New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4631855108 (1.040)7 (0.070) 5/166 NT 6.3% coding (1123/1938 nt) slt lytic murein transglycosylase, soluble
?NC_000913 = 4631875 108 (1.120)coding (1143/1938 nt) slt lytic murein transglycosylase, soluble
Rejected: Frequency below cutoff threshold.

TGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4631751‑4631855
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgcaacCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTAAC  <  NC_000913/4631875‑4631778
                                                                                                                                                                                                           
TGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCAT                                                                                                         >  2:2242236/1‑100
TGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCAT                                                                                                         <  2:1163886/100‑1
 GGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATG                                                                                                        <  2:1715414/100‑1
   AAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCA                                                                                                      <  2:2807999/100‑1
   AAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCA                                                                                                      >  2:2525483/1‑100
      CGAAAGAGAAAGAGGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGGCGTGAAGCTGAAGCAAAAGAGATTGTGCATCAACTCATGCAAC                                                                                                    >  2:860465/1‑99
                                 GGCAGGCGGATTTATTGATGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCATG                                                                        <  1:545494/100‑1
                                  GCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCATGA                                                                       <  1:783270/100‑1
                                  GCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCATGA                                                                       <  2:2185874/100‑1
                                   CAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCGAAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCATGAG                                                                      <  1:622223/100‑1
                                    AGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCATGAGT                                                                     <  1:1387202/100‑1
                                    AGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCATGAGT                                                                     <  1:1346936/100‑1
                                              ATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAA                                                           <  2:1147010/100‑1
                                                                                                     CAACCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTA    >  2:1137016/1‑100
                                                                                                       ACCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTAAC  >  2:2248470/1‑100
                                                                                                       ACCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTAAC  >  1:2308541/1‑100
                                                                                                       ACCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTAAC  >  2:2886802/1‑100
                                                                                                                                                                                                           
TGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4631751‑4631855
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgcaacCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTAAC  <  NC_000913/4631875‑4631778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.