Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,850,719:1 +T 16.7% intergenic (+26/‑141) sppA → / → ansA protease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,850,7191.T16.7% 302.6 / 70.6 102intergenic (+26/‑141)sppA/ansAprotease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1
Reads supporting (aligned to +/- strand):  ref base . (50/35);  new base T (6/11);  total (56/46)
Fisher's exact test for biased strand distribution p-value = 1.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01

Only 100 of 102 total aligned reads displayed.

GCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTC  >  NC_000913/1850627‑1850816
                                                                                             |                                                                                                 
gCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGcc                                                                                            <  2:1843417/100‑1 (MQ=255)
 cTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCca                                                                                           <  2:2086789/100‑1 (MQ=255)
 cTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCca                                                                                           <  2:1884123/100‑1 (MQ=255)
  tGGCCGCGTTTAATGACCCACAAAACCGTTATGTGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcac                                                                                          <  1:1097038/100‑1 (MQ=255)
  tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCcac                                                                                          <  2:2775758/100‑1 (MQ=255)
  tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCcac                                                                                          <  2:1516262/100‑1 (MQ=255)
  tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGacac                                                                                          <  1:2230481/100‑1 (MQ=255)
  tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcac                                                                                          <  2:2410111/100‑1 (MQ=255)
  tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcac                                                                                          <  2:1222372/100‑1 (MQ=255)
  tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcac                                                                                          <  1:217873/100‑1 (MQ=255)
  tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCGTGTACTGAGTGGCCGACAGATCGTCGGCcac                                                                                          <  1:2722936/100‑1 (MQ=255)
    gCCGCGTTTAATGACCCCCAAGGCCGGTATGCGTTTTGCCTGAACTTCGCCAACATGCGTTAAGTCTTGTACTGGGTGGCCGACGATCTGTCGGcc                                                                                            <  1:590881/96‑1 (MQ=255)
    gCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACat                                                                                        <  2:2255697/100‑1 (MQ=255)
    gCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACat                                                                                        >  2:2831857/1‑100 (MQ=255)
    gCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTGGCCTGACCTGAGCCAATATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGGcactc                                                                                        <  1:1616025/100‑3 (MQ=255)
    gCCGCGTTTAATGACCCACAAAACCGTTAGGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcactc                                                                                        <  1:329221/100‑3 (MQ=255)
      cgcgTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACatta                                                                                      >  2:1205863/1‑100 (MQ=255)
        cgTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACattatt                                                                                    <  2:2415477/100‑1 (MQ=255)
        cgTTTAATGACCCACAAAACCGATATGCGTTTTGACTGACATGCGCCAACATGCGTTAAATCGTGTACTCAGTGGCCGACAGATCGTCGGGCACatta                                                                                      >  1:1370237/1‑98 (MQ=255)
          tttcacgccccACAAAACCGTTATGCGTTTCGCCTGACCTACGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACATATCGTCGGCCACATTAtttt                                                                                  <  1:1095405/92‑1 (MQ=255)
            tAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTAtttttt                                                                                <  1:624428/100‑1 (MQ=255)
             aaTGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTa                                                                               <  2:2916232/100‑1 (MQ=255)
             aaTGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTa                                                                               <  2:2242628/100‑1 (MQ=255)
              acgACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTAc                                                                              <  1:221301/98‑1 (MQ=255)
              aTGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAATGGCCGACAGATCGTCGGCCACATTATTTTTTAc                                                                              >  1:1535774/1‑100 (MQ=255)
                  cccACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcg                                                                          >  1:1165154/1‑100 (MQ=255)
                  cccACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcg                                                                          >  1:2707572/1‑100 (MQ=255)
                  cccACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcg                                                                          >  1:2633635/1‑100 (MQ=255)
                    cacaAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcgac                                                                        >  2:1323264/1‑100 (MQ=255)
                    cacaAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcgac                                                                        <  2:2152774/100‑1 (MQ=255)
                    cacaAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcgac                                                                        <  2:2222780/100‑1 (MQ=255)
                    cacaAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcgac                                                                        >  2:2691182/1‑100 (MQ=255)
                    cacaAAACCGTTATGCGTTGTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcgac                                                                        <  1:891143/100‑1 (MQ=255)
                     acaAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcgacg                                                                       <  1:2569297/100‑1 (MQ=255)
                       aaaaCCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGaa                                                                     >  2:1903480/1‑100 (MQ=255)
                        aaaCCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAAt                                                                    <  1:1121233/100‑1 (MQ=255)
                           ccGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCct                                                                 >  2:380368/1‑100 (MQ=255)
                             nttATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCctct                                                               >  1:1942224/2‑100 (MQ=255)
                             gTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCctct                                                               <  2:207938/100‑1 (MQ=255)
                             gTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCctct                                                               <  1:1501066/100‑1 (MQ=255)
                               tATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTc                                                             <  1:2715888/100‑1 (MQ=255)
                                 tGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTccc                                                           >  2:2049218/1‑100 (MQ=255)
                                   cGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCCGCCACATTATTTTTTACGTCGACGAATCCTCTTccc                                                           <  1:1203380/98‑1 (MQ=255)
                                    gTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCt                                                        >  1:896097/1‑100 (MQ=255)
                                        tGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGttt                                                    >  1:261335/1‑100 (MQ=255)
                                         gCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTc                                                   >  2:199296/1‑100 (MQ=255)
                                         gCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTc                                                   >  1:134674/1‑100 (MQ=255)
                                            tGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGcc                                                <  1:1579423/100‑1 (MQ=255)
                                             gACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGccc                                               <  2:252175/100‑1 (MQ=255)
                                               ccTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCa                                             >  2:1347774/1‑100 (MQ=255)
                                               ccTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCa                                             >  2:2891732/1‑100 (MQ=255)
                                                cTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCa                                             <  1:1324951/99‑1 (MQ=255)
                                                 tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCata                                           >  2:1009772/1‑100 (MQ=255)
                                                  gcgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCatat                                          >  2:2324583/1‑100 (MQ=255)
                                                  gcgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCatat                                          >  2:1671906/1‑100 (MQ=255)
                                                    gtcAACATGCGTTCAGTCTTGTACTGAGTGGCCGACAGATCGACGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATAttt                                        >  2:1974170/3‑100 (MQ=255)
                                                    gcCAACATGCGGTAAGTCTTGCACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCGTCTTCCCGCTGTTTCGCTCCATAtt                                         >  1:2178048/1‑99 (MQ=255)
                                                     ccAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTc                                       >  1:573885/1‑100 (MQ=255)
                                                     ccAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTc                                       <  2:1141155/100‑1 (MQ=255)
                                                       aaCATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCt                                     >  2:465096/1‑100 (MQ=255)
                                                       aaCATGCGTGCAGTCTTGTACTGCGTGGTCGACAGATGGTCGGCCACATTATTTTTTACTTCGTCGAATCCTCTTCTAGCTGTTTCGCTCCGTATTTc                                       >  1:1208592/1‑98 (MQ=255)
                                                         cATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTa                                   <  2:1872168/100‑1 (MQ=255)
                                                           tGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCTGCCACATTATTTTTGACGTCGACGAATCCTCTTCCCGCTGGTTCGCCCCATATTTCCTTATc                                 <  1:2712085/100‑1 (MQ=255)
                                                            gCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATcc                                >  1:1504284/1‑100 (MQ=255)
                                                              gTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGc                              >  2:575996/1‑100 (MQ=255)
                                                              gTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGc                              >  2:603368/1‑100 (MQ=255)
                                                                tAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCAt                            <  1:1395540/100‑1 (MQ=255)
                                                                 aaGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATa                           >  2:1302901/1‑100 (MQ=255)
                                                                   gTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGt                         >  1:2182717/1‑100 (MQ=255)
                                                                   gTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCGCTTCCCGCTGTTTCGCCCCATATTTCATTATCCGCATAGt                         >  1:699767/1‑100 (MQ=255)
                                                                     cTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCAATATTTCCTTATCCGCATAGTAt                       >  2:1616568/1‑100 (MQ=255)
                                                                      ttGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATc                      >  2:2693063/1‑100 (MQ=255)
                                                                       tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATcn                     <  2:861106/100‑2 (MQ=255)
                                                                       tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCa                     <  2:502534/100‑1 (MQ=255)
                                                                       tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCa                     <  2:573888/100‑1 (MQ=255)
                                                                        gTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAg                    >  2:2388427/1‑100 (MQ=255)
                                                                         tACTGAGTGGCCGACAGCTCGTCGGCCACATTTTTTTTTACGTCTACGAATGCTCTTCCCGCTGCGTCCCTCCATATTTCCTTATCCGCATCGTATCAgg                   >  1:405312/1‑100 (MQ=255)
                                                                         aataatgTGGCCGACGATCTGTTGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAgg                   >  1:2537014/7‑100 (MQ=255)
                                                                          ataatgTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAgg                   >  2:109860/6‑99 (MQ=38)
                                                                          aCTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGt                  <  1:2497549/100‑1 (MQ=255)
                                                                            aatgTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTgc                >  2:1490694/4‑100 (MQ=39)
                                                                             gAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTgcg               >  2:2325233/1‑100 (MQ=255)
                                                                             atgTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTgcg               >  2:828166/3‑100 (MQ=255)
                                                                              aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTgcgc              <  2:2340237/100‑1 (MQ=255)
                                                                               gTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCt             >  2:640594/1‑100 (MQ=255)
                                                                               gTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATACTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCt             >  1:1184674/1‑100 (MQ=255)
                                                                               gTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCt             >  2:37076/1‑100 (MQ=255)
                                                                                tGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTtcgc              >  1:336207/1‑98 (MQ=255)
                                                                                tGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTc            >  2:1762058/1‑100 (MQ=255)
                                                                                 ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcc           >  1:2852787/1‑100 (MQ=255)
                                                                                 ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcc           >  2:2400071/1‑100 (MQ=255)
                                                                                   ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccc         >  2:94282/1‑100 (MQ=255)
                                                                                   ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccc         >  2:2137076/1‑100 (MQ=255)
                                                                                    cGACAGATCGTCGGCCACATTATTTTTTACGTCGACTAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATGCGCATAGTATCAGGTGCGCTccccc        >  1:523492/1‑100 (MQ=255)
                                                                                        aGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTTCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGcc    <  1:2049207/100‑1 (MQ=255)
                                                                                        aGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGcc    <  2:86999/100‑1 (MQ=255)
                                                                                        aGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGcc    >  1:1996575/1‑100 (MQ=255)
                                                                                         gATTGTCGGCCACATTATTTCTTGCGACGAAGCATCCTCTTCCTGCTGCGTCGCCGCATCTTCCGTTAGCCGCGTAGTATCGGGGGCGCTCCGCCTGcc    <  1:2007368/99‑1 (MQ=255)
                                                                                         gATCGTCGGCCACATTAGTTTTTACGGGGACGAATCCTCTTCCCGCTGTCTCGCCCCACATTTCCGTATCCCCATAGTATGAGGTGGGCCCGCCCTccc    >  1:970270/1‑99 (MQ=255)
                                                                                          aTCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCcctccctc  >  1:1453501/1‑100 (MQ=255)
                                                                                             |                                                                                                 
GCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTC  >  NC_000913/1850627‑1850816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: