Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,314,800 A→C 17.4% Q438P (CAG→CCG) ‡ rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,314,8000AC17.4% 256.5 / 53.8 116Q438P (CAG→CCG) rcsDphosphotransfer intermediate protein in two‑component regulatory system with RcsBC
Reads supporting (aligned to +/- strand):  ref base A (48/47);  new base C (9/11);  total (58/58)
Fisher's exact test for biased strand distribution p-value = 8.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01

Only 100 of 117 total aligned reads displayed.

GGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGA  >  NC_000913/2314702‑2314899
                                                                                                  |                                                                                                   
ggggATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAg                                                                                                    <  1:1020813/100‑1 (MQ=255)
  ggATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGa                                                                                                  >  2:1709896/1‑100 (MQ=255)
  ggATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGa                                                                                                  >  2:2782259/1‑100 (MQ=255)
  ggATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGa                                                                                                  >  1:1641258/1‑100 (MQ=255)
   gATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGAt                                                                                                 >  2:1249668/1‑100 (MQ=255)
       attCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATcgcg                                                                                             <  1:470311/100‑1 (MQ=255)
          cAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAACTTTTCATTATTCGCGATCAGGATCGCGAAg                                                                                          >  1:544740/1‑100 (MQ=255)
          cAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaat                                                                                          <  1:1102344/100‑2 (MQ=255)
          cAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaat                                                                                          <  2:2109846/100‑2 (MQ=255)
          cAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaat                                                                                          <  2:2191722/100‑2 (MQ=255)
          cAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAg                                                                                          >  1:1763600/1‑100 (MQ=255)
          cAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAg                                                                                          >  1:1842550/1‑100 (MQ=255)
           aGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaata                                                                                         <  2:1947520/100‑3 (MQ=255)
           aGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGt                                                                                         <  1:2129273/100‑1 (MQ=255)
             gCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaataat                                                                                       <  2:806104/100‑5 (MQ=255)
              cgacgaTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaataatg                                                                                      <  2:1501011/100‑6 (MQ=255)
              cgacgaTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATCCGCGATCCTGATCGCGaataatg                                                                                      <  2:199357/100‑6 (MQ=255)
                acgtTCAATAACGAGCTGTTTGCGATCCGACTGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaataatgaa                                                                                    <  1:2085532/100‑8 (MQ=25)
                acgaTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaataatgaa                                                                                    <  1:369469/100‑8 (MQ=38)
                   aTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTaa                                                                                 <  1:1248861/100‑1 (MQ=255)
                   aTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTaa                                                                                 >  2:284749/1‑100 (MQ=255)
                   aTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTaa                                                                                 >  2:469059/1‑100 (MQ=255)
                     cAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAAc                                                                               <  1:2333405/100‑1 (MQ=255)
                      aaTAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACa                                                                              <  2:924104/100‑1 (MQ=255)
                      aaTAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACa                                                                              <  1:1626556/100‑1 (MQ=255)
                      aaTAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGAATCGCGAAGTGCTGGTAAACa                                                                              >  2:467381/1‑100 (MQ=255)
                       aTAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACaa                                                                             >  2:2005557/1‑100 (MQ=255)
                       aTAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACaa                                                                             <  2:876917/100‑1 (MQ=255)
                        tAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAg                                                                            <  1:1635019/100‑1 (MQ=255)
                          aCGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGaa                                                                          <  1:1898010/100‑1 (MQ=255)
                          aCGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGaa                                                                          >  1:901847/1‑100 (MQ=255)
                            gAGCTGTTTGAGCTCTGCATGTTGCCCAGTCAGGTCTCGCCTCGCACACAAGTTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGaaat                                                                        <  1:1182063/100‑2 (MQ=255)
                            gAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAAc                                                                        <  2:1557832/100‑1 (MQ=255)
                            gAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAAc                                                                        <  2:1268336/100‑1 (MQ=255)
                                tGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCaa                                                                    >  1:632618/1‑100 (MQ=255)
                                tGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCaa                                                                    <  1:1155553/100‑1 (MQ=255)
                                 gTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAg                                                                   >  2:1008029/1‑100 (MQ=255)
                                   aTGAGATCCGCATGTTCCGCAGCCAGGGCGCGCCGCGCACACACATTTTCATTATTCGCGCTCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCa                                                                 <  1:1302947/100‑1 (MQ=255)
                                      agaTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGgc                                                              >  2:2711104/1‑100 (MQ=255)
                                      agaTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGgc                                                              <  2:314273/100‑1 (MQ=255)
                                      agaTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGgc                                                              >  1:939904/1‑100 (MQ=255)
                                       gaTCCGCATGTTCCGCAGCTAGGTCGCGCGGCGCACACAAATGTTCATTATTCGCGATCGGGAGAGCGACGTGCTGGTACACAAGAAAATCAAg                                                                   >  1:963728/1‑94 (MQ=255)
                                       gaTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGgcg                                                             >  1:564217/1‑100 (MQ=255)
                                       gaTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGgcg                                                             >  2:195890/1‑100 (MQ=255)
                                         tCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCa                                                           >  2:939612/1‑100 (MQ=255)
                                           cGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCTCAGc                                                         >  2:1021324/1‑100 (MQ=255)
                                           cGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGc                                                         >  1:2547468/1‑100 (MQ=255)
                                            gCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCg                                                        <  2:456193/100‑1 (MQ=255)
                                            gCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCACGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCg                                                        >  1:2136028/1‑100 (MQ=255)
                                             cATGTTCCGCCGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCTCGATCAGGATCCCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCg                                                        >  1:2375519/1‑99 (MQ=255)
                                               tGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCt                                                     <  2:1913450/100‑1 (MQ=255)
                                               tGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCt                                                     >  1:2586294/1‑100 (MQ=255)
                                                gTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTg                                                    <  2:2165783/100‑1 (MQ=255)
                                                gTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTg                                                    <  2:1131171/100‑1 (MQ=255)
                                                  tCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTa                                                  <  1:2665956/100‑1 (MQ=255)
                                                  tCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTa                                                  <  2:1407596/100‑1 (MQ=255)
                                                  tCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTa                                                  >  1:146917/1‑100 (MQ=255)
                                                  tCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTa                                                  >  2:2289494/1‑100 (MQ=255)
                                                   ccGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTAt                                                 >  1:1496110/1‑100 (MQ=255)
                                                   ccGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTAt                                                 <  1:497005/100‑1 (MQ=255)
                                                    cGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATg                                                <  2:1425074/100‑1 (MQ=255)
                                                     gCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATga                                               >  2:634592/1‑100 (MQ=255)
                                                         ccAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGaaa                                           <  2:2023861/100‑1 (MQ=255)
                                                          cAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGaaaa                                          <  1:2182080/100‑1 (MQ=255)
                                                          cAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGaaaa                                          >  2:1730163/1‑100 (MQ=255)
                                                           aGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGaaaaa                                         <  2:2332892/100‑1 (MQ=255)
                                                            ggTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAAc                                        >  1:923738/1‑100 (MQ=255)
                                                             gTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAAcc                                       <  2:317586/100‑1 (MQ=255)
                                                                gcgccgcgcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACcagc                                    >  1:2058236/1‑100 (MQ=255)
                                                                 cgccgcgcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACcagca                                   <  2:179759/100‑1 (MQ=255)
                                                                   ccgcgcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAgg                                 <  2:520619/100‑1 (MQ=255)
                                                                       gcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCg                             <  2:1898258/100‑1 (MQ=255)
                                                                       gcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCg                             >  1:2568309/1‑100 (MQ=255)
                                                                       gcACACAAATTTTCATTATTCGCCATCAGGATCGCGAAGTAATGGTAAACAAGAATCTCAAGCATGCGCAGCGAAAAAAAAAAAAAAACCAGCAGGGGCg                             <  1:2550647/100‑1 (MQ=255)
                                                                        cacacaAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCgg                            <  1:1435290/100‑1 (MQ=255)
                                                                                 tttCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCttt                   >  2:1794481/1‑100 (MQ=255)
                                                                                  ccagcacttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGCCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTa                  >  1:679452/8‑100 (MQ=38)
                                                                                   tCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTAt                 >  1:1926976/1‑100 (MQ=255)
                                                                                    cATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTAt                 >  1:1868616/1‑99 (MQ=255)
                                                                                    cATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATg                <  2:1235823/100‑1 (MQ=255)
                                                                                    cATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATg                <  2:2857437/100‑1 (MQ=255)
                                                                                    cATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGAACTTTATg                >  1:389940/1‑100 (MQ=255)
                                                                                       tattCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaa             >  1:1822504/1‑100 (MQ=255)
                                                                                        cttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCGAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaa            >  1:2822934/2‑100 (MQ=255)
                                                                                        cttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaa            >  1:1681145/2‑100 (MQ=255)
                                                                                        cttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaa            >  1:1840051/2‑100 (MQ=255)
                                                                                        cttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaa            >  1:2059688/2‑100 (MQ=255)
                                                                                        cttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaa            >  2:1475760/2‑100 (MQ=255)
                                                                                        attCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaa            >  2:1082664/1‑100 (MQ=255)
                                                                                         ttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaaa           >  2:2248658/1‑100 (MQ=255)
                                                                                         ttCGCGATCCTGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaaa           >  1:434741/1‑100 (MQ=255)
                                                                                         ttCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaaa           >  1:2466179/1‑100 (MQ=255)
                                                                                          tCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAAc          >  2:2365678/1‑100 (MQ=255)
                                                                                           cgcgATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACa         >  2:2459945/1‑100 (MQ=255)
                                                                                             cgATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACAtt       >  1:369142/1‑100 (MQ=255)
                                                                                              gATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGTAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTg      <  2:1679859/100‑1 (MQ=255)
                                                                                              gATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTg      <  1:1987039/100‑1 (MQ=255)
                                                                                                 cAGGATCGCGAAGTGCTGGTAAACAATTACCTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCATGGGCGTATGATCTTTATGAAAAACATTGGCg   <  1:752416/100‑1 (MQ=255)
                                                                                                 cAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAACCATTGGc    <  2:2446646/99‑1 (MQ=255)
                                                                                                  aGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGa  >  1:658700/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
GGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGA  >  NC_000913/2314702‑2314899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: