Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,391,417 C→G 18.2% W30S (TGG→TCG)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,391,4170CG18.2% 277.2 / 58.2 115W30S (TGG→TCG) nuoNNADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base C (40/54);  new base G (10/11);  total (50/65)
Fisher's exact test for biased strand distribution p-value = 8.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.80e-01

Only 100 of 120 total aligned reads displayed.

ACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACG  >  NC_000913/2391318‑2391516
                                                                                                   |                                                                                                   
aCGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGcc                                                                                                     <  2:1498007/100‑1 (MQ=255)
 cGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCa                                                                                                    <  1:101534/100‑1 (MQ=255)
 cGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCa                                                                                                    >  1:2746851/1‑100 (MQ=255)
  gTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCAc                                                                                                   >  2:145061/1‑100 (MQ=255)
    ccATAGCGCCCGCCTGGCCATCAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGGAGCGTAGAGGAAAGGATTGGGTCGCCACg                                                                                                  >  1:1782321/1‑99 (MQ=255)
    ccATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTCAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCa                                                                                                    >  1:2521784/1‑97 (MQ=255)
     cATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCa                                                                                                <  1:166971/100‑1 (MQ=255)
       tAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAAt                                                                                              >  2:2445288/1‑100 (MQ=255)
        aGCGCCCGCCTGGCCAATAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtc                                                                                             <  2:2714280/100‑2 (MQ=255)
        aGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAAt                                                                                              <  1:1848448/99‑1 (MQ=255)
        aGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATg                                                                                             >  2:2747522/1‑100 (MQ=255)
         gcgcCCGCCTTGCCAACAAACCAGAGAGGACGCGGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGTAAATGATTGCGTCGACACGCAATgg                                                                                            <  1:2494610/100‑1 (MQ=255)
         gcgcCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATgg                                                                                            >  2:1179659/1‑100 (MQ=255)
         gcgcCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATgg                                                                                            <  1:1179654/100‑1 (MQ=255)
          cgcccgccTGGCCAATAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGCTGAGGAAATGATTGCGTCGCCACGCAATgg                                                                                            >  1:2418017/1‑99 (MQ=255)
           gcccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGgag                                                                                          <  1:1581355/100‑1 (MQ=255)
           gcccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGCTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGgag                                                                                          >  1:179818/1‑100 (MQ=255)
            cccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcattt                                                                                         <  2:940678/100‑6 (MQ=255)
            cccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatt                                                                                          <  2:2582428/99‑5 (MQ=255)
            ccccccTGGCCAACAAGCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcattt                                                                                         <  1:2668516/100‑6 (MQ=255)
             ccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCTCGCAATGGagag                                                                                        <  2:1922848/100‑1 (MQ=255)
             ccgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGagag                                                                                        <  1:815762/100‑1 (MQ=255)
              cgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcc                                                                                       <  2:1502810/100‑8 (MQ=255)
              cgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcc                                                                                       <  2:2131054/100‑8 (MQ=255)
              cgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcc                                                                                       <  2:2397546/100‑8 (MQ=255)
              cgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGc                                                                                       <  1:2807173/100‑1 (MQ=255)
              cgccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGc                                                                                       <  2:987289/100‑1 (MQ=255)
               gccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTGGCGACGCAAtcatttcc                                                                                       <  2:1124143/99‑8 (MQ=38)
               gccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCa                                                                                      <  2:639911/100‑1 (MQ=255)
               gccTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGATAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCa                                                                                      >  2:2053369/1‑100 (MQ=255)
                 cTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATc                                                                                    <  2:2836224/100‑1 (MQ=255)
                 cTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGACGCCACGCAATGGAGAGCATc                                                                                    >  1:339233/1‑100 (MQ=255)
                  tGGCCAATAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATca                                                                                   <  1:1258195/100‑1 (MQ=255)
                  tGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATca                                                                                   <  2:666032/100‑1 (MQ=255)
                  tGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATca                                                                                   <  1:1020220/100‑1 (MQ=255)
                   ggCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATcac                                                                                  <  1:994288/100‑1 (MQ=255)
                    gCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATcaca                                                                                 >  2:90425/1‑100 (MQ=255)
                    gCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATcaca                                                                                 <  1:2865316/100‑1 (MQ=255)
                      caataaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGAAAATGATTGCGACGCCACGCAATGGAGAGCa                                                                                      >  1:2906280/1‑93 (MQ=255)
                      caacaaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAac                                                                               >  1:1460201/1‑100 (MQ=255)
                      caacaaACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAac                                                                               >  1:557183/1‑100 (MQ=255)
                          aaaCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAaccacc                                                                           <  2:2550578/100‑1 (MQ=255)
                          aaaCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAaccacc                                                                           <  1:477603/100‑1 (MQ=255)
                            aCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAaccaccac                                                                         >  2:1496104/1‑100 (MQ=255)
                            aCCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAaccaccac                                                                         >  2:2348949/1‑100 (MQ=255)
                              cAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAaccaccaccn                                                                       <  2:1372159/100‑2 (MQ=255)
                                gagCGAAACCAGCGCCGCGTTAAGCCCAGTCACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAaccacc                                                                           <  1:1126217/94‑1 (MQ=255)
                                  gCGAAACCAGCGCCGCGTTAAGTCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTc                                                                     >  1:1513003/1‑98 (MQ=255)
                                  gCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCaa                                                                   >  2:1351331/1‑100 (MQ=255)
                                   cGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGCAGCGATGCGGAACTGACTGCGACGCCACGCACAGGAGAGCATCCCAACCGACACCGTCAAg                                                                  >  1:1268915/1‑100 (MQ=255)
                                    gAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGc                                                                 <  2:143512/100‑1 (MQ=255)
                                    gAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGc                                                                 <  2:48184/100‑1 (MQ=255)
                                    gAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGc                                                                 >  1:356980/1‑100 (MQ=255)
                                        ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgac                                                             <  1:1906048/100‑1 (MQ=255)
                                        ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCcgac                                                             <  2:1196090/100‑1 (MQ=255)
                                        ccAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAAAGCATCACAACCACCACCGTCAAGCcg                                                               >  1:1568778/1‑98 (MQ=255)
                                             gccgcGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCa                                                        <  2:1924504/100‑1 (MQ=255)
                                              ccgcGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAg                                                       >  2:1256669/1‑100 (MQ=255)
                                              ccgcGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAg                                                       >  2:1700794/1‑100 (MQ=255)
                                                 cgTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAAGCACCACCGTCAAGCCGACGATCAGCaa                                                    >  1:2669588/1‑100 (MQ=255)
                                                 cgTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCaa                                                    >  2:598371/1‑100 (MQ=255)
                                                      aGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTa                                               <  1:113678/100‑1 (MQ=255)
                                                       gCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAg                                              <  1:947468/100‑1 (MQ=255)
                                                        cccAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGc                                             <  1:1012345/100‑1 (MQ=255)
                                                        cccAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGc                                             <  2:1389191/100‑1 (MQ=255)
                                                        cccAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGc                                             <  1:2634054/100‑1 (MQ=255)
                                                         ccAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCa                                            <  1:2899648/100‑1 (MQ=255)
                                                          cAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAg                                           <  2:1784905/100‑1 (MQ=255)
                                                             tagccGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGc                                        <  2:557186/97‑1 (MQ=255)
                                                             tAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGc                                        <  2:2632366/100‑1 (MQ=255)
                                                             tAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGc                                        >  2:791341/1‑100 (MQ=255)
                                                               aCCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGCGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGa                                      >  1:2507644/1‑100 (MQ=255)
                                                               aCCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGa                                      <  1:1811142/100‑1 (MQ=255)
                                                                 cGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATc                                    <  1:778605/100‑1 (MQ=255)
                                                                  gagaGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATc                                    <  2:1655753/99‑1 (MQ=255)
                                                                   agagCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAg                                  <  2:1503651/100‑1 (MQ=255)
                                                                    gagCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAgg                                 <  1:2678603/100‑1 (MQ=255)
                                                                       cgtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGttt                              >  1:2133083/1‑100 (MQ=255)
                                                                        gtagcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGtttt                             <  1:2120559/100‑1 (MQ=255)
                                                                           gcgtTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTtgtg                          <  2:2650569/100‑1 (MQ=255)
                                                                                 aGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGtt                     >  1:914953/1‑99 (MQ=255)
                                                                                    tctccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTg                 >  2:1142291/6‑100 (MQ=255)
                                                                                    aaaTGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCCACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTg                 <  1:1617771/100‑1 (MQ=255)
                                                                                     ctccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTg                 >  2:335199/5‑99 (MQ=255)
                                                                                     ctccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGt                >  2:263753/5‑100 (MQ=255)
                                                                                       tGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa              >  1:2508386/1‑100 (MQ=255)
                                                                                       ccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa              >  2:2826490/3‑100 (MQ=255)
                                                                                       ccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCa              >  2:2841113/3‑100 (MQ=255)
                                                                                       ccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTCGAATTATTGTCa              >  1:2847087/3‑100 (MQ=255)
                                                                                       ccaTTGCGTGGCGACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGGTTTGTGGAGTTATTGTCa              >  1:2405592/3‑100 (MQ=255)
                                                                                        caTTGCGTGGCGACGCAATGCAGAGTATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGATTTGTGCAGttggtgt                >  1:1776779/2‑92 (MQ=255)
                                                                                         aTTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATg            >  2:2264701/1‑100 (MQ=255)
                                                                                         aTTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATg            <  1:2445273/100‑1 (MQ=255)
                                                                                         aTTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATg            <  1:550956/100‑1 (MQ=255)
                                                                                              gTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGAt       <  2:1663093/100‑1 (MQ=255)
                                                                                                 gCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGCCATGGCGATTTa    >  2:450409/1‑100 (MQ=255)
                                                                                                  ccACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTAc   >  2:1392949/1‑100 (MQ=255)
                                                                                                  ccACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTAc   <  2:874680/100‑1 (MQ=255)
                                                                                                  ccACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTAc   <  1:251880/100‑1 (MQ=255)
                                                                                                   cACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACg  >  2:179715/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
ACGTCCATAGCGCCCGCCTGGCCAACAAACCAGAGCGAAACCAGCGCCGCGTTAAGCCCAATAACCGAGAGCGTAGCGTTGAGGAAATGATTGCGTCGCCACGCAATGGAGAGCATCACAACCACCACCGTCAAGCCGACGATCAGCAACGGTAGCAGTGCGATCAGGTTTTGTGGAGTTATTGTCATGGCGATTTACG  >  NC_000913/2391318‑2391516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: