Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 217,037 A→T 20.1% intergenic (‑34/+20) yaeF ← / ← proS putative lipoprotein/prolyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913217,0370AT20.1% 313.8 / 70.1 139intergenic (‑34/+20)yaeF/proSputative lipoprotein/prolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (60/51);  new base T (18/10);  total (78/61)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

Only 100 of 144 total aligned reads displayed.

GTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATT  >  NC_000913/216939‑217136
                                                                                                  |                                                                                                   
gTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAAt                                                                                                    >  2:1091743/1‑100 (MQ=255)
gTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAAt                                                                                                    <  2:1800499/100‑1 (MQ=255)
  aCAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAAt                                                                                                    <  1:2113230/98‑1 (MQ=255)
   cAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCa                                                                                                 >  1:631372/1‑100 (MQ=255)
   cAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTAAGCATTTTATACAATCCATGCAAAACAAGGGACCTAGAATTCa                                                                                                 >  1:230429/1‑100 (MQ=255)
    aaGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGGAGTACGCCTTTGGTTTATCCATTTTATACAATACATGTAAAAAAAGGGCCCTGAAATTCAg                                                                                                <  1:1316301/100‑1 (MQ=255)
    aaGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAATTTCa                                                                                                 <  1:2871390/99‑1 (MQ=255)
    aaGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAg                                                                                                <  2:2328583/100‑1 (MQ=255)
    aaGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAg                                                                                                <  1:1718843/100‑1 (MQ=255)
    aaGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAg                                                                                                <  2:2014751/100‑1 (MQ=255)
    aaGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAg                                                                                                <  2:1839083/100‑1 (MQ=255)
    aaGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTGTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAg                                                                                                <  1:2279208/100‑1 (MQ=255)
     aGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAgg                                                                                               >  2:2359431/1‑100 (MQ=255)
     aGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAgg                                                                                               >  2:1028577/1‑100 (MQ=255)
      gCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGa                                                                                              >  2:782472/1‑100 (MQ=255)
      gCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGa                                                                                              >  1:2077402/1‑100 (MQ=255)
        cGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGAcc                                                                                            <  1:2616628/100‑1 (MQ=255)
         gAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGAccc                                                                                           >  1:850680/1‑100 (MQ=255)
            aGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCttt                                                                                        >  2:738040/1‑100 (MQ=255)
             gTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTc                                                                                       >  1:1843157/1‑100 (MQ=255)
              tAAAAGAGAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCt                                                                                      >  2:1524381/1‑100 (MQ=255)
               aaaaGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAGAAAAAGGGGCCTGAAATTCAGGACCCTTTCt                                                                                      >  1:2702043/1‑99 (MQ=255)
                aaagaaagCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTgg                                                                                    >  1:1056320/1‑100 (MQ=255)
                 aagaaagCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTgg                                                                                    <  1:1234627/99‑1 (MQ=255)
                  ataaagCTGCGGAGTAACAGGCGGCAGTACGCCTTTGGTTTACCCATTTTATACAATCCATGTAAAAAAAGGGTCCTGAATTTCAGGGCCCttttttt                                                                                    <  1:2656991/96‑5 (MQ=25)
                  agaaagCTGGGGAGAAGCAGGCGGCAGTACGCCTTTGGTTTATCCATCTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGc                                                                                   >  1:1675522/1‑99 (MQ=255)
                  agaaagCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGCAATTCAGGACCCTTTCTGGCa                                                                                  >  1:891125/1‑100 (MQ=255)
                  agaaagCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCa                                                                                  >  2:1764542/1‑100 (MQ=255)
                     aagCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGTCCTGAATTTCAGGGCCCtttttttacatg                                                                               <  2:1993550/100‑10 (MQ=25)
                      agCTTGGGAGTAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGTCCTGAATTTCAGGGCCCtttttttacat                                                                                <  1:2379431/98‑9 (MQ=25)
                      agCTGTGGTGAAACAGGCGGCAGTACGCCTTTGTTTTGTCCATTTTATACAATCCATGTAAAAAAAGGGTCCTGAATTTCAGGGCCCtttttttaca                                                                                 <  1:2811081/97‑8 (MQ=21)
                       gCTGGGGAGAAACAGGTGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGAGCCTTTCTGGCATCAGc                                                                             >  1:1700068/1‑100 (MQ=255)
                        cTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGcc                                                                            <  2:2565272/100‑1 (MQ=255)
                        cTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGcc                                                                            >  1:2060995/1‑100 (MQ=255)
                            ggAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCttt                                                                         <  1:2763597/99‑1 (MQ=255)
                            ggAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTa                                                                        >  1:2319598/1‑100 (MQ=255)
                              agaAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAAt                                                                      <  1:2247282/100‑1 (MQ=255)
                              agaAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAAt                                                                      <  2:1289835/100‑1 (MQ=255)
                               gaAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATc                                                                     >  2:64492/1‑100 (MQ=255)
                               gaAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATc                                                                     >  1:1512777/1‑100 (MQ=255)
                                aaaCAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATGCAATCCATGTAAAAAAAGGTCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCt                                                                    <  1:1588301/100‑1 (MQ=255)
                                   cAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGtt                                                                 >  2:1444021/1‑100 (MQ=255)
                                     ggcggcAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTc                                                               >  1:2543098/1‑100 (MQ=255)
                                     ggcggcAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTc                                                               >  1:253841/1‑100 (MQ=255)
                                     ggcggcAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTc                                                               >  1:994971/1‑100 (MQ=255)
                                     ggcggcAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTc                                                               <  2:2397200/100‑1 (MQ=255)
                                      gcggcAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCa                                                              <  2:1105934/100‑1 (MQ=255)
                                       cggcAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCAc                                                             <  2:497040/100‑1 (MQ=255)
                                           aGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGa                                                         <  2:2375244/100‑1 (MQ=255)
                                            gTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGat                                                        >  2:2638986/1‑100 (MQ=255)
                                             tACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGata                                                       >  2:1627303/1‑100 (MQ=255)
                                                gCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATAtt                                                     >  2:2368315/1‑99 (MQ=255)
                                                 ccTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTcg                                                   >  1:837537/1‑100 (MQ=255)
                                                  cTTTGGTTTATCCATTTTATACAATCCATTTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTcg                                                   <  1:464296/99‑1 (MQ=255)
                                                  cTTTGGTGTATACATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGACATCAGCCTTTAATCTGTTTCACCAGATATTcga                                                  >  1:1526047/1‑100 (MQ=255)
                                                   tttGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTcgac                                                 <  1:806165/100‑1 (MQ=255)
                                                    ttGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTcgacg                                                <  2:2913468/100‑1 (MQ=255)
                                                    ttGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTcgacg                                                <  1:171705/100‑1 (MQ=255)
                                                       gTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATg                                             >  2:1193082/1‑100 (MQ=255)
                                                           aTCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCAc                                         <  1:2018688/100‑1 (MQ=255)
                                                           aTCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCAc                                         <  2:354363/100‑1 (MQ=255)
                                                             ccATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCa                                       >  2:2023657/1‑100 (MQ=255)
                                                             ccATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCa                                       <  2:1321170/100‑1 (MQ=255)
                                                                   tataCAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATTTTCGACGATGTCACCAGTCtta                                 >  1:2114286/1‑100 (MQ=255)
                                                                      aCAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCttaatt                              <  2:2741466/100‑1 (MQ=255)
                                                                        aaTCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCttaattaa                            >  1:574072/1‑100 (MQ=255)
                                                                        aaTCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTAACCAGATATTCGACGATGTCACCAGTCttaattaa                            <  1:1162380/100‑1 (MQ=255)
                                                                         aaccAAGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAAc                           <  1:1324089/98‑1 (MQ=255)
                                                                         aTCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAAc                           >  2:2367854/1‑100 (MQ=255)
                                                                           ccATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTg                         >  2:2254914/1‑100 (MQ=255)
                                                                            tgatgccagaaaGGGTCCTGAATTTCAGGGCCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCTACGATGTCACCAGTCTTAATTAACTGt                        >  2:2743946/10‑100 (MQ=25)
                                                                            cATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGt                        <  2:419340/100‑1 (MQ=255)
                                                                            atgtaaaaaaaaGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGt                        >  1:2265453/6‑100 (MQ=255)
                                                                             aTGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGtt                       >  1:1942117/1‑100 (MQ=255)
                                                                              tGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGttt                      >  1:677245/1‑100 (MQ=255)
                                                                              atgccagaaaGGGTCCTGAATTTCTGGGTCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGt                        <  1:2743928/91‑1 (MQ=37)
                                                                                tAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTtct                    >  1:2634020/1‑100 (MQ=255)
                                                                                gccaggaaGGGTCCTGAATTTCAGGGCCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTtct                    >  1:2889866/7‑100 (MQ=38)
                                                                                 ccagaaaGGGTCCTGAATTTCAGGGCCCTTTCTGGCATCAGCCTTTAATCTGTTTCTCCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTtctc                   >  1:2394747/5‑100 (MQ=37)
                                                                                 ccagaaaGGGTCCTGAATTTCAGGGCCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTtctc                   >  2:2122077/5‑100 (MQ=38)
                                                                                 ccagaaaGGGTCCTGAATTTCAGGGCCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTtctc                   >  1:1500498/5‑100 (MQ=38)
                                                                                 ccagaaaGGGTCCTGAATTTCAGGGCCCGTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTtctc                   >  1:1821260/5‑100 (MQ=38)
                                                                                 aaaaaaaGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTtctc                   >  2:767730/1‑100 (MQ=255)
                                                                                 aaaaaaaGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTtc                     >  2:312239/1‑98 (MQ=255)
                                                                                  aaaaaaGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCg                  <  1:2359416/100‑1 (MQ=255)
                                                                                      aaGGGTCCTGAATTTCAGGGCCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGt              >  1:1956340/1‑100 (MQ=39)
                                                                                      aaGGGTCCTGAATTTCAGGGCCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGt              >  1:1640725/1‑100 (MQ=39)
                                                                                      aaGGGTCCTGAATTTCAGGGCCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGt              >  2:1875640/1‑100 (MQ=39)
                                                                                       aGGGTCCTGAATTTCAGGGCCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGtt             >  1:1429058/1‑100 (MQ=255)
                                                                                       aGGGTCCTGAATTTCAGGGCCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGtt             >  2:1856036/1‑100 (MQ=255)
                                                                                        gggTCCTGAATTTCAGGGCCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGGTATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTg            >  2:1239326/1‑100 (MQ=255)
                                                                                        gggTCCTGAATTTCAGGGCCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTg            >  1:1469389/1‑100 (MQ=255)
                                                                                        gggCCCTGAAATGCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATACGACGATGTCACCAGTCTTAATTAACTGGTTCTCGCCGTTg            <  1:1592453/100‑1 (MQ=255)
                                                                                         ggCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTCATTAACTGTTTCTCGCCGTTGc           <  1:1693607/100‑1 (MQ=255)
                                                                                          gCCCTGACATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCCTAATTAACTGTTTCTCGCCGTTGc           <  1:428001/99‑1 (MQ=255)
                                                                                            ccTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGcgac        <  1:1874993/100‑1 (MQ=255)
                                                                                              tGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGCCTTAATTAACTGTTTCTCCCCGTTGcgacga      >  1:1875794/1‑100 (MQ=255)
                                                                                               gAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGat     >  1:246749/1‑100 (MQ=255)
                                                                                                aaaTTCAGGACACTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCATAATTAACTGTTTCTCGCCGTTGCGACGata    <  1:780866/100‑1 (MQ=255)
                                                                                                 taTTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGatat   <  1:1733977/99‑1 (MQ=255)
                                                                                                  |                                                                                                   
GTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATT  >  NC_000913/216939‑217136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: