Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,143,725 A→C 15.3% R154R (AGG→CGG)  yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,143,7250AC15.3% 293.7 / 47.6 119R154R (AGG→CGG) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (50/50);  new base C (11/7);  total (61/58)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01

Only 100 of 122 total aligned reads displayed.

CTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCTGCTGCGCCATCGCA  >  NC_000913/2143626‑2143824
                                                                                                   |                                                                                                   
cTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCa                                                                                                     >  1:988765/1‑100 (MQ=255)
cTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCa                                                                                                     >  2:1629884/1‑100 (MQ=255)
 tGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAg                                                                                                    <  1:1724517/100‑1 (MQ=255)
 tGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAg                                                                                                    <  2:2519949/100‑1 (MQ=255)
  ggCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAgg                                                                                                   >  2:496041/1‑100 (MQ=255)
  ggCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAgg                                                                                                   >  1:1579160/1‑100 (MQ=255)
    cTGACTGGCTGCGTCTGGCACTCGGCAGCGCCAATGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTACTGACGCCCGGAGACGATCCTCTCAGGGc                                                                                                 >  1:1751596/1‑100 (MQ=255)
     tGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCTGAGAGGATCGTCTCCGGGcg                                                                                                <  1:1150179/100‑2 (MQ=255)
     tGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCTGAGAGGATCGTCTCCGGGcg                                                                                                <  2:527051/100‑2 (MQ=255)
     tGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCa                                                                                                <  2:1583033/100‑1 (MQ=255)
      gACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCTGAGAGGATCGTCTCCGGGcg                                                                                                <  1:1857703/99‑2 (MQ=39)
        cTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCAttt                                                                                             >  1:122644/1‑100 (MQ=255)
         tGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCCCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCTGAGAGGATCGTCTCCGGGcgtcag                                                                                            <  1:239646/100‑6 (MQ=37)
          ggatgggTCTGGCAGACGGCAGCGCCATTGTTACGCCTCTGTTGGGGGGTGTGCTGGTTGTTCTGCTTACGCACAGGGACGTTCGCCTCAGGGCGttttt                                                                                           >  1:1957527/7‑100 (MQ=255)
          ggCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCAttttt                                                                                           >  2:1303927/1‑100 (MQ=255)
           gCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCTGAGAGGATCGTCTCCGGGcgtcagca                                                                                          <  1:2632377/100‑8 (MQ=37)
           gCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTg                                                                                          <  2:468852/100‑1 (MQ=255)
            cggCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGa                                                                                         <  1:148373/98‑1 (MQ=255)
            cTGCGTCTGGTACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGa                                                                                         >  2:2417984/1‑100 (MQ=255)
            cTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTg                                                                                          >  1:835205/1‑99 (MQ=255)
            cTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGa                                                                                         <  2:2611210/100‑1 (MQ=255)
            cTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGa                                                                                         <  1:111964/100‑1 (MQ=255)
            cTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTCCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGa                                                                                         >  2:883824/1‑100 (MQ=255)
               cGTCTGGCACTCGGCCGCGCCATTGGTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGatat                                                                                      <  1:791928/100‑1 (MQ=255)
                gTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATg                                                                                     <  2:2771561/100‑1 (MQ=255)
                gTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATg                                                                                     >  2:2038990/1‑100 (MQ=255)
                 tCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATgg                                                                                    <  1:504271/100‑1 (MQ=255)
                  cTGGCACTCGGTAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATggg                                                                                   <  2:2589153/100‑1 (MQ=255)
                  cTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGTGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATggg                                                                                   <  2:2045568/100‑1 (MQ=255)
                   tGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGt                                                                                  <  1:249923/100‑1 (MQ=255)
                    ggCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTa                                                                                 >  2:375940/1‑100 (MQ=255)
                    ggCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTa                                                                                 >  2:1680895/1‑100 (MQ=255)
                     gCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTAc                                                                                >  1:838445/1‑100 (MQ=255)
                     gCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTAc                                                                                <  2:907897/100‑1 (MQ=255)
                      cACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACt                                                                               >  2:1541836/1‑100 (MQ=255)
                        cTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGt                                                                             >  2:1178395/1‑100 (MQ=255)
                         tCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTc                                                                            >  2:2261298/1‑100 (MQ=255)
                         tCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTc                                                                            >  2:721176/1‑100 (MQ=255)
                          cGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCa                                                                           >  2:2384559/1‑100 (MQ=255)
                            gCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGCTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGa                                                                         >  2:2416302/1‑100 (MQ=255)
                             cAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGaa                                                                        >  2:1797003/1‑100 (MQ=255)
                               gcgcCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATc                                                                      <  2:2776105/100‑1 (MQ=255)
                               gcgcCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATc                                                                      <  2:460155/100‑1 (MQ=255)
                                cgcCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATcc                                                                     >  1:1635320/1‑100 (MQ=255)
                                cgcCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATcc                                                                     >  2:1457840/1‑100 (MQ=255)
                                 gcCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCa                                                                    >  2:970818/1‑100 (MQ=255)
                                 gcCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCa                                                                    >  2:970017/1‑100 (MQ=255)
                                  ccATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCAt                                                                   >  2:403060/1‑100 (MQ=255)
                                      tGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCTGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGgc                                                               <  1:1796995/100‑1 (MQ=255)
                                      tGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGgc                                                               <  2:732227/100‑1 (MQ=255)
                                         tCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCAGCGGCGct                                                            >  1:1560654/1‑100 (MQ=255)
                                          ccGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATTTGGGTACTGTCAGAATCCATCGGCGctc                                                           >  1:1018631/1‑100 (MQ=255)
                                            gCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTg                                                         <  1:2415238/100‑1 (MQ=255)
                                              ctctGTTGGGGGGTGTCCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGc                                                       <  1:883821/100‑1 (MQ=255)
                                                ctGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGCCGATCCTCTCAGGGCATTTTTGATATGGGTAGTGTCAGAATCCATCGGCGCTATGGc                                                       >  1:1124104/1‑98 (MQ=255)
                                                ctGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCAc                                                     >  2:705271/1‑100 (MQ=255)
                                                ctGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCAc                                                     >  1:1185041/1‑100 (MQ=255)
                                                  gTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTg                                                   >  1:339220/1‑100 (MQ=255)
                                                   ttGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTgg                                                  <  2:178603/100‑1 (MQ=255)
                                                    tGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGt                                                 >  2:2485505/1‑100 (MQ=255)
                                                     ggggggTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGt                                                 >  2:1261299/1‑99 (MQ=255)
                                                     ggggggTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTg                                                >  2:2359086/1‑100 (MQ=255)
                                                      gggggTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGc                                               <  1:970815/100‑1 (MQ=255)
                                                      gggggTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGc                                               <  1:970014/100‑1 (MQ=255)
                                                       ggggTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTg                                                <  1:88987/98‑1 (MQ=255)
                                                           tgtTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTg                                          >  1:1096189/1‑100 (MQ=255)
                                                            gtTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTgg                                         <  2:1366165/100‑1 (MQ=255)
                                                            gtTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTgg                                         <  1:2591696/100‑1 (MQ=255)
                                                               cTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGAt                                      <  1:496038/100‑1 (MQ=255)
                                                                  gTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGt                                   <  2:1373341/100‑1 (MQ=255)
                                                                   ttGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGt                                   >  1:1722457/1‑99 (MQ=255)
                                                                      tCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTAtt                               <  2:1903013/100‑1 (MQ=255)
                                                                       ccTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTAttt                              <  1:234749/100‑1 (MQ=255)
                                                                       ccTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTAttt                              >  2:111731/1‑100 (MQ=255)
                                                                       ccTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTAttt                              >  1:964884/1‑100 (MQ=255)
                                                                       ccTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTAttt                              <  2:227789/100‑1 (MQ=255)
                                                                       atcaaaaatgCCCTGAGAGGATCGTCTCCGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTAttt                              >  2:561927/10‑100 (MQ=25)
                                                                        ctgctgACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTa                             >  2:1159265/1‑100 (MQ=255)
                                                                         tgctgACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTaa                            <  1:1752356/100‑1 (MQ=255)
                                                                         tgctgACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTaa                            <  2:1512391/100‑1 (MQ=255)
                                                                         caaaaatgCCCTGAGAGGATCGTCTCCGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGTGATTGTTATTTaa                            >  1:1459372/8‑100 (MQ=25)
                                                                          gctgACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTaaa                           >  2:2446344/1‑100 (MQ=255)
                                                                          aaaaatgCCCTGAGAGGATCGTCTCCGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTaaa                           >  2:790248/7‑100 (MQ=37)
                                                                          aaaaatgCCCTGAGAGGATCGTCTCCGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTaaa                           >  2:2402562/7‑100 (MQ=37)
                                                                            tgACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAAcc                         <  1:1030513/100‑1 (MQ=255)
                                                                             aatgCCCTGAGAGGATCGTCTCCGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACca                        >  2:2264758/4‑100 (MQ=38)
                                                                             aatgCCCTGAGAGGAACGTCTCCGGGCATTTTTGATATGGGAACTGTCAGAATCCATCGGCGCTCTGGAACTGGTGCCGCTGGGATTGTTATTTAAAcc                         >  1:946205/4‑99 (MQ=255)
                                                                              aCGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACcac                       <  2:1063200/100‑1 (MQ=255)
                                                                               tgCCCTGAGAGGATCGTCTCCGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACcaca                      >  1:1195491/2‑100 (MQ=255)
                                                                               cGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACcaca                      <  1:40045/100‑1 (MQ=255)
                                                                                gCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCacac                     <  1:679157/100‑1 (MQ=255)
                                                                                 cccTGAGAGGATCGTCTCCGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACt                    >  1:2109759/1‑100 (MQ=255)
                                                                                 cccGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACt                    >  2:2160298/1‑100 (MQ=255)
                                                                                   cGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTa                   <  1:705268/99‑1 (MQ=255)
                                                                                      agaCGATCCTCTCAGGGCATTTTTTTTTTTTTTTTTGTCAGAAACGATCGGCTCGCTGGAACTAGCGCTTTTGGGTTCTTTATTTAAGCAACACTAActg               >  1:113212/1‑100 (MQ=255)
                                                                                         cGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATctgctg            <  1:322202/100‑1 (MQ=255)
                                                                                         cGATCCTCTCAGGGCATTTTTGATATGGGTACTGTAAGAATCCATCGGCGCTCTGGCACAGGAGCAGCTGGGATCGCTATTTAAACCACACTCTctgctg            >  1:914346/1‑100 (MQ=255)
                                                                                          gATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCtgctgc           <  2:212843/100‑1 (MQ=255)
                                                                                               tctcAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCTGCTGCGCCAt      >  2:233090/1‑100 (MQ=255)
                                                                                                   aGGGCATTTTTGATATGTGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCTGCTGCGCCATCGCa  <  2:1579166/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTGTTGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCTGCTGCGCCATCGCA  >  NC_000913/2143626‑2143824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: