Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,945,839 G→A 100% I46I (ATC→ATT ruvA ← component of RuvABC resolvasome, regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,945,8390GA88.6% 244.8 / 18.3 95I46I (ATC→ATTruvAcomponent of RuvABC resolvasome, regulatory subunit
Reads supporting (aligned to +/- strand):  ref base G (4/7);  new base A (45/38);  total (50/45)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGG  >  NC_000913/1945742‑1945935
                                                                                                 |                                                                                                
tGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAAt                                                                                                <  2:1905898/100‑1 (MQ=255)
 ggTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATc                                                                                               <  2:773990/100‑1 (MQ=255)
   ttttGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGc                                                                                             >  2:409525/1‑100 (MQ=255)
      tGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTc                                                                                          >  1:1036783/1‑100 (MQ=255)
       gATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTcc                                                                                         <  2:2847549/100‑1 (MQ=255)
       gATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTcc                                                                                         <  1:111938/100‑1 (MQ=255)
        aTCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCt                                                                                        >  2:1707742/1‑100 (MQ=255)
        aTCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCt                                                                                        <  2:568232/100‑1 (MQ=255)
        aTCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCt                                                                                        >  1:2357427/1‑100 (MQ=255)
         tCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTg                                                                                       >  2:627001/1‑100 (MQ=255)
          cAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTg                                                                                       >  1:2095662/1‑99 (MQ=255)
             ctctTTGAACAATGTGCGCTCTTGTTTATTGTTAAAGCCATACAGCAGTTGCGCGTCTTCACGCACCACAAAGTTGGTGAAAACAATCGCTTCCTGGcc                                                                                    <  1:2271998/99‑1 (MQ=255)
             ctctTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGAccc                                                                                   >  1:909824/1‑100 (MQ=255)
             ctctTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGAccc                                                                                   <  1:637208/100‑1 (MQ=255)
              tctTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGAccc                                                                                   >  1:2899551/1‑99 (MQ=255)
              tctTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCg                                                                                  >  2:1374599/1‑100 (MQ=255)
              tctTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCg                                                                                  >  2:2137523/1‑100 (MQ=255)
               ctTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACGATCGCTTCCTGACCCGc                                                                                 >  1:1486526/1‑100 (MQ=255)
               ctTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGc                                                                                 >  2:2841679/1‑100 (MQ=255)
                 ttGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTCGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACGATCGCTTCCTGACCCGCt                                                                                <  1:782336/99‑1 (MQ=255)
                   gAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCa                                                                             >  1:96173/1‑100 (MQ=255)
                    aaCAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAg                                                                            <  2:918077/100‑1 (MQ=255)
                      cAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACGATCGCTTCCTGACCCGCTTCAggg                                                                          <  1:1880250/100‑1 (MQ=255)
                      cAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACGATCGCTTCCTGACCCGCTTCAggg                                                                          >  1:2527893/1‑100 (MQ=255)
                      cAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAggg                                                                          <  2:553673/100‑1 (MQ=255)
                      cAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAggg                                                                          <  2:684954/100‑1 (MQ=255)
                      cAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAggg                                                                          <  2:1887155/100‑1 (MQ=255)
                       aaTGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGa                                                                         >  2:2588818/1‑100 (MQ=255)
                        aTGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAg                                                                        <  2:179349/100‑1 (MQ=255)
                        aTGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAg                                                                        <  1:2555387/100‑1 (MQ=255)
                         tgtgCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGt                                                                       <  1:1597629/100‑1 (MQ=255)
                         tgtgCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGt                                                                       <  2:1328719/100‑1 (MQ=255)
                          gtgCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGtt                                                                      <  2:1303095/100‑1 (MQ=255)
                          gtgCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGtt                                                                      <  2:496301/100‑1 (MQ=255)
                          gtgCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGtt                                                                      >  1:682450/1‑100 (MQ=255)
                           tgCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTc                                                                     <  2:2211435/100‑1 (MQ=255)
                           tgCGCTCTTGTTTATTGTTAAAACCGCGCAACACGTTCGCGTCTTCAAGCACCACCAAGTGGGGGAAAACCATCCCTTCCCGACCCGCTTTAGGGAGTTc                                                                     >  1:583016/1‑100 (MQ=255)
                              gcTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACACTCGCTTCCTGACCCGCTTCAGGGAGTTCATa                                                                  >  1:262772/1‑100 (MQ=255)
                               ctctTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATaa                                                                 >  2:2794186/1‑100 (MQ=255)
                               ctctTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATaa                                                                 >  2:1793539/1‑100 (MQ=255)
                               ctctTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATaa                                                                 >  2:1168961/1‑100 (MQ=255)
                                tctTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATaaa                                                                <  2:938654/100‑1 (MQ=255)
                                    gTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACa                                                            <  2:2298467/100‑1 (MQ=255)
                                    gTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACa                                                            >  2:2425261/1‑100 (MQ=255)
                                      ttATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTTACCCGCTTCAGGGAGTTCATAAAAACAgg                                                          >  1:697675/1‑100 (MQ=255)
                                       tATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAGCAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGt                                                         >  1:1866949/1‑100 (MQ=255)
                                       tATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGt                                                         <  1:68267/100‑1 (MQ=255)
                                        aTTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTc                                                        <  2:1103683/100‑1 (MQ=255)
                                           gTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATc                                                     >  1:1511813/1‑100 (MQ=255)
                                           gTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATc                                                     >  2:804247/1‑100 (MQ=255)
                                           gTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATc                                                     >  2:401421/1‑100 (MQ=255)
                                           gTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATc                                                     >  2:2794232/1‑100 (MQ=255)
                                            ttAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCg                                                    <  2:260305/100‑1 (MQ=255)
                                             tAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCgg                                                   >  2:1701665/1‑100 (MQ=255)
                                             tAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCgg                                                   >  1:2537073/1‑100 (MQ=255)
                                             tAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCgg                                                   >  2:2246707/1‑100 (MQ=255)
                                              aaaaCCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGc                                                  >  2:2265370/1‑100 (MQ=255)
                                              aaaaCCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGc                                                  <  2:1053215/100‑1 (MQ=255)
                                                 aCCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTTAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCata                                               <  2:1513346/100‑1 (MQ=255)
                                                 aCCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCata                                               <  2:2400719/100‑1 (MQ=255)
                                                 aCCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCata                                               <  2:2915894/100‑1 (MQ=255)
                                                     tACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCa                                           >  1:1436600/1‑100 (MQ=255)
                                                     tACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCa                                           >  2:1461182/1‑100 (MQ=255)
                                                     tACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGGTCATAAAAACAGGTCATCGGCATATGCa                                           >  2:879511/1‑100 (MQ=255)
                                                       cagcagTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACt                                         <  2:1866958/100‑1 (MQ=255)
                                                       cagcagTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACt                                         >  2:608476/1‑100 (MQ=255)
                                                        agcagTTACGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACtt                                        <  1:1133991/100‑1 (MQ=255)
                                                           agTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCAt                                     <  1:1098293/100‑1 (MQ=255)
                                                             ttGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAg                                   <  2:2790511/100‑1 (MQ=255)
                                                               gcgcGTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGcc                                 >  1:567075/1‑100 (MQ=255)
                                                                cgcgTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGcc                                 <  1:676690/99‑1 (MQ=255)
                                                                cgcgTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCt                                >  2:1232960/1‑100 (MQ=255)
                                                                  cgTCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTAc                              >  1:2901969/1‑100 (MQ=255)
                                                                    tattCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTAcgc                            >  2:1969266/3‑100 (MQ=255)
                                                                    tCTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTAcgc                            >  2:914522/1‑100 (MQ=255)
                                                                     cTTCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTAcgcc                           <  2:2832671/100‑1 (MQ=255)
                                                                      ttCACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTAcgccg                          >  1:2074865/1‑100 (MQ=255)
                                                                        cACGCACCACAAAGTGGGTGAAAACGATCGCTTCCTGTCCCGCTTCAGAAAGTTAATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACgccgcc                        <  1:2672077/100‑1 (MQ=255)
                                                                        cACGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACgccgcc                        >  2:1446407/1‑100 (MQ=255)
                                                                         aCGCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGccc                       <  1:2147701/100‑1 (MQ=255)
                                                                           gCACCACAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCAc                     <  1:2582765/100‑1 (MQ=255)
                                                                            caccacAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACt                    >  2:567844/1‑100 (MQ=255)
                                                                             accacAAAGTGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACtt                   >  2:1388937/1‑100 (MQ=255)
                                                                             accacAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACtt                   <  2:1055901/100‑1 (MQ=255)
                                                                              ccacAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTc                  >  2:928128/1‑100 (MQ=255)
                                                                              ccacAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTc                  >  2:1641556/1‑100 (MQ=255)
                                                                              ccacAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTc                  <  1:1629690/100‑1 (MQ=255)
                                                                              ccacAAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTc                  <  1:439313/100‑1 (MQ=255)
                                                                               cacaAAGTGGGTGAAAACAATCGCTTCCTGACCCGCTGCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCa                 >  1:1561642/1‑100 (MQ=255)
                                                                                acaAAGTGGGTGAAAACAATCGCTTCCTGACACGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCa                 <  1:1234852/99‑1 (MQ=255)
                                                                                 caAAGTGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAAt               <  2:579968/100‑1 (MQ=255)
                                                                                      tgcgTGAACACAATGGCTACCCGACCCGCGACATGAAGGTAACAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAac           <  1:1929028/96‑1 (MQ=255)
                                                                                        ggTGAAAACAATCGCTTCCTGACCCACTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACAcc        >  1:163824/1‑100 (MQ=255)
                                                                                         gTGAAAACAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCa       >  2:92468/1‑100 (MQ=255)
                                                                                              aaCAATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACATCAGCggg  <  2:1525164/100‑1 (MQ=255)
                                                                                                 |                                                                                                
TGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGG  >  NC_000913/1945742‑1945935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: