Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 14,116 A→G 15.6% intergenic (+37/‑52) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091314,1160AG15.6% 303.6 / 45.2 136intergenic (+37/‑52)dnaK/dnaJchaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
Reads supporting (aligned to +/- strand):  ref base A (57/56);  new base G (13/8);  total (72/64)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.19e-01

Only 100 of 139 total aligned reads displayed.

ACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCG  >  NC_000913/14018‑14215
                                                                                                  |                                                                                                   
acaacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTAACACGAGCGaa                                                                                                    >  1:113030/1‑100 (MQ=255)
  aacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAgg                                                                                                  >  2:2436389/1‑100 (MQ=255)
   acGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAggg                                                                                                 <  2:1079350/100‑1 (MQ=255)
   acGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAggg                                                                                                 <  1:1286280/100‑1 (MQ=255)
    cgcgAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAgggg                                                                                                >  1:2541813/1‑100 (MQ=255)
    cgcgAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAgggg                                                                                                >  2:2189582/1‑100 (MQ=255)
     gttaaaGAAGACGATGTTGTTGACGTCTAATTTCAAAAAAAAAAAAAAAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAgggg                                                                                                <  1:2000253/96‑1 (MQ=255)
     gcgAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGa                                                                                               >  1:2242667/1‑100 (MQ=255)
     gcgAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGa                                                                                               >  2:928641/1‑100 (MQ=255)
        aaaGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAAtt                                                                                            <  2:804118/100‑1 (MQ=255)
        aaaGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAAtn                                                                                            <  2:2260283/100‑2 (MQ=255)
         aaGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAAttt                                                                                           >  2:2581726/1‑100 (MQ=255)
                    gttgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATAGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGccc                                                                                <  1:1609093/100‑1 (MQ=37)
                     ttgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCg                                                                               <  2:307651/100‑1 (MQ=37)
                     ttgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCg                                                                               <  2:1412306/100‑1 (MQ=255)
                       gtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGTg                                                                             <  1:2715215/100‑1 (MQ=37)
                       gtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTg                                                                             >  2:2815372/1‑100 (MQ=255)
                       gtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTg                                                                             <  1:1414013/100‑1 (MQ=255)
                        tCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGc                                                                            <  2:2067961/100‑1 (MQ=255)
                         cGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGTgtc                                                                           <  2:745848/100‑3 (MQ=25)
                         cGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTAGACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGTg                                                                             <  1:1205933/98‑1 (MQ=25)
                          gACGCTGAATTTGAAGAAGTCACAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCACCCCGCCCGTGCAt                                                                          >  1:90936/1‑100 (MQ=255)
                           aCGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGTgtcag                                                                         <  1:2808241/100‑5 (MQ=25)
                            cGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTc                                                                        >  2:2654787/1‑100 (MQ=255)
                            cGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTc                                                                        >  1:2165368/1‑100 (MQ=255)
                             gCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCa                                                                       <  1:2446232/100‑1 (MQ=255)
                             gCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCa                                                                       <  2:180810/100‑1 (MQ=255)
                              cTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCAt                                                                      >  2:1205550/1‑100 (MQ=255)
                              cTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCAt                                                                      >  1:575518/1‑100 (MQ=255)
                               tGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATc                                                                     >  2:1900090/1‑100 (MQ=255)
                                 aaTTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTa                                                                   >  1:2555922/1‑100 (MQ=255)
                                    ttaaagaagTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGCCACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAggg                                                                <  2:369521/97‑1 (MQ=255)
                                    ttGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAggg                                                                <  2:1591428/100‑1 (MQ=255)
                                     tGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAgggg                                                               >  1:2575458/1‑100 (MQ=255)
                                     tGAAGAAGTCAAAAACAAAAAATAATCGCCCTCTAAACGGGTAATTATACTGACACGGGCGCATGGGAATTTCCTCTCCGCCCGTCCATTCCTCTAgggg                                                               >  1:1207659/1‑100 (MQ=255)
                                      gaagaaGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGc                                                              <  1:815076/100‑1 (MQ=255)
                                      gaagaaGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGc                                                              <  1:2357059/100‑1 (MQ=255)
                                            gTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTa                                                        >  1:632520/1‑100 (MQ=255)
                                            gTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATATAGGGGCAATTTa                                                        >  1:1283880/1‑100 (MQ=255)
                                              cAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaa                                                      >  2:419952/1‑100 (MQ=255)
                                                aaGAAAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaaa                                                    <  1:914855/100‑1 (MQ=255)
                                                 aGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaaaa                                                   >  1:2449815/1‑100 (MQ=255)
                                                    cAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGAt                                                >  2:2690904/1‑100 (MQ=255)
                                                    cAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGAt                                                >  2:1093049/1‑100 (MQ=255)
                                                    cAAAAAATAATAGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTACTCTCAGCCCGTACATTCATCTAGGGGCAATTTAAAAAAGAt                                                >  1:183189/1‑100 (MQ=255)
                                                     aaaaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATg                                               >  1:807453/1‑100 (MQ=255)
                                                      aaaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATgg                                              >  1:237204/1‑100 (MQ=255)
                                                       aaaaTAATCGCCCTATAACCGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGc                                             <  1:2113775/100‑1 (MQ=255)
                                                       aaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGc                                             >  1:476546/1‑100 (MQ=255)
                                                       aaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGc                                             <  2:723516/100‑1 (MQ=255)
                                                         aataatCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTa                                           <  1:350878/100‑1 (MQ=255)
                                                            aatCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGc                                        <  2:2283134/100‑1 (MQ=255)
                                                              tCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCaa                                      <  2:762179/100‑1 (MQ=255)
                                                               cGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCaa                                      >  1:2516669/1‑99 (MQ=255)
                                                               cGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAg                                     <  1:1313335/100‑1 (MQ=255)
                                                               cGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAg                                     <  1:2127722/100‑1 (MQ=255)
                                                                 cccTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGat                                   <  2:1948689/100‑1 (MQ=255)
                                                                 cccTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGa                                    >  1:684556/1‑99 (MQ=255)
                                                                     ataAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGattat                                <  1:1158454/99‑1 (MQ=255)
                                                                      taAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGAttatta                              <  2:1758456/100‑1 (MQ=255)
                                                                      taAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGAttatta                              >  2:205878/1‑100 (MQ=255)
                                                                      taAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGAttatta                              >  1:2653995/1‑100 (MQ=255)
                                                                        aaCGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACg                            <  2:2645210/100‑1 (MQ=255)
                                                                          cGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACgag                          >  1:825838/1‑100 (MQ=255)
                                                                            ggTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACgaga                         >  2:1780332/1‑99 (MQ=255)
                                                                             gTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAtt                       >  1:527435/1‑100 (MQ=255)
                                                                                aTTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTa                    >  2:1450567/1‑100 (MQ=255)
                                                                                 ttATACTGACACGGGCGAAGGGGAATTTTCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAg                   <  2:713993/100‑1 (MQ=255)
                                                                                  tataCTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCAGCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAgg                  >  1:1479611/1‑100 (MQ=255)
                                                                                   ataCTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGc                 <  2:2541828/100‑1 (MQ=255)
                                                                                    taCTGACACTGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCg                <  1:95383/100‑1 (MQ=255)
                                                                                    taCTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCg                <  1:276072/100‑1 (MQ=255)
                                                                                     aCTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGt               >  1:659077/1‑100 (MQ=255)
                                                                                      aatgcacGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGAATATTACGAGACTTTAGGCg                >  1:1608471/5‑98 (MQ=21)
                                                                                        tgcacGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGttt             >  2:563337/3‑99 (MQ=25)
                                                                                        tgcacGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTc            >  2:563249/3‑100 (MQ=25)
                                                                                        gACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGtt              >  1:1637225/1‑98 (MQ=255)
                                                                                        gACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGCTTc            >  1:2007277/1‑100 (MQ=255)
                                                                                         acacGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTcc           <  2:108243/100‑1 (MQ=255)
                                                                                          cacGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCa          >  1:201306/1‑100 (MQ=37)
                                                                                          cacGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCa          >  2:301105/1‑100 (MQ=255)
                                                                                           acGGGCGGAGAGGAAATTCCCCTTCGCCCGTGTATTCATCTAAGGACAATGTCAAAAAGATGGCTAAGCAATCTAATTCCGAGATTAGAGGCGTTTCCaa         >  1:1810516/1‑100 (MQ=12)
                                                                                           acGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaa         >  2:945391/1‑100 (MQ=37)
                                                                                           acGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaa         <  2:1991087/100‑1 (MQ=255)
                                                                                           acGGGCGAAGGGGAATTTCCTCTACGGCCGGGAATTAATCTGGGGGAAGTTTAAAAAAGAAGGCTTATCAAGATTATTAAGAGATTTTAAGCGtttggc          >  1:2254699/1‑96 (MQ=255)
                                                                                            cGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaa        >  2:1181839/1‑100 (MQ=37)
                                                                                            cGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaa        >  1:1714930/1‑100 (MQ=255)
                                                                                            cGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaa        <  1:2395583/100‑1 (MQ=255)
                                                                                             gggCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaaa       >  2:2672926/1‑100 (MQ=37)
                                                                                              ggCGGAGAGGAAATTCCCCTTCGCCCGTGTATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAAc      <  2:1426542/100‑1 (MQ=37)
                                                                                              ggCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAAc      >  1:318452/1‑100 (MQ=38)
                                                                                              ggCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAAc      >  2:1034515/1‑100 (MQ=255)
                                                                                               gCGAAGTGTAATTTCCTCTCCGCCCGGGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAAc      <  1:735874/99‑1 (MQ=255)
                                                                                               gCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAAc      <  1:419950/99‑1 (MQ=255)
                                                                                               gCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACa     >  2:467662/1‑100 (MQ=255)
                                                                                               gCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACa     >  1:110761/1‑100 (MQ=255)
                                                                                                cGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAg    <  2:2428468/100‑1 (MQ=255)
                                                                                                 gAAGGGGAATTTCCTCTCCGCCCGTGCAGTCATCTAGGAGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAg    <  1:166285/99‑1 (MQ=255)
                                                                                                  aaGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCTAAAACAGCg  <  2:1231855/100‑1 (MQ=255)
                                                                                                  aaGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGAGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCg  <  1:1206731/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
ACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCG  >  NC_000913/14018‑14215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: