Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,143,720 C→G 15.7% P152R (CCT→CGT)  yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,143,7200CG15.7% 329.4 / 45.5 119P152R (CCT→CGT) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (49/51);  new base G (12/7);  total (61/58)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

Only 100 of 119 total aligned reads displayed.

CTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCTGCTGC  >  NC_000913/2143626‑2143815
                                                                                              |                                                                                               
cTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCa                                                                                            >  2:1629884/1‑100 (MQ=255)
 tGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAg                                                                                           <  2:2519949/100‑1 (MQ=255)
 tGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAg                                                                                           <  1:1724517/100‑1 (MQ=255)
 tGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAg                                                                                           <  1:1629878/100‑1 (MQ=255)
  ggCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAgg                                                                                          >  2:496041/1‑100 (MQ=255)
  ggCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAgg                                                                                          >  1:1579160/1‑100 (MQ=255)
  ggCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTATCCTGCTGACGCCCGGAGACGATCCTCTCAgg                                                                                          >  1:1129687/1‑100 (MQ=255)
   gCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAggg                                                                                         <  1:2603356/100‑1 (MQ=255)
    cTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCTGAGAGGATCGTCTCCGGGc                                                                                        <  1:815857/100‑1 (MQ=255)
    cTGACTGGCTGCGTCTGGCACTCGGCAGCGCCAATGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTACTGACGCCCGGAGACGATCCTCTCAGGGc                                                                                        >  1:1751596/1‑100 (MQ=255)
     tGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCTGAGAGGATCGTCTCCGGGcg                                                                                       <  1:1150179/100‑2 (MQ=255)
     tGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCa                                                                                       <  2:1583033/100‑1 (MQ=255)
      gACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCTGAGAGGATCGTCTCCGGGcg                                                                                       <  1:1857703/99‑2 (MQ=39)
        cTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCAttt                                                                                    >  1:122644/1‑100 (MQ=255)
         tGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCCCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCTGAGAGGATCGTCTCCGGGcgtcag                                                                                   <  1:239646/100‑6 (MQ=37)
          ggCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCAttttt                                                                                  >  2:1303927/1‑100 (MQ=255)
          ggCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCAttttt                                                                                  >  1:460152/1‑100 (MQ=255)
           gCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCTGAGAGGATCGTCTCCGGGcgtcagca                                                                                 <  1:2632377/100‑8 (MQ=37)
           gCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTg                                                                                 <  2:468852/100‑1 (MQ=255)
            cggCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGa                                                                                <  1:148373/98‑1 (MQ=255)
            cTGCGTCTGGTACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGa                                                                                >  2:2417984/1‑100 (MQ=255)
            cTGCGTCTGGCACTCGGCAGCGGCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCTGAGAGGATCGTCTCCGGGcgtcagcag                                                                                <  1:1789111/100‑9 (MQ=25)
            cTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTg                                                                                 >  1:835205/1‑99 (MQ=255)
            cTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGa                                                                                <  2:2611210/100‑1 (MQ=255)
            cTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGa                                                                                <  1:111964/100‑1 (MQ=255)
            cTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTCCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGa                                                                                >  2:883824/1‑100 (MQ=255)
                gTCTGGCTCTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATg                                                                            <  2:43867/100‑1 (MQ=255)
                gTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATg                                                                            <  2:2771561/100‑1 (MQ=255)
                gTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATg                                                                            >  2:2038990/1‑100 (MQ=255)
                 tCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATgg                                                                           <  1:504271/100‑1 (MQ=255)
                  cTGGCACTCGGTAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATggg                                                                          <  2:2589153/100‑1 (MQ=255)
                  cTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGTGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATggg                                                                          <  2:2045568/100‑1 (MQ=255)
                    ggCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTa                                                                        >  2:1680895/1‑100 (MQ=255)
                     gCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTAc                                                                       >  1:838445/1‑100 (MQ=255)
                     gCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTAc                                                                       <  2:907897/100‑1 (MQ=255)
                      cACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACt                                                                      >  2:1541836/1‑100 (MQ=255)
                        cTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGt                                                                    >  2:1178395/1‑100 (MQ=255)
                         tCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTc                                                                   >  2:721176/1‑100 (MQ=255)
                         tCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTc                                                                   >  2:2261298/1‑100 (MQ=255)
                          cGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCa                                                                  >  2:2235229/1‑100 (MQ=255)
                          cGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCa                                                                  >  2:2384559/1‑100 (MQ=255)
                            gCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGCTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGa                                                                >  2:2416302/1‑100 (MQ=255)
                             cAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGaa                                                               <  1:2306106/100‑1 (MQ=255)
                               gcgcCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATc                                                             <  2:460155/100‑1 (MQ=255)
                               gcgcCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATc                                                             <  2:2776105/100‑1 (MQ=255)
                                cgcCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATcc                                                            >  1:1635320/1‑100 (MQ=255)
                                cgcCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATcc                                                            >  2:1457840/1‑100 (MQ=255)
                                 gcCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCa                                                           >  2:970818/1‑100 (MQ=255)
                                 gcCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCa                                                           >  2:970017/1‑100 (MQ=255)
                                  ccATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCAt                                                          >  2:403060/1‑100 (MQ=255)
                                     ttGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCgg                                                       <  2:1164668/100‑1 (MQ=255)
                                      tGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCTGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGgc                                                      <  1:1796995/100‑1 (MQ=255)
                                      tGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGgc                                                      <  2:732227/100‑1 (MQ=255)
                                         tCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCAGCGGCGct                                                   >  1:1560654/1‑100 (MQ=255)
                                          ccGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATTTGGGTACTGTCAGAATCCATCGGCGctc                                                  >  1:1018631/1‑100 (MQ=255)
                                            gCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTg                                                <  1:2415238/100‑1 (MQ=255)
                                              ctctGTTGGGGGGTGTCCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGc                                              <  1:883821/100‑1 (MQ=255)
                                               tctGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGGTATGGGTACTGTCAGAATCCATCGGCGCTCTGGCa                                             <  2:2334780/100‑1 (MQ=255)
                                                ctGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGCCGATCCTCTCAGGGCATTTTTGATATGGGTAGTGTCAGAATCCATCGGCGCTATGGc                                              >  1:1124104/1‑98 (MQ=255)
                                                ctGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCAc                                            >  1:1185041/1‑100 (MQ=255)
                                                ctGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCAc                                            >  2:705271/1‑100 (MQ=255)
                                                  gTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTg                                          >  1:339220/1‑100 (MQ=255)
                                                   ttGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTgg                                         <  2:178603/100‑1 (MQ=255)
                                                    tGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGt                                        >  2:2485505/1‑100 (MQ=255)
                                                      gggggTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGc                                      <  1:970014/100‑1 (MQ=255)
                                                      gggggTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGc                                      <  1:970815/100‑1 (MQ=255)
                                                       ggggTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTg                                       <  1:88987/98‑1 (MQ=255)
                                                       ggggTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGcc                                     <  2:2428034/100‑1 (MQ=255)
                                                           tgtTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTg                                 >  1:1096189/1‑100 (MQ=255)
                                                            gtTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTgg                                <  1:2591696/100‑1 (MQ=255)
                                                            gtTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTgg                                <  2:1366165/100‑1 (MQ=255)
                                                               cTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGAt                             <  1:496038/100‑1 (MQ=255)
                                                               cTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGAt                             <  2:855556/100‑1 (MQ=255)
                                                                  gTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGt                          <  2:1373341/100‑1 (MQ=255)
                                                                      tatcaaaaatgCCCTGAGAGGATCGTCTCCAGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTAtt                      >  1:902653/11‑100 (MQ=25)
                                                                      tCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTAtt                      <  2:1903013/100‑1 (MQ=255)
                                                                       ccTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTAttt                     >  2:111731/1‑100 (MQ=255)
                                                                       ccTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTAttt                     <  2:227789/100‑1 (MQ=255)
                                                                       ccTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTAttt                     <  1:234749/100‑1 (MQ=255)
                                                                       ccTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTAttt                     >  1:964884/1‑100 (MQ=255)
                                                                       atcaaaaatgCCCTGAGAGGATCGTCTCCGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTAttt                     >  2:561927/10‑100 (MQ=25)
                                                                        ctgctgACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTa                    >  2:1159265/1‑100 (MQ=255)
                                                                         tgctgACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTaa                   <  1:1752356/100‑1 (MQ=255)
                                                                         caaaaatgCCCTGAGAGGATCGTCTCCGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTaa                   >  1:1335310/8‑100 (MQ=37)
                                                                         caaaaatgCCCTGAGAGGATCGTCTCCGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTaa                   >  2:1615867/8‑100 (MQ=37)
                                                                          aaaaatgCCCTGAGAGGATCGTCTCCGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTaaa                  >  2:2402562/7‑100 (MQ=37)
                                                                            tgACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAAcc                <  1:1030513/100‑1 (MQ=255)
                                                                            tgACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAAcc                <  2:556023/100‑1 (MQ=255)
                                                                             aatgCCCTGAGAGGATCGTCTCCGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACca               >  2:2264758/4‑100 (MQ=38)
                                                                             aatgCCCTGAGAGGAACGTCTCCGGGCATTTTTGATATGGGAACTGTCAGAATCCATCGGCGCTCTGGAACTGGTGCCGCTGGGATTGTTATTTAAAcc                >  1:946205/4‑99 (MQ=255)
                                                                              aCGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACcac              <  2:1063200/100‑1 (MQ=255)
                                                                               tgCCCTGAGAGGATCGTCTCCGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACcaca             >  1:1195491/2‑100 (MQ=255)
                                                                                gCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCacac            <  1:679157/100‑1 (MQ=255)
                                                                                 cccTGAGAGGATCGTCTCCGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACt           >  1:2109759/1‑100 (MQ=255)
                                                                                 cccGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACt           >  2:1378597/1‑100 (MQ=255)
                                                                                   cGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTa          <  1:705268/99‑1 (MQ=255)
                                                                                       gaCGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATctgc     <  1:976005/100‑1 (MQ=255)
                                                                                         cGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATctgctg   <  1:322202/100‑1 (MQ=255)
                                                                                         cGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATctgctg   >  2:181031/1‑100 (MQ=255)
                                                                                          gATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCtgctgc  <  2:212843/100‑1 (MQ=255)
                                                                                              |                                                                                               
CTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCTGCTGC  >  NC_000913/2143626‑2143815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: