| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 781,818:1 | (A)6→7 | 100% | intergenic (+166/‑267) | lysQ → / → nadA | tRNA‑Lys/quinolinate synthase, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 781,812 | 1 | . | A | 88.5% | 177.1 / 17.8 | 61 | intergenic (+160/‑273) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
| Reads supporting (aligned to +/- strand): ref base . (2/5); new base A (24/30); total (26/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGT‑AAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTC > NC_000913/781750‑781910 | gCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAg < 2:646479/100‑1 (MQ=16)gCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAg > 2:216146/1‑100 (MQ=16) ccGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAgg < 2:2674948/100‑1 (MQ=255) gCTGGCGGCGACGGCCCAACAGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAgg < 1:921220/98‑1 (MQ=11) tCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATc > 2:763644/1‑100 (MQ=255) cgcgGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATcc > 1:1137575/1‑100 (MQ=255) cgcgGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCATAAAGGCGCTTTTTTGCTATTCAGGCATcc > 1:2523847/1‑100 (MQ=255) cggcgACGGCCCAAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTc < 1:186761/100‑1 (MQ=255) gctaCGGCCCGAAGGGCGAGCGAAGCGAGTCCTCCTTCACGACCCACCAATGT‑AAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCaa > 1:1232415/4‑99 (MQ=255) gACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAAtt < 2:145713/100‑1 (MQ=255) caCGGCCCGAAGGGCGAGCGCAGCGAGTCATCCTGCACGCCCCACCAATGTAAAAAAAGCGCCCTAAAAGCGCTTTTTTGCTATTCAGGCATcc > 1:441242/2‑94 (MQ=14) cGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTc < 2:2883175/100‑1 (MQ=255) gCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCAc > 1:1342336/1‑100 (MQ=255) gCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCAc < 2:2603510/100‑1 (MQ=255) gCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGT‑AAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACt < 2:950582/100‑1 (MQ=255) cccAAAGGGCGAGCGCAGCGAGTAATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCAc < 2:120237/99‑1 (MQ=17) ccGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACtt > 2:596731/1‑100 (MQ=255) ccGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGT‑AAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACttt < 2:299092/100‑1 (MQ=255) cGAAGGGCGAGCGAAGCGAGTCTTCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACttt < 2:2517148/100‑1 (MQ=255) gAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTg > 2:2574994/1‑100 (MQ=255) aaGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGt < 1:39786/100‑1 (MQ=255) ggCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTaaa > 1:466589/1‑100 (MQ=255) cgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAcc > 2:2362006/1‑100 (MQ=255) cgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAcc < 1:191501/100‑1 (MQ=255) cgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAcc < 1:2303083/100‑1 (MQ=255) gatcgaagctaGTAATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAcc < 1:731904/96‑1 (MQ=21) gagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAcc < 1:309737/99‑1 (MQ=255) gagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCt > 2:436816/1‑100 (MQ=255) agcggagcgaGTCATCCTGCACGTCCCACCCATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACGTTGTAAAcc < 1:570551/98‑1 (MQ=18) agcgaagcgaGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtg > 2:1900913/1‑100 (MQ=255) gcgaagcgaGTCATCCTGCACGACCCAACAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTaaa < 1:491692/95‑1 (MQ=255) cgcagcgaCTCATCCTGCACGACCCACCAATGT‑AAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAcc < 1:2253243/92‑1 (MQ=255) cgaagcgaGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtgat > 2:1017853/1‑100 (MQ=255) cgaagcgaGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtgat < 1:1468156/100‑1 (MQ=255) cgaagcgaGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtga < 2:2753044/99‑1 (MQ=255) gaagcgaGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtgatg > 1:1741958/1‑100 (MQ=255) gaagcgaGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtgatg < 2:1465408/100‑1 (MQ=255) aagcgaGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtgatga < 1:2302667/100‑1 (MQ=255) gcgaGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACa > 1:2464898/1‑100 (MQ=255) aGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATc < 2:1651778/99‑1 (MQ=255) aGTCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCg < 2:1344231/100‑1 (MQ=255) tCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTc < 1:1043815/100‑1 (MQ=255) tCATCCTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTc > 1:808277/1‑100 (MQ=255) cTGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGc < 2:2655752/100‑1 (MQ=255) tGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCt > 1:2434859/1‑100 (MQ=255) tGCACGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCt > 1:2872570/1‑100 (MQ=255) cACGGCCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTa < 1:2753091/100‑1 (MQ=255) aCGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTAc < 2:2279099/100‑1 (MQ=255) cGACCCACCAATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACt > 1:599869/1‑100 (MQ=255) caccaATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgca > 2:463907/1‑100 (MQ=255) caccaATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgca > 2:1056770/1‑100 (MQ=255) caccaATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgca < 2:2106671/100‑1 (MQ=255) caccaATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgca > 2:1040790/1‑100 (MQ=255) ccaATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaag > 1:514966/1‑100 (MQ=255) ccaATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaag < 2:2218629/100‑1 (MQ=255) ccaATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaag < 2:1087214/100‑1 (MQ=255) ccaATGT‑AAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaagc < 2:637490/100‑1 (MQ=255) caATGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTTCTGTgcaagc > 2:1692920/1‑100 (MQ=255) aattGT‑AAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaagca < 1:2144804/97‑1 (MQ=255) aaTGTAAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaagca < 1:1086205/100‑1 (MQ=255) gT‑AAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAActc > 1:1448820/1‑100 (MQ=255) | GCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGT‑AAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTC > NC_000913/781750‑781910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |