Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,932,094 C→G 21.3% intergenic (+35/+21) purT → / ← eda phosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,932,0940CG21.3% 250.4 / 67.1 110intergenic (+35/+21)purT/edaphosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase
Reads supporting (aligned to +/- strand):  ref base C (40/45);  new base G (11/12);  total (53/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01

Only 100 of 115 total aligned reads displayed.

AGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCC  >  NC_000913/1931995‑1932193
                                                                                                   |                                                                                                   
agagTGTTGTTGACGCCATGGAACGCGCGAAGCACGACGCCGGACAGGTAAAAGTACAGGGTTAAAACCGGCAAAAAAACGCTACAAAAATGCCCGATcc                                                                                                     <  1:83377/100‑1 (MQ=255)
   gtgtTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCg                                                                                                  >  2:2008422/1‑100 (MQ=255)
     gttgttGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGAt                                                                                                >  1:1999748/1‑100 (MQ=255)
     gttgttGACGCCATTGAACGCGCGAAACACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGAt                                                                                                >  2:1774996/1‑100 (MQ=255)
       tgttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATcn                                                                                              <  2:2134891/100‑2 (MQ=255)
        gttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCgg                                                                                             <  2:2152387/100‑1 (MQ=255)
        gttgACGCCAGTGAACGCGCGAAGCACGCGGCCAGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCgg                                                                                             <  1:1496138/100‑1 (MQ=255)
         ttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCggg                                                                                            <  1:1329254/100‑1 (MQ=255)
          tgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGc                                                                                           >  2:2574693/1‑100 (MQ=255)
           gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCa                                                                                          <  2:2236136/100‑1 (MQ=255)
           gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCa                                                                                          >  2:1189849/1‑100 (MQ=255)
           gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGGAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGc                                                                                           <  1:387997/99‑1 (MQ=255)
            aCGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAt                                                                                         <  2:2333794/100‑1 (MQ=39)
            aCGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAt                                                                                         <  2:2028599/100‑1 (MQ=39)
            aCGCCATTGAACGCGCGAAGCACGACGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAt                                                                                         <  1:2763371/100‑1 (MQ=39)
             cGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAtt                                                                                        <  2:1263242/100‑1 (MQ=39)
             cGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAtt                                                                                        <  2:2812234/100‑1 (MQ=39)
             cGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtt                                                                                        <  2:1926132/100‑1 (MQ=255)
              gctaTTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAttt                                                                                       >  2:408116/4‑100 (MQ=255)
              gacATTGAACGCGATAAGCACGCCGCAGAACAAGTAAAATTACAGGGTTAAAGCCGGAAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAtt                                                                                        <  1:1609740/97‑1 (MQ=19)
               cctatGAACTCGAGAAGCACGCCGCTGGATAGTTAAAAGAACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAtttt                                                                                      <  1:1437003/96‑1 (MQ=25)
               ccATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtttt                                                                                      >  1:127672/1‑100 (MQ=255)
                cATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTg                                                                                     >  1:2478503/1‑100 (MQ=255)
                 aTTGAACGCCCGAAGCACGCCAGCAGAGAGGGATAATTACAGGGTTAAACTCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAttt                                                                                       <  1:2666390/97‑1 (MQ=21)
                       cgcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTa                                                                              >  2:648433/1‑100 (MQ=255)
                        gcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAc                                                                             >  2:1214084/1‑100 (MQ=255)
                        gcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTGAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAc                                                                             <  2:2415146/100‑1 (MQ=255)
                          gcgAAGCACGCCACCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACa                                                                            >  2:1916837/1‑99 (MQ=255)
                           cgcaGCACGCCGCCAGACAGGTAAAAGTACAGAATTAAACCCGTCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAc                                                                             <  1:1602100/94‑1 (MQ=255)
                            gAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCt                                                                         >  2:2913344/1‑100 (MQ=255)
                                cACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgc                                                                     >  1:1567430/1‑100 (MQ=255)
                                cACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAg                                                                      >  2:649073/1‑99 (MQ=255)
                                 aCGCCGCCGGACAGGTAAAAGTACAGGGTTAACCCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAg                                                                      <  1:1722104/98‑1 (MQ=255)
                                 aCGCCGCCGGACAGGTAAAAGTACAGGGTTAAACTCGGCAAAAAAACGCTACAAAAATGCAAGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgc                                                                     >  1:552603/1‑99 (MQ=255)
                                 aCGCCGCCGGACAGGTAAAAGTACAGGGTTAAACTCGGCAAAAAAACGCTACAAAAATGACCGATCTTCGATCGGGCATTTTGACTTTTACAGCTTAgcg                                                                    >  2:1762350/1‑100 (MQ=255)
                                 aCGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgc                                                                     <  1:1325799/99‑1 (MQ=255)
                                  cgccgcCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgcgc                                                                   <  2:2395094/100‑1 (MQ=255)
                                  cgccgcCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgcgc                                                                   >  1:883906/1‑100 (MQ=255)
                                   gccgccGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGcc                                                                  <  2:1661996/100‑1 (MQ=255)
                                   gccgccGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGcc                                                                  <  2:2558704/100‑1 (MQ=255)
                                   gccgccGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGcc                                                                  <  2:116537/100‑1 (MQ=255)
                                   gccgccGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGcc                                                                  <  1:416954/100‑1 (MQ=255)
                                    ccgccgGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCt                                                                 >  2:2362770/1‑100 (MQ=255)
                                        cgGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTc                                                               <  1:770555/98‑1 (MQ=255)
                                         ggACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTAc                                                            <  2:695334/100‑1 (MQ=255)
                                         ggACAGGTAAAAGTACAGGGTAAAAAAAAAAAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTa                                                             <  1:2112186/99‑1 (MQ=255)
                                          gACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACa                                                           <  2:654670/100‑1 (MQ=255)
                                          gACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAAAGCTTAGCGCCTTCTAc                                                            <  1:936510/99‑1 (MQ=255)
                                             aGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCt                                                        <  1:2192374/100‑1 (MQ=255)
                                              ggTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCtt                                                       >  2:1209877/1‑100 (MQ=255)
                                              ggTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCtt                                                       >  2:1703281/1‑100 (MQ=255)
                                                tAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCa                                                     >  2:1185179/1‑100 (MQ=255)
                                                tAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCa                                                     >  1:2782539/1‑100 (MQ=255)
                                                tAAAAGTACAGGGTTAAACCCGGCAAAAAAAAGCTACAAAAATGCATGATTATCGATCGGGCATTTTGACATTTACAGCTTATCGCCTTCTACAGCtt                                                       >  1:1822335/1‑98 (MQ=255)
                                                    aGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAcgcg                                                 >  2:312366/1‑100 (MQ=255)
                                                         aGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGc                                            <  2:556360/100‑1 (MQ=255)
                                                            gTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTa                                         >  2:2589928/1‑100 (MQ=255)
                                                                aaCCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTaa                                     >  2:880842/1‑100 (MQ=255)
                                                                aaCCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTaa                                     <  1:2123615/100‑1 (MQ=255)
                                                                  cccGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTa                                      <  2:2472175/97‑1 (MQ=255)
                                                                  cccGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATg                                   >  2:718114/1‑100 (MQ=255)
                                                                   ccGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGc                                  >  2:2279403/1‑100 (MQ=255)
                                                                   ccGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGc                                  <  1:1507771/100‑1 (MQ=255)
                                                                      gCAAAACAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGt                               >  1:2907307/1‑100 (MQ=255)
                                                                      gCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGt                               <  2:2478756/100‑1 (MQ=255)
                                                                       cAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTc                              >  2:1259162/1‑100 (MQ=255)
                                                                        aaaaaaaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCg                             <  2:392837/100‑1 (MQ=255)
                                                                         aaaaaaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGt                            >  2:194549/1‑100 (MQ=255)
                                                                         aaaaaaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGt                            <  2:883909/100‑1 (MQ=255)
                                                                           aaaaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTaa                          <  2:2510537/100‑1 (MQ=255)
                                                                            tgtaaaagtcaaaaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAAt                         >  2:1442216/11‑100 (MQ=25)
                                                                            aaaCGTTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAAt                         <  2:1289407/100‑1 (MQ=255)
                                                                                gCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGcc                     >  1:544371/1‑100 (MQ=255)
                                                                                gCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGcc                     <  1:2792142/100‑1 (MQ=255)
                                                                                 cTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGccc                    <  2:843238/100‑1 (MQ=255)
                                                                                  tACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCg                   <  2:76510/100‑1 (MQ=255)
                                                                                  tACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCg                   >  2:822959/1‑100 (MQ=255)
                                                                                  agtcaaaaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCg                   >  1:1327298/5‑100 (MQ=38)
                                                                                   gtcaaaaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTTATCGCCCGc                  >  1:1605754/4‑100 (MQ=37)
                                                                                    tcaaaaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCt                 >  2:2759154/3‑100 (MQ=38)
                                                                                     caaaaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCtt                >  2:2008595/2‑100 (MQ=38)
                                                                                     caaaaTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTTAAGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCtt                >  1:1684228/2‑100 (MQ=255)
                                                                                      aaaaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTc               >  2:1943911/1‑100 (MQ=39)
                                                                                      aaaaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTc               >  1:2101698/1‑100 (MQ=38)
                                                                                       aaaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCTCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTcc              >  2:1051649/1‑100 (MQ=38)
                                                                                       aaaTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTcc              <  2:1120340/100‑1 (MQ=255)
                                                                                        aaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCa             >  1:107550/1‑100 (MQ=255)
                                                                                         aTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAg            >  2:2511247/1‑100 (MQ=39)
                                                                                         aTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAg            <  1:194548/100‑1 (MQ=255)
                                                                                         aTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAg            >  1:1748680/1‑100 (MQ=255)
                                                                                          tGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAgc           <  2:520728/100‑1 (MQ=255)
                                                                                          tGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAgc           <  2:703986/100‑1 (MQ=255)
                                                                                          tGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAgc           <  2:1734629/100‑1 (MQ=255)
                                                                                            cccGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAgcgc         >  1:484027/1‑100 (MQ=255)
                                                                                             ccGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCa        <  2:931537/100‑1 (MQ=255)
                                                                                               gATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATc      >  2:2051739/1‑100 (MQ=255)
                                                                                                  ccTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTGTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTg    <  1:1927936/99‑1 (MQ=255)
                                                                                                  ccTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGc   <  1:1185174/100‑1 (MQ=255)
                                                                                                   cTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGcc  >  2:318729/1‑100 (MQ=255)
                                                                                                   cTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGcc  >  1:1190456/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
AGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCC  >  NC_000913/1931995‑1932193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: