Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258675 258683 9–776 101 [0] [0] 103 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)84 (0.890) 57/162 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

Only 100 of 101 total aligned reads displayed.

ATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCG  >  NC_000913/257810‑258006
                                                                                                 |                                                                                                   
aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAag                                                                                                   <  1:507076‑M1/100‑3 (MQ=255)
aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAag                                                                                                   >  1:2140909‑M1/1‑98 (MQ=255)
aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAag                                                                                                   >  1:2014503‑M1/1‑98 (MQ=255)
  cacaACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtg                                                                                                 >  1:845611‑M1/1‑96 (MQ=255)
  cacaACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtg                                                                                                 <  1:2859586‑M1/100‑5 (MQ=255)
  cacaACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtg                                                                                                 <  2:673496‑M1/100‑5 (MQ=255)
   acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgc                                                                                                <  1:1216555‑M1/100‑6 (MQ=255)
      acaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaa                                                                                             >  1:1202440‑M1/1‑92 (MQ=255)
       caGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaag                                                                                            <  2:16542‑M1/100‑10 (MQ=255)
        aGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaaga                                                                                           <  1:1198676‑M1/100‑11 (MQ=255)
          gagaTAGCAATGCCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAACATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaacat                                                                                          >  1:104750‑M1/1‑88 (MQ=255)
            gaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat                                                                                       >  2:501992‑M1/1‑86 (MQ=255)
             aTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatc                                                                                      <  1:2639836‑M1/100‑16 (MQ=255)
             aTAGCAAGGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatc                                                                                      <  2:2151663‑M1/100‑16 (MQ=255)
              tAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcg                                                                                     >  2:1752494‑M1/1‑84 (MQ=255)
              tAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcg                                                                                     <  2:2559176‑M1/100‑17 (MQ=255)
              tAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcg                                                                                     >  2:1753592‑M1/1‑84 (MQ=255)
                gCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgat                                                                                   >  1:2674116‑M1/1‑82 (MQ=255)
                gCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgat                                                                                   >  1:2633496‑M1/1‑82 (MQ=255)
                  aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattc                                                                                 >  2:2094489‑M1/1‑80 (MQ=255)
                   aTGACGTTACCGAGTGGACACCCGAAGAGAAGAAAAAAAAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattct                                                                                <  1:301451‑M1/100‑22 (MQ=255)
                        gTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc                                                                           >  1:11105‑M1/1‑74 (MQ=255)
                        gTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc                                                                           >  1:2684839‑M1/1‑74 (MQ=255)
                        gTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc                                                                           >  1:434837‑M1/1‑74 (MQ=255)
                         ttACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcg                                                                          <  2:246652‑M1/100‑28 (MQ=255)
                           aacgaGTGAACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGACCAGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcga                                                                         <  2:2509263‑M1/97‑29 (MQ=255)
                            ccGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgatt                                                                       >  2:1475478‑M1/1‑70 (MQ=255)
                             cGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattg                                                                      >  2:1848997‑M1/1‑69 (MQ=255)
                             cGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattg                                                                      <  2:1027104‑M1/100‑32 (MQ=255)
                             cGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattg                                                                      <  2:1027111‑M1/100‑32 (MQ=255)
                              gAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgt                                                                     >  2:2176104‑M1/1‑68 (MQ=255)
                               aGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtc                                                                    <  2:2351203‑M1/100‑34 (MQ=255)
                                 tGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctg                                                                  <  2:1182546‑M1/100‑36 (MQ=255)
                                   gACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggc                                                                >  2:2194050‑M1/1‑63 (MQ=255)
                                     cacCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctg                                                              <  2:1188885‑M1/100‑40 (MQ=255)
                                     cacCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctg                                                              >  2:231843‑M1/1‑61 (MQ=255)
                                       cccGAAGAGCAGATTGATCAAAAAATTTTCCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgta                                                            <  1:379051‑M1/100‑42 (MQ=255)
                                       cccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgta                                                            >  1:1268602‑M1/1‑59 (MQ=255)
                                       cccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgta                                                            <  2:1972730‑M1/100‑42 (MQ=255)
                                       cccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgta                                                            >  1:1899669‑M1/1‑59 (MQ=255)
                                       cccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgta                                                            >  2:564615‑M1/1‑59 (MQ=255)
                                         cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgta                                                            <  2:483790‑M1/98‑42 (MQ=255)
                                          gAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgc                                                         >  1:325051‑M1/1‑56 (MQ=255)
                                          gAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgc                                                         >  1:1048414‑M1/1‑56 (MQ=255)
                                              agCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtc                                                      <  1:913073‑M1/99‑48 (MQ=255)
                                               gCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaa                                                    >  1:195810‑M1/1‑51 (MQ=255)
                                               gCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaa                                                    >  2:1038271‑M1/1‑51 (MQ=255)
                                                cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                   >  2:2346944‑M1/1‑50 (MQ=255)
                                                 aGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacg                                                  <  2:2684855‑M1/100‑52 (MQ=255)
                                                  gATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgt                                                 >  2:864729‑M1/1‑48 (MQ=255)
                                                  gATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgt                                                 >  1:1635570‑M1/1‑48 (MQ=255)
                                                   aTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtt                                                <  2:2434437‑M1/100‑54 (MQ=255)
                                                     tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaa                                              >  2:1624201‑M1/1‑45 (MQ=255)
                                                     tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaa                                              <  1:2809846‑M1/100‑56 (MQ=255)
                                                     tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaa                                              >  1:2665123‑M1/1‑45 (MQ=255)
                                                     tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaa                                              >  2:142503‑M1/1‑45 (MQ=255)
                                                     tGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtacgcgtcaacgtgaa                                              <  1:1799794‑M1/100‑56 (MQ=255)
                                                      gATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaa                                             >  2:501970‑M1/1‑44 (MQ=255)
                                                       aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaac                                            <  1:85219‑M1/100‑58 (MQ=255)
                                                        tCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacc                                           >  1:2189102‑M1/1‑42 (MQ=255)
                                                        tCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacc                                           >  2:1787839‑M1/1‑42 (MQ=255)
                                                        tCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacc                                           <  1:2636825‑M1/100‑59 (MQ=255)
                                                         cAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccg                                          >  1:383251‑M1/1‑41 (MQ=255)
                                                          aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccgg                                         >  1:1626955‑M1/1‑40 (MQ=255)
                                                          aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccgg                                         >  1:2547865‑M1/1‑40 (MQ=255)
                                                          aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccgg                                         <  1:641482‑M1/100‑61 (MQ=255)
                                                           aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggc                                        <  2:2547880‑M1/100‑62 (MQ=255)
                                                           aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggc                                        <  2:1795374‑M1/100‑62 (MQ=255)
                                                            aaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggca                                       >  1:2384875‑M1/1‑38 (MQ=255)
                                                              aaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcacc                                     <  1:1383697‑M1/100‑65 (MQ=255)
                                                                 ttACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccgga                                  <  2:502339‑M1/100‑68 (MQ=255)
                                                                 ttACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccgga                                  <  1:564612‑M1/100‑68 (MQ=255)
                                                                  tACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaa                                 >  1:1253890‑M1/1‑32 (MQ=255)
                                                                  tACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaa                                 <  2:2097036‑M1/100‑69 (MQ=255)
                                                                   acagcacTAGGCCCGTATATTCGTTAATGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaag                                <  1:948323‑M1/97‑70 (MQ=255)
                                                                   aCCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaag                                <  1:1765802‑M1/100‑70 (MQ=255)
                                                                    ccGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagctaatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccgg                                   >  1:894195‑M1/1‑30 (MQ=255)
                                                                    ccGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaacagaaat                               >  2:1104806‑M1/1‑30 (MQ=255)
                                                                     cGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtg                              <  2:1542488‑M1/100‑72 (MQ=255)
                                                                         cTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtg                          <  2:1227277‑M1/100‑76 (MQ=255)
                                                                         cTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtg                          <  1:55496‑M1/100‑76 (MQ=255)
                                                                         cTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtg                          >  2:764234‑M1/1‑25 (MQ=255)
                                                                          tAGGCCCGTATATTCGTGAAGGTAagtgcaaagatcatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtg                          <  1:1702775‑M1/99‑76 (MQ=255)
                                                                              cccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattc                     >  2:2669131‑M1/1‑20 (MQ=255)
                                                                              cccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattc                     <  2:451997‑M1/100‑81 (MQ=255)
                                                                                 gTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                  <  1:462507‑M1/100‑84 (MQ=255)
                                                                                 gTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                  <  1:262569‑M1/100‑84 (MQ=255)
                                                                                 gTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgacaacgtgaaaccggcaccggaagtgcgtgaattctgg                  <  1:1558236‑M1/100‑84 (MQ=255)
                                                                                   atatTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgggg                <  2:1983723‑M1/100‑86 (MQ=255)
                                                                                    tatTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggc               <  2:792131‑M1/100‑87 (MQ=255)
                                                                                     atTCGTGAAGGTAagtgcaaagataatcgattctttttcgagtgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggct              >  1:1593572‑M1/1‑13 (MQ=255)
                                                                                       tCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgg            <  2:1936730‑M1/100‑90 (MQ=255)
                                                                                         gTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtaggcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg          <  2:86533‑M1/100‑92 (MQ=255)
                                                                                          tGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgg         >  2:167366‑M1/1‑8 (MQ=255)
                                                                                            aaGGTAagtgcaaagataatcgattctttttcgagtgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgcggccggtggat       <  1:1527001‑M1/100‑95 (MQ=255)
                                                                                            aaGGTAactgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggat       <  2:1837949‑M1/100‑95 (MQ=255)
                                                                                              ggTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatgg     <  1:142502‑M1/100‑97 (MQ=255)
                                                                                              ggTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatgg     <  1:1499863‑M1/100‑97 (MQ=255)
                                                                                                 aagtgcaaagataatcgattcttttttttttgttttgctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagc  >  1:821938‑M1/1‑1 (MQ=255)
                                                                                                 aagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagc  <  1:1894647‑M1/100‑100 (MQ=255)
                                                                                                 |                                                                                                   
ATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCG  >  NC_000913/257810‑258006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: