Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 120,158:1 +A 20.8% intergenic (+23/+20) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913120,1581.A20.8% 304.0 / 107.4 120intergenic (+23/+20)ampE/aroPampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
Reads supporting (aligned to +/- strand):  ref base . (50/45);  new base A (8/17);  total (58/62)
Fisher's exact test for biased strand distribution p-value = 7.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.40e-02

Only 100 of 128 total aligned reads displayed.

TCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCA  >  NC_000913/120061‑120257
                                                                                                  |                                                                                                   
tCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACgg                                                                                                   >  1:2833003/1‑100 (MQ=255)
 cagtGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACGGACGATTTACGGGGCGGTGGTGTAAAGATTATTGCCCGCACCCTGTACggg                                                                                                  <  1:1912091/97‑1 (MQ=255)
 cAATGGCCAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACggg                                                                                                  >  2:1528067/1‑100 (MQ=255)
   aTGGCGAAGAAAACGTCGTTCGTGGTGGGCGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACggg                                                                                                  <  1:1326149/98‑1 (MQ=255)
     ggCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAg                                                                                              <  2:428199/100‑1 (MQ=255)
      gCGAAGAAAGTCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAgg                                                                                             <  1:1912011/100‑1 (MQ=255)
      gCGAAGAAAACCGCGTTCGTGGTCGTGGTGGTGAGTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAgg                                                                                             <  1:2609553/100‑1 (MQ=255)
       cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAggg                                                                                            <  2:2833022/100‑1 (MQ=255)
       cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAgg                                                                                             >  1:1413969/1‑99 (MQ=255)
       cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                            <  2:2333664/100‑1 (MQ=255)
       cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                            <  1:512780/100‑1 (MQ=255)
       cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                            <  2:219525/100‑1 (MQ=255)
       cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                            <  2:1598019/100‑1 (MQ=255)
       cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                            <  1:1125336/100‑1 (MQ=255)
       cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGGTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAggg                                                                                            <  1:2359313/100‑1 (MQ=255)
        gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGTGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                           <  1:1615464/100‑1 (MQ=255)
        gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAggg                                                                                            <  1:2348667/99‑1 (MQ=255)
        gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                           <  1:2775878/100‑1 (MQ=255)
        gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                           <  1:445967/100‑1 (MQ=255)
        gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                           <  2:2067152/100‑1 (MQ=255)
        gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                           <  2:2545422/100‑1 (MQ=255)
        gaagaaAACCTCGTTCGTGGTCGTGGTGGGGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                           <  1:2651457/100‑1 (MQ=255)
         aagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCg                                                                                          <  1:1180027/100‑1 (MQ=255)
            aaaaCCTGGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGc                                                                                           <  1:2909673/96‑1 (MQ=255)
            aaaaCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTag                                                                                       <  2:66905/100‑1 (MQ=255)
             aaaCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTaga                                                                                      >  2:1536742/1‑100 (MQ=255)
               aCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagaga                                                                                    >  2:1551549/1‑100 (MQ=255)
               aCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagaga                                                                                    >  1:2201780/1‑100 (MQ=255)
               aCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATGTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagaga                                                                                    >  1:2506381/1‑100 (MQ=255)
                ccTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagagag                                                                                   >  2:759082/1‑100 (MQ=255)
                    gttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTa                                                                               >  2:1854254/1‑100 (MQ=255)
                      tcgtggtcgtggTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAAt                                                                             >  1:2592886/1‑100 (MQ=255)
                       cgtggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATg                                                                            <  1:2663331/100‑1 (MQ=255)
                        gtggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgc                                                                           <  1:407102/100‑1 (MQ=255)
                        gtggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgc                                                                           >  2:1060090/1‑100 (MQ=255)
                          ggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgcgc                                                                         <  2:2669941/100‑1 (MQ=255)
                             cgtggtGCTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTACAGCTCATTGCCCTCACCCTGTACGGGTGGGGGCACCGCGAGGTTAATGCGCttt                                                                      >  1:826081/1‑100 (MQ=255)
                               tggtggttATTGAACTACTGACTATTTAAGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATTCGCTTTTa                                                                    <  1:1096166/100‑1 (MQ=255)
                                     tgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCtt                                                              >  1:1149033/1‑100 (MQ=255)
                                      gATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTACCCTCACCTTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCttt                                                             >  1:93852/1‑100 (MQ=255)
                                         tGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGc                                                          >  1:749969/1‑100 (MQ=255)
                                         tGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGc                                                          <  1:2226960/100‑1 (MQ=255)
                                           cACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCgg                                                        <  1:1854245/100‑1 (MQ=255)
                                              tactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGttt                                                     <  1:2021668/100‑1 (MQ=255)
                                              tactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGttt                                                     >  1:2860170/1‑100 (MQ=255)
                                               actGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGtttt                                                    >  2:2585822/1‑100 (MQ=255)
                                                ctGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtc                                                   >  1:2016165/1‑100 (MQ=255)
                                                ctGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtc                                                   <  2:1901865/100‑1 (MQ=255)
                                                 tGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtct                                                  >  2:1068359/1‑100 (MQ=255)
                                                 tGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtct                                                  <  1:1716753/100‑1 (MQ=255)
                                                  gACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtctc                                                 >  1:191007/1‑100 (MQ=255)
                                                  gACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtcta                                                 >  2:1716761/1‑99 (MQ=255)
                                                  gACGATTTACGGGGCGTGGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtctc                                                 <  1:539448/100‑1 (MQ=255)
                                                   aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTctct                                                <  1:2424426/100‑1 (MQ=255)
                                                    cGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTGCGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGattgc                                                   >  2:2528851/1‑94 (MQ=255)
                                                     gATTTACGGGGCGTTGGGGTAAAGATTAGTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCttt                                              <  1:1491150/100‑1 (MQ=255)
                                                      aTTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTa                                             >  1:2237525/1‑100 (MQ=255)
                                                      aTTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTa                                             <  2:1243242/100‑1 (MQ=255)
                                                      aTTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTCTTACGGCTTTGGCGGTTTTCTCTTTa                                             >  2:1237819/1‑100 (MQ=255)
                                                         tACGGGGGGTTGGTGTAAAGACTATTCCCCTCACCCTGTACGGGTGAGAGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCCGTTTTCTCTTTAAAc                                          <  2:2251169/100‑1 (MQ=255)
                                                         tACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAAc                                          >  1:561595/1‑100 (MQ=255)
                                                         tACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAAc                                          <  1:2084268/100‑1 (MQ=255)
                                                          aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACa                                         <  2:2092027/100‑1 (MQ=255)
                                                          aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACa                                         <  2:856140/100‑1 (MQ=255)
                                                          aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACa                                         >  2:2718060/1‑100 (MQ=255)
                                                           cGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAg                                        >  1:1510876/1‑100 (MQ=255)
                                                             gggCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGAt                                      >  2:476867/1‑100 (MQ=255)
                                                               gCGTGGGTGTAAAGATTAGTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAg                                    <  1:273300/100‑1 (MQ=255)
                                                                cGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTGTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGc                                   <  1:858608/100‑1 (MQ=255)
                                                                  ttGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCg                                 >  1:1078966/1‑100 (MQ=255)
                                                                  ttGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCg                                 <  1:1151919/100‑1 (MQ=255)
                                                                    ggTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGAt                               <  2:16573/100‑1 (MQ=255)
                                                                    ggTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGAt                               >  1:367811/1‑100 (MQ=255)
                                                                    ggTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGAt                               >  1:1220497/1‑100 (MQ=255)
                                                                      tgtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAc                             >  1:2282276/1‑100 (MQ=255)
                                                                       gtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAcc                            >  2:413264/1‑100 (MQ=255)
                                                                         aaaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTa                          >  2:364892/1‑100 (MQ=255)
                                                                          tctctctacgCCCTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTaa                         >  2:2076337/10‑100 (MQ=37)
                                                                            gATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTATTTAAACAGATAGCCGATGCCTAaca                       >  2:1656078/1‑100 (MQ=255)
                                                                            gATTATTGCCCTCACCCTGTACGGGTGAGCGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGCTTTTCTCTTTAAACAGATAGCCGATACCTAaca                       <  1:1755299/100‑1 (MQ=255)
                                                                                attGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTCGGAGGTTGTCTATTTAGACAGATAGCCGATACCTTacac                      >  1:2614850/1‑97 (MQ=255)
                                                                                 ttGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATc                  <  1:1078145/100‑1 (MQ=255)
                                                                                   gCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAg                <  2:1220502/100‑1 (MQ=255)
                                                                                    cccTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc               >  2:1080780/1‑100 (MQ=255)
                                                                                    cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc               >  2:226562/1‑100 (MQ=255)
                                                                                    cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc               >  2:269523/1‑100 (MQ=255)
                                                                                    cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc               >  2:2243702/1‑100 (MQ=255)
                                                                                    cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc               >  1:2325397/1‑100 (MQ=255)
                                                                                    cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc               >  2:1890383/1‑100 (MQ=255)
                                                                                     cgtCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc               <  1:1311239/97‑1 (MQ=255)
                                                                                     ccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGcc              >  1:2872011/1‑100 (MQ=255)
                                                                                      cTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGcc              <  1:2538849/99‑1 (MQ=255)
                                                                                          cccTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCCTAcc         >  1:649907/1‑100 (MQ=255)
                                                                                            cTGTACGGGTGAGGGCGTAGGGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTATCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCgg       >  1:1779116/1‑100 (MQ=255)
                                                                                             tGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCggg      <  2:2026100/100‑1 (MQ=255)
                                                                                             tGTACGGGAGAGGGCGTAGAGAGCTCAATGCGCTTTTTCGGCTTTGGCGGATTTCTAGTTCAACAGATAGGCGATACCAAACACGAGAGGCCATACCgg       >  1:2804092/1‑99 (MQ=255)
                                                                                              gTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGa     >  1:1923535/1‑100 (MQ=255)
                                                                                                aCGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATc   <  2:1237/100‑1 (MQ=255)
                                                                                                 cGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCa  <  2:2858443/100‑1 (MQ=255)
                                                                                                 cGGGTGAGGGCGTAGAGAGATTAACGCGCTTTTACGGCTGTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATc   <  2:2895458/99‑1 (MQ=255)
                                                                                                  |                                                                                                   
TCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCA  >  NC_000913/120061‑120257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: