                               gaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGTCTGCAACTTgcagccc                >  2:2242678‑M1/8‑97 (MQ=255)
                                                                               gaggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAcc                >  2:2286403‑M1/8‑100 (MQ=255)
                                                                               gaggaaaGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGTCTGCAACTTGCAGCCCg               >  2:931655‑M1/8‑100 (MQ=255)
                                                                                ggaaaggggggggCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGt              >  1:636911/6‑100 (MQ=255)
                                                                                ggaGGGGGGGGGGGTAAGCGGAAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAGGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGt              <  1:1336893/98‑1 (MQ=255)
                                                                                aggaaaGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGTCTGCAACTTGCAGCCCg               >  2:2806214‑M1/7‑100 (MQ=255)
                                                                                 ggaaaGGCGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATGTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGt              >  1:1565009‑M1/6‑100 (MQ=255)
                                                                                 ggaaaGGGGCGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGCCAGAAGGAATCAGACTTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc                 <  1:710279‑M1/92‑1 (MQ=255)
                                                                                 gaaaggggggggCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGTCTGCAACTTGCAGCCCGtc             <  2:499395/96‑2 (MQ=255)
                                                                                 gaaagGGGGGGGCTAAGCGGCAGGGAAGGCAGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGTCTGCAACTTgcagccc                >  1:1142691‑M1/6‑94 (MQ=255)
                                                                                    aggggggggCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGTCTGCAACTTGCAGCCCGtctga          <  2:2725065/99‑5 (MQ=38)
                                                                                       GGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGAAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatc       <  2:1109000‑M1/100‑1 (MQ=255)
                                                                                         GGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatca      <  2:1169109‑M1/100‑1 (MQ=255)
                                                                                            GGGCTAATCGGCAGGGAAGGCCGCCCTGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcatc    <  2:2738834‑M1/100‑1 (MQ=255)
                                                                                            GGGCTAATCGGCAGGGAAGGCCGCCCCGGCTAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGAAAGTTGCAGACCGTTAACatcatc    <  1:676771‑M1/100‑1 (MQ=255)
                                                                                            GGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcatc    >  2:1989288‑M1/1‑100 (MQ=255)
                                                                                            GGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcatc    >  1:1021489‑M1/1‑100 (MQ=255)
                                                                                             GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg   <  1:587479‑M1/100‑1 (MQ=255)
                                                                                              GCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCgg  <  2:101600‑M1/100‑1 (MQ=255)
                                                                                               |                                                                                                   
ATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGG  >  NC_000913/379930‑380121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: